scholarly journals Effect of the Lactococcus Lactis 11/19-B1 Strain on Atopic Dermatitis in a Clinical Test and Mouse Model

Nutrients ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 763 ◽  
Author(s):  
Takato Suzuki ◽  
Kyoko Nishiyama ◽  
Koji Kawata ◽  
Kotaro Sugimoto ◽  
Masato Isome ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Mouse Model ◽  
Lactococcus Lactis ◽  
Skin Lesions ◽  
T Cell Subsets ◽  
Eosinophil Infiltration ◽  
Clinical Test ◽  
Host Immune System ◽  
Cervical Lymph Nodes ◽  
Ad Mouse Model

Some lactic acid bacteria (LAB) are known to improve atopic dermatitis (AD) through the regulation and stimulation of the host immune system. In this study, we found that ingestion of yogurt containing Lactococcus lactis 11/19-B1 strain (L. lactis 11/19-B1) daily for 8 weeks significantly improved the severity scoring of atopic dermatitis (SCORAD) system score from 38.8 ± 14.4 to 24.2 ± 12.0 in children suffering from AD. We tried to identify which LAB species among the five species contained in the test yogurt contributed to the improvement in AD pathology using an AD mouse model induced by repeated application of 1-fluoro-2, 4-dinitrobenzene (DNFB). AD-like skin lesions on the dorsal skin and ear were most improved by L. lactis 11/19-B1 intake among the five LAB species. In addition, analysis of CD4+ T cell subsets in Peyer’s patches (PPs) and cervical lymph nodes (CLNs) indicated that the intake of L. lactis 11/19-B1 generally suppressed all subsets related to inflammation, i.e., Th1, Th2 and Th17, instead of activating the suppressive system, Treg, in the AD mouse model. Histological observations showed ingestion of L. lactis 11/19-B1 significantly suppressed severe inflammatory findings, such as inflammatory cell filtration, epidermal erosion and eosinophil infiltration. These results suggest that the immunomodulatory effects of L. lactis 11/19-B1 contribute to improvements in AD pathology.

scholarly journals Schizonepeta Tenuifolia with Alpinia Oxyphylla Alleviates Atopic Dermatitis and Improves the Gut Microbiome in Nc/Nga Mice

Pharmaceutics ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 722
Author(s):  
Ting Zhang ◽  
Jingyi Qiu ◽  
Xuangao Wu ◽  
Shaokai Huang ◽  
Heng Yuan ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Gut Microbiome ◽  
Alpha Diversity ◽  
Skin Lesions ◽  
Eosinophil Infiltration ◽  
Weight Changes ◽  
Serum Ige ◽  
Alpinia Oxyphylla ◽  
Schizonepeta Tenuifolia ◽  
Fat Diets

Atopic dermatitis (AD) is a chronic inflammatory skin disease that may be related to gut microbes. Schizonepeta Tenuifolia Briquet (STB) and Alpinia Oxyphylla Miquel (AOM) has traditionally been used for anti-inflammatory activity. We evaluated the effects of STB, AOM and STB+AOM extracts on 2,4-dinitro-1-chlorobenzene (DNCB)-induced AD skin lesions in Nc/Nga mice and action mechanism was explored. AD lesions were induced in the dorsal skin of Nc/Nga mice by topical application of 1% followed by 0.2% DNCB. After DNCB was applied, the mice had topical applications of either 30% water, 0.01% dexamethasone, 30% STB, 30% AOM, 15% STB + 15% AOM extracts in butylene glycol (BG). Each group was also fed corresponding high-fat diets with 1% dextrin (AD-Con and AD-Positive), 1% STB (AD-STB), 1% AOM (AD-AOM) and 0.5% STB + 0.5% (AD-MIX). Normal-control mice had no DNCB application. The study evaluated the skin AD severity, scratching behavior and weight changes of AD mice for 5 weeks. Compared with AD-Con, AD-STB, AD-AOM and AD-MIX alleviated the clinical AD symptoms (erythema, pruritus, edema, erosion and lichenification and scratching behaviors), normalized immune chemistry (serum IgE concentration, mast cells and eosinophil infiltration), improved skin hyperplasia and enhanced the gut microbiome. AD-STB, AD-AOM, AD-MIX and AD-positive treatments inhibited cutaneous mRNA expression of TNF-α, IL-4 and IL-13 and serum IgE concentrations. AD-MIX most effectively reduced clinical AD symptoms and proinflammatory cytokines. AD-Positive also reduced them but serum GOT and GPT concentrations were abnormally high. AD-STB and AD-MIX increased the alpha-diversity of fecal bacteria and reduced the serum acetate concentration, compared to the AD-Con. In conclusion, the mixture of STB and AOM is effective for treating AD symptoms locally and systemically without adverse effects and are potential interventions for atopic dermatitis.

scholarly journals Isolation of maltol derivatives from Stellera chamaejasme and the anti-atopic properties of maltol on skin lesions in DNCB-stimulated mice

RSC Advances ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 2125-2132 ◽  
Author(s):  
Beom-Geun Jo ◽  
No-June Park ◽  
Su-Nam Kim ◽  
Jonghwan Jegal ◽  
Sangho Choi ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Mouse Model ◽  
Skin Lesions ◽  
Stellera Chamaejasme

The aim of this study was to isolate maltol derivatives from S. chamaejasme and to investigate the anti-atopic dermatitis (anti-AD) effect of maltol in a 2,4-dinitrochlorobenzene (DNCB)-sensitized mouse model of AD.

Changes in CD4+ T cell subsets (Th-1, Th-2) in skin lesions of patients with atopic dermatitis

1993 ◽  
Vol 6 (1) ◽  
pp. 67
Author(s):  
Takao Fujimura ◽  
Akira Fujioka ◽  
Yuko Hamada ◽  
Mikio Masuzawa ◽  
Shigeo Nishiyama
Keyword(s):  
Atopic Dermatitis ◽  
T Cell ◽  
Skin Lesions ◽  
T Cell Subsets ◽  
Cd4 T Cell ◽  
Th 1

scholarly journals Therapeutic Effect of Rumex japonicus Houtt. on DNCB-Induced Atopic Dermatitis-Like Skin Lesions in Balb/c Mice and Human Keratinocyte HaCaT Cells

Nutrients ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 573 ◽  
Author(s):  
Hye Yang ◽  
Hyunkyoung Lee ◽  
Jong-Hyun Kim ◽  
Il-Hwa Hong ◽  
Du Hwang ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Skin Inflammation ◽  
Topical Administration ◽  
Skin Lesions ◽  
Mitogen Activated Protein Kinase ◽  
Eosinophil Infiltration ◽  
Hacat Cells ◽  
Rumex Japonicus

Rumex japonicus Houtt. (RJ) is traditionally used in folk medicines to treat patients suffering from skin disease in Korea and other parts of East Asia. However, the beneficial effect of RJ extract on atopic dermatitis (AD) has not been thoroughly examined. Therefore, this study aimed to investigate the anti-inflammatory effects of RJ on AD in vitro and in vivo. Treatment with RJ inhibited the phosphorylation of mitogen-activated protein kinase (MAPK) as well as the activation of nuclear factor-kappa B (NF-κB) in tumor necrosis factor-α (TNF-α) stimulated in HaCaT cells. The five-week-old Balb/c mice were used as an AD-like mouse model by treating them with 1-chloro-2, 4-dinitrobenzene (DNCB). Topical administration of RJ to DNCB-treated mice significantly reduced clinical dermatitis severity, epidermal thickness, and decreased mast cell and eosinophil infiltration into skin and ear tissue. These results suggest that RJ inhibits the development of AD-like skin lesions by regulating the skin inflammation responses in HaCaT cells and Balb/c mice. Thus, RJ may be a potential therapeutic agent for AD.

scholarly journals Subcutaneous injection of recombinant heat shock protein 70 ameliorates atopic dermatitis skin lesions in a mouse model

2020 ◽  
Vol 36 (3) ◽  
pp. 186-195
Author(s):  
Jun‐Kai Kao ◽  
Tzu‐Fang Hsu ◽  
Ming‐Sheng Lee ◽  
Tzu‐Cheng Su ◽  
Cheng‐Han Lee ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Heat Shock ◽  
Mouse Model ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Subcutaneous Injection ◽  
Skin Lesions

scholarly journals Anti-Inflammatory and Immune Modulatory Effects of Synbio-Glucan in an Atopic Dermatitis Mouse Model

Nutrients ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 1090
Author(s):  
Yoon-Hwan Kim ◽  
Min Soo Kang ◽  
Tae Hyeong Kim ◽  
Yunho Jeong ◽  
Jin-Ok Ahn ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Treatment Group ◽  
Mouse Model ◽  
Topical Treatment ◽  
Dietary Treatment ◽  
Skin Lesions ◽  
Positive Control ◽  
Control Group ◽  
Significant Difference ◽  
Antibody Arrays

Many trials have been conducted to treat atopic dermatitis (AD), but these therapies are generally unsuccessful because of their insufficiency or side effects. This study examined the efficacy of β-glucan derived from oats with fermented probiotics (called Synbio-glucan) on an AD-induced mouse model. For the experiment, Nc/Nga mice were exposed to a house dust mite extract (HDM) to induce AD. The mice were placed in one of four groups: positive control group, Synbio-glucan topical treatment group, Synbio-glucan dietary treatment group, and Synbio-glucan topical + dietary treatment group. The experiment revealed no significant difference in the serum IgE concentration among the groups. Serum cytokine antibody arrays showed that genes related to the immune response were enriched. A significant difference in the skin lesion scores was observed between the groups. Compared to the control group tissue, skin lesions were alleviated in the Synbio-glucan topical treatment group and Synbio-glucan dietary treatment group. Interestingly, almost normal structures were observed within the skin lesions in the Synbio-glucan topical + dietary treatment group. Overall, the β-glucan extracted from oats and fermented probiotic mixture is effective in treating atopic dermatitis.

scholarly journals Huangbai Liniment Ameliorates Skin Inflammation in Atopic Dermatitis

2021 ◽  
Vol 12 ◽  
Author(s):  
Ting Zheng ◽  
Miao Fan ◽  
Yunbo Wei ◽  
Jinhong Feng ◽  
Pengcheng Zhou ◽  
...  
Keyword(s):  
Immune Response ◽  
Atopic Dermatitis ◽  
Mouse Model ◽  
Skin Diseases ◽  
Skin Inflammation ◽  
Local Skin ◽  
Immune Balance ◽  
Type 2 Immune Response ◽  
Ad Mouse Model

Atopic dermatitis (AD), also known as atopic eczema, is one of the most common skin diseases and is characterized by allergic skin inflammation, redness, and itchiness and is associated with a hyperactivated type 2 immune response. The leading causes of AD include an imbalance in the immune system, genetic predisposition, or environmental factors, making the development of effective pharmacotherapies complex. Steroids are widely used to treat AD; however, they provide limited efficacy in the long term and can lead to adverse effects. Thus, novel treatments that offer durable efficacy and fewer side effects are urgently needed. Here, we investigated the therapeutic potential of Huangbai Liniment (HB), a traditional Chinese medicine, using an experimental AD mouse model, following our clinical observations of AD patients. In both AD patient and the mouse disease model, HB significantly improved the disease condition. Specifically, patients who received HB treatment on local skin lesions (3–4 times/day) showed improved resolution of inflammation. Using the 1-Chloro-2,4-dinitrobenzene (DNCB)-induced AD model in BALB/c mice, we observed that HB profoundly alleviated severe skin inflammation and relieved the itching. The dermatopathological results showed markedly reversed skin inflammation with decreased epidermal thickness and overall cellularity. Correspondingly, HB treatment largely decreased the mRNA expression of proinflammatory cytokines, including IL-1β, TNF-α, IL-17, IL-4, and IL-13, associated with declined gene expression of IL-33, ST2, and GATA3, which are connected to the type 2 immune response. In addition, HB restored immune tolerance by promoting regulatory T (TREG) cells and inhibiting the generation of TH1, TH2, and TH17 cells in vitro and in the DNCB-induced AD mouse model. For the first time, we demonstrate that HB markedly mitigates skin inflammation in AD patients and the DNCB-induced AD mouse model by reinvigorating the T cell immune balance, shedding light on the future development and application of novel HB-based therapeutics for AD.

scholarly journals IL‑18 knockout alleviates atopic dermatitis‑like skin lesions induced by MC903 in a mouse model

2020 ◽  
Vol 46 (2) ◽  
pp. 880-888
Author(s):  
Jia‑Long Chen ◽  
Xue‑Li Niu ◽  
Ya‑Li Gao ◽  
Lei Ma ◽  
Xing‑Hua Gao ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Mouse Model ◽  
Skin Lesions

scholarly journals Gyogamdan, a Traditional Medicine Prescription, Ameliorated Dermal Inflammation and Hyperactive Behavior in an Atopic Dermatitis Mouse Model Exposed to Psychological Stress

2021 ◽  
Vol 2021 ◽  
pp. 1-18
Author(s):  
Ly Thi Huong Nguyen ◽  
Uy Thai Nguyen ◽  
Min-Jin Choi ◽  
Tae-Woo Oh ◽  
In-Jun Yang ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Mouse Model ◽  
Traditional Medicine ◽  
Psychological Stress ◽  
Ethanolic Extract ◽  
Eosinophil Infiltration ◽  
Bv2 Cells ◽  
Tnf Α ◽  
Medicine Prescription

Psychological stress (PS) plays a significant role as an aggravating factor in atopic dermatitis (AD). The traditional medicine prescription, Gyogamdan, has been used to treat chest discomfort and mood disorders caused by PS. This study investigated the effects of an ethanolic extract of Gyogamdan (GGDE) on stress-associated AD models and the underlying mechanisms. 2,4-Dinitrochlorobenzene- (DNCB-) treated BALB/c mice were exposed to social isolation (SI) stress. The effects of orally administered GGDE (100 or 500 mg/kg) were evaluated by ELISA, western blotting, and an open field test (OFT). SI stress exaggerated the skin inflammation and induced locomotor hyperactivity in the AD mouse model. GGDE reduced the levels of IgE, TNF-α, IL-13, eotaxin, and VEGF and mast cell/eosinophil infiltration and prevented the decreases in the levels of involucrin and loricrin in the skin. GGDE also suppressed the SI-induced increases in corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH), and corticosterone (CORT) in socially isolated AD mice. Furthermore, GGDE reduced traveling distances and mean speed significantly in the OFT. The in vitro experiments were performed using HaCaT, HMC-1, PC12, and BV2 cells. In the TNF-α/IFN-γ- (TI-) stimulated HaCaT cells, GGDE decreased the thymus and activation-regulated chemokine (TARC) and macrophage-derived chemokine (MDC) production significantly by inhibiting p-STAT1 and NF-κB signaling. GGDE also reduced VEGF production in HMC-1 cells stimulated with CRH/substance P (SP) by inhibiting p-ERK signaling pathway. GGDE increased the cell viability significantly and suppressed apoptosis in CORT-stimulated PC12 cells. Moreover, GGDE suppressed the LPS-induced production of NO, TNF-α, IL-1β, and IL-6 in BV2 cells. These results suggest that GGDE might be useful in patients with AD, which is exacerbated by PS.

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