scholarly journals Sequential Pathology of a Genotype XIII Newcastle Disease Virus from Bangladesh in Chickens on Experimental Infection

Pathogens ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 539
Author(s):  
Congriev Kumar Kabiraj ◽  
Tanjin Tamanna Mumu ◽  
Emdadul Haque Chowdhury ◽  
Mohammad Rafiqul Islam ◽  
Mohammed Nooruzzaman
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Bursa Of Fabricius ◽  
Lymphoid Tissues ◽  
Hemorrhagic Pneumonia ◽  
Advanced Stages ◽  
Lymphoid Depletion ◽  
The Brain ◽  
Bangladeshi Strain

The sequential pathology of a genotype XIII Bangladeshi strain of Newcastle disease virus (NDV) was studied in 5-weeks old chickens. Layer chickens of ISA Brown breed were inoculated through the intranasal and intraocular routes with the BD-C161/2010 strain of NDV and examined at different times post-infection (pi). NDV-infected chickens showed depression at 3 days pi (dpi) followed by dropped wings, paralysis and death starting at 4 dpi. Lungs of infected chickens showed hemorrhagic lesions starting at 24 hours pi (hpi) that was followed by pallor and slight contraction by 2 to 3 dpi and subsequently developed into severe hemorrhagic pneumonia with mononuclear cell infiltration. Hemorrhagic and necrotizing lesions were found in different visceral organs including proventriculus, intestine, gut-associated lymphoid tissues, liver and kidneys starting at 3 dpi that progressed rapidly. Severe lymphoid depletion was observed in the thymus, spleen and bursa of Fabricius starting at 1–3 dpi followed by hemorrhages, necrosis, inflammation and atrophy at 4–5 dpi. In the brain, mild neuronal lesions such as focal to diffuse encephalitis with encephalomalacia was observed at 2–3 dpi and moderate and diffuse meningoencephalitis with encephalomalacia at advanced stages. In conclusion, the BD-C161/2010 strain of NDV produced lesions typical of velogenic viscerotropic pathotype of NDV.

scholarly journals Indicators of the molecular pathogenesis of virulent Newcastle Disease Virus in chickens revealed by transcriptomic profiling of spleen

2020 ◽  
Author(s):  
Mohammad Rabiei ◽  
Wai Yee Low ◽  
Milton McAllister ◽  
Yan Ren ◽  
Mohamad Cahyono ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Molecular Pathogenesis ◽  
Bursa Of Fabricius ◽  
Control Group ◽  
Rna Seq ◽  
Virulent Newcastle Disease Virus ◽  
Rho Family Gtpases ◽  
Lymphoid Depletion

Abstract Newcastle disease virus (NDV) has caused significant outbreaks in South-East Asia, particularly in Indonesia in recent years. Recently emerged genotype VII NDVs (NDV-GVII) have shifted their tropism from gastrointestinal/respiratory tropism to a lymphotropic virus, invading lymphoid organs including spleen and bursa of Fabricius to cause profound lymphoid depletion. In this study, we aimed to identify candidate genes and biological pathways that contribute to the disease caused by this neurotropic velogenic NDV-GVII. A transcriptomic analysis based on RNA-Seq of spleen was performed in chickens challenged with NDV-GVII and a control group. In total, 6361 genes were differentially expressed that included 3506 up-regulated genes and 2855 down-regulated genes. Real-Time PCR of ten selected genes validated the RNA-Seq results as the correlation between them is 0.98. Functional and network analysis of DEGs showed altered regulation of ElF2 signalling, mTOR signalling, proliferation of lymphatic system cells, signalling by Rho family GTPases and synaptogenesis signalling in spleen. We have also identified modified expression of IFIT5, PI3K, AGT and PLP1 genes in NDV-GVII infected chickens. Our findings in activation of autophagy-mediated cell death, lymphotropic and synaptogenesis signalling pathways provide new insights into the molecular pathogenesis of this newly emerged NDV-GVII.

scholarly journals Indicators of the molecular pathogenesis of virulent Newcastle disease virus in chickens revealed by transcriptomic profiling of spleen

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mohammad Rabiei ◽  
Wai Yee Low ◽  
Yan Ren ◽  
Mohamad Indro Cahyono ◽  
Phuong Thi Kim Doan ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Molecular Pathogenesis ◽  
Differentially Expressed ◽  
Bursa Of Fabricius ◽  
Control Group ◽  
Rna Seq ◽  
Virulent Newcastle Disease Virus ◽  
Lymphoid Depletion

AbstractNewcastle disease virus (NDV) has caused significant outbreaks in South-East Asia, particularly in Indonesia in recent years. Recently emerged genotype VII NDVs (NDV-GVII) have shifted their tropism from gastrointestinal/respiratory tropism to a lymphotropic virus, invading lymphoid organs including spleen and bursa of Fabricius to cause profound lymphoid depletion. In this study, we aimed to identify candidate genes and biological pathways that contribute to the disease caused by this velogenic NDV-GVII. A transcriptomic analysis based on RNA-Seq of spleen was performed in chickens challenged with NDV-GVII and a control group. In total, 6361 genes were differentially expressed that included 3506 up-regulated genes and 2855 down-regulated genes. Real-Time PCR of ten selected genes validated the RNA-Seq results as the correlation between them is 0.98. Functional and network analysis of Differentially Expressed Genes (DEGs) showed altered regulation of ElF2 signalling, mTOR signalling, proliferation of cells of the lymphoid system, signalling by Rho family GTPases and synaptogenesis signalling in spleen. We have also identified modified expression of IFIT5, PI3K, AGT and PLP1 genes in NDV-GVII infected chickens. Our findings in activation of autophagy-mediated cell death, lymphotropic and synaptogenesis signalling pathways provide new insights into the molecular pathogenesis of this newly emerged NDV-GVII.

Invasion of the Brain of Chicken by Newcastle Disease Virus

1966 ◽  
Vol 10 (1) ◽  
pp. 122
Author(s):  
S. Vadlamudi ◽  
R. P. Hanson
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
The Brain

scholarly journals Molecular and pathological characterisation of genotype VII Newcastle disease virus on Egyptian chicken farms during 2016–2018

2020 ◽  
Vol 68 (2) ◽  
pp. 221-230
Author(s):  
Mohamed R. Mousa ◽  
Faten F. Mohammed ◽  
Ayman H. El-deeb ◽  
Hanan Saad Khalefa ◽  
Kawkab A. Ahmed
Keyword(s):  
Phylogenetic Analysis ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Viral Antigen ◽  
The Novel ◽  
Necrotising Pancreatitis ◽  
Lymphoid Depletion ◽  
Syncytia Formation ◽  
Genotype Vii

AbstractNewcastle disease virus (NDV) remains a constant threat to the poultry industry even with intensive vaccination programmes. In the present study, 40 samples were collected from farms showing high mortalities in some Egyptian governorates between 2016 and 2018. Tracheal samples were collected for virus isolation and confirmed by real-time RT-PCR. Molecular characterisation was performed by sequencing, followed by phylogenetic analysis of the novel sequences. Histopathological and immunohistochemical examinations were performed on different organs from NDV-infected broilers. The phylogenetic analysis revealed that the NDV isolates from different areas of Egypt were genetically closely related and all belonged to genotype VII. The histopathological hallmarks included haemorrhagic tracheitis, interstitial pneumonia with syncytia formation, haemorrhagic proventriculitis, necrotising pancreatitis, pan-lymphoid depletion, non-suppurative encephalitis and nephritis. Immunological detection of NDV antigen clarified the widespread presence of viral antigen in different organs with severe lesions. The present study confirmed that a virulent NDV of genotype VII became the predominant strain, causing severe outbreaks in poultry farms in Egypt. The presence of viral antigen in different organs indicates the pantropic nature of the virus. Immunohistochemistry was a very useful diagnostic tool for the detection of NDV antigen.

Insights into the chicken bursa of fabricius response to Newcastle disease virus at 48 and 72 hours post-infection through RNA-seq

2019 ◽  
Vol 236 ◽  
pp. 108389 ◽  
Author(s):  
Xiangwei Wang ◽  
Yanqing Jia ◽  
Juan Ren ◽  
Haijin Liu ◽  
FathalrhmanEisa Addoma Adam ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Post Infection ◽  
Bursa Of Fabricius ◽  
Rna Seq

Pathogenesis of Newcastle Disease Virus Genotype VII in Chickens Vaccinated with LaSota and Inactivated Newcastle Disease Vaccines

2020 ◽  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Clinical Signs ◽  
Bursa Of Fabricius ◽  
Virus Genotype ◽  
Challenge Virus ◽  
Significant Difference ◽  
Group D ◽  
Genotype Vii

Newcastle disease (ND) is one of the most serious viral diseases affecting poultry farms in different countries. Many outbreaks -even in vaccinated poultry flocks- were recorded in the last few years caused by Newcastle disease virus (NDV) genotype VII. This study was conducted to compare the pathogenesis of NDV genotype VII in non-vaccinated chickens and chickens vaccinated with NDV genotype II live (LaSota) and inactivated vaccines. One hundred 1-day-old chicks were divided into four equal groups; 25 for each. Groups A and B were kept unvaccinated. Group C was vaccinated with LaSota, and group D was vaccinated with both LaSota and inactivated NDV vaccine. Group A was kept as nonchallenged control blank group, while groups B, C and D were challenged intranasally by 0.1 ml 106 EID50 NDV genotype VII at 25-day of age. Three chickens were sacrificed from each group at 2, 5- and 10-days post challenge (dpc). Tissue specimens from trachea, lungs, bursa of fabricius, spleen and thymus were collected for histopathology and immunohistochemistry. NDV genotype VII challenge virus did not induce mortality in both vaccinated groups. Both vaccination programs resulted also in less severe clinical signs and histopathological lesions comparing to non-vaccinated challenged birds. Tracheal lesion score was significantly low in group D at 10 dpc while no significant difference was recorded between groups C and D in lungs. All lymphoid organs showed significantly less severe pathological alterations and depletion in groups C and D comparing to group B. Our results indicated that mis-matched genotype NDV vaccines could alleviate the pathological effect of the NDV challenge virus but do not provide complete protection of the infected host organs.

Sign in / Sign up

Export Citation Format

Share Document