scholarly journals Non-Specific Lipid Transfer Proteins in Triticum kiharae Dorof. et Migush.: Identification, Characterization and Expression Profiling in Response to Pathogens and Resistance Inducers

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 221 ◽  
Author(s):  
Odintsova ◽  
Slezina ◽  
Istomina ◽  
Korostyleva ◽  
Kovtun ◽  
...  
Keyword(s):  
Fusarium Sambucinum ◽  
Lipid Transfer ◽  
Rna Seq ◽  
Lipid Transfer Proteins ◽  
Specific Expression ◽  
Resistance Development ◽  
Physiological Processes ◽  
Resistance Inducers ◽  
Specific Lipid

Non-specific lipid-transfer proteins (nsLTPs) represent a family of plant antimicrobial peptides (AMPs) implicated in diverse physiological processes. However, their role in induced resistance (IR) triggered by non-pathogenic fungal strains and their metabolites is poorly understood. In this work, using RNA-seq data and our AMP search pipeline, we analyzed the repertoire of nsLTP genes in the wheat Triticum kiharae and studied their expression in response to Fusarium oxysporum infection and treatment with the intracellular metabolites of Fusarium sambucinum FS-94. A total of 243 putative nsLTPs were identified, which were classified into five structural types and characterized. Expression analysis showed that 121 TkLTPs including sets of paralogs with identical mature peptides displayed specific expression patters in response to different treatments pointing to their diverse roles in resistance development. We speculate that upregulated nsLTP genes are involved in protection due to their antimicrobial activity or signaling functions. Furthermore, we discovered that in IR-displaying plants, a vast majority of nsLTP genes were downregulated, suggesting their role as negative regulators of immune mechanisms activated by the FS-94 elicitors. The results obtained add to our knowledge of the role of nsLTPs in IR and provide candidate molecules for genetic engineering of crops to enhance disease resistance.

scholarly journals The Role of Lipid Transfer Proteins as Food and Pollen Allergens Outside the Mediterranean Area

2021 ◽  
Vol 21 (2) ◽  
Author(s):  
Stephan Scheurer ◽  
Ronald van Ree ◽  
Stefan Vieths
Keyword(s):  
Mediterranean Area ◽  
Food Allergies ◽  
Lipid Transfer ◽  
Pollen Allergens ◽  
Lipid Transfer Proteins ◽  
High Exposure ◽  
The Mediterranean ◽  
Specific Lipid ◽  
Pru P 3

Abstract Purpose of Review To provide an overview of the prevalence and clinical manifestation of non-specific lipid transfer proteins (LTP)-mediated allergies outside the Mediterranean area and to address potential reasons for the different geographical significance of LTP-driven allergies. Recent Findings LTPs are major allergens in the Mediterranean area, which frequently can elicit severe reactions. Pru p 3 the LTP from peach is reported as genuine allergen and is considered a prototypic marker for LTP-mediated allergies. However, both food and pollen LTP allergies exist outside the Mediterranean area, but with lower clinical significance, different immunogenicity, and less clarified role. Summary Evidence has been reported that in areas with high exposure to pollen, in particular to mugwort, pollen-derived LTPs can act as a primary sensitizer to trigger secondary food allergies. Co-sensitization to unrelated allergens might be causative for less severe reactions in response to LTPs. However, the reason for the geographical different sensitization patterns to LTPs remains unclear.

scholarly journals Genome-wide identification and characterization of non-specific lipid transfer proteins in cabbage

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5379 ◽  
Author(s):  
Jialei Ji ◽  
Honghao Lv ◽  
Limei Yang ◽  
Zhiyuan Fang ◽  
Mu Zhuang ◽  
...  
Keyword(s):  
Tertiary Structure ◽  
Expression Patterns ◽  
Lipid Transfer ◽  
Lipid Transfer Proteins ◽  
Specific Expression ◽  
Functional Studies ◽  
Genome Wide ◽  
Resistance To Stress ◽  
Physical And Chemical ◽  
Specific Lipid

Plant non-specific lipid transfer proteins (nsLTPs) are a group of small, secreted proteins that can reversibly bind and transport hydrophobic molecules. NsLTPs play an important role in plant development and resistance to stress. To date, little is known about the nsLTP family in cabbage. In this study, a total of 89 nsLTP genes were identified via comprehensive research on the cabbage genome. These cabbage nsLTPs were classified into six types (1, 2, C, D, E and G). The gene structure, physical and chemical characteristics, homology, conserved motifs, subcellular localization, tertiary structure and phylogeny of the cabbage nsLTPs were comprehensively investigated. Spatial expression analysis revealed that most of the identified nsLTP genes were positively expressed in cabbage, and many of them exhibited patterns of differential and tissue-specific expression. The expression patterns of the nsLTP genes in response to biotic and abiotic stresses were also investigated. Numerous nsLTP genes in cabbage were found to be related to the resistance to stress. Moreover, the expression patterns of some nsLTP paralogs in cabbage showed evident divergence. This study promotes the understanding of nsLTPs characteristics in cabbage and lays the foundation for further functional studies investigating cabbage nsLTPs.

scholarly journals Non‐specific lipid‐transfer proteins: Allergen structure and function, cross‐reactivity, sensitization, and epidemiology

2021 ◽  
Vol 11 (3) ◽  
Author(s):  
Isabel J. Skypala ◽  
Ricardo Asero ◽  
Domingo Barber ◽  
Lorenzo Cecchi ◽  
Arazeli Diaz Perales ◽  
...  
Keyword(s):  
Cross Reactivity ◽  
Structure And Function ◽  
Lipid Transfer ◽  
Lipid Transfer Proteins ◽  
Allergen Structure ◽  
And Function ◽  
Specific Lipid

scholarly journals Structural Characterization of Act c 10.0101 and Pun g 1.0101—Allergens from the Non-Specific Lipid Transfer Protein Family

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 256
Author(s):  
Andrea O’Malley ◽  
Swanandi Pote ◽  
Ivana Giangrieco ◽  
Lisa Tuppo ◽  
Anna Gawlicka-Chruszcz ◽  
...  
Keyword(s):  
Immunoglobulin E ◽  
Lipid Transfer Protein ◽  
Mass Spectrometry Analysis ◽  
Lipid Transfer ◽  
Helical Structures ◽  
Spectrometry Analysis ◽  
X Ray Crystallography ◽  
Transfer Protein ◽  
Specific Lipid

(1) Background: Non-specific lipid transfer proteins (nsLTPs), which belong to the prolamin superfamily, are potent allergens. While the biological role of LTPs is still not well understood, it is known that these proteins bind lipids. Allergen nsLTPs are characterized by significant stability and resistance to digestion. (2) Methods: nsLTPs from gold kiwifruit (Act c 10.0101) and pomegranate (Pun g 1.0101) were isolated from their natural sources and structurally characterized using X-ray crystallography (3) Results: Both proteins crystallized and their crystal structures were determined. The proteins have a very similar overall fold with characteristic compact, mainly α-helical structures. The C-terminal sequence of Act c 10.0101 was updated based on our structural and mass spectrometry analysis. Information on proteins’ sequences and structures was used to estimate the risk of cross-reactive reactions between Act c 10.0101 or Pun g 1.0101 and other allergens from this family of proteins. (4) Conclusions: Structural studies indicate a conformational flexibility of allergens from the nsLTP family and suggest that immunoglobulin E binding to some surface regions of these allergens may depend on ligand binding. Both Act c 10.0101 and Pun g 1.0101 are likely to be involved in cross-reactive reactions involving other proteins from the nsLTP family.

Role of plant lipid transfer proteins in plant cell physiology—A concise review

Peptides ◽  
2007 ◽  
Vol 28 (5) ◽  
pp. 1144-1153 ◽  
Author(s):  
André de Oliveira Carvalho ◽  
Valdirene Moreira Gomes
Keyword(s):  
Plant Cell ◽  
Cell Physiology ◽  
Lipid Transfer ◽  
Lipid Transfer Proteins ◽  
Plant Lipid ◽  
Concise Review

scholarly journals Epitope Mapping for the Non-Specific Lipid Transfer Proteins (nsLTP) Among Peanut Allergic Patients

2018 ◽  
Vol 141 (2) ◽  
pp. AB239
Author(s):  
Christina M. Kronfel ◽  
Hsiaopo Cheng ◽  
Barry K. Hurlburt ◽  
Reyna J. Simon ◽  
Soheila J. Maleki
Keyword(s):  
Epitope Mapping ◽  
Lipid Transfer ◽  
Lipid Transfer Proteins ◽  
Specific Lipid

scholarly journals Genome-wide identification and analysis of non-specific Lipid Transfer Proteins in hexaploid wheat

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Allan Kouidri ◽  
Ryan Whitford ◽  
Radoslaw Suchecki ◽  
Elena Kalashyan ◽  
Ute Baumann
Keyword(s):  
Hexaploid Wheat ◽  
Lipid Transfer ◽  
Lipid Transfer Proteins ◽  
Genome Wide ◽  
Specific Lipid
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