scholarly journals Protective Effects of Collagen Tripeptides in Human Aortic Endothelial Cells by Restoring ROS-Induced Transcriptional Repression

Nutrients ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 2226
Author(s):  
Hidehito Saito-Takatsuji ◽  
Yasuo Yoshitomi ◽  
Yasuhito Ishigaki ◽  
Shoko Yamamoto ◽  
Noriaki Numata ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Transcriptional Repression ◽  
Type I Collagen ◽  
Biological Effects ◽  
Osteoblastic Cell ◽  
Type I ◽  
Osteoblastic Cell Line ◽  
Aortic Endothelial Cells ◽  
Human Aortic Endothelial Cells ◽  
Aortic Endothelial

Collagen tripeptide (CTP) is defined as a functional food material derived from collagenase digests of type I collagen and contains a high concentration of tripeptides with a Gly-X-Y sequence. CTP has several biological effects, including the acceleration of fracture healing, ameliorating osteoarthritis, and improving dryness and photoaging of the skin. Recently, an antiatherosclerotic effect of CTP has been reported, although its molecular mechanism is yet to be determined. In this study, we examined the effects of CTP on primary cultured human aortic endothelial cells (HAECs) under oxidative stress, because oxidative endothelial dysfunction is a trigger of atherosclerosis. DNA microarray and RT-qPCR analyses showed that CTP treatment recovered the downregulated expression of several genes, including the interleukin-3 receptor subunit alpha (IL3RA), which were suppressed by reactive oxygen species (ROS) treatment in HAECs. Furthermore, IL3RA knockdown significantly decreased the viability of HAECs compared with control cells. RT-qPCR analysis also showed that solute carrier 15 family peptide transporters, which are involved in CTP absorption into cells, were expressed in HAECs at levels more than comparable to those of a CTP-responsive human osteoblastic cell line. These results indicated that CTP exerts a protective effect for HAECs, at least in part, by regulating the recovery of ROS-induced transcriptional repression.

Human aortic endothelial cells express the type I but not the type II receptor for interleukin-1 (IL-1)

1992 ◽  
Vol 153 (3) ◽  
pp. 583-588 ◽  
Author(s):  
Ann L. Akeson ◽  
Laura B. Mosher ◽  
Connie W. Woods ◽  
Kendra K. Schroeder ◽  
Terry L. Bowlin
Keyword(s):  
Endothelial Cells ◽  
Interleukin 1 ◽  
Type I ◽  
Type Ii ◽  
Aortic Endothelial Cells ◽  
Human Aortic Endothelial Cells ◽  
Aortic Endothelial

scholarly journals MDP Up-Regulates the Gene Expression of Type I Interferons in Human Aortic Endothelial Cells

Molecules ◽  
2012 ◽  
Vol 17 (4) ◽  
pp. 3599-3608 ◽  
Author(s):  
Qingshan Lv ◽  
Mei Yang ◽  
Xueting Liu ◽  
Lina Zhou ◽  
Zhilin Xiao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endothelial Cells ◽  
Type I Interferons ◽  
Type I ◽  
Aortic Endothelial Cells ◽  
Human Aortic Endothelial Cells ◽  
Aortic Endothelial

Organization of the cytoskeleton and the focal contacts of bovine aortic endothelial cells cultured on type I and III collagen.

1990 ◽  
Vol 38 (1) ◽  
pp. 59-67 ◽  
Author(s):  
R Semich ◽  
H Robenek
Keyword(s):  
Endothelial Cells ◽  
Type I Collagen ◽  
Type Iii Collagen ◽  
Type I ◽  
Aortic Endothelial Cells ◽  
Bovine Aortic Endothelial Cells ◽  
Focal Contacts ◽  
Cells Cultured ◽  
In Cells ◽  
Aortic Endothelial

We investigated the organization of the cytoskeleton and the focal contacts of bovine aortic endothelial cells cultured on type I and III collagen. The influence of these collagens on cell morphology and the distribution pattern of actin, vimentin, talin, and vinculin was analyzed by light microscopy, conventional electron microscopy, immunofluorescence, and immunogold labeling after lysis-squirting. Whereas the morphology of the endothelial cells is not markedly influenced, the structure of the cytoskeleton and the focal contacts of the cells are altered by the different collagen types. Stress fibers are more distinct in cells grown on type I collagen; cells on type III collagen show a more diffuse distribution of actin molecules. Intermediate filaments seem not to be affected by the collagens. The areas of focal contacts are larger in cells on type I collagen. Additionally, the labeling pattern of talin and vinculin is denser in focal contacts of cells grown on type I collagen. These results suggest an important role of the type of collagen in mediation of the organization of the microfilament system and the adhesion structures of bovine aortic endothelial cells in culture.

scholarly journals P773Uric acid regulates multiple interacting major cellular pathways in human aortic endothelial cells: a global proteome approach

2014 ◽  
Vol 103 (suppl 1) ◽  
pp. S142.1-S142
Author(s):  
A Oberbach ◽  
V Adams ◽  
N Schlichting ◽  
N Jehmich ◽  
U Voelker ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Aortic Endothelial Cells ◽  
Cellular Pathways ◽  
Human Aortic Endothelial Cells ◽  
Aortic Endothelial
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