scholarly journals Potency Assessment of CBD Oils by Their Effects on Cell Signaling Pathways

Nutrients ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 357 ◽  
Author(s):  
Yasuyo Urasaki ◽  
Cody Beaumont ◽  
Michelle Workman ◽  
Jeffery N. Talbot ◽  
David K. Hill ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Posttranslational Modification ◽  
Biological Activities ◽  
Neuronal Cell ◽  
Effective Concentration ◽  
Composition Analysis ◽  
Measurable Effect ◽  
Mtor Signaling Pathway ◽  
Chemical Composition Analysis ◽  
Cell Signaling Pathways

This study used nanofluidic protein posttranslational modification (PTM) profiling to measure the effects of six cannabidiol (CBD) oils and isolated CBD on the signaling pathways of a cultured SH-SY5Y neuronal cell line. Chemical composition analysis revealed that all CBD oils met the label claims and legal regulatory limit regarding the CBD and tetrahydrocannabinol (THC) contents, respectively. Isolated CBD was cytotoxic, with an effective concentration (EC50) of 40 µM. In contrast, the CBD oils had no effect on cell viability at CBD concentrations exceeding 1.2 mM. Interestingly, only an unadulterated CBD oil had strong and statistically significant suppressive effects on the pI3K/Akt/mTOR signaling pathway with an EC50 value of 143 µM and a slow-acting timescale requiring hours. Systematic profiling of twenty-six proteins, which served as biomarkers for nine signaling pathways, revealed that the unadulterated CBD oil downregulated seven signaling pathways but had no measurable effect on the other two signaling pathways. The remaining CBD oils, which were adulterated, and isolated CBD had weak, variable, or undetectable effects on neuronal signaling pathways. Our data clearly showed that adulteration diminished the biological activities of CBD oils. In addition, nanofluidic protein PTM profiling provided a robust means for potency assessment of CBD oils.

scholarly journals Fast-Acting and Receptor-Mediated Regulation of Neuronal Signaling Pathways by Copaiba Essential Oil

2020 ◽  
Vol 21 (7) ◽  
pp. 2259 ◽  
Author(s):  
Yasuyo Urasaki ◽  
Cody Beaumont ◽  
Michelle Workman ◽  
Jeffery N. Talbot ◽  
David K. Hill ◽  
...  
Keyword(s):  
Essential Oil ◽  
Signaling Pathways ◽  
Signaling Pathway ◽  
Cannabinoid Receptor ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Neuronal Cell ◽  
Mtor Signaling ◽  
Mtor Signaling Pathway ◽  
Fast Acting

This study examined the biological activities of copaiba essential oil via measurement of its effects on signaling pathways in the SH-SY5Y neuronal cell line. Nanofluidic proteomic technologies were deployed to measure the phosphorylation of biomarker proteins within the signaling cascades. Interestingly, copaiba essential oil upregulated the pI3K/Akt/mTOR, MAPK, and JAK/STAT signaling pathways in neuronal cells. The effects of copaiba essential oil peaked at 30 min post-treatment, with a half-maximal effective concentration (EC50) of approximately 80 ng/mL. Treatment with cannabinoid receptor 2 (CB2) agonist AM1241 or the inverse agonist BML190 abrogated the regulatory effects of copaiba essential oil on the pI3K/Akt/mTOR signaling pathway. Surprisingly, copaiba essential oil also activated the apoptosis signaling pathway and reduced the viability of SH-SY5Y cells with an EC50 of approximately 400 ng/mL. Furthermore, β-caryophyllene, a principal constituent of copaiba essential oil, downregulated the pI3K/Akt/mTOR signaling pathway. Taken together, the findings indicated that copaiba essential oil upregulated signaling pathways associated with cell metabolism, growth, immunity, and apoptosis. The biological activities of copaiba essential oil were determined to be fast acting, CB2 mediated, and dependent on multiple chemical constituents of the oil. Nanofluidic proteomics provided a powerful means to assess the biological activities of copaiba essential oil.

scholarly journals Curcumin and Cancer

Nutrients ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 2376 ◽  
Author(s):  
Giordano ◽  
Tommonaro
Keyword(s):  
Transcription Factors ◽  
Growth Factors ◽  
Cancer Therapy ◽  
Cell Signaling ◽  
Signaling Pathways ◽  
Inflammatory Cytokines ◽  
Biological Activities ◽  
Curcuma Longa ◽  
Cancer Development ◽  
Cell Signaling Pathways

Curcumin, a polyphenol extracted from Curcuma longa in 1815, has gained attention from scientists worldwide for its biological activities (e.g., antioxidant, anti-inflammatory, antimicrobial, antiviral), among which its anticancer potential has been the most described and still remains under investigation. The present review focuses on the cell signaling pathways involved in cancer development and proliferation, and which are targeted by curcumin. Curcumin has been reported to modulate growth factors, enzymes, transcription factors, kinase, inflammatory cytokines, and proapoptotic (by upregulation) and antiapoptotic (by downregulation) proteins. This polyphenol compound, alone or combined with other agents, could represent an effective drug for cancer therapy.

scholarly journals Inhibition Effect of Chloroquine and Integrin-Linked Kinase Knockdown on Translation in Melanoma Cells

2021 ◽  
Vol 22 (7) ◽  
pp. 3682
Author(s):  
Dorota Gil ◽  
Piotr Laidler ◽  
Marta Zarzycka ◽  
Joanna Dulińska-Litewka
Keyword(s):  
Signaling Pathways ◽  
Melanoma Cell ◽  
Melanoma Cells ◽  
Antitumor Effects ◽  
Regulatory Pathways ◽  
Scratch Wound ◽  
Integrin Linked Kinase ◽  
And Migration ◽  
Cell Signaling Pathways

The twofold role of autophagy in cancer is often the therapeutic target. Numerous regulatory pathways are shared between autophagy and other molecular processes needed in tumorigenesis, such as translation or survival signaling. Thus, we have assumed that ILK knockdown should promote autophagy, and used together with chloroquine, an autophagy inhibitor, it could generate a better anticancer effect by dysregulation of common signaling pathways. Expression at the protein level was analyzed using Western Blot; siRNA transfection was done for ILK. Analysis of cell signaling pathways was monitored with phospho-specific antibodies. Melanoma cell proliferation was assessed with the crystal violet test, and migration was evaluated by scratch wound healing assays. Autophagy was monitored by the accumulation of its marker, LC3-II. Our data show that ILK knockdown by siRNA suppresses melanoma cell growth by inducing autophagy through AMPK activation, and simultaneously initiates apoptosis. We demonstrated that combinatorial treatment of melanoma cells with CQ and siILK has a stronger antitumor effect than monotherapy with either of these. It generates the synergistic antitumor effects by the decrease of translation of both global and oncogenic proteins synthesis. In our work, we point to the crosstalk between translation and autophagy regulation.

scholarly journals Correction: Yu, D.Q., et al. Microscopic Characteristic and Chemical Composition Analysis of Three Medicinal Plants and Surface Frosts. Molecules 2019, 24, 4548

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 379
Author(s):  
Da Qing Yu ◽  
Xiao Jing Han ◽  
Ting Yu Shan ◽  
Rui Xu ◽  
Jin Hu ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Medicinal Plants ◽  
Composition Analysis ◽  
Microscopic Characteristic ◽  
Chemical Composition Analysis

The authors would like to correct an error in the title paper [...]

Cell signaling pathways as molecular targets to eliminate AML stem cells

2021 ◽  
Vol 160 ◽  
pp. 103277
Author(s):  
Ana Carolina B. da C. Rodrigues ◽  
Rafaela G.A. Costa ◽  
Suellen L.R. Silva ◽  
Ingrid R.S.B. Dias ◽  
Rosane B. Dias ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Signaling ◽  
Signaling Pathways ◽  
Molecular Targets ◽  
Cell Signaling Pathways

scholarly journals Dietary polyphenols suppress chronic inflammation by modulation of multiple inflammation-associated cell signaling pathways

2021 ◽  
pp. 108634
Author(s):  
Ibrahim Jantan ◽  
Md. Areeful Haque ◽  
Laiba Arshad ◽  
Hemavathy Harikrishnan ◽  
Abdi Wira Septama ◽  
...  
Keyword(s):  
Cell Signaling ◽  
Signaling Pathways ◽  
Chronic Inflammation ◽  
Dietary Polyphenols ◽  
Cell Signaling Pathways
Sign in / Sign up

Export Citation Format

Share Document