scholarly journals Body Position Modulates Gastric Emptying and Affects the Post-Prandial Rise in Plasma Amino Acid Concentrations Following Protein Ingestion in Humans

Nutrients ◽  
2016 ◽  
Vol 8 (4) ◽  
pp. 221 ◽  
Author(s):  
Andrew Holwerda ◽  
Kaatje Lenaerts ◽  
Jörgen Bierau ◽  
Luc van Loon
Keyword(s):  
Amino Acid ◽  
Gastric Emptying ◽  
Body Position ◽  
Plasma Amino Acid ◽  
Protein Ingestion ◽  
Amino Acid Concentrations

scholarly journals The glycation level of milk protein strongly modulates post-prandial lysine availability in humans

2019 ◽  
Vol 123 (5) ◽  
pp. 545-552 ◽  
Author(s):  
Jean Nyakayiru ◽  
Glenn A. A. van Lieshout ◽  
Jorn Trommelen ◽  
Janneau van Kranenburg ◽  
Lex B. Verdijk ◽  
...  
Keyword(s):  
Amino Acid ◽  
Milk Protein ◽  
Milk Proteins ◽  
Ultra Performance Liquid Chromatography ◽  
Protein Glycation ◽  
Plasma Amino Acid ◽  
Amino Acid Availability ◽  
Protein Ingestion ◽  
The Impact ◽  
Amino Acid Concentrations

AbstractIndustrial heat treatment of milk results in protein glycation. A high protein glycation level has been suggested to compromise the post-prandial rise in plasma amino acid availability following protein ingestion. In the present study, we assessed the impact of glycation level of milk protein on post-prandial plasma amino acid responses in humans. Fifteen healthy, young men (age 26 (SEM 1) years, BMI 24 (SEM 1) kg/m2) participated in this randomised cross-over study and ingested milk protein powder with protein glycation levels of 3, 20 and 50 % blocked lysine. On each trial day, arterialised blood samples were collected at regular intervals during a 6-h post-prandial period to assess plasma amino acid concentrations using ultra-performance liquid chromatography. Plasma essential amino acid (EAA) concentrations increased following milk protein ingestion, with the 20 and 50 % glycated milk proteins showing lower overall EAA responses compared with the 3 % glycated milk protein (161 (SEM 7) and 142 (SEM 7) v. 178 (SEM 9) mmol/l × 6 h, respectively; P ≤ 0·011). The lower post-prandial plasma amino acid responses were fully attributed to an attenuated post-prandial rise in circulating plasma lysine concentrations. Plasma lysine responses (incremental AUC) following ingestion of the 20 and 50 % glycated milk proteins were 35 (SEM 4) and 92 (SEM 2) % lower compared with the 3 % glycated milk protein (21·3 (SEM 1·4) and 2·8 (SEM 0·7) v. 33·3 (SEM 1·7) mmol/l × 6 h, respectively; P < 0·001). Milk protein glycation lowers post-prandial plasma lysine availability in humans. The lower post-prandial availability of lysine following ingestion of proteins with a high glycation level may compromise the anabolic properties of a protein source.

scholarly journals Food ingestion in an upright sitting position increases postprandial amino acid availability when compared with food ingestion in a lying down position

2017 ◽  
Vol 42 (7) ◽  
pp. 738-743 ◽  
Author(s):  
Andrew M. Holwerda ◽  
Kaatje Lenaerts ◽  
Jörgen Bierau ◽  
Will K.W.H. Wodzig ◽  
Luc J.C. van Loon
Keyword(s):  
Amino Acid ◽  
Gastric Emptying ◽  
Dietary Protein ◽  
Muscle Protein ◽  
Peak Plasma ◽  
Body Position ◽  
Protein Digestion ◽  
Food Ingestion ◽  
Amino Acid Availability ◽  
Protein Ingestion

Dietary protein digestion and absorption kinetics determine the postprandial increase in muscle protein synthesis. We recently demonstrated that body position during feeding can modulate the postprandial rise in plasma amino acid availability. Here we investigated whether protein ingestion in an upright sitting body position accelerates gastric emptying and improves dietary protein digestion and subsequent amino acid absorption compared with feeding in a supine lying body position. In a crossover design, 8 young males (age, 26 ± 1 years; body mass index, 24.0 ± 0.9 kg·m−2) ingested 20 g intrinsically l-[1-13C]-phenylalanine–labeled milk protein plus 1.5 g paracetamol while sitting in an upright position or lying down in a supine position. Blood samples were collected frequently during a 5-h postprandial period. Gastric emptying rates and dietary protein digestion and absorption were assessed using plasma paracetamol and amino acid concentrations as well as plasma l-[1-13C]-phenylalanine enrichments. Peak plasma leucine concentrations were higher when protein was ingested in an upright sitting versus lying position (213 ± 15 vs 193 ± 12 μmol·L−1, P < 0.05), which was accompanied by a trend for a greater overall leucine response (13 989 ± 720 vs 11 875 ± 1073 AU, respectively; P = 0.05). Peak plasma paracetamol concentrations were higher in the sitting versus lying treatment (11.6 ± 0.5 vs 9.3 ± 0.6 mg·L−1, P < 0.05). Protein ingestion in an upright sitting position accelerates gastric emptying and increases the postprandial rise in plasma amino acid availability by increasing protein digestion and amino acid absorption rates. Therefore, feeding in an upright body position as opposed to a lying position is an important prerequisite to allow proper postprandial muscle protein accretion.

Non-union development related to pathologic callus and plasma amino acid concentrations

Injury ◽  
2009 ◽  
Vol 40 ◽  
pp. S20-S21
Author(s):  
K.A.P. Wijnands ◽  
Chc. Dejong ◽  
P.R.G. Brink ◽  
M. Poeze
Keyword(s):  
Amino Acid ◽  
Plasma Amino Acid ◽  
Non Union ◽  
Amino Acid Concentrations

scholarly journals Effect of Mixed Meal and Leucine Intake on Plasma Amino Acid Concentrations in Young Men

Nutrients ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 1543 ◽  
Author(s):  
Naomi Yoshii ◽  
Koji Sato ◽  
Riki Ogasawara ◽  
Yusuke Nishimura ◽  
Yasushi Shinohara ◽  
...  
Keyword(s):  
Amino Acid ◽  
Protein Intake ◽  
Young Men ◽  
Plasma Amino Acid ◽  
Maximum Plasma ◽  
Mixed Meal ◽  
Significant Difference ◽  
Plasma Leucine ◽  
Amino Acid Concentrations ◽  
Meal Meal

Dietary protein intake is critical for the maintenance of skeletal muscle mass. Plasma amino acid concentrations increase with protein intake and increases in muscle protein synthesis are dependent on leucine concentrations. We aimed to investigate the effect of a mixed meal and free amino acids intake on plasma leucine concentrations. In this randomized crossover study, 10 healthy young men (age 25 ± 1 years, height 1.73 ± 0.02 m, weight 65.8 ± 1.5 kg) underwent tests under different conditions—intake of 2 g of leucine (LEU), intake of a mixed meal (protein 27.5 g, including 2.15 g of leucine, protein: fat: carbohydrate ratio—22:25:53) only (MEAL), intake of 2 g of leucine immediately after a mixed meal (MEAL-LEU) and intake of 2 g of leucine 180 min after a mixed meal (MEAL-LEU180). Blood samples were collected within 420 min (240 min for LEU only) after intake and changes in amino acid concentrations were evaluated. Although the maximum plasma leucine concentration increased to 442 ± 24 µM for LEU, it was lower at 347 ± 16 µM (p < 0.05 vs. LEU) for MEAL-LEU, 205 ± 8 µM (p < 0.05 vs. LEU) for MEAL. The maximum plasma leucine concentration for MEAL-LEU180 increased to 481 ± 27 µM and compared to LEU there was no significant difference (p > 0.1). The observation that rapid elevations in plasma leucine concentrations are suppressed when leucine is ingested at the same time as a meal suggests that the timing of its intake must be considered to maximize the anabolic response.

Long-Term Voluntary Fasting in Adult Obese Cats: Nitrogen Balance, Plasma Amino Acid Concentrations and Urinary Orotic Acid Excretion

1994 ◽  
Vol 124 (suppl_12) ◽  
pp. 2680S-2682S ◽  
Author(s):  
Vincent Biourge ◽  
Joseph M. Groff ◽  
James G. Morris ◽  
Quinton R. Rogers
Keyword(s):  
Amino Acid ◽  
Nitrogen Balance ◽  
Orotic Acid ◽  
Plasma Amino Acid ◽  
Acid Excretion ◽  
Amino Acid Concentrations
Sign in / Sign up

Export Citation Format

Share Document