scholarly journals Genetic Analysis of Avian Gyrovirus 2 Variant-Related Gyrovirus Detected in Farmed King Ratsnake (Elaphe carinata): The First Report from China

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 185 ◽  
Author(s):  
Qianqian Wu ◽  
Xin Xu ◽  
Qinxi Chen ◽  
Jun Ji ◽  
Yunchao Kan ◽  
...  
Keyword(s):  
Open Reading Frames ◽  
Health Concern ◽  
First Report ◽  
Chicken Infectious Anemia Virus ◽  
Positive Rate ◽  
Chicken Infectious Anemia ◽  
Elaphe Carinata ◽  
Anemia Virus ◽  
Overlapping Open Reading Frames ◽  
Reference Strains

Avian gyrovirus 2 (AGV2), which is similar to chicken infectious anemia virus, is a new member of the genus Gyrovirus. AGV2 has been detected not only in chicken but also in human tissues and feces. This study analyzed 91 samples (8 from liver tissue and 83 from fecal samples) collected from king ratsnakes (Elaphe carinata) from 7 separate farms in Hubei and Henan, China, for AGV2 DNA using PCR. The results demonstrated a low positive rate of AGV2 (6.59%, 6/91) in the snakes, and all the positive samples were collected from the same farm. The AGV2 strain HB2018S1 was sequenced, and its 2376 nt genome comprised three partially overlapping open reading frames: VP1, VP2, and VP3. Phylogenetic analysis revealed that the HB2018S1 and NX1506-1 strains from chickens in China belong to the same clade and that they have a nucleotide identity as high as 99.5%. Additionally, recombination analysis showed that HB2018S1 might originate from the recombination of viruses similar to those detected in chickens and a ferret. A total of 10 amino acid mutation sites (44(R/K), 74(T/A), 256 (C/R), 279(L/Q), and 373(V/A) in AGV2 VP1; 60(I/T), 125(T/I), 213(D/N), and 215(L/S) in AGV2 VP2; and 83(H/Y) in AGV2 VP3) different from those observed in most reference strains were found in the genome of HB2018S1, indicating that the differences may be related to a transboundary movement among hosts, which needs further elucidation. To the best of our knowledge, this study is the first report on an AGV2-infected poikilotherm, suggesting that cross-host transmission of viruses with circular single-stranded DNA genomes would be a public health concern.

scholarly journals Emergence and Genetic Analysis of Avian Gyrovirus 2 Variants-RelatedGyrovirusin Farmed King-ratsnakes (Elaphe carinata): the First Report in China

2019 ◽  
Author(s):  
Qianqian Wu ◽  
Qinxi Chen ◽  
Wen Hu ◽  
Xueyu Wang ◽  
Jun Ji
Keyword(s):  
Mainland China ◽  
Open Reading Frames ◽  
Health Concern ◽  
Phylogenetic Tree Analysis ◽  
First Report ◽  
Specific Pcr ◽  
Positive Rate ◽  
Live Poultry ◽  
Elaphe Carinata ◽  
Anemia Virus

ABSTRACTAvian gyrovirus 2 (AGV2), which is similar to chicken infectious anemia virus, is a new member of theCircovirusgenus. AGV2 has been detected not only in chicken but also in human tissues and feces. In this study, a total of 91 samples (8 liver tissues and 83 faecal samples) collected from king-ratsnakes (Elaphe carinata) at 7 separate farms in Hubei and Henan, China, were analyzed to detect AGV2 DNA via specific PCR. The results indicated a low positive rate of AGV2 (6.59%, 6/91) in the studied animals, and all of the positive samples were from the same farm. The AGV2 strain HB2018S1 was sequenced, and the genome with a total length of 2376 nt contained three partially overlapping open reading frames: VP1, VP2, and VP3. Phylogenetic tree analysis revealed that the HB2018S1, NX1506-1 strain from chickens in China belong to the same clade, with nucleotide homology as high as 99.5%. In total, 10 amino acid mutation sites, including 44R/K, 74T/A, 256 C/R, 279L/Q, and 373V/A in AGV2 VP1; 60I/T, 125T/I, 213D/N, and 215L/S in AGV2 VP2; and 83H/Y in AGV2 VP3, were found in the genome of HB2018S1 that were different from those observed in most reference strains, suggesting that the differences are related to an transboundary movement among hosts which needs to be further elucidated.IMPORTANCERecently, AGV2 has been detected in live poultry markets and human blood in mainland China. Previous findings indicated future studies should investigate the large geographic distribution of AGV2 and monitor the variants, the host range, and the associated diseases. To the best of our knowledge, this study is the first report on AGV2 infected poikilotherm, suggested that cross-host transmission of viruses with circular single-stranded DNA genomes would be a public health concern.

scholarly journals Genetic Analysis of Two Chicken Infectious Anemia Virus Variants-RelatedGyrovirusin Stray Mice and Dogs: The First Report in China, 2015

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Lichun Fang ◽  
Yang Li ◽  
Yixin Wang ◽  
Jiayuan Fu ◽  
Shuai Cui ◽  
...  
Keyword(s):  
Fecal Samples ◽  
First Report ◽  
Stray Cats ◽  
Detection Program ◽  
Significant Difference ◽  
Chicken Infectious Anemia Virus ◽  
Chicken Infectious Anemia ◽  
Blood Specimens ◽  
Anemia Virus ◽  
Relationship Of

Chicken infectious anemia virus (CIAV) causes acute viral infection in chickens worldwide. It can infect chickens of all ages, but the disease is seen only in young chickens and is characterized by hemorrhagic lesions in the muscles, atrophic changes in the lymphoid organs, aplastic bone marrow, and immunosuppression causing increased mortality. Previous studies have demonstrated that CIAV can be isolated from blood specimens of humans and fecal samples of stray cats. In the present study, two variants of CIAV were isolated from fecal samples of mice (CIAV-Mouse) and stray dogs (CIAV-Dog), respectively. The genome of the two CIAV variants was sequenced and the results of the recombination detection program suggested that the CIAV-Dog strain could be a recombinant viral strain generated from parental CIAV strains, AB119448 and GD-1-12, with high confidence. Particularly, these findings were obtained from the comparison of genetic diversity and the relationship of CIAV between different hosts. This is the first report indicating that there is a significant difference in the number of transcription factor binding sites in CIAV noncoding regions from different hosts. Further studies are required to investigate the large geographic distribution of CIAV and monitor the variants, host range, and associated diseases.

SEROLOGICAL STUDIES ON CHICKEN INFECTIOUS ANEMIA VIRUS IN GHARBEIA PROVINCE

2008 ◽  
Vol 6 (2) ◽  
pp. 196-208
Author(s):  
Moshira, A. El-abasy ◽  
Gamal, R. Hassab El-nabby ◽  
Ahmed, S. Hegasy ◽  
Salwa, M. Helmy
Keyword(s):  
Chicken Infectious Anemia Virus ◽  
Serological Studies ◽  
Chicken Infectious Anemia ◽  
Anemia Virus

scholarly journals Genetic Heterogeneity among Chicken Infectious Anemia Viruses Detected in Italian Fowl

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 944
Author(s):  
Giulia Quaglia ◽  
Giulia Mescolini ◽  
Elena Catelli ◽  
Giacomo Berto ◽  
Filippo Muccioli ◽  
...  
Keyword(s):  
Dna Amplification ◽  
Molecular Characteristics ◽  
Distribution Map ◽  
Present Survey ◽  
Broiler Breeder ◽  
Non Invasive ◽  
Chicken Infectious Anemia Virus ◽  
Chicken Infectious Anemia ◽  
Anemia Virus ◽  
Backyard Chicken

Chicken infectious anemia virus (CIAV) is a pathogen of chickens associated with immunosuppression and with a disease named chicken infectious anemia. The present survey reports an epidemiological study on CIAV distribution in Italian broiler, broiler breeder and backyard chicken flocks. Twenty-five strains were detected by a specifically developed nested PCR protocol, and molecularly characterized by partial VP1 gene or complete genome sequencing. Viral DNA amplification was successfully obtained from non-invasive samples such as feathers and environmental dust. Sequence and phylogenetic analysis showed the circulation of field or potentially vaccine-derived strains with heterogeneous sequences clustered into genogroups II, IIIa, and IIIb. Marker genome positions, reported to be correlated with CIAV virulence, were evaluated in field strains. In conclusion, this is the first survey focused on the molecular characteristics of Italian CIAVs, which have proved to be highly heterogeneous, implementing at the same time a distribution map of field viruses worldwide.

scholarly journals Negative modulation of the chicken infectious anemia virus promoter by COUP-TF1 and an E box-like element at the transcription start site binding δEF1

2008 ◽  
Vol 89 (12) ◽  
pp. 2998-3003 ◽  
Author(s):  
Myrna M. Miller ◽  
Keith W. Jarosinski ◽  
Karel A. Schat
Keyword(s):  
Fluorescent Protein ◽  
Transcription Start ◽  
Upstream Promoter ◽  
Chicken Infectious Anemia Virus ◽  
E Box ◽  
Promoter Construct ◽  
Chicken Infectious Anemia ◽  
Lmh Cells ◽  
Green Fluorescent ◽  
Anemia Virus

Expression of enhanced green fluorescent protein (EGFP) under control of the promoter-enhancer of chicken infectious anemia virus (CAV) is increased in an oestrogen receptor-enhanced cell line when treated with oestrogen and the promoter-enhancer binds unidentified proteins that recognize a consensus oestrogen response element (ERE). Co-transfection assays with the CAV promoter and the nuclear receptor chicken ovalbumin upstream promoter transcription factor 1 (COUP-TF1) showed that expression of EGFP was decreased by 50 to 60 % in DF-1 and LMH cells. The CAV promoter that included sequences at and downstream of the transcription start point had less expression than a short promoter construct. Mutation of a putative E box at this site restored expression levels. Electromobility shift assays showed that the transcription regulator delta-EF1 (δEF1) binds to this E box region. These findings indicate that the CAV promoter activity can be affected directly or indirectly by COUP-TF1 and δEF1.

Chicken Infectious Anemia Virus Infection in Israeli Commercial Flocks: Virus Amplification, Clinical Signs, Performance, and Antibody Status

10.1637/7072 ◽  
2004 ◽  
Vol 48 (1) ◽  
pp. 108-118 ◽  
Author(s):  
I. Davidson ◽  
M. Kedem ◽  
H. Borochovitz ◽  
N. Kass ◽  
G. Ayali ◽  
...  
Keyword(s):  
Virus Infection ◽  
Clinical Signs ◽  
Chicken Infectious Anemia Virus ◽  
Chicken Infectious Anemia ◽  
Anemia Virus

Immune Complex Vaccines for Chicken Infectious Anemia Virus

2011 ◽  
Vol 6 (1) ◽  
pp. e21-e22
Author(s):  
Karel A Schat ◽  
Nelson Rodrigo da Silva Martins ◽  
Priscilla H O'Connell ◽  
Michael S Piepenbrink
Keyword(s):  
Immune Complex ◽  
Chicken Infectious Anemia Virus ◽  
Chicken Infectious Anemia ◽  
Anemia Virus

scholarly journals Synergetic pathogenicity of Newcastle disease vaccines LaSota strain and contaminated chicken infectious anemia virus

2019 ◽  
Vol 98 (5) ◽  
pp. 1985-1992 ◽  
Author(s):  
Qi Su ◽  
Tuanjie Wang ◽  
Fanfeng Meng ◽  
Zhizhong Cui ◽  
Shuang Chang ◽  
...  
Keyword(s):  
Newcastle Disease ◽  
Chicken Infectious Anemia Virus ◽  
Chicken Infectious Anemia ◽  
Anemia Virus
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