scholarly journals Association between Serum Uric Acid Levels, Nutritional and Antioxidant Status in Patients on Hemodialysis

Nutrients ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2600 ◽  
Author(s):  
Etna Domínguez-Zambrano ◽  
José Pedraza-Chaverri ◽  
Ana Laura López-Santos ◽  
Omar Noel Medina-Campos ◽  
Cristino Cruz-Rivera ◽  
...  
Keyword(s):  
Uric Acid ◽  
Nutritional Status ◽  
Antioxidant Capacity ◽  
Antioxidant Status ◽  
Antioxidant Properties ◽  
Dialysis Session ◽  
Scavenging Activity ◽  
Protein Nitrogen ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Purpose: To determine the relationship between uric acid (UA) and nutritional and antioxidant status in hemodialysis (HD) patients, given that hyperuricemia could be an indicator of good nutritional status possibly because of the antioxidant properties of UA. Methods: Cross-sectional study with 93 patients on HD. Hyperuricemia was considered as UA ≥6.0 mg/dL in females and ≥7.0 mg/dL in males. Nutritional variables were registered. Blood samples were taken before the dialysis session to determine oxidative damage as plasma malondialdehyde (MDA) content, and antioxidant capacity measuring 2,2-diphenyl-piclrylhidrazil radical (DPPH●) scavenging activity and oxygen radical absorbance capacity (ORAC) value. Results: Patients with hyperuricemia had higher creatinine (11.9 vs. 10.5 mg/dL; p = 0.004), potassium (5.5 vs. 5.0 mg/dL; p = 0.014) levels; phase angle (5.8 vs. 4.9; p = 0.005), protein consumption (normalized protein nitrogen appearance, nPNA, 1.03 vs. 0.83; p = 0.013) than normouricemic patients. DPPH● scavenging activity was higher in hyperuricemic subjects (1.139 vs. 1.049 mM Trolox equivalents; p = 0.007); likewise, hyperuricemic subjects had less oxidant damage measured by MDA (10.6 vs. 12.7 nmol/mL; p = 0.020). Subjects with normouricemia were at higher risk of having a reactance to height (Xc/H) ratio less than 35 (OR 2.79; 95% CI, 1.1–7.017, p = 0.028); nPNA < 1.0 (OR 3.78; 95% CI, 1.4–10.2, p = 0.007), diagnosis of cachexia (OR 2.95; 95% CI, 1156–7.518, p = 0.021), potassium levels <5 (OR 2.97; 95% CI, 1.136–7.772, p = 0.023) and PA < 5.5° (OR 3.38; 95% CI, 1.309–8.749, p = 0.012.) Conclusions: Patients with hyperuricemia had higher antioxidant capacity and better nutritional status. Purines and protein restrictions in HD patients with hyperuricemia need to be reviewed individually for each patient. More studies are needed to stablish a cut point of UA levels in renal population.

scholarly journals Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry

Horticulturae ◽  
2021 ◽  
Vol 7 (11) ◽  
pp. 420
Author(s):  
Tijana Kolarević ◽  
Danijel D. Milinčić ◽  
Tatjana Vujović ◽  
Uroš M. Gašić ◽  
Ljiljana Prokić ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Properties ◽  
Callus Cultures ◽  
Total Phenolic ◽  
Ms Medium ◽  
Scavenging Activity ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Blueberry Leaves

The aim of this study was to evaluate the content and profile of the phenolic compounds (PCs) and antioxidant properties of field-grown leaves, in vitro leaves and in vitro callus cultures of the blackberry ‘Čačanska Bestrna’ and blueberry ‘Toro’. In vitro shoots of the selected genotypes were grown either on original Murashige and Skoog (MS) medium containing 1 mg/L BA, 0.1 mg/l IBA and 0.1 mg/l GA3 (‘Čačanska Bestrna’) or on MS medium with macroelements reduced to ½, 2 mg/L zeatin and 0.2 mg/L IAA (‘Toro’). Callus cultures were induced from in vitro leaves and established on MS medium with 2 mg/L BA and 2 mg/L 2,4-D (‘Čačanska Bestrna’) or MS medium with half strength macroelements, 2 mg/L BA, 2 mg/L 2,4-D and 1 mg/L NAA (‘Toro’). Total phenolic (TPC) and flavonoid content (TFC) were the highest in blueberry leaves, whereas low TPC and TFC values were obtained in callus cultures of both cultivars. A higher content of PCs in blueberry leaves compared to blackberry leaves was determined by the UHPLC-DAD MS/MS technique. Quercetin derivatives and phenolic acids were the dominant PCs in the leaves of both berries, whereas gallocatechin was present in a significant amount in blueberry leaves. Callus cultures of both berries had a specific PC profile, with none detected in the leaves except quercetin-3-O-glucoside and quercetin-3-O-rutinoside. Blackberry leaves showed the best antioxidant properties as estimated by ferric reducing power (FRP), ABTS•+ and DPPH• scavenging activity assays. Callus cultures of both berries exhibited three to five times lower ABTS•+ and ten to seventeen times lower DPPH• scavenging activity compared to corresponding leaves. The analyzed leaves and callus cultures can be a good source of PCs with good antioxidant properties and specific phenolics, respectively, for applications in the food and pharmaceutical industries.

scholarly journals Antioxidant capacity of temulawak drink (Curcuma xanthorrhiza) with white crystal sugar cane, red crystal sugar cane, palm sugar, and arenga palm sugar

2017 ◽  
Vol 14 (2) ◽  
pp. 39-46
Author(s):  
SETIYA NING RUM S ◽  
KAWIJI KAWIJI ◽  
SETYANINGRUM ARIVIANI
Keyword(s):  
Antioxidant Capacity ◽  
Sugar Cane ◽  
Sensory Quality ◽  
Total Phenol ◽  
White Crystal ◽  
Scavenging Activity ◽  
Sugar Addition ◽  
Palm Sugar ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Rum SSN, Kawiji, Setyaningrum A. 2016. Antioxidant capacity of temulawak drink (Curcuma xanthorrhiza) with white crystal sugar cane, red crystal sugar cane, palm sugar, and arenga palm sugar. Biofarmasi 14: 39-46. The aim of this study was to determine the antioxidant capacity of temulawak extract in water solution; to determine the antioxidant capacity of white crystal sugar cane, red crystal sugar cane, palm sugar, and arenga palm sugar which commonly used in making temulawak drink; to determine the synergic effect of sugar addition to temulawak drink product; and also to determine the sensory quality (colour, taste, and flavour) of temulawak drink produced by parameters. This research used Completely Randomized Design (CAD) with two factors, concentration of temulawak extract (10, 20, and 30 gr/litre), and the kind of sugar added (white crystal sugar cane, red crystal sugar cane, palm sugar, and arenga palm sugar) with 50 g/litre concentration of addition, respectively. This research was studied the antioxidant activity (radical DPPH scavenging activity), total phenol, and sensory analysis (Multiple Comparison Test). Statistical analysis was performed using SPSS 17.0 (α=0,05). This study showed that the radical DPPH scavenging activity and total phenol were increase due to the increase of temulawak extract concentration. It might be due to water-soluble phenol compound like xanthorrizol extracted more largely. Radical DPPH scavenging activity and total phenol of sugars were significantly different which from the highest to the lowest palm sugar, arenga palm sugar (which usually used by people to make traditional health drink), red crystal sugar cane and white crystal sugarcane, respectively. Synergic effect of temulawak drink antioxidant capacity occured due to the sugar addition. The study also showed that sensory quality of produced temulawak drink with all treatments was not significantly different.

scholarly journals ANTIOXIDANT AND IMMUNOSTIMULATING ACTIVITY OF POLYSACCHARIDES EXTRACTED FROM CUBED-SNAILFISH (Liparis tessellatus Gilbert & Burke, 1912) EGGS

2015 ◽  
Vol 2 (1) ◽  
pp. 78
Author(s):  
Bernadeth F Ticar ◽  
Bernadeth T Ticar ◽  
Zuliyati Rohmah ◽  
Zuliyati Rohmah ◽  
Si-Hyang Park ◽  
...  
Keyword(s):  
Antioxidant Properties ◽  
Raw 264.7 Cells ◽  
Specific Cell ◽  
No Production ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Ags Cells ◽  
Concentration Dependent Manner ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

An optimized enzymatic extraction using protamexTM was established for polysaccharides from Liparis tesellatus eggs (PLE). Total sugars, sulfated glycosaminoglycans, uronic acids, and hexosamines content of PLE were 43.2%, 9.0%, 23.9%, and 1.73%, respectively. Superoxide radical (SOx*) scavenging activity and 2,2-diphenyl-1-picrylhydrazyl (DPPH*) scavenging activity of PLE was tested to proof its antioxidant properties. Results on SOx* scavenging activity revealed that PLE has antioxidant activity although it’s not surpass the activity of Ascorbic acid as standard. DPPH* scavenging activity showed that PLE has activity increasing at a concentration-dependent manner. However, at 1.25 mg/ml, the anti-DPPH activities of PLE lowered, possibly due to the pro-oxidant action of the sample. PLE also has effects on NO production of RAW 264.7 cells, suggestive of their possible immunostimulating potential in the human body. On human keratinocyte (HaCaT cell line), HT29 and AGS cells lines, PLE was proofed has no toxicity and showed proliferative effects. This effect showed that PLE could have potency as wound healing promoter. An inference can be made that, PLE has indispensable antioxidant potencies as well as cell proliferating activities on specific cell lines which are deemed necessary in the field of medicine and their utilization as functional food supplements. Keywords: Antioxidant, Fish eggs, Immunostimulator, Liparis tessellatus, Polysaccharides 

scholarly journals Lipid oxidation and antimicrobial activity of cooked beef patties as influenced by leaf extracts of “Cemba” (Albizia lebbeckoides [DC.] Benth)

2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Hajrawati Hajrawati ◽  
Henny Nuraini ◽  
Irma Isnafia Arief ◽  
Dondin Sajuthi
Keyword(s):  
Lipid Oxidation ◽  
Antioxidant Capacity ◽  
Cold Storage ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Beef Patties ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Cemba (Albizia lebbeckoides [DC.] Benth.) leaf extract (CLE) was evaluated for some physical properties, antioxidant and antimicrobial activities incorporated into beef patties during cold storage. Four Formulation employed were control, butylated hydroxytoluene (BHT) 0.01, CLE 0.5, and CLE 1% (w/w). The variables measured were proximate composition, cooking parameters, pH, aw, WHC, color, total phenolic content, antioxidant capacity, DPPH scavenging activity, TBARS value, and microbial total. The data were analyzed using ANOVA one factor for proximate and cooking parameters, and ANOVA with factorial 4x5 for pH, aw, WHC, color, total phenolic content, antioxidant capacity, DPPH scavenging activity, TBARS value, and microbial total and continued with Tukey test. The results of the study showed that the addition of the CLE did not affect the proximate composition and cooking parameters of the patties. The cooked beef patties with 1% CLE showed significantly lower (P<0.05) for TBARS value, pH, bacterial total (mesophilic and psychrophilic) compared to 0.5% CLE and controls. The total phenolic content, antioxidant capacity, scavenging activity of CLE 1 % were significantly higher (P<0.05) than 0.5% CLE and controls during the cold storage period (0, 7, 14, 21, and 28 days). Addition of both 0.5 and 1% CLE in cooked beef patty reduced bacteria total. The addition of 1% CLE had equivalent to BHT 0.01% effect in retarding lipid oxidation. In conclusion, the CLE 1% was effective to retard lipid oxidation and inhibit bacteria growth of cooked beef patties.

scholarly journals Presumptive Relationship between Oxidoreduction Potential and Both Antibacterial and Antioxidant Activities of Herbs and Spices: Oxidoreduction Potential as a Companion Tool for Measuring the Antioxidant Activity

2018 ◽  
Vol 47 (2) ◽  
pp. 506-514 ◽  
Author(s):  
Duried ALWAZEER ◽  
Sally DHAM
Keyword(s):  
Antioxidant Activity ◽  
Plant Extracts ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Scavenging Activity ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Oxidoreduction Potential ◽  
Herbs And Spices

Antibacterial and antioxidant activities form an essential component of the bioactive properties of plant products. The antibacterial and the antioxidant properties of three types of aqueous extracts of four herbs and spices were evaluated. While the total phenolic content, DPPH radical scavenging activity and oxidoreduction potential methods were used for the antioxidant properties determination, the inhibition diameters of extracts toward the growth of Staphylococcus warneri, Bacillus cereus, Enterobacter cloacae and Proteus vulgaris were evaluated. Although the phenolic content did not correlate with the antibacterial activity of plant extracts, it linearly correlated (R2 = 0.827) with DPPH scavenging activity of the studied plants following the order: sumac > ginger > rosemary > cinnamon. The oxidoreduction potential values linearly correlated with both the phenolic content (R2 ≈ 0.88) and DPPH scavenging activity values (R2 ≈ 0.96). The oxidoreduction potential could be proposed as a useful companion tool combined with other techniques when determining the antioxidant activity of plant extracts and food products is considered.

Bioactive Compounds and Antioxidant Capacity of Exotic Fruits and Commercial Frozen Pulps from Brazil

2008 ◽  
Vol 14 (3) ◽  
pp. 207-214 ◽  
Author(s):  
M.I. Genovese ◽  
M. Da Silva Pinto ◽  
A.E. De Souza Schmidt Gonçalves ◽  
F.M. Lajolo
Keyword(s):  
Vitamin C ◽  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Scavenging Activity ◽  
Total Phenols ◽  
Scavenging Capacity ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Exotic Fruits ◽  
Β Carotene

The objective of this work was to characterize exotic fruits (cambuci, araça-boi, camu-camu, jaracatia, araça) and commercial frozen pulps (araça, cambuci, umbu, coquinho, pana, native passion fruit, cagaita) from Brazil in relation to their bioactive compounds contents and antioxidant capacity. Camu-camu (Myrciaria dubia) presented the highest vitamin C and total phenolics contents (397 and 1797 mg/100 gf.w., respectively) and the highest DPPH• scavenging capacity. Coquinho (Butia capitata) also showed a significant vitamin C content (43 mg/100 gf.w.). Among the commercial frozen pulps, cagaita presented the higher DPPH scavenging activity and inhibition of β-carotene bleaching. A good correlation between total phenols and DPPH scavenging activity was found for fruits (r = 0.997) and commercial frozen pulps (r = 0.738). However, no correlation was found for total phenols and inhibition of β-carotene bleaching. Quercetin and kaempferol derivatives were the main flavonoids present in all samples and cyanidin derivatives were detected only in camu-camu. Camu-camu and araça (Psidium guineensis) showed the highest total ellagic acid contents (48 and 63.5 mg/100 gf.w.). All commercial frozen pulps presented lower contents of bioactive compounds and antioxidant capacity than their respective fruits. According to our results, camu-camu and araça might be sources of bioactive compounds.

scholarly journals Peptides of marine animals as a potential source of natural antioxidants

2017 ◽  
Vol 189 (2) ◽  
pp. 192-203
Author(s):  
Ekaterina P. Karaulova ◽  
Anna I. Chepkasova
Keyword(s):  
Molecular Weight ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Protein Hydrolysate ◽  
Antioxidant Properties ◽  
Low Molecular Weight ◽  
Scavenging Activity ◽  
Marine Animals ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Peptides extracted from tissues of 11 shellfish species ( Corbicula japonica, Mercenaria stimpsoni, Anadara broughtoni, Patinopecten yessoesis, Crenomytilus grayanus, Spisula sachalinensis, Maktra chinensis, Serripes groenlandicus, Glycymeris yessoensis, Callista brevisiphonata, Dosinia japonica ) are tested for their antioxidant properties in different in vitro conditions. Frozen muscles and viscera of the shellfish were minced and subjected to water extraction of proteins and to hydrolysis with Protamex 1.5 MG. The peptides antioxidant activity was evaluated by assessing of 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity on free radicals generated in oxidative systems. In the experiment, the high perfomance liquid chromatography (HPLC) system (Agilent Technologies 1260) included the frame TSKgel G 3000PWXL, the flow rate was 0.1 mL/min (0.1 N NaCI-20 mM Tris-HCI, pH 8.0), the detection was made under 280 nm. Molecular weight of the proteins was determined by comparison of their retention time with pure protein standards. The enzyme activity was high in conditions of pH in the range of 5.5-8.0, with the maximum under pH 7.0. Among 11 shellfish species, the highest antioxidant activity in water extract was observed for C. japonica , M. stimpsoni , C. brevisiphonata , G. yessoensis , and C. grayanus : 86.3, 68.7, 72.3, 90.2, and 67.5 mg of ascorbic acid/g, respectively. The DPPH radical scavenging activity increased with increasing of the hydrolysis degree. The 1-step hydrolysis with Protamex enhanced the DPPH scavenging activity for all samples, with the highest value for the protein hydrolysate of M. stimpsoni tissues (215 mg of ascorbic acid/g). Size of generated peptides is important for the antioxidant activity therefore molecular weight distribution of the peptides during the proteolysis was investigated using HPLC. Portion of the low molecular weight peptides (≤ 1 kDa) in the protein hydrolysate was increased in 4-14 % as compared with the starting protein. The antioxidant activity correlated positively with the number of low molecular weight peptides in protein hydrolysates.

scholarly journals Characterization of Antioxidant Peptides from Thai Traditional Semi-Dried Fermented Catfish

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 262
Author(s):  
Manat Chaijan ◽  
Tanutchaporn Rodsamai ◽  
Sawanya Charoenlappanit ◽  
Sittiruk Roytrakul ◽  
Atikorn Panya ◽  
...  
Keyword(s):  
Amino Acid ◽  
Heating Temperature ◽  
Clarias Gariepinus ◽  
Antioxidant Properties ◽  
In Vitro Digestion ◽  
Scavenging Activity ◽  
Antioxidant Peptides ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Herein, the antioxidant peptides from a Thai traditional semi-dried fermented farmed hybrid catfish (Clarias macrocephalus × Clarias gariepinus) catfish, Pla Duk Ra, were characterized. After extraction and deproteinization, Pla Duk Ra crude peptide extract (CPE) was fractioned using 2 connected Hitrap Sephadex-G25 columns, yielding two significant fractions, F1 with higher browning intensity (A420) and F2. CPE, F1, and F2 had different amino acid profiles, contents, and sequences evaluated by LC-MS/MS, which could be responsible for their antioxidant properties. F2 contained the highest numbers of hydrophobic amino acid (HBA) (47.45%) and aromatic amino acid (27.31%), followed by F1, and CPE. The peptides with 8–24 amino acid residues were detected in CPE and its fractions. In CPE, F1, and F2, there were 69, 68, and 85 peptides with varied HBA content, respectively. ARHSYGMLYCSCPPND (50% HBA), ALRKMGRK (37.5% HBA), and ANWMIPLM (87.5% HBA) were the most prevalent peptides found in CPE, F1, and F2. Overall, F2 was the most effective at inhibiting free radicals (DPPH● and ABTS●+) and reactive oxygen species (hydroxyl radical, singlet oxygen, and hydrogen peroxide), followed by F1 and CPE. The metal chelation of F1 was, however, superior to that of F2 and CPE. For the stability test, the effects of pH, heating temperature, and in vitro digestion on the DPPH● scavenging activity of F2 were investigated. The activity was boosted by lowering the pH and raising the heating temperature. In the gastrointestinal tract model system, however, roughly 50% of DPPH● scavenging activity reduced after digesting.

Characterization of non polar-polar solvent extracts from some tea plantation shade tree leaves with special reference to antioxidant and antibacterial activities

2020 ◽  
pp. 15-21
Author(s):  
Arindam Ghosh ◽  
Soumya Majumder ◽  
Sumedha Saha ◽  
Malay Bhattacharya
Keyword(s):  
Cardiac Glycosides ◽  
Polar Solvent ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Shade Trees ◽  
Crude Fibre ◽  
Shade Tree ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Beneficial properties of shade trees of tea plantations other than their medicinal properties have been extensively studied. This research was initiated to explore the properties of some shade trees with special emphasis on their antioxidant and antibacterial properties. Leaves from shade tree like Dalbergia sissoo (DS), Cassia siamea (CS), Derris robusta (DR), Leucaena leucocephala (LL), Acacia lenticularis (AL) and Melia azedarach (MA) were used for the study. Characterization of shade tree leaves by determination of moisture, crude fibre and ash content and tests of non polar – polar solvent extracts for steroid, tannins, cardiac glycosides and coumarin, free radical scavenging, ferric reducing power, NO scavenging activities, quantification of Flavonoids and antibacterial activity were conducted. The average moisture, crude fibre and ash percentage of shade tree plants were found to be 62.95, 11.28 and 1.86 respectively. Methanol, ethanol, acetone and ethyl acetate respectively proved to be the most potent solvent for various phytochemical extractions as it gave positive results for tests like tannin, steroid, cardiac glycosides and coumarin. AL (91.46%), DR (92.69%), LL (94.32%) and MA (93.34%) leaf extracts showed a high level of DPPH scavenging activity in their water extracts. In DS (88.11%) and CS (83.23%) maximum DPPH scavenging activity was observed in Diethyl ether and Methanol extracts respectively.  Acetone extracts were more active than the water extracts in exhibiting ferric reducing power and NO scavenging activity. Summation of the quantity revealed that DS showed maximum presence of flavonoids and acetone as most potential for isolation of flavonoids. The decreasing order of summative antibacterial activity was recorded in DS, followed by CS, DR, AL, MA and LL. Chloroform showed the highest summative inhibition zone followed by ethanol, ethyl acetate, diethyl ether, acetone, water,  hexane, benzene and methanol. The antioxidant and antibacterial potential of shade trees were established.

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