scholarly journals The Functional Vision Protection Effect of Danshensu via Dopamine D1 Receptors: In Vivo Study

Nutrients ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 978
Author(s):  
Yun-Wen Chen ◽  
Yun-Ping Huang ◽  
Pei-Chang Wu ◽  
Wei-Yu Chiang ◽  
Ping-Hsun Wang ◽  
...  
Keyword(s):  
Protective Effect ◽  
Salvia Miltiorrhiza ◽  
High Energy ◽  
Müller Cell ◽  
D1 Receptors ◽  
Dopamine D1 Receptors ◽  
Visual Contrast ◽  
Functional Vision ◽  
Muller Cell

Danshensu, a traditional herb-based active component (Salvia miltiorrhiza Bunge), has garnered attention, due to its safety, nutritional value, and antioxidant effects, along with cardiovascular-protective and neuroprotective abilities; however, its effect on the retinal tissues and functional vision has not been fully studied. The objective of this study was to analyze the protective effect of danshensu on retinal tissues and functional vision in vivo in a mouse model of light-induced retinal degeneration. High energy light-evoked visual damage was confirmed by the loss in structural tissue integrity in the retina accompanied by a decline in visual acuity and visual contrast sensitivity function (VCSF), whereas the retina tissue exhibited severe Müller cell gliosis. Although danshensu treatment did not particularly reduce light-evoked damage to the photoreceptors, it significantly prevented Müller cell gliosis. Danshensu exerted protective effects against light-evoked deterioration on low spatial frequency-based VCSF as determined by the behavioral optomotor reflex method. Additionally, the protective effect of danshensu on VCSF can be reversed and blocked by the injection of a dopamine D1 receptor antagonist (SCH 23390). This study demonstrated that the major functional vision promotional effect of danshensu in vivo was through the dopamine D1 receptors enhancement pathway, rather than the structural protection of the retinas.

Müller cell gliosis in retinal organ culture mimics gliotic alterations after ischemia in vivo

2008 ◽  
Vol 26 (7) ◽  
pp. 745-751 ◽  
Author(s):  
Heidrun Kuhrt ◽  
Antje Wurm ◽  
Anett Karl ◽  
Ianors Iandiev ◽  
Peter Wiedemann ◽  
...  
Keyword(s):  
Organ Culture ◽  
Müller Cell ◽  
Muller Cell

[11C]A-69024: A potent and selective non-benzazepine radiotracer for in vivo studies of dopamine D1 receptors

1995 ◽  
Vol 22 (2) ◽  
pp. 221-226 ◽  
Author(s):  
Michael Kassiou ◽  
Ursula Scheffel ◽  
Hayden T. Ravert ◽  
William B. Mathews ◽  
John L. Musachio ◽  
...  
Keyword(s):  
D1 Receptors ◽  
In Vivo Studies ◽  
Dopamine D1 Receptors

[3H]A-69024: A non-benzazepine ligand for in vitro and in vivo studies of dopamine d1 receptors

Life Sciences ◽  
1995 ◽  
Vol 57 (23) ◽  
pp. PL367-PL372 ◽  
Author(s):  
Michael Kassiou ◽  
Ursula A. Scheffel ◽  
John L. Musachio ◽  
Marigo Stathis ◽  
Robert F. Dannals
Keyword(s):  
D1 Receptors ◽  
In Vivo Studies ◽  
Dopamine D1 Receptors

Dopamine D1 receptors in human parathyroid gland: In vitro and in vivo studies

Life Sciences ◽  
1983 ◽  
Vol 33 (8) ◽  
pp. 743-747 ◽  
Author(s):  
A.J. Cross ◽  
I.N. Ferrier ◽  
C.A. Bloxham
Keyword(s):  
Parathyroid Gland ◽  
D1 Receptors ◽  
In Vivo Studies ◽  
Dopamine D1 Receptors

670nm light treatment following retinal injury modulates Müller cell gliosis: Evidence from in vivo and in vitro stress models

2018 ◽  
Vol 169 ◽  
pp. 1-12 ◽  
Author(s):  
Yen-Zhen Lu ◽  
Nilisha Fernando ◽  
Riccardo Natoli ◽  
Michele Madigan ◽  
Krisztina Valter
Keyword(s):  
Light Treatment ◽  
Müller Cell ◽  
Retinal Injury ◽  
Muller Cell ◽  
Stress Models ◽  
In Vitro Stress

scholarly journals Blocking IL-17A Alleviates Diabetic Retinopathy in Rodents

2017 ◽  
Vol 41 (3) ◽  
pp. 960-972 ◽  
Author(s):  
Ao-Wang Qiu ◽  
Qing-Huai Liu ◽  
Jun-Ling Wang
Keyword(s):  
Diabetic Retinopathy ◽  
Intravitreal Injection ◽  
Cell Function ◽  
Autoimmune Diabetes ◽  
Müller Cell ◽  
Cell Dysfunction ◽  
Junction Protein ◽  
Neutralizing Monoclonal Antibody ◽  
Muller Cell

Background/Aims: Interleukin (IL)-17A, a proinflammatory cytokine, has been implicated in several autoimmune diseases. However, it is unclear whether IL-17A is involved in diabetic retinopathy (DR), one of the most serious complications of autoimmune diabetes. This study aimed to demonstrate that IL-17A exacerbates DR by affecting retinal Müller cell function. Methods: High glucose (HG)-treated rat Müller cell line (rMC-1) was exposed to IL-17A, anti-IL-17A-neutralizing monoclonal antibody (mAb) or/and anti-IL-17 receptor (R)A-neutralizing mAb for 24 h. For in vivo study, DR was induced by intraperitoneal injections of streptozotocin (STZ). DR model mice were treated with anti-IL-17A mAb or anti-IL-17RA mAb in the vitreous cavity. Mice that were prepared for retinal angiography were sacrificed two weeks after intravitreal injection, while the rest were sacrificed two days after intravitreal injection. Results: IL-17A production and IL-17RA expression were increased in both HG-treated rMC-1 and DR retina. HG induced rMC-1 activation and dysfunction, as determined by the increased GFAP, VEGF and glutamate levels as well as the downregulated GS and EAAT1 expression. IL-17A exacerbated the HG-induced rMC-1 functional disorders, whereas either anti-IL-17A mAb or anti-IL-17RA mAb alleviated the HG-induced rMC-1 disorders. Intravitreal injections with anti-IL-17A mAb or anti-IL-17RA mAb in DR model mice reduced Müller cell dysfunction, vascular leukostasis, vascular leakage, tight junction protein downregulation and ganglion cell apoptosis in the retina. Conclusions: IL-17A aggravates DR-like pathology at least partly by impairing retinal Müller cell function. Blocking IL-17A is a potential therapeutic strategy for DR.

Pharmacological evaluation of (+)-2-[123I]A-69024 A radioligand for in vivo studies of dopamine D1 receptors

Life Sciences ◽  
2001 ◽  
Vol 69 (6) ◽  
pp. 669-675 ◽  
Author(s):  
Michael Kassiou ◽  
Karine Mardon ◽  
Filomena Mattner ◽  
Andrew Katsifis ◽  
Branko Dikic
Keyword(s):  
D1 Receptors ◽  
In Vivo Studies ◽  
Dopamine D1 Receptors ◽  
Pharmacological Evaluation
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