scholarly journals Human Papillomavirus E6/E7 Expression in Preeclampsia-Affected Placentae

Pathogens ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 239
Author(s):  
Ashley L. Reily-Bell ◽  
Amanda Fisher ◽  
Bryony Harrison ◽  
Sara Bowie ◽  
Sankalita Ray ◽  
...  
Keyword(s):  
High Risk ◽  
Pregnancy Complications ◽  
First Trimester ◽  
Placental Pathology ◽  
Hpv Dna ◽  
Hpv Types ◽  
E6 And E7 ◽  
High Risk Hpv ◽  
Geographical Locations ◽  
Hpv 18

Whether HPV is causative of pregnancy complications is uncertain. E6 and E7 affect functions underling preeclampsia (PET) in cultured trophoblasts, but whether E6 and E7 is produced in the placenta is uncertain. Here, we investigated whether E6/E7 was expressed in the placentae from pregnancies with PET, other pregnancy complications (fetal growth restriction (FGR) and diabetes mellitus), and uncomplicated pregnancies. Placental tissues collected from two geographical locations were subjected to RNAscope analyses of high- and low- risk E6/E7, and individual HPV types identified using an HPV array. High-risk E6/E7 expression was increased in both PET cohorts, (81% and 86% of patients positive for high-risk HPV DNA compared to 13% of control patients). Various HPV types were identified. Although HPV 18 was the most frequent in all cohorts, the majority of individuals had multiple HPV types (55% of the PET compared to 25% of the control cohort). Further evidence that E6 and E7 is present early when placental pathology underlying preeclampsia is established, is provided with the finding of high-risk E6/E7 in the first-trimester placenta anchoring trophoblast. In conclusion, E6/E7 expression and multiple HPV types were frequent in placentae from preeclampsia-complicated pregnancies.

Prevalence and Distribution of High-Risk Human Papillomavirus Genotypes in Invasive Carcinoma of the Uterine Cervix in Uruguay

2013 ◽  
Vol 23 (3) ◽  
pp. 527-532 ◽  
Author(s):  
Nora Berois ◽  
Patricia De Cremoux ◽  
Daniel Mazal ◽  
Adela Sica ◽  
Mabel Cedeira ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Dna Sequences ◽  
Invasive Cervical Cancer ◽  
Human Papillomaviruses ◽  
Geographical Differences ◽  
Hpv Dna ◽  
High Prevalence ◽  
Hpv Types ◽  
High Risk Hpv

ObjectivesPersistent infection with specific genotypes of human papillomaviruses (HPVs) is the main cause of invasive cervical cancer (ICC). Only a few of the various HPV types account for most of the cases worldwide, and geographical differences in their distribution are evident. Data from locally prevalent genotypes are essential in view of introduction of HPV type-specific prophylactic vaccines.MethodsIn this work, we have investigated HPV type distribution in samples of ICC cases that occurred in Uruguayan women. DNA extracted from ICC treated in Centro Hospitalario Pereira Rossell of Montevideo between 1999 and 2007 were analyzed. Search and typing were performed by polymerase chain reaction using generic GP5+/GP6+ primers and specific primers for HPV types 16, 18, 33, and 45. Positive GP5+/GP6+ samples, which were negative for all 4 high-risk HPV-specific types screened were further analyzed by sequencing.ResultsHuman papillomavirus DNA sequences were found in 163 (92.6%) of 176 cases. The most prevalent genotypes were HPV16 (67.6%) and HPV18 (8.5%) followed by HPV45 (6.8%) and HPV33 (3.4%), as single or mixed infection. Other less frequent genotypes were HPV31, HPV35, HPV39, HPV51, HPV52, HPV58, HPV66, and HPV73. The viral type could not be determined (HPV X) in 1 case (0.6%) of the HPV DNA–positive cervical cancers and double infections were found in 1.7% of the cases. The higher percentage of most aggressive HPV (16/18/45) genotypes was detected in cases diagnosed at younger than 60 years old, whereas these genotypes were less frequent in older patients.ConclusionWe conclude that HPV types 16, 18, and 45 have a very high prevalence in ICC of Uruguayan women. Results provide evidence that 16 of 18 infections are more aggressive, but most cancers could be vaccine preventable.

High-risk HPV types in Tunisia. A pilot study reveals an unexpectedly high prevalence of types 58 and 82 and lack of HPV 18 among female prostitutes

2006 ◽  
Vol 78 (7) ◽  
pp. 950-953 ◽  
Author(s):  
Federico De Marco ◽  
Fatma Houissa-Kchouk ◽  
Ridha Khelifa ◽  
Maria Luisa Marcante
Keyword(s):  
Pilot Study ◽  
High Risk ◽  
High Prevalence ◽  
Female Prostitutes ◽  
Hpv Types ◽  
High Risk Hpv ◽  
Hpv 18

scholarly journals HPV Testing of Biobanked Liquid-Based Cytology – a Validation Study

2016 ◽  
Vol 31 (2) ◽  
pp. 218-223 ◽  
Author(s):  
Gabriella Lillsunde Larsson ◽  
Mats G. Karlsson ◽  
Gisela Helenius
Keyword(s):  
High Risk ◽  
Dna Analysis ◽  
Hpv Testing ◽  
Hpv Dna ◽  
Significant Difference ◽  
Before And After ◽  
Valid Test ◽  
Hpv Types ◽  
Liquid Based Cytology ◽  
E6 And E7

Introduction The aim of the study was to investigate whether biobanked liquid-based cytology (LBC) vaginal samples could be reanalyzed for the biomarkers HPV DNA and mRNA without loss of sensitivity. Methods One hundred LBC samples with ASCUS or CIN1 were tested for HPV DNA and mRNA before and after biobanking. DNA analysis targeted the viral genes E6 and E7, 12 high-risk and 2 low-risk HPV types together with the human control gene HBB, using real-time PCR. The Aptima HPV assay was used for mRNA analysis of 14 high-risk HPV types. Results With Aptima there was 84% agreement between results before and after biobanking. The sensitivity and specificity were 0.79 (95% CI, 0.68-0.88) and 0.94 (95% CI, 0.80-0.99), respectively. With the DNA-based method, the agreement between results was 87%, the sensitivity 0.85 (95% CI, 0.75-0.92) and the specificity 0.95 (95% CI, 0.77-1.00). Both methods presented a significant difference between positive results before and after biobanking; McNemar test: p = 0.004, p = 0.003, Cohen's kappa: 0.67 (95% CI, 0.53-0.81), 0.68 (95% CI, 0.52-0.84). Cycle threshold values for the DNA method were higher for all genotypes after biobanking, except for HPV-59. Some loss of sensitivity was seen after biobanking but the concordance between HPV detection before and after biobanking was good for both evaluated methods. Conclusions Biobanking of LBC vaginal samples offers a good platform for HPV testing and could be extended to further molecular analyses. However, in order to ensure a valid test result a larger portion needs to be analyzed from the biobanked sample.

scholarly journals Expression of the high-risk human papillomavirus type 18 and 45 E7 oncoproteins in cervical carcinoma biopsies

2005 ◽  
Vol 86 (12) ◽  
pp. 3235-3241 ◽  
Author(s):  
Marc Fiedler ◽  
Sigrun Ressler ◽  
Beatriz Campo-Fernández ◽  
Andreas Laich ◽  
Lars Jansen ◽  
...  
Keyword(s):  
High Risk ◽  
Cervical Carcinoma ◽  
Human Papillomaviruses ◽  
Hpv 16 ◽  
Cervical Cancers ◽  
Hpv Types ◽  
High Level ◽  
E7 Proteins ◽  
High Risk Hpv ◽  
Hpv 18

E7 proteins are major oncoproteins of high-risk human papillomaviruses (HPVs), which play a key role in cervical carcinogenesis. These proteins have been shown to immortalize primary human cells. Due to the absence of antibodies with suitable sensitivity and specificity, little is known about expression of the E7 oncoproteins in naturally infected tissues. Recently, high-level expression of the E7 protein of HPV-16, the most prevalent oncogenic HPV type, was demonstrated in cervical carcinomas by immunohistochemistry; however, approximately 15 additional high-risk HPV types are known to be associated with cervical carcinoma. It is unknown whether the E7 oncoproteins of HPV-18 and -45, the second and third most prevalent HPV types, are expressed in cervical cancers. Using antibodies against HPV-18 and -45 E7 proteins, it is shown here for the first time that the HPV-18 and -45 E7 proteins can be detected in cervical carcinoma biopsies. Together with anti-HPV-16 E7 antibodies, this could create the possibility of detecting E7 oncoproteins in approximately 80 % of all cervical cancers.

scholarly journals Results of the first human papilloma virus center in Hungary (2007–2011)

2011 ◽  
Vol 152 (45) ◽  
pp. 1804-1807
Author(s):  
Ádám Galamb ◽  
Attila Pajor ◽  
Zoltán Langmár ◽  
Gábor Sobel
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Human Papilloma Virus ◽  
Hpv Testing ◽  
Papilloma Virus ◽  
Cytological Atypia ◽  
Hpv Dna ◽  
International Data ◽  
Hpv Types ◽  
High Risk Hpv

Human papilloma virus (HPV) is the most common sexually transmitted infection in the 21st century. It has been established that infections with specific HPV types are contributing factors to cervical cancer. Approximately 99.7% of cervical cancers are associated with high risk HPV types. HPV testing plays an important role in the prevention, by decreasing the prevalence and the mortality of cervical cancer. There are 16 HPV-centers operating in Hungary, in which patients undergo HPV screening, cervical exams, and treatment based on standardized guidelines. Patients and methods: The first HPV-center was founded in 2007 in Budapest, at the 2nd Department of Obstetrics and Gynecology, Semmelweis University. This study aimed to define the presence and prevalence of HPV-DNA in the cervical swab samples obtained from patients in our center. Authors conducted to assess the age-specific-prevalence, and HPV type distribution, the associated cervical abnormalities, comparing our results with international data. Results: Overall 1155 woman underwent HPV-testing and genotyping, using polymerase chain reaction. Overall, 55.5% of patients had positive test for HPV DNA types, in which 38.5% for high-risk HPV DNA. Overall prevalence was the highest among females aged 15 to 25years (62.9%). The most common HPV type found was the high risk type 16 (19.5% among the patients with positive HPV testing). Presence of high risk HPV with concurrent cervical cytological abnormality was in 32%. More than two-thirds of woman with cytological atypia (70.6%) were infected with two or more high risk HPV types. HPV 16 was detected in 32% of patients with cytological abnormalities. Conclusions: The results suggest that the prevalence of HPV in this study population exceeds the international data. The results attracts the attention the peak prevalence of the high risk types in the youngest age-group, and the higher risk of cervical abnormality in case of presence of two or more HPV types. The dominance of type 16 and 18 was predictable, but the strong attendance of type 51 and 31 among patients who had cytological atypia, was slightly surprising. Orv. Hetil., 2011, 152, 1804–1807.

scholarly journals Correlation of the ALA-PDT Treatment Efficacy and the HPV Genotype Profile of Genital Warts after Cryotherapy Failure and Podophyllotoxin Therapy in Male Patients

Life ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 146
Author(s):  
Witold Owczarek ◽  
Monika Slowinska ◽  
Irena Walecka ◽  
Magdalena Ciazynska ◽  
Dorota Nowicka ◽  
...  
Keyword(s):  
High Risk ◽  
Treatment Efficacy ◽  
Complete Response ◽  
Genital Warts ◽  
Hpv Infection ◽  
Hpv Dna ◽  
Study Results ◽  
Male Patients ◽  
Hpv Types ◽  
High Risk Hpv

Background: Genital warts are the manifestation of the human papillomavirus (HPV) infection, which may last for weeks or months before the clinical presentation. The primary aim of the study was the correlation of the DNA HPV genotypes eradication with the treatment response in male patients with persistent genital warts. Methods: Twenty-one male patients (age range: 22–58) after failure of cryotherapy and podophyllotoxin treatment were enrolled in the study. Genetic tests (Real Time - PCR method) analyzed the presence of DNA-HPV before and 6 months after four sessions (4 weeks apart) of photodynamic therapy with 5-aminolaevulinic acid (ALA-PDT). The treatment efficacy was evaluated before each PDT session and at the end of the study. Results: The single HPV DNA type was present in 15/21 of the patients (13/15 HPV6). The high-risk HPV types were found in 8/21 subjects, of which 6/8 had several types. Six months after four sessions of PDT, complete response was found in 16/21 (76.19%; p = 0.0007) of patients, and DNA HPV clearance was found in 66.67% (p = 0.03). The eradication rate differed among patients with primary low-risk and high-risk HPV types—76.92% (10/13; p = 0.0003) and 50% (4/8; p = 0.05) respectively. Conclusion: ALA-PDT is an effective treatment even after the failure of previous modalities. The persistence of clinical lesions and high oncological risk HPV types should be an indication for treatment prolongation.

scholarly journals The Distribution and Prevalence of High-Risk HPV Genotypes Other than HPV-16 and HPV-18 among Women Attending Gynecologists’ Offices in Kazakhstan

Biology ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 794
Author(s):  
Gulzhanat Aimagambetova ◽  
Aisha Babi ◽  
Alpamys Issanov ◽  
Sholpan Akhanova ◽  
Natalya Udalova ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Cervical Cancer Prevention ◽  
Multiple Infections ◽  
Hpv 16 ◽  
Hpv Types ◽  
High Risk Hpv ◽  
Hpv 18 ◽  
Great Burden ◽  
Epidemiological Situation

Cervical cancer represents a great burden to public health of women. This study aimed to obtain a nationwide genotyping survey and analysis of high risk-HPV including those that are caused by HPV types other than HPV-16 and HPV-18, among women in Kazakhstan. This study was conducted based on the collection of survey and cervical swabs of 1645 women across the country. The samples were genotyped for high-risk HPV types based on real-time PCR methods. Collected data was analyzed with the focus on high-risk HPV types other than HPV-16 and -18. Infection was present in 22% of women who participated in the study. The most prevalent types were HPV-31 among single infections and HPV-68 among multiple infections. Conclusively, despite the lack of attention high-risk HPV types beyond HPV-16 and -18 get in attempts of cervical cancer prevention in Kazakhstan, their prevalence is high and plays a large role in cervical cancer epidemiological situation.

scholarly journals Molecular Characterization of High-Risk Human Papillomavirus in Women in Bobo-Dioulasso, Burkina Faso

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Ina Marie Angèle Traore ◽  
Théodora Mahoukèdè Zohoncon ◽  
Adama Dembele ◽  
Florencia W. Djigma ◽  
Dorcas Obiri-Yeboah ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
University Hospital ◽  
Cervical Cancers ◽  
High Prevalence ◽  
Bobo Dioulasso ◽  
Hpv Types ◽  
High Risk Hpv ◽  
Hpv 18

High-risk human papillomavirus (HPV) is found in over 99% of cervical cancers. The aim of this study was to determine the prevalence of HPV in a population of women in Bobo-Dioulasso and to identify the high-risk types present in these women. From May to June, 2015, 181 women who came for consultation at the Souro Sanou University Hospital of Bobo-Dioulasso have been included in this study. Uterine endocervical swabs have been taken in these women. DNA obtained by extraction from the samples thus collected was used to determine the prevalence of high-risk human papillomavirus genotypes through real-time PCR. The age of the women ranged from 20 to 56 years with a mean of35.3±8.1years. The prevalence of infection by high-risk HPV types was 25.4% (46/181). The most common high-risk HPV genotypes were HPV 39 (18.5%), HPV 52 (16.7%), HPV 18 (14.8%), and HPV 35 (13.0%). HPV 16 which is included in the HPV vaccines was not found in the population studied. This type of study which is the first one in Bobo-Dioulasso has showed a high prevalence of genotypes HPV 39, HPV 52, and HPV 35 which are not yet covered by a vaccine.

scholarly journals Human Papillomavirus Genotypes and its Prevalence in Normal Population

2016 ◽  
Author(s):  
Tofan W Utami
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Normal Population ◽  
Single Type ◽  
Multiple Infection ◽  
Single Infection ◽  
Hpv Dna ◽  
Hpv Types ◽  
High Risk Hpv ◽  
Type Infection

Objective: Over 200 types of human papillomavirus (HPV) have been recognized on the basis of DNA sequence. Multiple infection is more prone to be persistent than single infection. The purpose of this study is to assess the variation of HPV types and their prevalence among negative VIA as normal population in Indonesian women. Method: We processed cervical swabs from 1,214 patients with negative VIA. HPV DNA and its genotypes were detected using PCR based INNO-Lipa HPV DNA test. We also classified whether each infection is single or multiple. Result: From 1,214 women with negative VIA, 39 (3.21%) samples were positive for HPV DNA. Among them, we detected 19 types of HPV, consisting of 13 types of high-risk HPV, 5 types of low-risk HPV, and 1 type of unknown HPV (type X). The most prevalent type was HPV type 52 (18.31%), followed by type 39 and X with the same proportion (9.86%), and HPV type 16, 18, and 74 (each 8.45%). Of the total 39 HPV-positive samples, 17 (43.6%) showed multiple-type infection and 22 (56.4%) showed single-type infection. The majority of single infection involves high-risk-HPV. The remaining were type 6, 44, 18, 51 and 66, with each single-type infection showing a prevalence of 4.54%. Conclusion: Our study shows that single HPV infection among the negative VIA population are dominated by high-risk type HPV (types 52, 39, 16, and 18). Single infection was more often encountered than multiple infection. [Indones J Obstet Gynecol 2014; 4: 211-215] Keywords: HPV DNA, HPV genotypes, multiple infection, negative VIA, single infection

P–071 Human Papillomavirus Infection and impact on men fertility

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
N Madzunkov ◽  
K Madjunkova
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Male Infertility ◽  
Sperm Quality ◽  
Hpv Infection ◽  
Hpv Dna ◽  
Hpv Types ◽  
Adverse Pregnancy ◽  
High Risk Hpv ◽  
The Impact

Abstract Study question Is there any correlation between men infertility and HPV infection and its impairment on sperm quality? Summary answer There is a significantly higher prevalence of high-risk HPV in infertile men than fertile men. HPV infection does not impair sperm quality. What is known already Many factors may cause the infertility in males and females. Human papillomavirus are the most frequently sexually transmitted DNA viruses and etiological agents of cervical cancers. There is association between HPV infection in females and adverse pregnancy outcomes such as spontaneous abortion and spontaneous preterm delivery. Previous studies have reported the detection of HPV DNA in semen and in different sites of the male reproductive tract, such as glans penis and scrotum. Recent reports suggested that HPV may affect sperm parameters and lead to male infertility. The impact of HPV infection upon male fertility abnormality has received far less attention. Study design, size, duration In this study case control study we examined 38 fertile and 36 men from infertle couples. Participants/materials, setting, methods we examined the swabs of the entire penile surface and semen samples for HPV detection and genotyping from 38 fertile men and 36 from men from infertile couples.HPV were detected with PCR method. Sperm was also examined for its motility, sperm quantity and morphology . Main results and the role of chance Among 36 confirmed infertile males, only 8 (22.22%) cases were tested positive for HPV of semen samples and 2 among fertile men were HPV-positive (5.26%) of semen samples. Among infertile males 14 (38.88%) had HPV positive penile swabs, and only 1 (2,63%) had positive HPV swab among fertile men. The most prevalent HPV types in the male external genitalia were HPV–16. The most prevalent HPV types in semen were HPV–53. This data revealed a significant association between high-risk HPV and male infertility (P = 0.03). Sperm quality (morphology and motility) did not differ significantly between men with seminal HPV infection and uninfected men. Limitations, reasons for caution There were some limitations in the study such as differences in age. sample sizes and the number of HPV genotypes detected. Wider implications of the findings: We need larger studies and more further investigations to confirm the impact of HPV on male infertility. Trial registration number 2

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