scholarly journals Efflux MexAB-Mediated Resistance in P. aeruginosa Isolated from Patients with Healthcare Associated Infections

Pathogens ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 471
Author(s):  
Rania M. Kishk ◽  
Mohamed O. Abdalla ◽  
Abdullah A. Hashish ◽  
Nader A. Nemr ◽  
Nihal El Nahhas ◽  
...  
Keyword(s):  
Antimicrobial Agents ◽  
Efflux Pump ◽  
Suez Canal ◽  
University Hospital ◽  
Resistant Organism ◽  
Healthcare Associated Infections ◽  
University Hospitals ◽  
Binding Interactions ◽  
Adequate Treatment ◽  
Healthcare Associated

Today, one of the most important challenges for physicians is the adequate treatment of infections due to multidrug resistant organism (MDR). Pseudomonas aeruginosa is considered an opportunistic organism causing different types of healthcare associated infections (HAIs). We aimed to investigate the MDR and pandrug resistance (PDR) rate in P. aeruginosa in our region and detect efflux-pump mexAB genes and the proposed binding interactions of five different categories of antimicrobial agents with the mexB pump. A total of 180 non-duplicated P. aeruginosa strains were isolated from patients with HAIs in the Suez Canal University Hospital. Phenotypically, minimum inhibitory concentration (MIC) was done for all MDR and PDR strains before and after addition of efflux pump inhibitor carbonyl cyanide m-chlorophenyl hydrazone (CCCP). Molecular detection of mexA and mexB genes was done by using polymerase chain reaction (PCR). Most of the isolated strains (126 strains) were MDR (70%); only 10 samples (5.5%) were PDR. MexA and mexB genes were detected in 88.2% (120 strains) and 70.5% (96 strains) of stains, respectively. All PDR strains (10 stains) carried both mexA and mexB genes. Efflux mexAB genes were detected in all MDR and PDR strains (136 strains). Molecular modeling studies were performed to investigate the modes of intermolecular binding interactions between the antimicrobial agents and mexB key amino acids that resulted in MDR and PDR. The current study reported high prevalence of MDR and PDR P. aeruginosa in patients with HAIs in the Suez Canal University Hospitals.

Point prevalence of healthcare-associated infections and antibiotic use in a tertiary care teaching hospital in Nepal: A cross-sectional study

2021 ◽  
pp. 175717742110358
Author(s):  
Sailesh Kumar Shrestha ◽  
Swarup Shrestha ◽  
Sisham Ingnam
Keyword(s):  
Prevention And Control ◽  
Tertiary Care ◽  
Antibiotic Use ◽  
Point Prevalence ◽  
University Hospital ◽  
Infection Prevention And Control ◽  
Healthcare Associated Infections ◽  
Prevalence Survey ◽  
Healthcare Associated ◽  
And Control

Information on the burden of healthcare-associated infections (HAIs) and patterns of antibiotic use are prerequisites for infection prevention and control (IPC) and antibiotics stewardship programmes. However, a few studies have been reported from resource-limited settings and many of them have not used standard definitions to diagnose HAI precluding benchmarking with regional or international data. This study aims to estimate the prevalence of HAIs and antibiotic use in our centre. We conducted a point prevalence survey in a 350-bed university hospital in Kathmandu, Nepal in April 2019. We reviewed all patients aged ⩾ 18 years admitted to the hospital for at least two calendar days and evaluated for the three common HAIs—pneumonia, urinary tract infection and surgical site infection. We used the clinical criteria by the European Center for Disease Prevention and Control to diagnose the HAIs. We also collected information on the antibiotics used. Of 160 eligible patients, 18 (11.25%) had HAIs and 114 (87.5%) were on antibiotics, with more than half of them (61/114 patients, 53.5%) receiving two or more antibiotics. This highlights the need for effective implementation of IPC as well as antibiotics stewardship programmes in our centre.

scholarly journals Healthcare-associated infections: bacteriological characterization of the hospital surfaces in the University Hospital of Abomey-Calavi/so-ava in South Benin (West Africa)

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
F. Cyr Doscoph Afle ◽  
Alidéhou Jerrold Agbankpe ◽  
Roch Christian Johnson ◽  
Olivia Houngbégnon ◽  
Sègbè Christophe Houssou ◽  
...  
Keyword(s):  
West Africa ◽  
University Hospital ◽  
Healthcare Associated Infections ◽  
Healthcare Associated ◽  
The University

scholarly journals Surveillance of healthcare-associated infections in a Tunisian university hospital

2019 ◽  
Vol 29 (Supplement_4) ◽  
Author(s):  
H Hannachi ◽  
A Ben Cheikh ◽  
S Bhiri ◽  
H Ghali ◽  
S Khefacha ◽  
...  
Keyword(s):  
Risk Factors ◽  
Intensive Care Unit ◽  
Intensive Care ◽  
University Hospital ◽  
Healthcare Associated Infections ◽  
Drug Resistant ◽  
Healthcare Associated Infection ◽  
Carbapenem Resistant ◽  
Independent Risk Factors ◽  
Healthcare Associated

Abstract Introduction Healthcare -associated infections has become a worldwide public health problem. The aim of this study was to estimate the incidence of healthcare- associated infections in a university hospital of Tunisia. Methods This was a cohort study conducted in six intensive care units in a university hospital of Tunisia during three months (from august to October 2018). Data was provided from patients’ files. Data entry and analysis was done using SPSS version 22. Multivariate analysis was used in order to identify independent risk factors for healthcare associated infection. Results A total of 202 patients were enrolled in this study. The incidence rate of healthcare-associated infections was 53,96%(109/202). The ratio infection/infected was estimated to 1.65(109/66). The incidence of multi-drug resistant pathogens was 21,28% (43/202). The most common resistant pathogens included pseudomonas aeruginosa resistant to cefdazidime in 13,76%(15/109) followed by those resistant to extended spectrum cephalosporin 11.92% (13/109), followed by carbapenem-resistant acinetobcater baumanii 6,42%(7/109) then by carbapenem resistant pathogens and enterococcus resistant to vancomycin 2.75%(3/109) and finally staphylococcus aureus resistant to methicillin 2.1%(2/1.83). The multivariate analysis showed that long duration of central line catheterisation (RR = 7.44; 95%CI[2.79-19.82]), tracheotomy(RR = 8.61;95%CI[2.09-35,39]) and length of stay (RR = 1.08; 95%CI[1.04-1.13]) were found as independent risk factors for healthcare -associated infection. Conclusions The emergence of mutli-drug resistant pathogens needs to be deeply studied and effective measures have to be taken in order to detect and prevent transmission of resistant strains and/or their resistance determinants, especially those with phenotypes having the fewest viable treatment options. Key messages The incidence of healthcare associated infection in the intensive care unit was high. Effective measures have to be taken in the intensive care unit to detect and prevent transmission of resistant pathogens.

scholarly journals Are Invasive Procedures and a Longer Hospital Stay Increasing the Risk of Healthcare-Associated Infections among the Admitted Patients at Hiwot Fana Specialized University Hospital, Eastern Ethiopia?

2020 ◽  
Vol 2020 ◽  
pp. 1-7 ◽  
Author(s):  
Moti Tolera ◽  
Dadi Marami ◽  
Degu Abate ◽  
Merga Dheresa
Keyword(s):  
Cigarette Smoking ◽  
Hospital Stay ◽  
University Hospital ◽  
Infection Prevention And Control ◽  
Healthcare Associated Infections ◽  
Invasive Procedures ◽  
Healthcare Associated Infection ◽  
Eastern Ethiopia ◽  
Healthcare Associated ◽  
The Impact

Background. Healthcare-associated infection is a major public health problem, in terms of mortality, morbidity, and costs. Majorities of the cause of these infections were preventable. Understanding the potential risk factors is important to reduce the impact of these avoidable infections. The study was aimed to identify factors associated with healthcare-associated infections among patients admitted at Hiwot Fana Specialized University Hospital, Harar, Eastern Ethiopia. Methods. A cross-sectional study was carried out among 433 patients over a period of five months at Hiwot Fana Specialized University Hospital. Sociodemographic and clinical data were obtained from a patient admitted for 48 hours and above in the four wards (surgical, medical, obstetrics/gynecology, and pediatrics) using a structured questionnaire. A multivariate logistic regression model was applied to identify predictors of healthcare-associated infections. A p value <0.05 was considered statistically significant. Results. Fifty-four (13.7%) patients had a history of a previous admission. The median length of hospital stay was 6.1 days. Forty-six (11.7%) participants reported comorbid conditions. Ninety-six (24.4%) participants underwent surgical procedures. The overall prevalence of healthcare-associated infection was 29 (7.4%, 95% CI: 5.2–10.6). Cigarette smoking (AOR: 5.18, 95% CI: 2.15–20.47), staying in the hospital for more than 4 days (AOR: 4.29, 95% CI: 2.31–6.15), and undergoing invasive procedures (AOR: 3.58, 95% CI: 1.11–7.52) increase the odds of acquiring healthcare-associated infections. Conclusion. The cumulative prevalence of healthcare-associated infections in this study was comparable with similar studies conducted in developing countries. Cigarette smoking, staying in the hospital for more than 4 days, and undergoing invasive procedures increase the odds of healthcare-associated infections. These factors should be considered in the infection prevention and control program of the hospital.

Nasal Carriage Rate and Antimicrobial Resistance Profiles of MethicillinResistant Staphylococcus Aureus (MRSA) among Health Care Workers in Tanta University Hospital

2020 ◽  
Vol EJMM29 (4) ◽  
pp. 1-7
Author(s):  
Marwa S. Taha ◽  
Eman A. Younis ◽  
Eman E. Hegazy
Keyword(s):  
Infection Control ◽  
Nasal Carriage ◽  
University Hospital ◽  
Resistant Organism ◽  
University Hospitals ◽  
Carriage Rate ◽  
Mannitol Salt Agar ◽  
Vulnerable Patients ◽  
Nasal Swabs ◽  
History Of

Background: MRSA is the most commonly known antimicrobial-resistant organism in hospitals worldwide. Objectives: This study aimed to detect the prevalence of MRSA carriage and its antibiogram among HCWs in Tanta University hospitals to improve infection control and preventive measures. Methodology: 223 nasal swabs from HCWs were inoculated onto Mannitol salt agar. Detection of MRSA was performed phenotypically using cefoxitin disc diffusion test on Muller–Hinton agar plates. Confirmation of MRSA was done by determining minimum inhibitory concentration (MIC) of oxacillin by using E Test Strips. Results: Amongst the HCWs, 88 doctors and 135 nurses were randomly selected. The overall frequency of S. aureus nasal carriage was 129/223. Of the 129 S. aureus isolates, (17%) were MRSA. Internal medicine had a high proportion of MRSA positive (36.4%). (63.6%) of the MRSA positive HCWs had a history of using antibiotics during the past 3 months. A high frequency (77.3%) of MRSA was detected among nurses. (50%) HCWs with 5:10 years of working experience were colonized with MRSA. Conclusion: Multi-drug resistant organisms such as MRSA are a major public health challenge. Colonized HCWs are asymptomatic carriers and can transmit MRSA to vulnerable patients. To control the transmission of MRSA in hospitals, multidisciplinary efforts are recommended to implement and improve infection control policies.

scholarly journals Use of personal digital assistants to detect healthcare-associated infections in a neonatal intensive care unit in Egypt

2016 ◽  
Vol 10 (11) ◽  
pp. 1250-1257 ◽  
Author(s):  
Elham AM El-Feky ◽  
Doa’a A Saleh ◽  
Jehan El-Kholy ◽  
Ahmed Mahmoud Sayed ◽  
Yasmeen Mansi ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Neonatal Intensive Care Unit ◽  
Neonatal Intensive Care ◽  
University Hospital ◽  
Personal Digital Assistants ◽  
Healthcare Associated Infections ◽  
Coagulase Negative Staphylococci ◽  
Promising Alternative ◽  
Healthcare Associated

Introduction: Personal digital assistants (PDAs) used in electronic laboratory-based surveillance are a promising alternative to conventional surveillance to detect healthcare-associated infections (HAIs). The aim of the study was to monitor, detect, and analyze HAIs using PDAs in a neonatal intensive care unit (NICU). Methodology: In this descriptive study, 1,053 neonates admitted to the NICU in the obstetrics and gynecology ward at the Cairo University hospital were included and evaluated for HAIs by collecting data using PDAs programmed by Naval Medical Research Unit 3, Cairo, with the definitions for HAIs provided by the National Healthcare Safety Network of the Centers for Disease Control and Prevention. Case records were reviewed three times a week over 19 months, from March 2012 to September 2013. Results: Of 124 suspected episodes of infection recorded in PDAs, 89 confirmed episodes of infection were identified. HAI and NICU infection rates were 7.4 and 2.72/1,000 patient-days, respectively. Primary bloodstream infection was detected in 81 episodes and pneumonia in 8 episodes. The majority of infections (62%) were acquired in the ward before NICU admission. Klebsiella spp. was isolated most frequently (42%), followed by coagulase-negative Staphylococci (31%). Conclusions: This study is the first to report the use of PDAs in surveillance to detect HAIs in the NICU in our hospital. The majority of infections were acquired at the obstetric care department, indicating the importance of implementing rigorous prevention and control programs and a more detailed surveillance to identify other risk factors for infections.

scholarly journals Emergence of MRSA infections in horses in a veterinary hospital: strain characterisation and comparison with MRSA from humans

2006 ◽  
Vol 11 (1) ◽  
pp. 13-14 ◽  
Author(s):  
C Cuny ◽  
J Kuemmerle ◽  
C Stanek ◽  
B Willey ◽  
B Strommenger ◽  
...  
Keyword(s):  
Methicillin Resistant Staphylococcus Aureus ◽  
Companion Animals ◽  
Public Health Problem ◽  
University Hospital ◽  
Healthcare Associated Infections ◽  
Central European ◽  
Healthcare Associated ◽  
Veterinary Hospital ◽  
European University ◽  
Strain Characterisation

Methicillin-resistant Staphylococcus aureus has become an emerging public health problem worldwide, no longer only associated with healthcare-associated infections. With the exception of some recent reports concerning infections in cats, dogs and horses, infections with MRSA in companion animals have been infrequently reported. Here we submit findings for MRSA infections in horses in a central European university hospital.

scholarly journals Healthcare-associated infections in a tunisian university hospital: from analysis to action

2015 ◽  
Vol 20 ◽  
Author(s):  
Mohamed Mahjoub ◽  
Nebiha Bouafia ◽  
Waadia Bannour ◽  
Tasnim Masmoudi ◽  
Rym Bouriga ◽  
...  
Keyword(s):  
University Hospital ◽  
Healthcare Associated Infections ◽  
Healthcare Associated
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