scholarly journals Association between Serum Vitamin B12 and Global DNA Methylation in Colorectal Cancer Patients

Nutrients ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 3567 ◽  
Author(s):  
Hatim Boughanem ◽  
Pablo Hernandez-Alonso ◽  
Alberto Tinahones ◽  
Nancy Babio ◽  
Jordi Salas-Salvadó ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Dna Methylation ◽  
Vitamin B12 ◽  
Serum Vitamin ◽  
Global Dna Methylation ◽  
Inverse Association ◽  
Insulin Metabolism ◽  
High Vitamin ◽  
Line1 Methylation ◽  
Tumor Area

Vitamin B12 has been widely related to methionine metabolism, which is an essential component for biological methylation reactions, including DNA methylation. However, the relationship between vitamin B12 and DNA methylation is still controversial. In addition, there is increasing evidence for the association between vitamin B12 and the risk of colorectal cancer (CRC), although results of this association need to be assessed with caution. For this purpose, we hypothesized that serum vitamin B12 could be associated with global DNA methylation in the CRC context. To test this hypothesis, we studied the association between global DNA methylation through long interspersed nuclear element-1 (LINE1) in CRC patients under the 25th percentile of serum vitamin B12. We found that the high vitamin B12 group had low LINE1 methylation in both tumor area and peripheral blood mononuclear cells (PBMCs) than the low serum vitamin B12 group. LINE1 methylation levels were significantly lower in tumor area compared to the adjacent tumor-free area, only in the high vitamin B12 group. LINE1 methylation in visceral adipose tissue (VAT) and PBMCs were correlated with tumoral, inflammatory, and insulin metabolism markers. However, the interaction between LINE1 methylation and vitamin B12 levels was associated with neoadjuvant therapy in the regression analysis only in men, suggesting a beneficial relationship. In conclusion, our results reported an inverse association between DNA methylation and vitamin B12 in the CRC context, which suggests that vitamin B12 may be implicated in an epigenetic state or mediation in CRC.

scholarly journals Low vitamin B12 status is negatively associated with global DNA methylation in young Chinese women

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Jiang‐Hui Zhu ◽  
Ling Hao ◽  
Zhu Li ◽  
Eoin Quinlivan ◽  
David Maneval ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Vitamin B12 ◽  
Chinese Women ◽  
Global Dna Methylation ◽  
Negatively Associated

RELATIONSHIP OF MATERNAL VITAMIN B12 STATUS IN PREGNANCY WITH PRETERM BIRTH AND MATERNAL HEALTH RISKS

2020 ◽  
pp. 32-38
Author(s):  
VICTORIA LAZAROVA SPASOVA ◽  
LILIA IVANOVA KOLEVA ◽  
MARIETA ANTONOVA POPOVA ◽  
VALENTINA BOYANOVA PETKOVA ◽  
MILEN VENTZISLAVOV DIMITROV
Keyword(s):  
Preterm Birth ◽  
Vitamin B12 ◽  
Pregnant Women ◽  
Serum Vitamin ◽  
Vitamin B12 Deficiency ◽  
Inverse Association ◽  
Maternal Vitamin ◽  
B12 Deficiency ◽  
The Impact ◽  
In Pregnancy

Vitamin B12 is known to be vital for cell growth and population during pregnancy. This retrospective and prospective case−control study was aimed to disclose a health risk for pregnant women with vitamin B12 deficiency, as well as the one of the preterm birth. The main tasks set and performed in this research were as follows: to compare the obstetrics anamnesis between the women who gave birth on term and women who gave birth before term; to find the prevalence of vitamin B12 insufficiency in pregnancy; to determine its association with preterm birth and low birth weight; to examine its association with spontaneous abortions, and to investigate its relationship with obesity and hemoglobin levels in pregnant women. The conducted investigation involved 107 women who gave birth before the 37th week of gestation and 101 women who gave birth after the 37th week of gestation at the outpatient clinic of the University Hospital "Maichin Dom" in Bulgaria. Our study revealed a correlation between maternal vitamin B12 deficiency, overweight and low hemoglobin level. Our results showed no significant correlation between serum vitamin B12 level and the risk of preterm birth. However, we found an inverse association between vitamin B12 level and overweight before pregnancy and at the time of giving birth. As well there was confirmed the strong connection between meat consumption and vitamin B12 level. The paper emphasizes that the deficiency of the vitamin occurs most likely in the women with inadequate diets. Such a deficiency is actually confirmed to have serious health consequences for pregnant women and their offspring. Therefore further profound and numerous studies should be performed to properly assess the correlation between vitamin B12 and preterm birth, as well as to understand better the impact of vitamin B12 over pregnant women. Key words: vitamin B12, preterm birth, pregnancy, overweight, hemoglobin.

scholarly journals Association between serum 25-hydroxyvitamin D and global DNA methylation in visceral adipose tissue from colorectal cancer patients

BMC Cancer ◽  
2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Daniel Castellano-Castillo ◽  
Sonsoles Morcillo ◽  
Ana B. Crujeiras ◽  
Lidia Sánchez-Alcoholado ◽  
Mercedes Clemente-Postigo ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Dna Methylation ◽  
Adipose Tissue ◽  
Cancer Patients ◽  
Visceral Adipose Tissue ◽  
Global Dna Methylation ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D ◽  
Colorectal Cancer Patients ◽  
Visceral Adipose

scholarly journals Effects of Altered Maternal Folic Acid, Vitamin B12 and Docosahexaenoic Acid on Placental Global DNA Methylation Patterns in Wistar Rats

PLoS ONE ◽  
2011 ◽  
Vol 6 (3) ◽  
pp. e17706 ◽  
Author(s):  
Asmita Kulkarni ◽  
Kamini Dangat ◽  
Anvita Kale ◽  
Pratiksha Sable ◽  
Preeti Chavan-Gautam ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Folic Acid ◽  
Docosahexaenoic Acid ◽  
Vitamin B12 ◽  
Wistar Rats ◽  
Global Dna Methylation ◽  
Methylation Patterns

scholarly journals Influence of nutrients involved in one-carbon metabolism on DNA methylation in adults—a systematic review and meta-analysis

2020 ◽  
Vol 78 (8) ◽  
pp. 647-666 ◽  
Author(s):  
Sophia D Amenyah ◽  
Catherine F Hughes ◽  
Mary Ward ◽  
Samuel Rosborough ◽  
Jennifer Deane ◽  
...  
Keyword(s):  
Systematic Review ◽  
Dna Methylation ◽  
Functional Analysis ◽  
Vitamin B12 ◽  
Carbon Metabolism ◽  
Meta Analysis ◽  
Molecular Techniques ◽  
Population Characteristics ◽  
Global Dna Methylation ◽  
Inclusion Criteria

Abstract Context Aberrant DNA methylation is linked to various diseases. The supply of methyl groups for methylation reactions is mediated by S-adenosylmethionine, which depends on the availability of folate and related B vitamins. Objectives To investigate the influence of key nutrients involved in 1-carbon metabolism on DNA methylation in adults. Data sources Systematic literature searches were conducted in the Cochrane Library, Medline, Embase, Cumulative Index to Nursing and Allied Health Literature Plus, Scopus, and Web of Science databases. Studies that met the inclusion criteria and were published in English were included. Data extraction The first author, study design, sample size, population characteristics, type and duration of intervention, tissue type or cells analyzed, molecular techniques, and DNA methylation outcomes. Data synthesis A meta-analysis of randomized, controlled trials (RCTs) was conducted to investigate the effect of 1-carbon metabolism nutrients on global DNA methylation. Functional analysis and visualization were performed using BioVenn software. Results From a total of 2620 papers screened by title, 53 studies met the inclusion criteria. Qualitative analysis indicated significant associations between 1-carbon metabolism nutrients and DNA methylation. In meta-analysis of RCTs stratified by method of laboratory analysis, supplementation with folic acid alone or in combination with vitamin B12 significantly increased global DNA methylation in studies using liquid chromatography–mass spectrometry, which had markedly lower heterogeneity (n = 3; Z = 3.31; P = 0.0009; I2 = 0%) in comparison to other methods. Functional analysis highlighted a subset of 12 differentially methylated regions that were significantly related to folate and vitamin B12 biomarkers. Conclusion This study supports significant associations between 1-carbon metabolism nutrients and DNA methylation. However, standardization of DNA methylation techniques is recommended to reduce heterogeneity and facilitate comparison across studies. Systematic Review registration PROSPERO registration number: CRD42018091898.

scholarly journals Inverse Association Between Serum Vitamin B12 Concentration and Obesity Among Adults in the United States

2019 ◽  
Vol 10 ◽  
Author(s):  
Yangbo Sun ◽  
Minxian Sun ◽  
Buyun Liu ◽  
Yang Du ◽  
Shuang Rong ◽  
...  
Keyword(s):  
United States ◽  
Vitamin B12 ◽  
Serum Vitamin ◽  
The United States ◽  
Inverse Association

scholarly journals Hiwi Promotes the Proliferation of Colorectal Cancer Cells via Upregulating Global DNA Methylation

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Lin Yang ◽  
Lei Bi ◽  
Qingwei Liu ◽  
Meng Zhao ◽  
Bin Cao ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Dna Methylation ◽  
Cell Lines ◽  
Adenovirus Vector ◽  
Global Dna Methylation ◽  
Cell Renewal ◽  
Western Blot Assay ◽  
Real Time Quantitative Pcr ◽  
Protein Levels ◽  
Ht 29

Hiwi is well known for its role in stem cell renewal, maintaining the resting stage, and downregulating cell cycle of stem cells via RNA silencing. And Hiwi overexpression has been recognized in several types of cancers. In the present study, we examined the Hiwi expression in colorectal cancer (CRC) specimens in both mRNA and protein levels via real-time quantitative PCR, western blot assay, and immunohistochemical staining. Then we explored the role of Hiwi in the cancer cell proliferation and in the DNA methylation in human CRC Caro-2 and HT-29 cell lines. Results demonstrated that both mRNA and protein levels of Hiwi were significantly higher in 38 CRC tissues than in 38 peritumor tissues. Moreover, the Hiwi overexpression with an adenovirus vector significantly promoted the proliferation of Caro-2 and HT-29 cells, associated with significant increase in the global DNA methylation levels. And the chemical inhibition of DNA methylation significantly restrained such proliferation promotion. In summary, we confirmed that Hiwi was overexpressed in CRC tissues and that the forced Hiwi overexpression promoted the proliferation and global DNA methylation of CRC cell lines. Our results imply for the first time that Hiwi promotes the proliferation of CRC cells via promoting global DNA methylation.

scholarly journals Association of Folate and Vitamins Involved in the 1-Carbon Cycle with Polymorphisms in the Methylenetetrahydrofolate Reductase Gene (MTHFR) and Global DNA Methylation in Patients with Colorectal Cancer

Nutrients ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1368 ◽  
Author(s):  
Ariana Ferrari ◽  
Giovana Tardin Torrezan ◽  
Dirce Maria Carraro ◽  
Samuel Aguiar Junior
Keyword(s):  
Colorectal Cancer ◽  
Dna Methylation ◽  
Carbon Cycle ◽  
Methylenetetrahydrofolate Reductase ◽  
Tumor Stage ◽  
Serum Folate ◽  
Global Dna Methylation ◽  
Mthfr Polymorphisms ◽  
Dietary Folate ◽  
Colon And Rectal Cancer

Folate, vitamin B2, vitamin B6, vitamin B12, choline, and betaine are nutrients involved in the 1-carbon cycle that can alter the levels of DNA methylation and influence genesis and/or tumor progression. Thus, the objective of this study was to evaluate the association of folate and vitamins involved in the 1-carbon cycle and MTHFR polymorphisms in global DNA methylation in patients with colorectal cancer gene. The study included 189 patients with colorectal adenocarcinoma answering a clinical evaluation questionnaire and the Food Frequency Questionnaire (FFQ) validated for patients with colon and rectal cancer. Blood samples were collected for evaluation of MTHFR gene polymorphisms in global DNA methylation in blood and in tumor. The values for serum folate were positively correlated with the equivalent total dietary folate (total DFE) (rho = 0.51, p = 0.03) and global DNA methylation (rho = 0.20, p = 0.03). Individuals aged over 61 years (p = 0.01) in clinicopathological staging III and IV (p = 0.01) and with + heterozygous mutated homozygous genotypes for the MTHFR A1298C gene had higher levels of global DNA methylation (p = 0.04). The association between dietary intake of folate, serum folate, and tumor stage were predictive of global DNA methylation in patients’ blood. The levels of serum folate, the dietary folate and the status of DNA methylation can influence clinicopathological staging.

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