scholarly journals Large-Scale Screening of 239 Traditional Chinese Medicinal Plant Extracts for Their Antibacterial Activities against Multidrug-Resistant Staphylococcus aureus and Cytotoxic Activities

Pathogens ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 185 ◽  
Author(s):  
Gowoon Kim ◽  
Ren-You Gan ◽  
Dan Zhang ◽  
Arakkaveettil Kabeer Farha ◽  
Olivier Habimana ◽  
...  
Keyword(s):  
Large Scale ◽  
Salvia Miltiorrhiza ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Multidrug Resistant ◽  
Cytotoxic Activities ◽  
Phenolic Contents ◽  
Medicinal Plant Extracts ◽  
Low Cytotoxicity

Novel alternative antibacterial compounds have been persistently explored from plants as natural sources to overcome antibiotic resistance leading to serious foodborne bacterial illnesses. In this study, the ethanolic extracts from 239 traditional Chinese medicinal plants (TCMP)’ materials were screened to discover promising candidates that have strong antibacterial properties against multidrug-resistant Staphylococcus (S.) aureus and low cytotoxicity. The results revealed that 74 extracts exhibited good antibacterial activities (diameter of inhibition zone (DIZ) ≥ 15 mm). Furthermore, 18 extracts (DIZ ≥ 20 mm) were determined their minimum inhibitory concentrations (MIC) and minimum bactericide concentrations (MBC), ranging from 0.1 to 12.5 mg/mL and 0.78 to 25 mg/mL, respectively. In addition, most of the 18 extracts showed relatively low cytotoxicity (a median lethal concentration (LC50) >100 µg/mL). The 18 extracts were further determined to estimate possible correlation of their phenolic contents with antibacterial activity, and the results did not show any significant correlation. In conclusion, this study selected out some promising antibacterial TCMP extracts with low cytotoxicity, including Rhus chinensis Mill., Ilex rotunda Thunb., Leontice kiangnanensis P.L.Chiu, Oroxylum indicum Vent., Isatis tinctorial L., Terminalia chebula Retz., Acacia catechu (L.f.) Willd., Spatholobus suberectus Dunn, Rabdosia rubescens (Hemsl.) H.Hara, Salvia miltiorrhiza Bunge, Fraxinus fallax Lingelsh, Coptis chinensis Franch., Agrimonia Pilosa Ledeb., and Phellodendron chinense C.K.Schneid.

scholarly journals Synthesis, Structure Elucidation, Antibacterial Activities, and Synergistic Effects of Novel Juglone and Naphthazarin Derivatives Against Clinical Methicillin-Resistant Staphylococcus aureus Strains

2021 ◽  
Vol 9 ◽  
Author(s):  
Valentin Duvauchelle ◽  
Chaimae Majdi ◽  
David Bénimélis ◽  
Catherine Dunyach-Remy ◽  
Patrick Meffre ◽  
...  
Keyword(s):  
Synergistic Effects ◽  
Antibacterial Properties ◽  
Clinical Importance ◽  
Antibacterial Activities ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Global Public Health ◽  
Gram Positive ◽  
Drug Resistant Bacteria ◽  
Mrsa Strains

Infections caused by drug-resistant bacteria are a serious threat to human and global public health. Moreover, in recent years, very few antibiotics have been discovered and developed by pharmaceutical companies. Therefore, there is an urgent need to discover and develop new antibacterial agents to combat multidrug-resistant bacteria. In this study, two novel series of juglone/naphthazarin derivatives (43 compounds) were synthesized and evaluated for their antibacterial properties against various clinical and reference Gram-positive MSSA, clinical Gram-positive MRSA, and clinical and reference Gram-negative bacteria E. coli and P. aeruginosa. These strains are of clinical importance because they belong to ESKAPE pathogens. Compounds 3al, 5ag, and 3bg showed promising activity against clinical and reference MSSA (MIC: 1–8 µg/ml) and good efficacy against clinical MRSA (MIC: 2–8 µg/ml) strains. 5am and 3bm demonstrated better activity on both MSSA (MIC: 0.5 µg/ml) and MRSA (MIC: 2 µg/ml) strains. Their MICs were similar to those of cloxacillin against clinical MRSA strains. The synergistic effects of active compounds 3al, 5ag, 5am, 3bg, and 3bm were evaluated with reference antibiotics, and it was found that the antibiotic combination with 3bm efficiently enhanced the antimicrobial activity. Compound 3bm was found to restore the sensitivity of clinical MRSA to cloxacillin and enhanced the antibacterial activity of vancomycin when they were added together. In the presence of 3bm, the MIC values of vancomycin and cloxacillin were lowered up to 1/16th of the original MIC with an FIC index of 0.313. Moreover, compounds 3al, 5ag, 5am, 3bg, and 3bm did not present hemolytic activity on sheep red blood cells. In silico prediction of ADME profile parameter results for 3bm is promising and encouraging for further development.

scholarly journals Antibacterial properties of an Indian traditional medicinal plant, Rhynchosia scarabaeoides (L.) DC. (Fabaceae)

2011 ◽  
Vol 1 (1) ◽  
pp. 17 ◽  
Author(s):  
Surekha Challa ◽  
Kiran K. Rajam ◽  
Vishnu V. V. Satyanarayana Kasapu ◽  
Suresh Kumar Tanneeru ◽  
Venkata Siva Satyanarayana Kantamreddi
Keyword(s):  
Ethyl Acetate ◽  
Bacterial Infections ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Traditional Healers ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Plant Parts ◽  
Ethanol Extracts

<em>Rhynchosia scarabaeoides </em>(L.) DC plant parts are extensively used by traditional healers in India to treat a variety of bacterial diseases, such as dysentery, diarrohea and skin disorders. This article reports the antibacterial activities of n-hexane, ethyl acetate and ethanol extracts belonging to the leaf, stem and root parts of <em>R. scarabaeoides</em> against five bacterial strains, <em>Bacillus subtilis</em>, <em>Escherichia coli</em>, <em>Klebseilla pneumonia</em>, <em>Proteus vulgaris</em> and <em>Staphylococcus aureus</em>, using an agar gel diffusion method. The range of inhibition zone (IZ) was found to be 15-24 mm and the minimum inhibitory activity (MIC) was found to be 1 mL/well. The IZ was found to be higher in ethyl acetate extracts while this was moderate in ethanol extracts, and no activity was seen with n-hexane extracts or root extracts. The MIC value of leaf ethyl acetate extract was found to be 1 mg against bacterial strains <em>P. vulgaris</em> and <em>S. aureus</em>, whereas 2 mg was found against <em>B. subtilis</em>, <em>K. pneumoniae</em> and <em>E. coli</em>. These results support the traditional usage of R. scarabaeoides plant parts in the treatment of bacterial infections. Interestingly, this plant was screened for antibacterial activity for the first time and was found to be active. Detailed chemical investigations are, therefore, warranted.

scholarly journals Gram-scale synthesis of splat-shaped Ag–TiO2 nanocomposites for enhanced antimicrobial properties

2020 ◽  
Vol 11 ◽  
pp. 1119-1125
Author(s):  
Mohammad Jaber ◽  
Asim Mushtaq ◽  
Kebiao Zhang ◽  
Jindan Wu ◽  
Dandan Luo ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Multidrug Resistant ◽  
Bacterial Strains ◽  
Gram Negative ◽  
E Coli ◽  
Contagious Diseases ◽  
Good Biocompatibility

The control over contagious diseases caused by pathogenic organisms has become a serious health issue. The extensive usage of antibiotics has led to the development of multidrug-resistant bacterial strains. In this regard, metal-oxide-based antibacterial nanomaterials have received potential research interest due to the efficient prevention of microorganism growth. In this study, splat-shaped Ag–TiO2 nanocomposites (NCs) were synthesized on the gram scale and the enhanced antibacterial properties of TiO2 in the presence of silver were examined. The formation of Ag–TiO2 NCs was analyzed through various characterization techniques. The cell viability experimental results demonstrated that the Ag–TiO2 NCs have good biocompatibility. The antibacterial activity of the prepared Ag–TiO2 NCs was tested against the Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) bacterial strains. The Ag–TiO2 NCs exhibited promising and superior antibacterial properties compared to TiO2 nanospheres as confirmed by the bacterial growth and inhibition zone. The improvement in the antibacterial activity was attributed to the synergistic effect of the hybrid nature of TiO2 nanoparticles in the presence of Ag.

scholarly journals Antioxidant, Antibacterial, and Cytotoxic Activities of the EthanolicOriganum vulgareExtract and Its Major Constituents

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
John Coccimiglio ◽  
Misagh Alipour ◽  
Zi-Hua Jiang ◽  
Christine Gottardo ◽  
Zacharias Suntres
Keyword(s):  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
A549 Cells ◽  
Antibacterial Activities ◽  
Major Constituent ◽  
Cytotoxic Activities ◽  
Bacterial Strains ◽  
Spectroscopy Analysis ◽  
Perennial Shrub ◽  
Gas Chromatography Mass Spectroscopy

Oregano is a perennial shrub that grows in the mountains of the Mediterranean and Euro/Irano-Siberian regions. This study was conducted to identify the major constituents of the ethanolicOriganum vulgareextract and examine the cytotoxic, antioxidant, and antibacterial properties of the extract but more importantly the contribution of its specific major constituent(s) or their combination to the overall extract biological activity. Gas chromatography/mass spectroscopy analysis showed that the extract contained monoterpene hydrocarbons and phenolic compounds, the major ones being carvacrol and thymol and to a lesser extent p-cymene, 1-octacosanol, creosol, and phytol. A549 epithelial cells challenged with the extract showed a concentration-dependent increase in cytotoxicity. A combination of thymol and carvacrol at equimolar concentrations to those present in the extract was less cytotoxic. The A549 cells pretreated with nonlethal extract concentrations protected against hydrogen-peroxide-induced cytotoxicity, an antioxidant effect more effective than the combination of equimolar concentrations of thymol/carvacrol. Inclusion of p-cymene and/or 1-octacosanol did not alter the synergistic antioxidant effects of the carvacrol/thymol mixture. The extract also exhibited antimicrobial properties against Gram-positive and Gram-negative bacterial strains including clinical isolates. In conclusion, the oregano extract has cytotoxic, antioxidant, and antibacterial activities mostly attributed to carvacrol and thymol.

Characterization of Konjac Glucomannan Film Containing the Extracts of Atractylodes lancea and Saussurea lappa

2012 ◽  
Vol 506 ◽  
pp. 401-404
Author(s):  
T. Kaewsomboon ◽  
Komgrit Sawangkan ◽  
Chutimon Satirapipathkul
Keyword(s):  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Mean Value ◽  
Konjac Glucomannan ◽  
Saussurea Lappa ◽  
Atractylodes Lancea ◽  
The Mean

The antibacterial properties of konjac glucomannan (KGM) film containing two herb extracts (Atractylodes lancea and Saussurea lappa) at various concentrations was studied in this present work. This activity was tested against two pathogenic bacteria, Bacillus subtilis and Staphylococcus aureus. The results showed that the mean value of inhibition zone diameter of incorporated films with Saussurea lappa extracts were higher than those incorporating Atractylodes lancea extracts for all concentrations. The film incorporated with S. lappa extract had higher antibacterial activity against both bacteria than incorporated with A. lamcea. For all these levels, it can be seen that the KGM film had satisfactory physical properties and antibacterial activities.

scholarly journals Initial Evaluation of Antioxidant and Antibacterial Activities of Medicinal Plant Extracts in Vietnam

2021 ◽  
Vol 37 (3) ◽  
Author(s):  
Le Tuan Anh ◽  
Pham Thi Thanh Hang ◽  
Nguyen Thi Bich Loan ◽  
Le Quynh Mai
Keyword(s):  
Plant Extracts ◽  
Radical Scavenging ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Bacterial Strains ◽  
Medicinal Plant Extracts ◽  
Health Products ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay

The diversity of medicinal plants in Vietnam is a rich source to develop novel health products. This study evaluated the antioxidant and antimicrobial activities of ethanolic extracts of Pluchea indica, Drynaria fortunei, Stephania glabra, lemongrass and lime mixture, and ginger and kumquat mixture. The extract of ginger and kumquat mixture showed the highest percentage of free radical scavenging (85% at the concentration of 0.1 mg/ml), following by the extract of P. indica leaves and stem (60%). The plant extracts displayed antibacterial activity against Bacillus cereus, Staphylococcus aureus, and Vibrio parahaemolyticus at different levels in the agar well diffusion assay. The extract of S. glabra inhibited the growth of all bacterial strains. The mean of inhibition zone diameter, produced by S. glabra extract, was similar to or even significantly higher than that of control at particular concentrations.

scholarly journals Phenolic compound derived from microwave-assisted pyrolysis of coconut shell: Isolation and antibacterial activity testing

2020 ◽  
Vol 202 ◽  
pp. 10007
Author(s):  
Dwi Nuryana ◽  
Muhammad Fahrul Rahman Alim ◽  
Rendy Okta Loveyanto ◽  
Bayu Adi Wibowo ◽  
Raja Safazliana Binti Raja Sulong ◽  
...  
Keyword(s):  
Antibacterial Agent ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Coconut Shell ◽  
Total Phenolic ◽  
Effective Technique ◽  
E Coli ◽  
Phenolic Contents ◽  
Microwave Assisted ◽  
Phenolic Extract

Indonesia is rich in natural resources, coconut plantation being one of them. The coconut processing industry produces coconut shell (CS) waste. The most effective technique to increase the value of this waste is to convert CS biomass through pyrolysis process. This research focuses on intensification of conversion process of CS by Microwave-Assisted Pyrolysis (MAP) to obtain PA. PA contains phenolic compounds which have antibacterial properties so they can be formulated as an antibacterial agent. CS has moisture and ash content of 8.89% and 0.21%, respectively. PA was produced from the MAP of CS at 600W at 450℃ and 500oC for 10, 20, and 30 minutes. The PA was extracted using ethyl acetate to obtain phenolic contents. Optimum pyrolysis condition was found at 400oC for 30 minutes and yield of PA was determined at 32.20 g with total phenolic content (TPC) of 112.13 mg GAE/g. The inhibition zone of phenolic extract from coconut shell (PECS) using E. Coli was determined within 22-25 mm that quantitatively PECS can effectively kill bacteria. PECS by MAP and its aplication as an antibacterial agent has never been performed, so this work is an important contribution in the intensification of pyrolysis and in medical field.

Medicinal Plants: Antibacterial Effects and Chemical Composition of Essential Oil of Foeniculum vulgare

2017 ◽  
Vol 8 (01) ◽  
Author(s):  
Azadeh Foroughi ◽  
Pouya Pournaghi ◽  
Fariba Najafi ◽  
Akram Zangeneh ◽  
Mohammad Mahdi Zangeneh ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Medicinal Plants ◽  
Essential Oil ◽  
Antibacterial Properties ◽  
Screen Test ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Resistant Bacteria ◽  
Antibacterial Effects ◽  
Foeniculum Vulgare

Medicinal plants are considered modern resources for producing agents that could act as alternatives to antibiotics in demeanor of antibiotic-resistant bacteria. The aim of the study was to evaluate the chemical composition and antibacterial activities of essential oil of Foeniculum vulgare (FV) against Pseudomonas aeruginosa and Bacillus subtilis. Gas chromatography mass spectrometry was done to specify chemical composion. As a screen test to detect antibacterial properties of the essential oil, agar disk and agar well diffusion methods were employed. Macrobroth tube test was performed to determinate MIC. The results indicated that the most substance found in FV essential oil was Trans-anethole (47.41 %), also the essential oil of FV with 0.007 g/ml concentration has prevented P. aeruginosa and with 0.002 g/ml concentration has prevented B. subtilis from the growth. Thus, the research represents the antibacterial effects of the medical herb on test P. aeruginosa and B. subtilis. We believe that the article provide support to the antibacterial properties of the essential oil. The results indicate the fact that the essential oil from the plant can be useful as medicinal or preservatives composition.

scholarly journals Antibacterial Activity of Moroccan Zantaz Honey and the Influence of Its Physicochemical Parameters Using Chemometric Tools

2021 ◽  
Vol 11 (10) ◽  
pp. 4675
Author(s):  
Youssef Elamine ◽  
Hamada Imtara ◽  
Maria Graça Miguel ◽  
Ofélia Anjos ◽  
Letícia M. Estevinho ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Principal Component ◽  
Physicochemical Characteristics ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Coumaric Acid ◽  
Atlas Mountains ◽  
Methyl Syringate ◽  
Chemometric Tools

The emergence of multidrug-resistant bacteria has prompted the development of alternative therapies, including the use of natural products with antibacterial properties. The antibacterial properties of Zantaz honey produced in the Moroccan Atlas Mountains against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus was evaluated and analyzed using chemometric tools. Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) against S. aureus were the lowest (112.5 ± 54.5 mg/mL), revealing that this species was most sensitive to Zantaz honey. P. aeruginosa showed an intermediate sensitivity (MIC= 118.75 ± 51.9 mg/mL), while E. coli was the most resistant to treatment (MIC = 175 ± 61.2 mg/mL). Content of monosaccharides, certain minerals, and phenolic compounds correlated with antibacterial activity (p < 0.05). Principal component analysis of physicochemical characteristics and antibacterial activity indicated that the parameters most associated with antibacterial activity were color, acidity, and content of melanoidins, fructose, epicatechin, methyl syringate, 4-coumaric acid, and 3-coumaric acid.

Antibacterial properties of phenothiazine derivatives against multidrug‐resistant Acinetobacter baumannii strains

2021 ◽  
Author(s):  
Laura Aguilar‐Vega ◽  
Luis Esaú López‐Jácome ◽  
Bernardo Franco ◽  
Sergio Muñoz‐Carranza ◽  
Naurú Vargas‐Maya ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Antibacterial Properties ◽  
Multidrug Resistant ◽  
Phenothiazine Derivatives
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