scholarly journals Molecular Diagnostics and Pathogenesis of Fungal Pathogens on Bast Fiber Crops

Pathogens ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 223 ◽  
Author(s):  
Yi Cheng ◽  
Xiaoyu Tang ◽  
Chunsheng Gao ◽  
Zhimin Li ◽  
Jia Chen ◽  
...  
Keyword(s):  
Fungal Pathogens ◽  
Elongation Factor ◽  
Cost Effective ◽  
Global Market ◽  
Accurate Diagnosis ◽  
Current Status ◽  
Effective Control ◽  
Translation Elongation ◽  
Bast Fiber ◽  
Control And Prevention

Bast fibers and products derived from them are undergoing a resurgence in demand in the global market. However, fungal diseases have become an important factor limiting their yield and quality, causing devastating consequences for the production of bast fiber crops in many parts of the world. Thus, there is a high demand for effective control and prevention strategies against fungal pathogens. Having rapid, specific, sensitive, and cost-effective tests that can be used for early and accurate diagnosis of disease agents is an essential step of such strategies. The objective of this study was to review the current status of research on molecular diagnosis of fungal pathogens on bast fiber crops. Our search of PubMed identified nearly 20 genera of fungal pathogens on bast fiber crops, among which the five most common genera were Colletotrichum, Pythium, Verticillium, Fusarium, and Golovinomyces. The gene regions that have been used for molecular identifications of these fungi include internal transcribed spacer (ITS), translation elongation factor 1-α (EF-1α), ß-tubulin, calmodulin (CAL), histone subunit 3 (H3), glyceraldehydes-3-phosphate dehydrogenase (GAPDH), etc. We summarize the molecular assays that have been used to identify these fungi and discuss potential areas of future development for fast, specific, and accurate diagnosis of fungal pathogens on bast fiber crops.

scholarly journals Isolation, Molecular Identification and Mycotoxin Profile of Fusarium Species Isolated from Maize Kernels in Iran

Toxins ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 297 ◽  
Author(s):  
Maryam Fallahi ◽  
Hossein Saremi ◽  
Mohammad Javan-Nikkhah ◽  
Stefania Somma ◽  
Miriam Haidukowski ◽  
...  
Keyword(s):  
Fungal Pathogens ◽  
Fusarium Species ◽  
Animal Health ◽  
Elongation Factor ◽  
Ear Rot ◽  
Translation Elongation ◽  
Wide Range ◽  
Severe Reduction ◽  
Maize Kernels ◽  
High Possibility

Fusarium species are among the most important fungal pathogens of maize, where they cause severe reduction of yield and accumulation of a wide range of harmful mycotoxins in the kernels. In order to identify the Fusarium species and their mycotoxin profiles associated to maize ear rot and kernel contamination in Iran, a wide sampling was carried out from field in ten major maize-producing provinces in Iran, during 2015 and 2016. From 182 samples of maize kernels, 551 strains were isolated and identified as belonging to Fusarium genus. Among the 234 representative strains identified at species level by translation elongation factor (EF-1α) sequences, the main Fusarium species were F. verticillioides and F. proliferatum, together representing 90% of the Iranian Fusarium population, and, to a lesser extent, F. incarnatum equiseti species complex (FIESC), F. thapsinum and F. redolens. Fumonisin (FBs) production by F. verticillioides and F. proliferatum representative strains was analysed, showing that all strains produced FB1. None of F. verticillioides strains produced FB2 nor FB3, while both FB2 and FB3 were produced only by F. proliferatum. Total mean of FBs production by F. verticillioides was higher than F. proliferatum. The occurrence of different Fusarium species on Iranian maize is reason of great concern because of the toxigenic risk associated to these species. Moreover, the diversity of the species identified increases the toxigenic risk associated to Fusarium contaminated maize kernels, because of the high possibility that a multi-toxin contamination can occur with harmful consequences on human and animal health.

scholarly journals Developments in Diagnosis of Visceral Leishmaniasis in the Elimination Era

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Om Prakash Singh ◽  
Shyam Sundar
Keyword(s):  
Visceral Leishmaniasis ◽  
Parasitic Infection ◽  
Clinical Manifestations ◽  
Cost Effective ◽  
Current Status ◽  
Effective Control ◽  
Diagnostic Tools ◽  
High Morbidity ◽  
Symptomatic Disease ◽  
Primary Health Care Centers

Visceral leishmaniasis (VL) is the most devastating parasitic infection worldwide causing high morbidity and mortality. Clinical presentation of VL ranges from asymptomatic or subclinical infection to severe and complicated symptomatic disease. A major challenge in the clinical management of VL is the weakness of health systems in disease endemic regions. People affected by VL mostly present to primary health care centers (PHCs), often late in their therapeutic itinerary. PHC physicians face a major challenge: they do not deal with a single disease issue but with patients presenting with complaints pointing to several diagnostic possibilities. Risk exists when some patients having less clinical manifestations are misdiagnosed. Therefore, field based accurate, sensitive, and cost effective rapid diagnostic tools that can detect disease in its mildest form are essential for effective control and reaching the goal of VL elimination. In this review, we discuss the current status and challenges of various diagnostic tools for the diagnosis of VL and assess their application in resource poor settings.

scholarly journals An Insight of Existing Research Methods towards Securing IoT Communication System

2020 ◽  
Vol 9 (4) ◽  
pp. 1362-1371
Keyword(s):  
Cost Effective ◽  
Research Direction ◽  
Security And Privacy ◽  
Current Status ◽  
Effective Control ◽  
Research Trend ◽  
Smart Device ◽  
Future Research ◽  
Individual Privacy ◽  
Iot Devices

IoT is an advanced analytics system that incorporates physical devices, intelligent technologies, and internet services to provide automation services and cost-effective productivity. The IoT oriented framework offers effective control, stabilized execution, and translucent services when it is applied to any enterprise. However, IoT devices are at risk of many security threats because they communicate and operate smart device services over wide distributed networks (i.e., insecure Internet channels). Over the past few years, it has been witnessed that several types of malicious activities have been tried to undermine the security and privacy of sensor networks as well as Internet-based applications. In order to protect IoT devices, many research works have been carried out to address potential security attack and to find an optimal way to overcome and limit the risk factors that affect security requirement and individual privacy. Therefore, this paper carries an assessment of the existing research works in order to understand better the root cause of the new possible threat and the challenges associated with IoT security. The paper also reflects the research trend to demonstrate the current status of security methods being developed till date. The prime motive of the present work is to reveal the current research gap and make an effective contribution to the future research direction.

scholarly journals Phosphate-Responsive Promoter of a Pichia pastoris Sodium Phosphate Symporter

2009 ◽  
Vol 75 (11) ◽  
pp. 3528-3534 ◽  
Author(s):  
Jungoh Ahn ◽  
Jiyeon Hong ◽  
Myongsoo Park ◽  
Hyeokweon Lee ◽  
Eungyo Lee ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Elongation Factor ◽  
Cost Effective ◽  
Specific Productivity ◽  
Translation Elongation Factor ◽  
Lipase Gene ◽  
Elongation Factor 1Α ◽  
Translation Elongation ◽  
Reading Frame ◽  
Translation Elongation Factor 1Α

ABSTRACT To develop a functional phosphate-regulated promoter in Pichia pastoris, a phosphate-responsive gene, PHO89, which encodes a putative sodium (Na+)-coupled phosphate symporter, was isolated. Sequencing analyses revealed a 1,731-bp open reading frame encoding a 576-amino-acid polypeptide with 12 putative transmembrane domains. The properties of the PHO89 promoter (PPHO89 ) were investigated using a bacterial lipase gene as a reporter in 5-liter jar fermentation experiments. PPHO89 was tightly regulated by phosphate and was highly activated when the cells were grown in a phosphate-limited external environment. Compared to translation elongation factor 1α and the glyceraldehyde-3-phosphate dehydrogenase promoter, PPHO89 exhibited strong transcriptional activity with higher specific productivity (amount of lipase produced/cell/h). Furthermore, a cost-effective and simple PPHO89 -based fermentation process was developed for industrial application. These results demonstrate the potential for efficient use of PPHO89 for controlled production of recombinant proteins in P. pastoris.

scholarly journals Diaporthe nebulae sp. nov. and First Report of D. cynaroidis, D. novem, and D. serafiniae on Grapevines in South Africa

Plant Disease ◽  
2019 ◽  
Vol 103 (5) ◽  
pp. 808-817 ◽  
Author(s):  
Palesa Lesuthu ◽  
Lizel Mostert ◽  
Christoffel F. J. Spies ◽  
Providence Moyo ◽  
Thierry Regnier ◽  
...  
Keyword(s):  
South Africa ◽  
Elongation Factor ◽  
Internal Transcribed Spacers ◽  
Current Status ◽  
Western Cape ◽  
Translation Elongation ◽  
5.8S Rrna ◽  
Elongation Factor 1 Alpha ◽  
Species Specific ◽  
First Time

Diaporthe species cause Phomopsis cane and leaf spot as well as Phomopsis dieback on grapevines. Symptoms of Phomopsis dieback have increasingly been observed over the past few years. In order to assess the current status of Diaporthe on grapevines in the Western Cape Province of South Africa, isolations were made from dormant grafted nursery vines, dormant rootstock canes, and dying or dead spurs of field vines. Cultures identified as Diaporthe based on morphological features were further identified to species level by sequencing the internal transcribed spacers (ITS) 1 and 2 and 5.8S rRNA and, for a representative subsample of isolates, the partial beta-tubulin (tub2) and translation elongation factor 1-alpha (EF1-α) genes. Phylogenetic analysis of the combined ITS, tub2, and EF1-α data revealed nine Diaporthe species associated with grapevines during this survey. One of these represents a new species, D. nebulae sp. nov., and three other species, namely D. novem, D. cynaroidis, and D. serafiniae, are reported on grapevines in South Africa for the first time. Species-specific primers were designed for PCR identification of D. ampelina, D. ambigua, and D. foeniculina. Pathogenicity studies conducted on detached grapevine shoots indicated D. ampelina, D. novem, and D. nebulae sp. nov. as the most virulent species.

Two-stage chemical-biological treatment at Thessaloniki greater area WWTP - experiences and potentials

1995 ◽  
Vol 32 (9-10) ◽  
pp. 75-84 ◽  
Author(s):  
A. D. Andreadakis ◽  
G. H. Kristensen ◽  
A. Papadopoulos ◽  
C. Oikonomopoulos
Keyword(s):  
Biological Treatment ◽  
Treatment Plant ◽  
Cost Effective ◽  
Primary Treatment ◽  
Effective Control ◽  
Two Stage ◽  
Effluent Quality ◽  
Filamentous Microorganisms ◽  
Sludge Settling ◽  
The City

The wastewater from the city of Thessaloniki is discharged without treatment to the nearby inner part of the Thessaloniki Gulf. The existing, since 1989, treatment plant offers only primary treatment and did not operate since the expected effluent quality is not suitable for safe disposal to the available recipients. Upgrading of the plant for advanced biological treatment, including seasonal nitrogen removal, is due from 1995. In the mean time, after minor modifications completed in February 1992, the existing plant was put into operation as a two-stage chemical-biological treatment plant for 40 000 m3 d−1, which corresponds to about 35% of the total sewage flow. The operational results obtained during the two years operation period are presented and evaluated. All sewage and sludge treatment units of the plant perform better than expected, with the exception of the poor sludge settling characteristics, due to severe and persistent bulking caused by excessive growth of filamentous microorganisms, particularly M. Parvicella. Effective control of the bulking problem could lead to more cost-effective operation and increased influent flows.

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