scholarly journals Hypocholesterolaemic Activity of Lupin Peptides: Investigation on the Crosstalk between Human Enterocytes and Hepatocytes Using a Co-Culture System Including Caco-2 and HepG2 Cells

Nutrients ◽  
2016 ◽  
Vol 8 (7) ◽  
pp. 437 ◽  
Author(s):  
Carmen Lammi ◽  
Chiara Zanoni ◽  
Simonetta Ferruzza ◽  
Giulia Ranaldi ◽  
Yula Sambuy ◽  
...  
Keyword(s):  
Hepg2 Cells ◽  
Culture System ◽  
Hypocholesterolaemic Activity

scholarly journals UPLC-MS/MS analysis of ochratoxin A metabolites produced by Caco-2 and HepG2 cells in a co-culture system

2017 ◽  
Vol 109 ◽  
pp. 333-340 ◽  
Author(s):  
Cyndia A. González-Arias ◽  
Sonia Marín ◽  
Aurora E. Rojas-García ◽  
Vicente Sanchis ◽  
Antonio J. Ramos
Keyword(s):  
Ochratoxin A ◽  
Hepg2 Cells ◽  
Culture System ◽  
Ms Analysis

scholarly journals Butyric Acid Added Apically to Intestinal Caco-2 Cells Elevates Hepatic ApoA-I Transcription and Rescues Lower ApoA-I Expression in Inflamed HepG2 Cells Co-Cultured in the Basolateral Compartment

Biomolecules ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 71
Author(s):  
Jehad Z. Tayyeb ◽  
Herman E. Popeijus ◽  
Ronald P. Mensink ◽  
Jogchum Plat
Keyword(s):  
Butyric Acid ◽  
Hepg2 Cells ◽  
Culture System ◽  
Density Lipoprotein ◽  
Short Chain Fatty Acids ◽  
Culture Experiment ◽  
Apolipoprotein A ◽  
Culture Model ◽  
Hdl Functionality ◽  
Anti Inflammatory

Apolipoprotein A-I (ApoA-I) concentrations are decreased during inflammation, which may reduce high-density lipoprotein (HDL) functionality. Thus, rescuing ApoA-I concentrations during inflammation might help to prevent atherosclerosis. Recent studies have shown that butyric acid (C4) has anti-inflammatory effects and rescues ApoA-I production. However, whether intestinal short chain fatty acids (SCFAs) are able to influence hepatic processes is unknown. Therefore, we investigated C4 anti-inflammatory effects on ApoA-I transcription in the intestine-liver co-culture model. C4 dose-response experiments in the presence or absence of cytokines were performed in a co-culture system including Caco-2 cells, HepG2 cells, or both. Changes in ApoA-I transcription in Caco-2 cells and HepG2 cells were analyzed using qPCR. C4 increased ApoA-I expression in HepG2 cells that cultured alone. When both cells were cultured together, C4 decreased ApoA-I expression in Caco-2 cells and increased ApoA-I expression in HepG2 cells. However, adding C4 to apical Caco-2 cells resulted in a smaller effect in HepG2 cells compared with adding C4 directly to the hepatocytes. Moreover, C4 rescued ApoA-I expression in inflamed HepG2 cells. These findings suggests that intestinal SCFAs can affect hepatic processes. However, the smaller effect in the co-culture experiment indicates cross-talk between intestine and liver.

scholarly journals In vitro biotransformation of ochratoxin A using a co-culture system with Caco-2 and HepG2 cells

2016 ◽  
Vol 259 ◽  
pp. S136
Author(s):  
C.A. González-Arias ◽  
S. Marín ◽  
A.E. Rojas-García ◽  
V. Sanchis ◽  
A.J. Ramos
Keyword(s):  
Ochratoxin A ◽  
Hepg2 Cells ◽  
Culture System

Effects of hydroponic culture system and NaCl on interactions between common bean lines and native rhizobia from Tunisian soils

Agronomie ◽  
2001 ◽  
Vol 21 (6-7) ◽  
pp. 601-605 ◽  
Author(s):  
Moez Jebara ◽  
Jean-Jacques Drevon ◽  
Mohamed Elarbi Aouani
Keyword(s):  
Common Bean ◽  
Culture System ◽  
Hydroponic Culture
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