scholarly journals Net Absorption and Metabolism of β-Hydroxy- β-Methyl Butyrate during Late Gestation in a Pig Model

Nutrients ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 561
Author(s):  
Liang Hu ◽  
Niels Bastian Kristensen ◽  
Uffe Krogh ◽  
Peter Kappel Theil
Keyword(s):  
Amino Acids ◽  
Control Diet ◽  
Plasma Concentrations ◽  
Daily Intake ◽  
Late Gestation ◽  
Plasma Metabolites ◽  
Acid Oxidation ◽  
Indwelling Catheters ◽  
Methyl Butyrate ◽  
Arterial Plasma

The leucine metabolite, β-hydroxy-β-methyl butyrate (HMB), is widely used in human nutrition and animal production as a nutritional supplement. Although the HMB usage during late gestation has been demonstrated to have a positive effect on fetal development, knowledge on net absorption and metabolism of HMB and impact of HMB on branched chain amino acids (BCAAs) metabolism is lacking. To address this, we conducted a study using pigs during the perinatal period as a model organism. Eight-second parity sows were fitted with indwelling catheters in the femoral artery and in the portal, hepatic, femoral, and mesenteric veins. Eight hourly sets of blood samples were taken starting 30 min before the morning meal on day –10 and day –3 relative to parturition. Four control (CON) sows were fed a standard lactation diet from day –15 and throughout the experiment, and 4 HMB sows were fed the control diet supplemented with 15 mg Ca(HMB)2/kg body weight mixed in one third of the morning meal from day –10 until parturition. Blood gases, plasma metabolites, milk compositions, and apparent total tract digestibility of nutrients were measured. Arterial plasma concentrations of HMB (p < 0.001), Cys (p < 0.001), and Lys (p < 0.10) were increased in HMB supplemented sows, while arterial plasma triglycerides concentration was decreased (p < 0.05). The net portal recovery of Ala and Asp were increased in HMB sows (p < 0.05). Sows fed HMB had increased hepatic vein flow and net hepatic fluxes of Met, Asn, and Gln (p < 0.05). In contrast, the femoral extraction rates of Ala and Ser were decreased by dietary HMB supplementation (p < 0.05). Dietary HMB treatment and sampling time relative to feeding had an interaction on arterial concentrations, net portal fluxes, and femoral extraction rates of BCAAs. The net portal recovery of HMB was 88%, while 14% of supplemented HMB was excreted through urine and 4% through feces. Moreover, the gastrointestinal tract metabolized 8% while the liver metabolized 12%. Finally, 26% of the daily intake of HMB was secreted via colostrum at the day of farrowing. This study demonstrated that dietary HMB supplementation increased net uptake of amino acids and increased fatty acid oxidation through improving blood flow and insulin sensitivity during the late gestation. Most importantly, oral HMB administration could maintain a stable postprandial absorption and altered metabolism in BCAAs. Net portal flux of HMB at 5.5 to 6.5 h after feeding approached zero, indicating that HMB ideally should be administrated two or three times, daily.

scholarly journals Time-Restricted Feeding a High-fat Diet in Mice Elevates Plasma Concentration of Saturated Fatty Acids but Reduces Concentrations of Multiple Amino Acids (OR27-02-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Bret Rust ◽  
Aaron Mehus ◽  
Joseph Idso ◽  
Matthew Picklo
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Energy Expenditure ◽  
Control Diet ◽  
Saturated Fatty Acids ◽  
Plasma Metabolites ◽  
Dark Phase ◽  
Restricted Feeding ◽  
High Fat ◽  
Branched Chain

Abstract Objectives Obesity and obesity-related disease contribute to health care costs and pose serious health risks. Rodent studies indicate that time-restricted feeding (TRF) may be effective in reducing adiposity and metabolic disease associated with obesity. However, the metabolic pathways impacted by TRF in the context of obesogenic, high-fat (HF) diets need clarity. In the present work we examined the metabolomic changes in plasma induced by TRF of a HF diet in mice compared to a HF diet eaten ad libitum (AL) vs AL intake of a low-fat (LF) control diet. Methods Male mice (12 weeks old) were fed a LF-AL diet (16%en fat), a HF-AL diet (48%en fat) or a HF diet restricted to feeding for 12 hours per day during the dark phase (HF-TRF). In week 9 of the study, energy expenditure data were collected. After 12 weeks, animals were fasted and plasma collected for clinical chemistries and metabolomic analysis. Multivariate analysis was used to discriminate diet treatments in untargeted metabolomic data. Results Energy expenditure measurements throughout the day showed a markedly reduced fasting respiratory exchange ratio (RER) in HF-TRF mice during the inactive (light) phase compared to AL groups. Measures of insulin resistance, while increased with HF-AL intake, were resolved in the HF-TRF group. Partial least squares discriminant analysis revealed plasma non-esterified fatty acids (NEFA) and amino acids (AA) to be important discriminators between diet treatments. TRF resulted in elevated NEFA concentrations of the saturated fatty acids (12:0 to 18:0) and the polyunsaturated fatty acids α-linolenic acid and linoleic acid compared to HF-AL. Conversely, the concentrations of aromatic and branched chain amino acids were reduced in HF-TRF mice compared to HF-AL mice. Conclusions Alterations in plasma metabolites following TRF of a HF diet are consistent with greater lipid utilization during the inactive phase as reflected in the RER. Decreases in the aromatic and branched chain amino acid concentrations are consistent with improved insulin sensitivity in humans. Funding Sources This work was supported by USDA-ARS project 3062-51000-053-00D. Supporting Tables, Images and/or Graphs

scholarly journals Feeding Agroindustrial Byproducts to Light Lambs: Influence on Growth Performance, Diet Digestibility, Nitrogen Balance, Ruminal Fermentation, and Plasma Metabolites

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 600 ◽  
Author(s):  
Trinidad de Evan ◽  
Almudena Cabezas ◽  
Jesús de la Fuente ◽  
María Dolores Carro
Keyword(s):  
Growth Performance ◽  
Control Diet ◽  
Animal Health ◽  
Average Daily Gain ◽  
Plasma Concentrations ◽  
Barley Straw ◽  
Plasma Metabolites ◽  
Ruminal Fermentation ◽  
Feed Conversion ◽  
Homogeneous Groups

The objective of this study was to evaluate the effect of replacing cereals and protein concentrates in a high-cereal concentrate (control) for light lambs with corn distiller’s dried grains with solubles (DDGS; 18%), dried citrus pulp (DCP; 18%), and exhausted olive cake (EOC; 8%) in a byproduct (BYP) concentrate on growth performance, digestibility, ruminal fermentation, and plasma metabolites. Two homogeneous groups of Lacaune lambs (13.8 kg ± 0.25 kg) were fed one of each concentrates and barley straw ad libitum until reaching about 26 kg body weight. There were no differences between groups on feed intake, average daily gain, or feed conversion ratio, but the control diet had greater (p < 0.001) dry matter digestibility. Diet had no effect on post-mortem ruminal pH and total volatile fatty acid concentrations and profile, but NH3-N concentrations were lower (p = 0.003) for the BYP-fed group compared with the control one. However, plasma concentrations of amino acids, total proteins, urea, and hepatic enzymes were not affected by the diet. In conclusion, 44% of feed ingredients in the concentrate for light lambs can be replaced with a mixture of corn DDGS, DCP, and EOC without negatively affecting growing performance and animal health.

PSX-A-17 Late-Breaking: Methionine and total sulfur amino acid requirements for pups (>14 wk to 9 months), adults, and senior Labrador Retrievers using the indicator amino acid oxidation (IAAO) technique

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 374-374
Author(s):  
Jessica L Varney ◽  
Charlene Watson ◽  
Nicole Colopy ◽  
John Moss ◽  
Jordan T Weil ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Control Diet ◽  
Age Groups ◽  
Isotope Ratio Mass Spectrometry ◽  
Co2 Production ◽  
Acid Oxidation ◽  
Total Sulfur ◽  
Amino Acid Oxidation ◽  
Labrador Retrievers

Abstract Methionine and cystine are often considered limiting amino acids in canine diets but limited requirement studies have been conducted especially for different life stages. Eighteen Labrador Retrievers (6 pups (&gt;14 wk-9 month), 6 adults, and 6 seniors [&gt;8yr)] were utilized in feeding studies to evaluate the changing requirements of methionine (Met) and total sulfur amino acids (TSAA) as canines age. For this study, the indicator amino acid oxidation (IAAO) technique was utilized to determine the amino acid (AA) requirements in each of the three age groups. Dogs were subjected to diets ranging from deficient to excess, with indispensable amino acids formulated at 1.6x NRC values. To allow for adaptation, a control diet with same dietary ingredients, protein and amino acid levels was fed for two days prior to feeding the test diets on the third day. On test day, a baseline breath sample was collected for determining CO2 production using a respiration mask (Oxymax, Columbus Instruments). A priming dose of L-[1-13C] phenylalanine (Cambridge Isotope Laboratories, Inc.) based on body weight was utilized, followed by [1-13C] Phe doses every 30 minutes, spanning a four hour period. After each dose 13CO2 was collected, and enrichment was determined by isotope ratio mass spectrometry (IRMS). Results for IRMS were converted to atom percent excess (APE) and analyzed using a piecewise model of best fit (JMP® Pro 16). A segmented line regression showed Met and TSAA mean and population requirements for pups (&gt;14 wk-9 mo.) were 0.78 ± 0.16 and 1.53 ± 0.21 g/1000kcal (mean ± 2SD), respectively. Meanwhile, for adults, mean and population requirements for Met and TSAA were estimated to be 0.68 ± 0.19 and 1.4 ± 0.30 g/1000kcal (mean ± 2SD), respectively, and for seniors, Met and TSAA mean and population requirements were determined to be 0.62 ± 0.17 and 1.27 ± 0.23 g/1000kcal (mean ± 2SD), respectively.

Corticotropin-releasing factor in the ovine fetus and pregnant ewe: role of placenta

1991 ◽  
Vol 261 (4) ◽  
pp. R995-R1002 ◽  
Author(s):  
M. Keller-Wood ◽  
C. E. Wood
Keyword(s):  
Adrenocorticotropic Hormone ◽  
Plasma Concentrations ◽  
Late Pregnancy ◽  
Maternal Plasma ◽  
Corticotropin Releasing Factor ◽  
Late Gestation ◽  
Fetal Plasma ◽  
Ovine Fetus ◽  
Arterial Plasma ◽  
Pregnant Ewe

In the sheep, maternal plasma adrenocorticotropic hormone and cortisol are increased in late pregnancy, and fetal plasma cortisol and adrenocorticotropic hormone rise precipitously in late gestation. To test whether the ovine placenta secretes corticotropin-releasing factor (CRF) into either the maternal or fetal circulation, pregnant ewes and their fetuses were prepared with femoral arterial catheters and uterine and umbilical venous catheters. Samples were taken from all sites before and during hypoxia. There was no difference in CRF concentration across the placenta in the mothers or the fetuses under resting or hypoxemic conditions, but maternal and fetal arterial plasma CRF concentrations increased between 128 and 145 days. In a second study, maternal and fetal femoral venous plasma CRF concentrations were measured 1-19 days before spontaneous parturition. The mean concentration increased 8.6 +/- 0.6 pg/ml 11-19 days before parturition to 13.0 +/- 1.0 and 13.2 +/- 1.4 pg/ml in fetuses 4-8 and 1-3 days before parturition, respectively. Maternal plasma concentrations did not significantly increase in the days closer to parturition. These studies demonstrate that there are low but measurable CRF concentrations in fetal and maternal sheep plasma but that these are not the result of tonic placental secretion of CRF.

Effect of soybean roasting and monensin on microbial protein synthesis, ruminal parameters and plasma metabolites of lactating dairy cows

2015 ◽  
Vol 55 (5) ◽  
pp. 625
Author(s):  
E. Abdi ◽  
F. Fatahnia ◽  
M. Dehghan Banadaki ◽  
A. Azarfar ◽  
S. G. Mosavi
Keyword(s):  
Plasma Concentration ◽  
Dairy Cows ◽  
Volatile Fatty Acids ◽  
Control Diet ◽  
Plasma Concentrations ◽  
Soybean Seed ◽  
Plasma Metabolites ◽  
Microbial Protein ◽  
Lactating Dairy Cows ◽  
Dietary Treatments

This study was conducted to evaluate the effects of inclusion of roasted whole soybean seed and monensin (MO) in the diets of lactating dairy cows on plasma metabolites, ruminal parameters, and microbial protein synthesised in the rumen. Four multiparous Holstein lactating dairy cows (third parity; 656 ± 55 kg of liveweight; 83 ± 10 days in milk; 35 ± 4 kg/day milk yield) were assigned to a balanced 4 × 4 Latin square design. Each experimental period lasted 21 days with 14 days of treatment adaptation and 7 days of data collection. The control diet (C) was a total mixed ration consisting of 40% forage and 60% concentrate mixture on a dry matter (DM) basis. These cows were randomly assigned to one of the four dietary treatments. The first treatment was the C diet of unprocessed whole soybean seed, second was the C diet supplemented with 24 mg of MO/kg of DM (M), the third was roasted whole soybean seed (R) and the fourth treatment was R diet supplemented with 24 mg of MO/kg of DM (RM). Urinary excretion of creatinine and purine derivatives, microbial protein synthesised in the rumen, rumen pH and rumen concentrations of volatile fatty acids and ammonia were similar among the dietary treatments (P > 0.05). Orthogonal contrasts showed that the rumen concentration of acetate was lower in MO-supplemented cows than non-supplemented cows (P < 0.05). Dietary treatments had no effects on plasma concentrations of glucose, total cholesterol, triglycerides and total protein (P > 0.05). Plasma concentration of urea was significantly lower in cows fed with the RM diet compared with cows fed the C and M diets (P < 0.05). In conclusion, dietary treatments had no effect on microbial protein synthesised in the rumen, plasma metabolites (except for plasma concentration of urea) and ruminal parameters of dairy cows.

scholarly journals Prolonged maternal amino acid infusion in late-gestation pregnant sheep increases fetal amino acid oxidation

2009 ◽  
Vol 297 (3) ◽  
pp. E638-E646 ◽  
Author(s):  
Paul J. Rozance ◽  
Michelle M. Crispo ◽  
James S. Barry ◽  
Meghan C. O'Meara ◽  
Mackenzie S. Frost ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Supplementation ◽  
Late Gestation ◽  
Acid Oxidation ◽  
Leucine Incorporation ◽  
Oxygen Utilization ◽  
Amino Acid Oxidation ◽  
Amino Acid Infusion ◽  
Pregnant Sheep

Protein supplementation during human pregnancy does not improve fetal growth and may increase small-for-gestational-age birth rates and mortality. To define possible mechanisms, sheep with twin pregnancies were infused with amino acids (AA group, n = 7) or saline (C group, n = 4) for 4 days during late gestation. In the AA group, fetal plasma leucine, isoleucine, valine, and lysine concentrations were increased ( P < 0.05), and threonine was decreased ( P < 0.05). In the AA group, fetal arterial pH (7.365 ± 0.007 day 0 vs. 7.336 ± 0.012 day 4, P < 0.005), hemoglobin-oxygen saturation (46.2 ± 2.6 vs. 37.8 ± 3.6%, P < 0.005), and total oxygen content (3.17 ± 0.17 vs. 2.49 ± 0.20 mmol/l, P < 0.0001) were decreased on day 4 compared with day 0. Fetal leucine disposal did not change (9.22 ± 0.73 vs. 8.09 ± 0.63 μmol·min−1·kg−1, AA vs. C), but the rate of leucine oxidation increased 43% in the AA group (2.63 ± 0.16 vs. 1.84 ± 0.24 μmol·min−1·kg−1, P < 0.05). Fetal oxygen utilization tended to be increased in the AA group (327 ± 23 vs. 250 ± 29 μmol·min−1·kg−1, P = 0.06). Rates of leucine incorporation into fetal protein (5.19 ± 0.97 vs. 5.47 ± 0.89 μmol·min−1·kg−1, AA vs. C), release from protein breakdown (4.20 ± 0.95 vs. 4.62 ± 0.74 μmol·min−1·kg−1), and protein accretion (1.00 ± 0.30 vs. 0.85 ± 0.25 μmol·min−1·kg−1) did not change. Consistent with these data, there was no change in the fetal skeletal muscle ubiquitin ligases MaFBx1 or MuRF1 or in the protein synthesis regulators 4E-BP1, eEF2, eIF2α, and p70S6K. Decreased concentrations of certain essential amino acids, increased amino acid oxidation, fetal acidosis, and fetal hypoxia are possible mechanisms to explain fetal toxicity during maternal amino acid supplementation.

scholarly journals Alleviation of Dyslipidemia via a Traditional Balanced Korean Diet Represented by a Low Glycemic and Low Cholesterol Diet in Obese Women in a Randomized Controlled Trial

Nutrients ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 235
Author(s):  
Min Jung Kim ◽  
Sunmin Park ◽  
Hye Jeong Yang ◽  
Phil-Kyung Shin ◽  
Haeng Jeon Hur ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Amino Acids ◽  
Lipid Metabolism ◽  
Control Diet ◽  
Resistance Index ◽  
Diet Group ◽  
Plasma Metabolites ◽  
Obese Women ◽  
Glucose And Lipid Metabolism ◽  
Target Values

A traditional balanced Korean diet (K-diet) may improve energy, glucose, and lipid metabolism. To evaluate this, we conducted a randomized crossover clinical trial, involving participants aged 30–40 years, who were randomly assigned to two groups—a K-diet or westernized Korean control diet daily, with an estimated energy requirement (EER) of 1900 kcal. After a 4-week washout period, they switched the diet and followed it for 4 weeks. The carbohydrate, protein, and fat ratios based on energy intake were close to the target values for the K-diet (65:15:20) and control diet (60:15:25). The glycemic index of the control diet and the K-diet was 50.3 ± 3.6 and 68.1 ± 2.9, respectively, and daily cholesterol contents in the control diet and K-diet were 280 and 150 mg, respectively. Anthropometric and biochemical parameters involved in energy, glucose, and lipid metabolism were measured while plasma metabolites were determined using UPLC-QTOF-MS before and after the 4-week intervention. After the four-week intervention, both diets improved anthropometric and biochemical variables, but the K-diet significantly reduced them compared to the control diet. Serum total cholesterol, non-high-density lipoprotein cholesterol, and triglyceride concentrations were significantly lower in the K-diet group than in the control diet group. The waist circumference (p = 0.108) and insulin resistance index (QUICKI, p = 0.089) tended to be lower in the K-diet group than in the control diet group. Plasma metabolites indicated that participants in the K-diet group tended to reduce insulin resistance compared to those in the control diet group. Amino acids, especially branched-chain amino acids, tyrosine, tryptophan, and glutamate, and L-homocysteine concentrations were considerably lower in the K-diet group than in the control diet group (p < 0.05). Plasma glutathione concentrations, an index of antioxidant status, and 3-hydroxybutyric acid concentrations, were higher in the K-diet group than in the control diet group. In conclusion, a K-diet with adequate calories to meet EER alleviated dyslipidemia by decreasing insulin resistance-related amino acids and increasing ketones in the circulation of obese women.

Plasma amino acids, prolactin, insulin and glucose concentrations in lactating sows following venous infusion of isoleucine, leucine, lysine, threonine or valine

2014 ◽  
Vol 94 (2) ◽  
pp. 323-330 ◽  
Author(s):  
K. A. G. de Ridder ◽  
C. Farmer ◽  
C. F. M. de Lange ◽  
A. K. Shoveller ◽  
P. H. Luimes
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Plasma Concentrations ◽  
Daily Intake ◽  
Repeated Measurements ◽  
Saline Control ◽  
Plasma Amino Acids ◽  
Isoleucine Leucine ◽  
Lactating Sows ◽  
Venous Infusion

de Ridder, K. A. G., Farmer, C., de Lange, C. F. M., Shoveller, A. K. and Luimes, P. H. 2014. Plasma amino acids, prolactin, insulin and glucose concentrations in lactating sows following venous infusion of isoleucine, leucine, lysine, threonine or valine. Can. J. Anim. Sci. 94: 323–330. The amino acid supply to lactating sows affects their milk yield, and the current study aims at establishing whether this is mediated via increases in concentrations of insulin and prolactin, two hormones that are important for lactation. Six multiparous Yorkshire sows (252.8±18.5 kg body weight) were fitted with an ear vein catheter and were used in a 6×6 Latin square design experiment from days 7 to 10 of lactation (am and pm infusions on 3 consecutive days). Sows were fed a commercial corn and soybean meal diet (17.1% crude protein) three times daily (1.5 kg at 0600 and 1200, 3.0 kg at 1830). Starting 2 h after the 0600 or 1200 feeding, sows received an infusion of saline (control), isoleucine, leucine, lysine, threonine or valine at a rate of 33 mL min−1during 30 min. The infused dose was equivalent to 18±0.9% of true ileal digestible intake for each of the amino acids. Serial blood samples were taken, starting 30 min prior to infusion and until 240 min after infusion. Plasma concentrations of amino acids, prolactin, insulin, and glucose were determined. Data were analyzed as repeated measurements and also using areas under the curve. Intravenous infusion with each of the five amino acids studied increased the corresponding free plasma amino acid concentrations (P<0.05). However, concentrations of prolactin, insulin, and glucose were not affected by infusion of any of the amino acids studied (P>0.10). Therefore, supplying intravenously 18% of the daily intake of isoleucine, leucine, lysine, threonine or valine brings about expected increases in amino acids, but does not alter the short-term response in prolactin, insulin or glucose concentrations of lactating sows.

scholarly journals Diet reduction to requirements in obese/overfed ewes from early gestation prevents glucose/insulin dysregulation and returns fetal adiposity and organ development to control levels

2013 ◽  
Vol 305 (7) ◽  
pp. E868-E878 ◽  
Author(s):  
Nuermaimaiti Tuersunjiang ◽  
John F. Odhiambo ◽  
Nathan M. Long ◽  
Desiree R. Shasa ◽  
Peter W. Nathanielsz ◽  
...  
Keyword(s):  
Maternal Obesity ◽  
Maternal Diet ◽  
Control Diet ◽  
Plasma Concentrations ◽  
National Research Council ◽  
Late Gestation ◽  
Early Gestation ◽  
Fetal Organ ◽  
Insulin Dysregulation ◽  
Fetal Size

Obesity at conception and excess gestational weight gain pose significant risks for adverse health consequences in human offspring. This study evaluated the effects of reducing dietary intake of obese/overfed ewes beginning in early gestation on fetal development. Sixty days prior to conception, ewes were assigned to a control diet [CON: 100% of National Research Council (NRC) recommendations], a diet inducing maternal obesity (MO: 150% of NRC recommendations), or a maternal obesity intervention diet (MOI: 150% of NRC recommendations to day 28 of gestation, then 100% NRC) until necropsy at midgestation ( day 75) or late ( day 135) gestation. Fetal size and weight, as well as fetal organ weights, were greater ( P < 0.05) at midgestation in MO ewes than those of CON and MOI ewes. By late gestation, whereas fetal size and weight did not differ among dietary groups, cardiac ventricular weights and wall thicknesses as well as liver and perirenal fat weights remained elevated in fetuses from MO ewes compared with those from CON and MOI ewes. MO ewes and fetuses exhibited elevated ( P < 0.05) plasma concentrations of triglycerides, cholesterol, insulin, glucose, and cortisol at midgestation compared with CON and MOI ewes and fetuses. In late gestation, whereas plasma triglycerides and cholesterol, insulin, and cortisol remained elevated in MO vs. CON and MOI ewes and fetuses, glucose concentrations were elevated in both MO and MOI fetuses compared with CON fetuses, which was associated with elevated placental GLUT3 expression in both groups. These data are consistent with the concept that reducing maternal diet of obese/overfed ewes to requirements from early gestation can prevent subsequent alterations in fetal growth, adiposity, and glucose/insulin dynamics.

scholarly journals Portal net appearance of amino acids in growing pigs fed a barley-based diet with inclusion of three different forage meals

2000 ◽  
Vol 84 (4) ◽  
pp. 483-494 ◽  
Author(s):  
M. Reverter ◽  
T. Lundh ◽  
H. L. Gonda ◽  
J. E. Lindberg
Keyword(s):  
Amino Acids ◽  
Portal Vein ◽  
Mesenteric Artery ◽  
Control Diet ◽  
Growing Pigs ◽  
Fresh Weight ◽  
Young Pigs ◽  
Hepatic Portal Vein ◽  
Arterial Plasma ◽  
Hepatic Portal

The net absorption of amino acids (AA) in young pigs fed a barley-based control diet (C) and diets where barley was replaced by 200 g/kg fresh weight of dried lucerne (Medicago sativa; L20), white clover (Trifolium repens; W20) or perennial ryegrass (Lolium perenne; PR20) meal was studied. Castrated male pigs were fitted with permanent catheters in the hepatic portal vein and mesenteric artery, and the hepatic portal net absorption of AA was estimated from the porto–arterial plasma concentration differences and the hepatic portal-vein blood flow. In general, the essential AA (EAA) concentrations in the hepatic portal vein reached peak levels 90 min after feeding and thereafter exhibited a transient decline. Maximum porto–arterial differences were reached between 1 and 3 h postprandially for most of the AA. The cumulative net absorption of non-essential AA (NEAA) and EAA did not differ significantly between the barley-based diet and diets W20 and PR20. Due to a lower intake of AA on diet L20, the cumulative net absorption of NEAA and EAA was significantly (P<0·05) lower than diet C. With the exceptions of the EAA arginine, cystine and valine, and the NEAA glutamic acid + glutamine and glycine, there were no significant differences in the absorption coefficients for the EAA and NEAA between the diets. In addition, the pattern of the total EAA in the mixture absorbed postprandially did not differ significantly between the diets. The present study gives support to the contention that the replacement of barley AA with forage meal AA in a barley-based diet for growing pigs should be expected to result in minor differences in the net portal flux of AA.

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