scholarly journals The Impact of Dietary Fucosylated Oligosaccharides and Glycoproteins of Human Milk on Infant Well-Being

Nutrients ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 1105 ◽  
Author(s):  
Magdalena Orczyk-Pawiłowicz ◽  
Jolanta Lis-Kuberka
Keyword(s):  
Human Milk ◽  
Infant Nutrition ◽  
Feeding Strategy ◽  
Well Being ◽  
Human Milk Oligosaccharides ◽  
Adhesive Properties ◽  
Milk Oligosaccharides ◽  
Wide Range ◽  
Carbohydrate Components ◽  
The Impact

Apart from optimal nutritional value, human milk is the feeding strategy to support the immature immunological system of developing newborns and infants. The most beneficial dietary carbohydrate components of breast milk are human milk oligosaccharides (HMOs) and glycoproteins (HMGs), involved in both specific and nonspecific immunity. Fucosylated oligosaccharides represent the largest fraction of human milk oligosaccharides, with the simplest and the most abundant being 2′-fucosyllactose (2′-FL). Fucosylated oligosaccharides, as well as glycans of glycoproteins, as beneficial dietary sugars, elicit anti-adhesive properties against fucose-dependent pathogens, and on the other hand are crucial for growth and metabolism of beneficial bacteria, and in this aspect participate in shaping a healthy microbiome. Well-documented secretor status related differences in the fucosylation profile of HMOs and HMGs may play a key but underestimated role in assessment of susceptibility to fucose-dependent pathogen infections, with a potential impact on applied clinical procedures. Nevertheless, due to genetic factors, about 20% of mothers do not provide their infants with beneficial dietary carbohydrates such as 2′-FL and other α1,2-fucosylated oligosaccharides and glycans of glycoproteins, despite breastfeeding them. The lack of such structures may have important implications for a wide range of aspects of infant well-being and healthcare. In light of the above, some artificial mixtures used in infant nutrition are supplemented with 2′-FL to more closely approximate the unique composition of maternal milk, including dietary-derived fucosylated oligosaccharides and glycoproteins.

scholarly journals New strategies for profiling and characterization of human milk oligosaccharides

Glycobiology ◽  
2020 ◽  
Vol 30 (10) ◽  
pp. 774-786 ◽  
Author(s):  
Sara Porfirio ◽  
Stephanie Archer-Hartmann ◽  
G Brett Moreau ◽  
Girija Ramakrishnan ◽  
Rashidul Haque ◽  
...  
Keyword(s):  
Breast Milk ◽  
Human Milk ◽  
Milk Composition ◽  
Human Breast Milk ◽  
Human Milk Oligosaccharides ◽  
Bacterial Strains ◽  
Milk Oligosaccharides ◽  
Vast Number ◽  
The Impact

Abstract Human breast milk is an incredibly rich and complex biofluid composed of proteins, lipids and complex carbohydrates, including a diverse repertoire of free human milk oligosaccharides (HMOs). Strikingly, HMOs are not digested by the infant but function as prebiotics for bacterial strains associated with numerous benefits. Considering the broad variety of beneficial effects of HMOs, and the vast number of factors that affect breast milk composition, the analysis of HMO diversity and complexity is of utmost relevance. Using human milk samples from a cohort of Bangladeshi mothers participating in a study on malnutrition and stunting in children, we have characterized breast milk oligosaccharide composition by means of permethylation followed by liquid chromatography coupled with high-resolution tandem mass spectrometry (LC-MS/MS) analysis. This approach identified over 100 different glycoforms and showed a wide diversity of milk composition, with a predominance of fucosylated and sialylated HMOs over nonmodified HMOs. We observed that these samples contain on average 80 HMOs, with the highest permethylated masses detected being >5000 mass units. Here we report an easily implemented method developed for the separation, characterization and relative quantitation of large arrays of HMOs, including higher molecular weight sialylated HMOs. Our ultimate goal is to create a simple, high-throughput method, which can be used for full characterization of sialylated and/or fucosylated HMOs. These results demonstrate how current analytical techniques can be applied to characterize human milk composition, providing new tools to help the scientific community shed new light on the impact of HMOs during infant development.

scholarly journals The Role of Two Human Milk Oligosaccharides, 2′-Fucosyllactose and Lacto-N-Neotetraose, in Infant Nutrition

2019 ◽  
Vol 22 (4) ◽  
pp. 330 ◽  
Author(s):  
Badriul Hegar ◽  
Yulianti Wibowo ◽  
Ray Wagiu Basrowi ◽  
Reza Gunadi Ranuh ◽  
Subianto Marto Sudarmo ◽  
...  
Keyword(s):  
Human Milk ◽  
Infant Nutrition ◽  
Human Milk Oligosaccharides ◽  
Milk Oligosaccharides

scholarly journals Perspective: Human Milk Oligosaccharides: Fuel for Childhood Obesity Prevention?

2019 ◽  
Author(s):  
Sarah E Maessen ◽  
José G B Derraik ◽  
Aristea Binia ◽  
Wayne S Cutfield
Keyword(s):  
Human Milk ◽  
Obesity Prevention ◽  
Gut Microbiome ◽  
Well Being ◽  
Human Milk Oligosaccharides ◽  
Milk Oligosaccharides ◽  
Microbiome Profile ◽  
Infant Gut Microbiome ◽  
Potential Link

ABSTRACT Obesity begins early but has lifelong consequences for health and well-being. Breastfeeding is thought to be preventive against obesity, but the extent and cause of this association are not well understood. Human milk oligosaccharides (HMOs) are abundant in human milk and not present in commercially available infant formula. These complex sugars are thought to contribute to the development of the infant gut microbiome and immune system. Recently, they have been investigated as a potential link between breastfeeding and lower obesity risk. So far, only a few human studies have examined HMO composition of human milk in association with the infant′s concurrent anthropometry or subsequent growth in infancy, with conflicting results. However, HMOs have been shown to modulate the gut microbiome profile by selectively promoting the growth of specific bacteria, such as bifidobacteria. Moreover, there are differences in the gut microbiome of lean and obese humans, and there is some evidence that the early composition of the gut microbiome can predict later obesity. Although it seems that HMOs might have a role in infant growth and adiposity, there is not enough consistent evidence to understand their potential role in obesity prevention. More data, particularly from large or longitudinal studies, are needed to clarify the functions of HMOs and other breast-milk components in determining long-term health.

scholarly journals Effects of Human Milk Oligosaccharides on the Adult Gut Microbiota and Barrier Function

Nutrients ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2808
Author(s):  
Tanja Šuligoj ◽  
Louise Kristine Vigsnæs ◽  
Pieter Van den Abbeele ◽  
Athanasia Apostolou ◽  
Katia Karalis ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Human Milk ◽  
Barrier Function ◽  
Distal Colon ◽  
Human Milk Oligosaccharides ◽  
Gut Barrier ◽  
Milk Oligosaccharides ◽  
Caco2 Cell ◽  
Gut Barrier Function ◽  
The Impact

Human milk oligosaccharides (HMOs) shape the gut microbiota in infants by selectively stimulating the growth of bifidobacteria. Here, we investigated the impact of HMOs on adult gut microbiota and gut barrier function using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®), Caco2 cell lines, and human intestinal gut organoid-on-chips. We showed that fermentation of 2’-O-fucosyllactose (2’FL), lacto-N-neotetraose (LNnT), and combinations thereof (MIX) led to an increase of bifidobacteria, accompanied by an increase of short chain fatty acid (SCFA), in particular butyrate with 2’FL. A significant reduction in paracellular permeability of FITC-dextran probe was observed using Caco2 cell monolayers with fermented 2’FL and MIX, which was accompanied by an increase in claudin-8 gene expression as shown by qPCR, and a reduction in IL-6 as determined by multiplex ELISA. Using gut-on-chips generated from human organoids derived from proximal, transverse, and distal colon biopsies (Colon Intestine-Chips), we showed that claudin-5 was significantly upregulated across all three gut-on-chips following treatment with fermented 2’FL under microfluidic conditions. Taken together, these data show that, in addition to their bifidogenic activity, HMOs have the capacity to modulate immune function and the gut barrier, supporting the potential of HMOs to provide health benefits in adults.

scholarly journals A novel class of human milk oligosaccharides based on 6-galactosyllactose and containing N-acetylglucosamine branches extended by oligogalactoses

2021 ◽  
Author(s):  
Franz-Georg Hanisch ◽  
Clemens Kunz
Keyword(s):  
Human Milk ◽  
Infant Nutrition ◽  
Maldi Mass Spectrometry ◽  
The Novel ◽  
Human Milk Oligosaccharides ◽  
Milk Oligosaccharides ◽  
New Class ◽  
Terminal Galactose ◽  
Branched Chains

Human milk oligosaccharides (HMOs) have attracted much attention in recent years not only as a prebiotic factor, but in particular as an essential component in infant nutrition related to their impact in innate immunity. The backbone structures of complex HMOs generally contain single or repetitive lacto-N-biose (type 1) or lactosamine (type 2) units in either linear or branched chains extending from a lactose core. While all known branched structures originate from 3,6-substitution of the lactosyl core galactose, we here describe a new class of HMOs that tentatively branch at terminal galactose of 6-galactosyllactose. Another novel feature of this class of HMOs was found in linear oligo-galactosyl chains linked to one of the N-acetylglucosamine (GlcNAc) branches. The novel structures exhibit general formulas with hexose vs. hexosamine contents of 5/2 to 8/2 and can be designated as high-galactose (HG)-HMOs. In addition, up to three fucosyl residues are linked to the octa- to dodecasaccharides, which were detected in two human milk samples from Lewis blood group defined donors. Structural analyses of methylated glycans and their alditols comprised MALDI mass spectrometry, ESI-(CID)MS and linkage analyses by GC-MS of the derived partially methylated alditol acetates. Enzymatic degradation by application of β1-3,4-specific galactosidase supported the presence of terminal galactose linked [beta]1-6 to one of the two GlcNAc branches.

scholarly journals Human Milk Oligosaccharides (HMOs) and Infant Microbiota: A Scoping Review

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1429
Author(s):  
Cristina Sánchez ◽  
Cristina Fente ◽  
Patricia Regal ◽  
Alexandre Lamas ◽  
María Paz Lorenzo
Keyword(s):  
Breast Milk ◽  
Human Milk ◽  
Mode Of Delivery ◽  
Geographic Location ◽  
Microbial Colonization ◽  
Human Milk Oligosaccharides ◽  
Maternal Factors ◽  
Milk Oligosaccharides ◽  
Breastfed Infants ◽  
The Impact

Human milk oligosaccharides (HMOs) are the third most abundant solid component of breast milk. However, the newborn cannot assimilate them as nutrients. They are recognized prebiotic agents (the first in the newborn diet) that stimulate the growth of beneficial microorganisms, mainly the genus Bifidobacterium, dominant in the gut of breastfed infants. The structures of the oligosaccharides vary mainly according to maternal genetics, but also other maternal factors such as parity and mode of delivery, age, diet, and nutritional status or even geographic location and seasonality cause different breast milk oligosaccharides profiles. Differences in the profiles of HMO have been linked to breast milk microbiota and gut microbial colonization of babies. Here, we provide a review of the scope of reports on associations between HMOs and the infant gut microbiota to assess the impact of HMO composition.

scholarly journals Associations between human milk oligosaccharides and growth in infancy and early childhood

2020 ◽  
Vol 111 (4) ◽  
pp. 769-778 ◽  
Author(s):  
Hanna Lagström ◽  
Samuli Rautava ◽  
Helena Ollila ◽  
Anne Kaljonen ◽  
Olli Turta ◽  
...  
Keyword(s):  
Human Milk ◽  
Causal Relation ◽  
Mode Of Delivery ◽  
Child Growth ◽  
Birth Cohort Study ◽  
Human Milk Oligosaccharides ◽  
Milk Oligosaccharides ◽  
Milk Samples ◽  
Prepregnancy Bmi ◽  
The Impact

ABSTRACT Background Breastfeeding modulates infant growth and protects against the development of obesity. However, whether or not maternal variation in human milk components, such as human milk oligosaccharides (HMOs), is associated with programming of child growth remains unknown. Objective Our objective was to determine the association between maternal HMO composition and child growth during the first 5 y of life. In addition, the association between maternal prepregnancy BMI and HMO composition was assessed. Methods Human milk samples from 802 mothers were obtained from a prospective population-based birth cohort study, Steps to healthy development of Children (STEPS), conducted in Turku, Finland. HMO composition in these milk samples was analyzed by HPLC. Child growth data from 3 mo to 5 y were collected from municipal well-baby clinics and linked to maternal HMO composition data to test for associations. Results Maternal HMO composition 3 mo after delivery was associated with height and weight during the first 5 y of life in children of secretor mothers. Specifically, HMO diversity and the concentration of lacto-N-neo-tetraose (LNnT) were inversely associated and that of 2′-fucosyllactose (2′FL) was directly associated with child height and weight z scores in a model adjusted for maternal prepregnancy BMI, mode of delivery, birthweight z score, sex, and time. Maternal prepregnancy BMI was associated with HMO composition. Conclusions The association between maternal HMO composition and childhood growth may imply a causal relation, which warrants additional testing in preclinical and clinical studies, especially since 2′FL and LNnT are among the HMOs now being added to infant formula. Furthermore, altered HMO composition may mediate the impact of maternal prepregnancy BMI on childhood obesity, which warrants further investigation to establish the cause-and-effect relation.

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