scholarly journals Effect of Processing Intensity on Immunologically Active Bovine Milk Serum Proteins

Nutrients ◽  
2017 ◽  
Vol 9 (9) ◽  
pp. 963 ◽  
Author(s):  
Tabea Brick ◽  
Markus Ege ◽  
Sjef Boeren ◽  
Andreas Böck ◽  
Erika von Mutius ◽  
...  
Keyword(s):  
Serum Proteins ◽  
Bovine Milk ◽  
Milk Serum

Characterizing the changes of bovine milk serum proteins after simulated industrial processing

LWT ◽  
2020 ◽  
Vol 133 ◽  
pp. 110101
Author(s):  
Yaowei Liu ◽  
Wenjin Zhang ◽  
Lina Zhang ◽  
Kasper Hettinga ◽  
Peng Zhou
Keyword(s):  
Serum Proteins ◽  
Bovine Milk ◽  
Industrial Processing ◽  
Milk Serum

scholarly journals Changes over lactation in breast milk serum proteins involved in the maturation of immune and digestive system of the infant

Data in Brief ◽  
2016 ◽  
Vol 7 ◽  
pp. 362-365 ◽  
Author(s):  
Lina Zhang ◽  
Marita de Waard ◽  
Hester Verheijen ◽  
Sjef Boeren ◽  
Jos A. Hageman ◽  
...  
Keyword(s):  
Breast Milk ◽  
Digestive System ◽  
Serum Proteins ◽  
Milk Serum

Observations on the influence of high cell count on lipolysis in bovine milk

1979 ◽  
Vol 46 (3) ◽  
pp. 453-462 ◽  
Author(s):  
Amer M. A. Salih ◽  
Malcolm Anderson
Keyword(s):  
Cell Count ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Bovine Milk ◽  
Skim Milk ◽  
High Cell ◽  
Lipoprotein Lipase Activity ◽  
Milk Serum ◽  
Cell Counts ◽  
And Control

SummaryThe effect that changes in composition which occur in milks possessing high cell counts have on milk lipolysis has been investigated. High cell counts were produced either by intramammary infusion of Escherichia coli endotoxin or Streptococcus agalactiae or by addition of washed cells which were isolated from milk obtained from quarters infused with endotoxin. Free fatty acid levels in milk were measured in terms of acid degree value (ADV) either as initial ADV measured immediately after milking or ADV developed after a prescribed incubation period.There was an increase in initial ADV after the infusion either of endotoxin or of Str. agalactiae relative to a control quarter. This increase appeared to be associated with changes in cell count, but in absolute terms the influence of cells on ADV became less as cell count increased. Neither type of infusion had any effect on lipoprotein lipase activity. The addition of washed cells to normal milk resulted in an increase in developed ADV, but the increment was not as large as that produced by the addition of 1% blood serum. When cream and skim-milk from endotoxin-treated quarters and control quarters were mixed in different combinations with and without additional cells, developed ADV was higher in those samples containing endotoxin cream and those with added cells. Milk from a quarter treated with endotoxin was diluted with its own skim-milk to produce different cell counts and ADV was determined after various time intervals at 4 and 37 °C. Lipolysis increased with increasing cell count, but a depression in lipolytic rate was noted after incubation for 6 h at 4 °C and 20 min at 37 °C.The proportion of skim-milk lipoprotein lipase activity in milk serum was larger both in milks possessing high cell counts and in normal milk adjusted to between 5 and 20 mM-NaCl by addition of solid NaCl. These levels of NaCl inhibited lipolysis.The possible direct and indirect effects of high cell count on milk lipolysis are discussed.

Factors affecting the distribution of lipoprotein lipase activity between serum and casein micelles in bovine milk

1982 ◽  
Vol 49 (1) ◽  
pp. 51-59 ◽  
Author(s):  
Malcolm Anderson
Keyword(s):  
Blood Serum ◽  
Lipoprotein Lipase ◽  
Post Partum ◽  
Bovine Milk ◽  
Casein Micelles ◽  
Factors Affecting ◽  
Milk Serum ◽  
Heparin Treatment ◽  
Micellar Casein ◽  
Consistent Increase

SUMMARYThe influence of mastitis and early lactation, and the effect of treating milk with heparin, blood serum and trypsin, on the proportion of lipoprotein lipase (LPL) activity in milk serum was investigated. The relative importance of milk serum LPL and LPL bound to micellar casein in promoting lipolysis was also examined. Colostrum contained LPL activity, 45% of which was found in the serum phase in samples obtained from the first milking post partum, but this value fell to 34% in samples taken 24 h later. The proportion of serum LPL was also increased in milks from quarters infected with Staphylococcus aureus, but not after overnight treatment of normal milk at 4 °C with 5% (w/v) blood serum or 2 µg/ml trypsin. The addition of 5 µg/ml heparin resulted in a consistent increase in serum LPL which varied between 14 and 50% of total milk LPL. Heparin did not release all the enzyme bound to casein micelles even after a second heparin treatment of resuspended micelles. Serum LPL was more effective in promoting lipolysis and was more responsive to blood serum activation than LPL bound to casein micelles. Lipolysis increased after heparin treatment but the increase was not related to serum LPL activity.

Changes over lactation in breast milk serum proteins involved in the maturation of immune and digestive system of the infant

2016 ◽  
Vol 147 ◽  
pp. 40-47 ◽  
Author(s):  
Lina Zhang ◽  
Marita de Waard ◽  
Hester Verheijen ◽  
Sjef Boeren ◽  
Jos A. Hageman ◽  
...  
Keyword(s):  
Breast Milk ◽  
Digestive System ◽  
Serum Proteins ◽  
Milk Serum
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