scholarly journals Wheat Extract Oil (WEO) Attenuates UVB-Induced Photoaging via Collagen Synthesis in Human Keratinocytes and Hairless Mice

Nutrients ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 300 ◽  
Author(s):  
Dong Ju Son ◽  
Jae Chul Jung ◽  
Yong Min Choi ◽  
Hyeon Yeol Ryu ◽  
Somin Lee ◽  
...  
Keyword(s):  
Oral Administration ◽  
Barrier Function ◽  
Collagen Synthesis ◽  
Skin Barrier ◽  
Human Keratinocytes ◽  
Water Evaporation ◽  
Skin Barrier Function ◽  
Type I ◽  
Skin Damage ◽  
Hairless Mice

The efficacy of wheat extract oil (WEO), standardized to glucosylceramides, for protecting against ultraviolet B (UVB)-induced damage of skin barrier function was assessed using the SHK-1 hairless mouse model and two human skin cell lines, namely, CCD-986sk and HeCaT. The ability of repeated oral administration of 30, 60, and 120 mg of WEO/kg/day for 12 weeks to prevent skin damage of SKH-1 hairless mice induced by UVB irradiation was evaluated. The results demonstrated that UVB-induced water evaporation (transepidermal water loss, TEWL) was significantly decreased by WEO. Similarly, UVB-induced losses in moisture and skin elasticity were improved by WEO supplementation. WEO attenuated the tissue procollagen type I, hyaluronic acid (HA), and ceramide reductions induced by UVB treatment as well. Collagen concentrations in skin tissue were increased in the WEO-treated mice, while UVB-induced epidermal thickening was reduced. In vitro studies using HeCaT human keratinocytes confirmed increased HA and collagen synthesis in response to WEO treatment. This may occur via WEO suppression of matrix metalloproteinase-1 (MMP-1), since its induction by UVB treatment was diminished in treated CCD-986sk cells. Oral administration of WEO improves skin barrier function in UVB-irradiated mice by attenuating damage typically observed in photoaging. This research further clarifies the clinical benefits previously observed by dietary WEO consumption.

scholarly journals Wheat Extract Oil (WEO) Attenuates UVB-Induced Photoaging via Collagen Synthesis in Human Keratinocytes and Hairless Mice

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 387-387
Author(s):  
Barbara Davis ◽  
Dong Ju Son ◽  
Jae Chul Jung ◽  
Yong Min Choi ◽  
Hyeon Yeol Ryu ◽  
...  
Keyword(s):  
Oral Administration ◽  
Barrier Function ◽  
Collagen Synthesis ◽  
Skin Barrier ◽  
Human Keratinocytes ◽  
Water Evaporation ◽  
Skin Barrier Function ◽  
Type I ◽  
Skin Damage ◽  
Hairless Mice

Abstract Objectives The efficacy of wheat extract oil (WEO), standardized to glucosylceramides, for protecting against ultraviolet B (UVB)-induced damage of skin barrier function was assessed using the SHK-1 hairless mouse model and two human skin cell lines, namely, CCD-986sk and HeCaT. Methods The ability for repeated oral administration of 30, 60, and 120 mg of WEO/kg/day for 12 weeks to prevent skin damage of SKH-1 hairless mice induced by UVB irradiation was evaluated. To complement this work, and better understand the mechanism(s) through which this dietary ingredient works, changes in procollagen, hyaluronic acid (HA) and matrix metalloproteinase-1 (MMP-1) levels were assessed in response to UVB treatment in the presence and absence of WEO. Results The results demonstrated that UVB-induced water evaporation (transepidermal water loss, TEWL) was significantly decreased by WEO. Similarly, UVB-induced losses in moisture and skin elasticity were improved by WEO supplementation. WEO attenuated the tissue procollagen type I, HA, and ceramide reductions induced by UVB treatment as well. Collagen concentrations in skin tissue were increased in the WEO-treated mice, while UVB-induced epidermal thickening was reduced. In vitro studies using HeCaT human keratinocytes confirmed increased HA and collagen synthesis in response to WEO treatment. This may occur via WEO suppression of MMP-1, since its induction by UVB treatment was diminished in treated CCD-986sk cells. Conclusions Oral administration of WEO improves skin barrier function in UVB-irradiated mice by attenuating damage typically observed in photoaging. This research further clarifies the clinical benefits previously observed by dietary WEO consumption. Funding Sources Funding for this research was provided by the Life Science Research Institute, Novarex Co., Ltd.

Dietary glucosylceramide improves skin barrier function in hairless mice

2006 ◽  
Vol 44 (2) ◽  
pp. 101-107 ◽  
Author(s):  
Kiyomi Tsuji ◽  
Susumu Mitsutake ◽  
Junko Ishikawa ◽  
Yutaka Takagi ◽  
Masashi Akiyama ◽  
...  
Keyword(s):  
Barrier Function ◽  
Skin Barrier ◽  
Skin Barrier Function ◽  
Hairless Mice

Effect of Camellia japonica oil on human type I procollagen production and skin barrier function

2007 ◽  
Vol 112 (1) ◽  
pp. 127-131 ◽  
Author(s):  
Eunsun Jung ◽  
Jongsung Lee ◽  
Jihoon Baek ◽  
Kwangsun Jung ◽  
Jiyoung Lee ◽  
...  
Keyword(s):  
Barrier Function ◽  
Skin Barrier ◽  
Skin Barrier Function ◽  
Type I ◽  
Camellia Japonica ◽  
Human Type ◽  
Type I Procollagen

Clove attenuates UVB-induced photodamage and repairs skin barrier function in hairless mice

2018 ◽  
Vol 9 (9) ◽  
pp. 4936-4947 ◽  
Author(s):  
Eunson Hwang ◽  
Pei Lin ◽  
Hien T. T. Ngo ◽  
Tae-Hoo Yi
Keyword(s):  
Barrier Function ◽  
Food Industry ◽  
Skin Barrier ◽  
Skin Barrier Function ◽  
Syzygium Aromaticum ◽  
Hairless Mice

Syzygium aromaticum L., commonly named clove, is widely used in the food industry due to its antioxidant and antibacterial capabilities.

scholarly journals Physiological Effects of Manganese Gluconate and Calcium Combination from Saint-Gervais Mont Blanc Spring Water for Human Keratinocytes Differentiation

2021 ◽  
Vol 7 (2) ◽  
pp. 1-6
Author(s):  
Franck Juchaux ◽  
Keyword(s):  
Barrier Function ◽  
Skin Barrier ◽  
Human Keratinocytes ◽  
Spring Water ◽  
Keratinocyte Differentiation ◽  
Skin Barrier Function ◽  
Transglutaminase 1

Alterations of skin barrier function affect quality of life and there is a need to develop dermatological/cosmetic treatments to reinforce or restore it. Inspiring of Hailey-Hailey disease, in which barrier alteration is due to a mutation of a Calcium-transporting protein (ATP2C1), we focused on the role of minerals and more especially those contained in Saint-Gervais Mont Blanc (SGMB) spring water to reinforce barrier function. Objectives: Demonstrate the interest to enrich SGMB spring water with manganese to improve both keratinocytes differentiation and barrier function. Methods: Effects of treatments on the expression of ATP2C1 and on the expression of key markers in keratinocyte differentiation and barrier function were studied by gene expression analysis on keratinocytes monolayers and also by measuring the protein expression of transglutaminase 1 using in situ immunofluorescence and image analysis in keratinocytes monolayers. Results: SGMB spring water stimulates transcriptomic expression of key markers involved in keratinocytes differentiation and barrier function while manganese gluconate has no effect. Combination of both dramatically enhances keratinocytes differentiation, in a synergistic way, at both transcriptomic and protein level. None of treatments modulated ATP2C1 expression. Conclusion: These results highlight the interest to enrich SGMB spring water with manganese to boost keratinocytes differentiation and barrier function.

scholarly journals Antagonistic Effects of IL-4 on IL-17A-Mediated Enhancement of Epidermal Tight Junction Function

2019 ◽  
Vol 20 (17) ◽  
pp. 4070 ◽  
Author(s):  
Matthew G. Brewer ◽  
Takeshi Yoshida ◽  
Fiona I. Kuo ◽  
Sade Fridy ◽  
Lisa A. Beck ◽  
...  
Keyword(s):  
Tight Junction ◽  
Barrier Function ◽  
Skin Barrier ◽  
Protein Composition ◽  
Human Keratinocytes ◽  
Skin Barrier Function ◽  
Primary Human Keratinocytes ◽  
Tight Junction Formation ◽  
And Function ◽  
The Impact

Atopic dermatitis (AD) is the most common chronic and relapsing inflammatory skin disease. AD is typically characterized by skewed T helper (Th) 2 inflammation, yet other inflammatory profiles (Th1, Th17, Th22) have been observed in human patients. How cytokines from these different Th subsets impact barrier function in this disease is not well understood. As such, we investigated the impact of the canonical Th17 cytokine, IL-17A, on barrier function and protein composition in primary human keratinocytes and human skin explants. These studies demonstrated that IL-17A enhanced tight junction formation and function in both systems, with a dependence on STAT3 signaling. Importantly, the Th2 cytokine, IL-4 inhibited the barrier-enhancing effect of IL-17A treatment. These observations propose that IL-17A helps to restore skin barrier function, but this action is antagonized by Th2 cytokines. This suggests that restoration of IL-17/IL-4 ratio in the skin of AD patients may improve barrier function and in so doing improve disease severity.

scholarly journals Dietary Ceramide Prepared from Soy Sauce Lees Improves Skin Barrier Function in Hairless Mice

2021 ◽  
Author(s):  
Kazushi Ohta ◽  
Shinobu Hiraki ◽  
Masakatsu Miyanabe ◽  
Tatsuro Ueki ◽  
Yuki Manabe ◽  
...  
Keyword(s):  
Barrier Function ◽  
Skin Barrier ◽  
Soy Sauce ◽  
Skin Barrier Function ◽  
Hairless Mice

scholarly journals Dietary Milk Sphingomyelin Prevents Disruption of Skin Barrier Function in Hairless Mice after UV-B Irradiation

PLoS ONE ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. e0136377 ◽  
Author(s):  
Chisato Oba ◽  
Masashi Morifuji ◽  
Satomi Ichikawa ◽  
Kyoko Ito ◽  
Keiko Kawahata ◽  
...  
Keyword(s):  
Barrier Function ◽  
Skin Barrier ◽  
Skin Barrier Function ◽  
Hairless Mice
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