scholarly journals First Report on the Occurrence and Subtypes of Blastocystis in Pigs in Poland Using Sequence-Tagged-Site PCR and Barcode Region Sequencing

Pathogens ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 595
Author(s):  
Monika Rudzińska ◽  
Beata Kowalewska ◽  
Beata Szostakowska ◽  
Maciej Grzybek ◽  
Katarzyna Sikorska ◽  
...  
Keyword(s):  
Age Groups ◽  
Mixed Infections ◽  
Age Group ◽  
Blastocystis Infection ◽  
Pathogenic Role ◽  
Sequence Tagged Site ◽  
New Methods ◽  
Molecular Approaches ◽  
Stool Samples ◽  
Positive Results

Blastocystis is an enteric microorganism commonly found in humans and animals worldwide. Its pathogenic role in humans and transmission patterns has not been fully explained. However, nine subtypes (ST1–8, ST12) are considered as potentially zoonotic. Studies from various regions of the world show that pigs are mainly infected with ST5. Although pigs are important farmed animals in Poland, the question of Blastocystis infection in these animals has not yet been investigated. Herein, 149 pig stool samples from 10 Polish pig farms were analyzed using sequence-tagged-site PCR and barcode region sequencing. The percentage of samples in which Blastocystis was identified using each method separately was similar: 38.25% and 37.58%, respectively. However, the percentage of positive results obtained by combining both methods was 46.97%, which means that, depending on the method used, the number of undetected samples varied between 8.72% and 9.39%. This shows the methodological limitations of up-to-date molecular approaches commonly used in Blastocystis research. A moderate infection rate (44.4–50%) observed in different pig age groups with a vital predominance of ST5 (94.28%) in every age group shows that pigs are a likely natural host of ST5. A small percentage of mixed infections, namely ST5/ST1 (5.26%), ST5/ST3 (1.75%), and ST3/ST1 (1.75%), was observed only in animals of older age, suggesting that ST3 and ST1 can be acquired by pigs during contact with humans. This study provides the first data on the prevalence and Blastocystis subtypes (STs) distribution in pigs in Poland. The results also highlight the need for the development of new methods capable of detecting highly genetically diverse Blastocystis isolates and mixed infections.

scholarly journals GAMBARAN BASIL TAHAN ASAM (BTA) POSITIF PADA PENDERITA DIAGNOSA KLINIS TUBERKULOSIS PARU DI RUMAH SAKIT ISLAM SITTI MARYAM MANADO PERIODE JANUARI 2014 S/D JUNI 2014

2014 ◽  
Vol 2 (2) ◽  
Author(s):  
Velma Buntuan
Keyword(s):  
Infectious Disease ◽  
Experimental Study ◽  
Mycobacterium Tuberculosis ◽  
Age Groups ◽  
Microscopical Examination ◽  
Age Group ◽  
Negative Results ◽  
Lung Tuberculosis ◽  
Tuberculosis Diagnosis ◽  
Positive Results

Absract: Tuberculosis is an acute or chronic infectious disease caused by Gram positive bacil, Mycobacterium tuberculosis, which predominantly infect the lungs. The diagnosis of lung tuberculosis in adults is based on the finding of these mycobacyteria in the microscopical examination of sputum. This was a descriptive experimental study to obtain the mycobacteria in patients’ sputum stained with Ziehl Neelsen method. Subjects were outpatients and inpatients clinically diagnosed as tuberculosis at the Internal Department of Sitti Maryam Hospital in Manado. The results showed that of 87 samples, there were 48 males (55%) and 39 females (45%). Age groups of 0-15 years consisted of 1 sample (1%); 16-20 years 0 sample (0%); 21-60 years 79 samples (91%); and >60 years 7 samples (8%). The microscopical examination showed positive results in 27 samples (37.0%) and negative results in 60 samples (69.0%). Conclusion: Mycobacterium tubercuosis infections were more frequent in males than in females. The most frequent age group was productive age, and the least frequent ages were children and teenagers. The number of positive results of sputum examination for Mycobacterium tuberculosis were less than negative results.Keywords: Mycobacterium tuberculosis, lung tuberculosis.Abstrak: Tuberkulosis paru adalah penyakit yang menular akut maupun kronis yang terutama menyerang paru, yang disebabkan oleh bakteri tahan asam (BTA) berbentuk batang yang bersifat Gram positif (Mycobacterium tuberculosis). Diagnosis tuberkulosis paru pada dewasa dapat ditegakkan dengan ditemukan BTA pada pemeriksaan mikroskopik dahak. Penelitian ini bersifat deskriptif ekperimental untuk menemukan kuman Mycobacterium tuberculosis pada sputum penderita dengan diagnosa klinis tuberkulosis di Poliklinik dan Rawat Inap Penyakit Dalam RSI Sitti Maryam Manado. Teknik pewarnaan BTA menggunakan metode Ziehl Neelsen. Hasil penelitian memperlihatkan dari 87 sampel, 48 sampel (55%) berjenis kelamin laki-laki dan 39 sampel (45%) berjenis kelamin perempuan. Jumlah sampel untuk kelompok usia 0-15 tahun sebesar 1 sampel (1%); 16-20 tahun 0 sampel (0%); 21-60 tahun 79 sampel (91%); dan >60 tahun 7 sampel (8%). Pada pemeriksaan sputum ditemukan BTA pada 27 sampel (37,0%) sedangkan pada 60 sampel (69,0%) tidak ditemukan. Simpulan: Pengidap tuberkulosis berjenis kelamin laki-laki lebih banyak dari pada perempuan, dengan kelompok usia tersering pada usia produktif dan paling sedikit pada usia anak dan remaja. Pemeriksaan sputum BTA positip lebih sedikit dibandingkan dengan BTA Negatip.Kata kunci: BTA, tuberkulosis paru.

scholarly journals Clinical characteristics of rotaviruses disease

2004 ◽  
Vol 4 (2) ◽  
pp. 22-24 ◽  
Author(s):  
Šukrija Zvizdić ◽  
Sađida Telalbašić ◽  
Edina Bešlagić ◽  
Semra Čavaljuga ◽  
Jasminka Maglajlić ◽  
...  
Keyword(s):  
Young Children ◽  
Wide Spectrum ◽  
Age Groups ◽  
Clinical Signs ◽  
Signs And Symptoms ◽  
Latex Agglutination ◽  
Hospitalized Patients ◽  
Viral Gastroenteritis ◽  
Age Group ◽  
Stool Samples

Rotaviruses are the major causes of viral gastroenteritis in infant and the young children. There are a wide spectrum of clinical signs and symptoms of rotavirosis. Rotavirus infection is fecal-oral infection. Rotaviruses prove with Latex agglutination test and electron microscopy. In a four year period 943 stool samples out of 527 hospitalized patients had been analyzed. A presence of rotavirus is proved with a LA and EM tests at 170 (32,2%) patients age 0-7 years, in their stool samples. Analyzing age groups of these patients, it was found that the rotavirusesinfection the most frequently occurred at age group from 7-24 months. From 170 positive patients, 122 or 71,8% were in this age group. At all patients was found diarrhea, vomiting in a 90,5% cases. Mild fever had 65,5% patients, signs of a respiratory infection appeared at 60,7% patients, abdominal pain at 13,3% patients. Severe dehydration had 49,9% patients and metabolic acidosis had 79,2% cases. Macroscopically blood in stool had 6,4% patients, slime in a stool 46,0% patients was found, and aholic stool had 8,4% patients. In all hospitalized patients disease lasted in average 12,6 days, and the hospitalization in average 10,2 days. None of the patient had any kind of complication, all of them very successfully cured. These results confirm that rotaviruses are important health problem among infant and the young children in Bosnia and Herzegovina.

scholarly journals Detection of Enteric Bacterial Pathogens (Vibrio Cholerae and Escherichia Coli O157) in Childhood Diarrhoeal Cases

1970 ◽  
Vol 5 (5) ◽  
pp. 23-26 ◽  
Author(s):  
Roshani Maharjan ◽  
Binod Lekhak ◽  
Chandrika Devi Shrestha ◽  
Jyotsna Shrestha
Keyword(s):  
Vibrio Cholerae ◽  
Tap Water ◽  
Age Groups ◽  
Oral Rehydration Therapy ◽  
Age Group ◽  
Cross Sectional ◽  
Remarkable Feature ◽  
Oral Rehydration ◽  
E Coli ◽  
Stool Samples

A hospital based cross sectional study was carried out in stool samples collected from cases of diarrhoea in children admitted to Oral Rehydration Therapy (ORT) ward, Kanti Children Hospital, Maharajgung. A total of 204 stool samples collected from children below 15 years were processed at Department of Microbiology, Bir hospital during the study period, February 2004 to June 2004.The stool specimens were investigated for Vibrio cholerae, as well as E. coli O157. Bloody stools were more focused for isolation of E. coli O157. Out of 204 patients, 60.3% were male and 39.7% were female. The largest number of diarrhoeal patients belong to age group 0-5 years i.e. 112 (54.9%). Vibrio cholerae O1 was found in 86 (42.2%) cases. All V. cholerae O1 belong to Ogawa serovar and El Tor biotype. Out of 86 isolates, 52.3% were from male patient and 47.7% were from female patient. Highest incidence of V. cholerae O1 was found in age groups 5-10 (46.5%) Isolation of V. cholerae in 10 cases even in age group 0-2 was remarkable feature. Incidence of V. cholerae O1 was highest in the month of April (61.5%). E. coli O157 could not be detected in this study. Predominant or pure growth of sorbitol non fermenting (SNF) strains which were biochemically identified as E. coli but not agglutinated with E.coli antiserum were found in five cases. Tetracycline was 100 percent effective antibiotic followed by Norfloxacin and Ciprofloxacin to V. cholerae O1. In this study, patients suffering with cholera were mostly from Kalanki. People using municipal tap water were mostly affected (51.8%) Out of 30 isolates of processed, 22 isolates showed toxin production. Key words: Vibrio cholerae; E. coli O157; Sorbital non fermenting; Cholera toxin. DOI: 10.3126/sw.v5i5.2651 Scientific World, Vol. 5, No. 5, July 2007 23-26

scholarly journals Detection of infection by rubella and cytomegalovirus among women exposed to abortion in Ninavah province

2019 ◽  
Vol 24 (1) ◽  
pp. 52
Author(s):  
Mahmood Nafi Mahmood1 ◽  
Adeeba Younis Shareef2
Keyword(s):  
Infected Patient ◽  
Age Groups ◽  
Control Group ◽  
Age Group ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Igg Antibody ◽  
High Level ◽  
Common Infection ◽  
Positive Results

The study aims to correlate infection with rubella, cytomegalovirus and abortion at different age groups. Blood samples were collected through the period between beginning of February to the end of September 2017, one hundred and twenty-seven women exposed to abortion and thirty apparently health controls. Serum samples were tested for IgM and IgG antibodies for rubella and cytomegalovirus. The results showed that CMV was the most common infection with high level of IgM antibodies (15.7%) and IgG (12.5%), only 0.7% of the cases gave positive results for rubella IgM, and 6.2% had rubella IgG while the control group only 6.6% had CMV IgM, and 3.3% had CMV IgG antibody. All the age groups did not show infection with rubella except age 25-31 years as 2.1% and 8.5% had IgM and IgG respectively. The highest rate of infection with CMV was at age group 25-31 years (21.2%) then age group 18-24 years (13.7%). Most of the patients experienced two abortions were reported at age group 25-31 years, 1.5% and 4.7% of CMV infected patient experienced 3 and 2 respectively while 1.5% of rubella infected patients had only one abortion, 18.1% of the patients with CMV experienced 2 abortions had cut off >1.25. Hundred % of the patients with rubella had cut off value of > 1.25 experienced one abortions.   http://dx.doi.org/10.25130/tjps.24.2019.008  

scholarly journals Age-Dependent Accuracy of Helicobacter pylori Antibody Assays for Adults, with Special Emphasis on Atrophic Gastritis

2004 ◽  
Vol 11 (6) ◽  
pp. 1185-1188 ◽  
Author(s):  
A. Salomaa-Räsänen ◽  
T. U. Kosunen ◽  
J. Mattila ◽  
S. Sarna ◽  
H. Rautelin
Keyword(s):  
Helicobacter Pylori ◽  
Atrophic Gastritis ◽  
Immunoglobulin G ◽  
False Positive ◽  
Age Groups ◽  
Age Group ◽  
Age Dependent ◽  
Antibody Assays ◽  
Positive Results ◽  
Helicobacter Pylori Antibody

ABSTRACT The accuracy of Helicobacter pylori antibody assays for 561 consecutive adult outpatients who had undergone gastroscopy was studied. The sensitivity of an immunoglobulin G test was 99 to 100% for all age groups, but the specificity declined by age group, from 99% for those aged 15 to 49 years to 75% for those aged ≥65 years. The exclusion of false-positive results for patients with atrophic gastritis improved the specificity to 93 to 97% for the older age groups.

The Effect of Collagen Mediated Platelet Release on Plasma Prekallikrein Activation

1984 ◽  
Vol 51 (01) ◽  
pp. 037-041 ◽  
Author(s):  
K M Weerasinghe ◽  
M F Scully ◽  
V V Kakkar
Keyword(s):  
Platelet Aggregation ◽  
Healthy Volunteers ◽  
Platelet Rich Plasma ◽  
Age Groups ◽  
Inhibitory Effect ◽  
Age Group ◽  
Platelet Factor ◽  
Activated Platelets ◽  
Platelet Release ◽  
Collagen Treatment

SummaryCollagen mediated platelet aggregation caused -5.6 ± 6.7% inhibition and +39.1 ± 15.2% potentiation of prekallikrein activation in plasma from normal healthy volunteers between 20–40 and 50–65 years of age, respectively (n = 15, p <0.01). The amouns of platelet factor-four (PF4) released in the two groups were not significantly different. Collagen treatment in the presence of indomethacin caused +11.5 ± 3.6% and +59.6 ± 19.5% potentiation in the 20–40 and 50–65 age groups respectively (p <0.02). Adrenaline mediated platelet aggregation caused -55.2 ± 7.1% and -35.2 ± 8.3% inhibition in the 20–40 and 50–65 age groups, respectively. Collagen treatment of platelet-deficient-plasma and platelet-rich-plasma in EDTA also caused potentiation of prekallikrein activation.The results indicate that the observed degree of prekallikrein activation after platelet aggregation is a net result of the inhibitory effect of PF4 and the potentiatory effect of activated platelets. The potentiatory effect was greater after collagen treatment as compared to adrenaline treatment, and in the 50–65 age group as compared to the 20–40 age group.

Rapid detection of Rotavirus antigen in stool sample of acute diarrheic children

2012 ◽  
Vol 6 (1) ◽  
pp. 11-13
Author(s):  
Sushmita Roy ◽  
S.M. Shamsuzzaman ◽  
K.Z. Mamun
Keyword(s):  
Stool Sample ◽  
Rapid Detection ◽  
Cross Sectional Study ◽  
Rapid Diagnosis ◽  
Age Group ◽  
College Hospital ◽  
Cross Sectional ◽  
Rotavirus Infection ◽  
Medical College ◽  
Stool Samples

Rotavirus is one of the leading causes of pediatric diarrhea globally. Accurate and rapid diagnosis of Rotavirus diarrhea should reduce unnecessary use of antibiotics and ultimately reduce drug resistance. Study was designed for rapid diagnosis of Rotavirus antigen in stool sample by ICT (Immunochromatographic test) as well as to observe the seasonal variation of rotavirus infection. This cross sectional study was carried out in the department of Microbiology, Dhaka Medical College from January 2011 to December 2011. Eighty stool samples were collected from Dhaka Shishu Hospital and Dhaka Medical College Hospital. All samples were tested for rotavirus antigen by ICT. Among 80 patients, 42 (52.5%) samples were positive for rotavirus antigen. Among these 42 positive samples, 30 (71.43%) were from 0-12 months of age group, 10 (23.81%) from 13 to 24 months of age group and rest 2 (4.76%) from 25 to 36 months of age group. Rotavirus Ag was detected in stool samples from January to April and another peak episode from October to December. Considering the importance of Rotavirus associated diarrhea, rapid detection of Rotavirus infection in human is substantially needed and should be routinely practiced.DOI: http://dx.doi.org/10.3329/bjmm.v6i1.19354 Bangladesh J Med Microbiol 2012; 06(01): 11-13

Health and Nutritional Status of Orphan Children’s Living in Orphanages with Special Reference to District Anantnag of Jammu and Kashmir

2016 ◽  
Vol 3 (2) ◽  
Author(s):  
Mashkoor Ahmad Lone ◽  
Dr. P. Ganesan
Keyword(s):  
Age Groups ◽  
Indian Academy ◽  
Dental Enamel ◽  
Nutritional Intake ◽  
Age Group ◽  
Asian Countries ◽  
Jammu And Kashmir ◽  
Material Resources ◽  
Height For Age ◽  
Weight For Age

The practice of placing deprived children having least or no emotional and material resources, in orphanages has since long been prevailing in socio -economically poor Asian countries. A sample of 30 children residing in orphanage in district Anantnag in the age group of 13-18 years was selected for the present study. Most of the children were found socially and psychologically disturbed. As per Indian Academy Paediatrics (IAP) classification with respect to weight for age the condition was not bad that as approximately 67% percent of the children were found to be normal. In the same way height for age as per Waterloo’s classification shown that more than half of the children were normal. On clinical examination approximately 47% of children were normal, while as rest were suffering from dispigmentation of hair, moon face, xerosis of skin cheilosis, magenta tongue, spongy bleeding gums, oedema, conjuctival xerosis, and mottled dental enamel. The findings indicated that nutritional intake was deficient for all nutrients when compared to, Recommended Daily Allowances Chart (RDA) for all age groups which may be linked to poor planning of menus in orphanages.

An Epidemiological, Diagnostic and Therapeutic Study of Giardia lamblia in Anbar Province – Iraq

2019 ◽  
Vol 9 (01) ◽  
pp. 39-45
Author(s):  
Suad Shallal Shahatha
Keyword(s):  
Teaching Hospital ◽  
Rural Areas ◽  
Giardia Lamblia ◽  
Urban Areas ◽  
Culture Media ◽  
Age Groups ◽  
Stool Samples ◽  
Women And Children ◽  
Teucrium Polium L ◽  
Therapeutic Study

This study was carried out to investigate the epidemiology of Giardia lamblia parasites in patients who visited some of the hospitals in Anbar province, which included (Fallujah Teaching Hospital, Ramadi Teaching Hospital, Ramadi Teaching Hospital for Women and Children and Hit Hospital) during by examining 864 stool samples in a direct examination method, The results revealed the infection rate was 41.7 % and the percentage of infection among males 47.8% is higher than that of females 35.4% with significant differences (p≤0.05). The age groups (1-9) years recorded the highest rates 55.4% and the lowest rate 13.6% in the age group (40-49) years. The highest rate of infection was 62.5% during the month of June, while the month of October was the lowest rate 5% and significant differences. The incidence rate in rural areas was 50.6% higher than in the urban areas 32.5%. The study also included the effect of Teucrium polium L. on the parasite in the culture media HSP-1, the concentrations of 0.5-3 mg / mL significantly affected Giardia, it was noted whenever the greater the concentration, the greater the effect during different treatment periods (1-4) days, as the highest concentration 3 mg/ml killed all Giardia parasites on the fourth day of treatment.

Hepatic Encephalopathy and Liver Failure in Pediatric Patient with hepatitis A

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Shaima’a Dakhel AbdulHassa
Keyword(s):  
Hepatic Encephalopathy ◽  
Liver Failure ◽  
Hepatitis A ◽  
Gene Sequence ◽  
Stool Examination ◽  
Age Group ◽  
Significant Difference ◽  
Medical City ◽  
Sequence Method ◽  
Stool Samples

Gairdia lamblia is one of parasites that cause intestinal problems within the human body, particularly private travelers and children. In this study a total of (100) diarrheal patients, 20 patients with Giardiasis were identified by fecal antigen. 9 out of 20(20%) of them were infected by fecal antigen, while 9(9%) of them were infected by using the screening general stool examination (GSE). The stool samples were collected from patient how vested the Medical City/ Baghdad and Tikrit teaching Hospital during the period from 1 st may 2018 to 1 February 2019. The results revealing a significant difference (p andlt; 0.05) between the two methods of detection for G. lamblia (Fecal antigen method and GSE). IT has been shown that out of 20 infected individuals 12(12%) were males and 8(8%) were females, indicating regarding no significant deference in the distribution of Giardiasis among genders. In regard the age, our results showed that highest infection rate 8(3.2%) was recorded in the age group (10-19) years, followed by the age group (20-2) years which was 692.4%). In this study five mutations were recorded at position (926, 1094, 1202and 1304), by using tpiA gene sequence method, and tpiB gene was on point mutation change (G254A), in the position (85) of triose phosphate isomease.

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