scholarly journals The Effect of Donor Human Milk Fortification on The Adhesion of Probiotics In Vitro

Nutrients ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 182 ◽  
Author(s):  
Anastasia Mantziari ◽  
Satu Tölkkö ◽  
Artur C. Ouwehand ◽  
Eliisa Löyttyniemi ◽  
Erika Isolauri ◽  
...  
Keyword(s):  
Human Milk ◽  
Liquid Scintillation ◽  
Adhesion Properties ◽  
Protein Supplements ◽  
Donor Human Milk ◽  
Probiotic Strains ◽  
Human Milk Fortifier ◽  
The Impact ◽  
And Storage

Preterm delivery complications are the primary cause of death among children under the age of five. Preventive strategies include the use of pasteurized donor human milk (DHM), its fortification with human milk fortifiers (protein supplements), and supplementation with probiotics. Our aim was to examine the impact of DHM and fortified DHM (FDHM) on the mucus adhesion properties of two widely used probiotics. The study covered two forms of human milk fortifier, liquid and powdered, with or without probiotics and storage at 4 °C for 24 h. To test the adhesion properties of the probiotic strains, DHM+probiotics and FDHM+probiotics were prepared and added to immobilized mucus isolated from the stool of healthy Finnish infants. The probiotic adhesion was then measured by liquid scintillation. Our results suggest that addition of liquid or powdered human milk fortifier in donor human milk had no impact on probiotic adhesion. In addition, given the increased adhesion of probiotics suspended in buffer, other matrices should be further studied. These factors need to be considered when designing future intervention strategies using probiotics in preterm infants.

scholarly journals Addition of Calcium and Phosphorus to Preterm Donor Human Milk and the Impact on Protein, Fat, and Calcium Digestibility In Vitro

2012 ◽  
Vol 4 (4) ◽  
pp. 199-206 ◽  
Author(s):  
April Danielle Fogleman ◽  
Ronald S. Cohen ◽  
Pauline Sakamoto ◽  
Jonathan C. Allen
Keyword(s):  
Human Milk ◽  
Donor Human Milk ◽  
Calcium And Phosphorus ◽  
The Impact

The impact of early gut microbiota modulation on the risk of child disease: alert to accuracy in probiotic studies

2015 ◽  
Vol 6 (2) ◽  
pp. 167-171 ◽  
Author(s):  
E. Isolauri ◽  
S. Salminen
Keyword(s):  
Gut Microbiota ◽  
Allergic Inflammation ◽  
Clinical Intervention ◽  
Probiotic Potential ◽  
Inflammatory State ◽  
Probiotic Strains ◽  
Risk Of Disease ◽  
Different Populations ◽  
The Impact ◽  
And Storage

The composition of the gut microbiota, and thus also the modification of the gut microbiota by specific probiotics or prebiotics early in life, may have an impact on the risk of disease in the child. Above the impact on gut microecology, probiotic effects have been attributed to restoration to normal of increased intestinal permeability, improvement of the intestine's immunological barrier functions, alleviation of the intestinal inflammatory response, and reduced generation of proinflammatory cytokines characteristic of local and systemic allergic inflammation. Recent demonstrations from experimental and clinical studies suggest that the gut microbiota is also involved in the control of body weight and energy metabolism, affecting the two main causes of obesity: energy acquisition and storage, and contributing to insulin resistance and the inflammatory state characterising obesity. Current research focuses both on characterising specific probiotic strains and on how the food matrix and the dietary content interacts with the most efficient probiotic strains. It is important to characterise each probiotic to species and strain level and to select strains with documented properties, the probiotic potential being strain-specific. As any proof of causality requires clinical intervention studies in humans in different populations, rigorous and detailed documentation will enhance reproducibility and circumvent confusion.

scholarly journals Influence of Pasteurization and Storage on Dynamic In Vitro Gastric Digestion of Milk Proteins: Quantitative Insights Based on Peptidomics

Foods ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 998 ◽  
Author(s):  
Xing Li ◽  
Yuxiang Gu ◽  
Shudong He ◽  
Olayemi Eyituoyo Dudu ◽  
Qiming Li ◽  
...  
Keyword(s):  
Cold Storage ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Milk Proteins ◽  
Hydrolysis Rate ◽  
Gastric Digestion ◽  
Pasteurized Milk ◽  
The Impact ◽  
And Storage

It is important to evaluate the nutritional quality of milk during the shelf-life, especially during home storage, from a consumer viewpoint. In this study, we investigated the impact of pasteurization (85 °C/15 s) and subsequent storage (at 4 °C for 7 days) on the coagulation behavior of milk and protein digestibility in a dynamic in vitro gastric digestion test. A high level of hydration in curd formed in pasteurized milk upon 7-day cold storage compared to raw and pasteurized milk, indicating fast pepsin diffusion in the interior of curds, increasing the hydrolysis rate. The digesta collected at various time points throughout the gastric digestion were studied using o-phthaldialdehyde (OPA), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), liquid chromatography tandem mass spectrometry (LC-MS/MS), and amino acid analysis. These results showed that milk proteins were hydrolyzed quickly upon a long period of cold storage. Additionally, qualitative and quantitative results obtained using LC-MS/MS exhibited significant differences between samples, especially in pasteurized milk upon cold storage. Processing and storage played a decisive role in bioactive peptide generation. Such knowledge could provide insights into and directions for the storage of pasteurized milk for further clinical studies on protein bioavailability and the generation of bioactive peptides for desired health outcomes.

scholarly journals The Effect of Holder Pasteurization and Storage on Macronutrients in Donor Human Milk

Perinatology ◽  
2020 ◽  
Vol 31 (1) ◽  
pp. 26
Author(s):  
Jinjoo Kim ◽  
Na Mi Lee ◽  
Dae Yong Yi ◽  
Sung-Hoon Chung ◽  
Yong-Sung Choi
Keyword(s):  
Human Milk ◽  
Donor Human Milk ◽  
And Storage

scholarly journals The Impact of Lactoferrin on the Growth of Intestinal Inhabitant Bacteria

2019 ◽  
Vol 20 (19) ◽  
pp. 4707 ◽  
Author(s):  
Alan Vega-Bautista ◽  
Mireya de la Garza ◽  
Julio César Carrero ◽  
Rafael Campos-Rodríguez ◽  
Marycarmen Godínez-Victoria ◽  
...  
Keyword(s):  
Inflammatory Responses ◽  
Cell Monolayer ◽  
Multidrug Resistant ◽  
Nerve Fibers ◽  
Gut Homeostasis ◽  
Life Threatening ◽  
Probiotic Strains ◽  
The Impact

Lactoferrin (Lf) is an iron-binding milk glycoprotein that promotes the growth of selected probiotic strains. The effect of Lf on the growth and diversification of intestinal microbiota may have an impact on several issues, including (i) strengthening the permeability of the epithelial cell monolayer, (ii) favoring the microbial antagonism that discourages the colonization and proliferation of enteric pathogens, (iii) enhancing the growth and maturation of cell-monolayer components and gut nerve fibers, and (iv) providing signals to balance the anti- and pro-inflammatory responses resulting in gut homeostasis. Given the beneficial role of probiotics, this contribution aims to review the current properties of bovine and human Lf and their derivatives in in vitro probiotic growth and Lf interplay with microbiota described in the piglet model. By using Lf as a component in pharmacological products, we may enable novel strategies that promote probiotic growth while conferring antimicrobial activity against multidrug-resistant microorganisms that cause life-threatening diseases, especially in neonates.

scholarly journals Three Novel Candidate Probiotic Strains with Prophylactic Properties in a Murine Model of Cow's Milk Allergy

2016 ◽  
Vol 82 (6) ◽  
pp. 1722-1733 ◽  
Author(s):  
Elodie Neau ◽  
Johanne Delannoy ◽  
Candice Marion ◽  
Charles-Henry Cottart ◽  
Chantal Labellie ◽  
...  
Keyword(s):  
Murine Model ◽  
Food Allergies ◽  
T Helper ◽  
Murine Splenocytes ◽  
Content Type ◽  
Cellular Models ◽  
Probiotic Strains ◽  
The Impact

ABSTRACTFood allergies can have significant effects on morbidity and on quality of life. Therefore, the development of efficient approaches to reduce the risk of developing food allergies is of considerable interest. The aim of this study was to identify and select probiotic strains with preventive properties against allergies using a combination ofin vitroandin vivoapproaches. To that end, 31 strains of bifidobacteria and lactic acid bacteria were screened for their immunomodulatory properties in two cellular models, namely, human peripheral blood mononuclear cells (PBMCs) and T helper 2 (Th2)-skewed murine splenocytes. Six strains inducing a high interleukin-10 (IL-10)/IL-12p70 ratio and a low secretion of IL-4 on the two cellular models were selected, and their protective impact was testedin vivoin a murine model of food allergy to β-lactoglobulin. Three strains showed a protective impact on sensitization, with a decrease in allergen-specific IgE, and on allergy, with a decrease in mast cell degranulation. Analysis of the impact of these three strains on the T helper balance revealed different mechanisms of action. TheLactobacillus salivariusLA307 strain proved to block Th1 and Th2 responses, while theBifidobacterium longumsubsp.infantisLA308 strain induced a pro-Th1 profile and theLactobacillus rhamnosusLA305 strain induced pro-Th1 and regulatory responses. These results demonstrate that a combination ofin vitroandin vivoscreening is effective in probiotic strain selection and allowed identification of three novel probiotic strains that are active against sensitization in mice.

scholarly journals Inoculation of mother’s own milk could personalize pasteurized donor human milk used for feeding preterm infants

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
D. Mallardi ◽  
C. Tabasso ◽  
P. Piemontese ◽  
S. Morandi ◽  
T. Silvetti ◽  
...  
Keyword(s):  
Human Milk ◽  
Preterm Infants ◽  
Bacterial Growth ◽  
Microbiological Quality ◽  
In Vitro Study ◽  
Total Bacterial Count ◽  
Milk Samples ◽  
Donor Human Milk ◽  
Mother’S Own Milk

Abstract Background Human milk is a vehicle for bioactive compounds and beneficial bacteria which promote the establishment of a healthy gut microbiome of newborns, especially of preterm infants. Pasteurized donor human milk (PDHM) is the second-best option when preterm mother’s own milk is unavailable. Since pasteurization affect the microbiological quality of donor milk, PDHM was inoculated with different preterm milk samples and then incubated, in order to evaluate the effect in terms of bacterial growth, human milk microbiome and proteolytic phenomena. Methods In an in-vitro study PDHM was inoculated at 10% v/v using ten preterm milk samples. Microbiological, metataxonomic and peptidomic analyses, on preterm milk samples at the baseline (T0), on PDHM and on inoculated milk (IM) samples at T0, after 2 h (T1) and 4 h (T2) of incubation at 37 °C, were conducted. Results IM samples at T2 showed a Total Bacterial Count not significantly different (p > 0.01) compared to preterm milk samples. At T2 lactic acid bacteria level was restored in all IM. After inoculation, metataxonomic analysis in IM samples showed that Proteobacteria remained the predominant phylum while Firmicutes moved from 3% at T1 to 9.4% at T2. Peptidomic profile of IM resembled that of PDHM, incubated for the same time, in terms of number and type of peptides. Conclusion The study demonstrated that inoculation of PDHM with mother’s own milk could restore bacterial growth and personalize human milk microbiome in PDHM. This effect could be beneficial because of the presence of maternal probiotic bacteria which make PDHM more similar to mother’s own milk.

scholarly journals THE IMPACT OF FRUCTOSE THE GROWTH AND STORAGE OF GRAPEVINE COLLECTION IN THE IN VITRO

2020 ◽  
Vol 6 (66) ◽  
pp. 184-197
Author(s):  
Natalia Petrovna Dorochenko ◽  
◽  
Valentina Georgievna Puzyrnova ◽  
Keyword(s):  
The Impact ◽  
And Storage

scholarly journals The Impact of Homogenization on Donor Human Milk and Human Milk-Based Fortifiers and Implications for Preterm Infant Health

2021 ◽  
Author(s):  
Sarah M Reyes ◽  
Biranchi Patra ◽  
Melinda J Elliott
Keyword(s):  
Human Milk ◽  
Preterm Infant ◽  
Milk Fat ◽  
Infant Health ◽  
Cow Milk ◽  
Published Data ◽  
Donor Human Milk ◽  
Milk Fat Globule ◽  
Health Complications ◽  
The Impact

Abstract An exclusive human milk diet (EHMD) has been shown to reduce health complications of prematurity in infants born weighing ≤1250 grams compared to cow milk-based diets. Accordingly, the number of available human milk (HM)-based nutritional products continues to increase. Newly available products, and those reportedly soon to enter the market include homogenized donor human milk (DHM) and homogenized HM–based fortifiers. Existing literature demonstrating the benefits of an EHMD, however, is limited to non-homogenized HM-based products. Herein, we summarize existing evidence on the impact of homogenization on HM, with a particular focus on changes to the macromolecular structure of the milk fat globule and its subsequent impact on digestion kinetics. We use these published data to create a conceptual framework for the potential implications of homogenized HM-based nutritional products on preterm infant health. Importantly, we underscore that the safety and efficacy of homogenized HM-based products warrant investigation.

Receiving Screened Donor Human Milk for Their Infant Supports Parental Wellbeing: a Mixed-methods Study

2021 ◽  
Author(s):  
Amy Brown ◽  
Natalie Shenker
Keyword(s):  
Mental Health ◽  
Human Milk ◽  
Infant Health ◽  
Positive Impact ◽  
Online Questionnaire ◽  
Donor Human Milk ◽  
Increased Risk ◽  
Health And Development ◽  
Almost All ◽  
The Impact

Abstract Background: Donor human milk (DHM) protects the health and development of premature infants but there has been little examination of the broader impact of an infant receiving it upon parental mental health. Breastfeeding and mental health are closely tied with women who experience breastfeeding difficulties or are unable to meet their own breastfeeding goals often experiencing feelings of guilt, sadness and anger, alongside an increased risk of postnatal depression. The aim of the current study was to explore how experience of receiving DHM for their baby affected the wellbeing of parents.Methods: UK parents of infants aged 0 – 12 months who had received screened DHM from a milk bank (typically on the neonatal unit or in some cases in the community) completed an online questionnaire exploring their experiences. The questionnaire included Likert scale items examining perceived impact upon infant health, own wellbeing and family functioning alongside open-ended questions exploring perceptions of how receiving DHM affected wellbeing.Results: Almost all of the 107 participants (women=102) agreed that receiving DHM had a positive impact upon infant health and development, their own mental and physical health, and their family’s wellbeing. Parents felt relieved that their infant was receiving DHM for health reasons but also due to the experience of being listened to, supported and having their infant feeding decisions facilitated. Receiving DHM helped mothers to process some of their emotions at not being able to breastfeed, in part because knowing their baby was being fed gave them the space to focus on recovery and bonding with their baby. Some parents did experience challenges, feeling guilty at receiving DHM, insecure that another woman was able to feed their baby when they could not, with some negative reactions from family. Although the impact of receiving DHM upon breastfeeding was not measured, some women who were working to build their own milk supply noted that it helped motivate them to continue.Conclusions: DHM may play an important role not only in protecting infant health and development but in supporting the mental health and wellbeing of mothers for whom their infant receiving human milk is important.

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