scholarly journals Importance of B Lymphocytes and the IgG-Binding Protein Sbi in Staphylococcus aureus Skin Infection

Pathogens ◽  
2016 ◽  
Vol 5 (1) ◽  
pp. 12 ◽  
Author(s):  
Fan Zhao ◽  
Anita Chong ◽  
Christopher Montgomery
Keyword(s):  
Staphylococcus Aureus ◽  
B Lymphocytes ◽  
Skin Infection ◽  
Binding Protein ◽  
Igg Binding

Synthesis and mutagenesis of an IgG-binding protein based upon protein A of Staphylococcus aureus

1991 ◽  
Vol 4 (8) ◽  
pp. 963-970 ◽  
Author(s):  
Andrew G. Popplewell ◽  
Micheal G. Gore ◽  
Michael Scawen ◽  
Tony Atkinson
Keyword(s):  
Staphylococcus Aureus ◽  
Binding Protein ◽  
Protein A ◽  
Igg Binding

pH-sensitive interactions between IgG and a mutated IgG-binding protein based upon two B domains of Protein A from Staphylococcus aureus

1992 ◽  
Vol 5 (6) ◽  
pp. 577-582 ◽  
Author(s):  
Michael G. Gore ◽  
William F. Ferris ◽  
Andrew G. Popplewell ◽  
Michael Scawen ◽  
Tony Atkinson
Keyword(s):  
Staphylococcus Aureus ◽  
Binding Protein ◽  
Protein A ◽  
Ph Sensitive ◽  
Igg Binding

scholarly journals A second IgG-binding protein in Staphylococcus aureus

Microbiology ◽  
1998 ◽  
Vol 144 (4) ◽  
pp. 985-991 ◽  
Author(s):  
L. Zhang ◽  
K. Jacobsson ◽  
J. Vasi ◽  
M. Lindberg ◽  
L. Frykberg
Keyword(s):  
Staphylococcus Aureus ◽  
Binding Protein ◽  
Igg Binding

scholarly journals Relative Quantitative Comparisons of the Extracellular Protein Profiles of Staphylococcus aureus UAMS-1 and Its sarA, agr, and sarA agr Regulatory Mutants Using One-Dimensional Polyacrylamide Gel Electrophoresis and Nanocapillary Liquid Chromatography Coupled with Tandem Mass Spectrometry

2008 ◽  
Vol 190 (15) ◽  
pp. 5265-5278 ◽  
Author(s):  
Richard C. Jones ◽  
Joanna Deck ◽  
Ricky D. Edmondson ◽  
Mark E. Hart
Keyword(s):  
Mass Spectrometry ◽  
Staphylococcus Aureus ◽  
Liquid Chromatography ◽  
Gel Electrophoresis ◽  
Binding Protein ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
One Dimensional ◽  
Igg Binding ◽  
Relative Abundances

ABSTRACT One-dimensional polyacrylamide gel electrophoresis followed by nanocapillary liquid chromatography coupled with mass spectrometry was used to analyze proteins isolated from Staphylococcus aureus UAMS-1 after 3, 6, 12, and 24 h of in vitro growth. Protein abundance was determined using a quantitative value termed normalized peptide number, and overall, proteins known to be associated with the cell wall were more abundant early on in growth, while proteins known to be secreted into the surrounding milieu were more abundant late in growth. In addition, proteins from spent media and cell lysates of strain UAMS-1 and its isogenic sarA, agr, and sarA agr regulatory mutant strains during exponential growth were identified, and their relative abundances were compared. Extracellular proteins known to be regulated by the global regulators sarA and agr displayed protein levels in accordance with what is known regarding the effects of these regulators. For example, cysteine protease (SspB), endopeptidase (SspA), staphopain (ScpA), and aureolysin (Aur) were higher in abundance in the sarA and sarA agr mutants than in strain UAMS-1. The immunoglobulin G (IgG)-binding protein (Sbi), immunodominant staphylococcal antigen A (IsaA), IgG-binding protein A (Spa), and the heme-iron-binding protein (IsdA) were most abundant in the agr mutant background. Proteins whose abundance was decreased in the sarA mutant included fibrinogen-binding protein (Fib [Efb]), IsaA, lipase 1 and 2, and two proteins identified as putative leukocidin F and S subunits of the two-component leukotoxin family. Collectively, this approach identified 1,263 proteins (matches of two peptides or more) and provided a convenient and reliable way of identifying proteins and comparing their relative abundances.

scholarly journals GSDMD contributes to host defence against Staphylococcus aureus skin infection by suppressing the Cxcl1–Cxcr2 axis

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Zhen-Zhen Liu ◽  
Yong-Jun Yang ◽  
Feng-Hua Zhou ◽  
Ke Ma ◽  
Xiao-Qi Lin ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Skin Infection ◽  
Bacterial Colonization ◽  
Critical Role ◽  
Host Defence ◽  
Skin Damage ◽  
Microbial Infection ◽  
Caspase 1 ◽  
Deficient Mice

AbstractGasdermin D (GSDMD), a member of the gasdermin protein family, is a caspase substrate, and its cleavage is required for pyroptosis and IL-1β secretion. To date, the role and regulatory mechanism of GSDMD during cutaneous microbial infection remain unclear. Here, we showed that GSDMD protected against Staphylococcus aureus skin infection by suppressing Cxcl1–Cxcr2 signalling. GSDMD deficiency resulted in larger abscesses, more bacterial colonization, exacerbated skin damage, and increased inflammatory cell infiltration. Although GSDMD deficiency resulted in defective IL-1β production, the critical role of IL-1β was counteracted by the fact that Caspase-1/11 deficiency also resulted in less IL-1β production but did not aggravate disease severity during S. aureus skin infection. Interestingly, GSDMD-deficient mice had increased Cxcl1 secretion accompanied by increased recruitment of neutrophils, whereas Caspase-1/11-deficient mice presented similar levels of Cxcl1 and neutrophils as wild-type mice. Moreover, the absence of GSDMD promoted Cxcl1 secretion in bone marrow-derived macrophages induced by live, dead, or different strains of S. aureus. Corresponding to higher transcription and secretion of Cxcl1, enhanced NF-κB activation was shown in vitro and in vivo in the absence of GSDMD. Importantly, inhibiting the Cxcl1–Cxcr2 axis with a Cxcr2 inhibitor or anti-Cxcl1 blocking antibody rescued host defence defects in the GSDMD-deficient mice. Hence, these results revealed an important role of GSDMD in suppressing the Cxcl1–Cxcr2 axis to facilitate pathogen control and prevent tissue damage during cutaneous S. aureus infection.

scholarly journals Staphylococcus aureus uses the ArlRS and MgrA cascade to regulate immune evasion during skin infection

Cell Reports ◽  
2021 ◽  
Vol 36 (4) ◽  
pp. 109462
Author(s):  
Jakub M. Kwiecinski ◽  
Rachel M. Kratofil ◽  
Corey P. Parlet ◽  
Bas G.J. Surewaard ◽  
Paul Kubes ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Immune Evasion ◽  
Skin Infection
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