scholarly journals Dietary Micronutrient Supplementation for 12 Days in Obese Male Mice Restores Sperm Oxidative Stress

Nutrients ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 2196 ◽  
Author(s):  
Nicole O. McPherson ◽  
Helana Shehadeh ◽  
Tod Fullston ◽  
Deirdre L. Zander-Fox ◽  
Michelle Lane
Keyword(s):  
Oxidative Stress ◽  
Sperm Quality ◽  
Control Diet ◽  
Early Embryo ◽  
Micronutrient Supplementation ◽  
Micronutrient Deficiencies ◽  
Short Term ◽  
Fetal Outcomes ◽  
Partial Restoration ◽  
Cell Cleavage

Male obesity, which often co-presents with micronutrient deficiencies, is associated with sub-fertility. Here we investigate whether short-term dietary supplementation of micronutrients (zinc, selenium, lycopene, vitamins E and C, folic acid, and green tea extract) to obese mice for 12 days (designed to span the epididymal transit) could improve sperm quality and fetal outcomes. Five-week-old C57BL6 males were fed a control diet (CD, n = 24) or high fat diet (HFD, n = 24) for 10 weeks before allocation to the 12-day intervention of maintaining their original diets (CD, n = 12, HFD n = 12) or with micronutrient supplementation (CD + S, n = 12, HFD + S, n = 12). Measures of sperm quality (motility, morphology, capacitation, binding), sperm oxidative stress (DCFDA, MSR, and 8OHdG), early embryo development (2-cell cleavage, 8OHdG), and fetal outcomes were assessed. HFD + S males had reduced sperm intracellular reactive oxygen species (ROS) concentrations and 8OHdG lesions, which resulted in reduced 8OHdG lesions in the male pronucleus, increased 2-cell cleavage rates, and partial restoration of fetal weight similar to controls. Sub-fertility associated with male obesity may be restored with very short-term micronutrient supplementation that targets the timing of the transit of sperm through the epididymis, which is the developmental window where sperm are the most susceptible to oxidative damage.

scholarly journals Micronutrients and the pathogenesis of human immunodeficiency virus infection

1999 ◽  
Vol 81 (3) ◽  
pp. 181-189 ◽  
Author(s):  
R. D. Semba ◽  
A. M. Tang
Keyword(s):  
Oxidative Stress ◽  
Human Immunodeficiency Virus ◽  
Virus Infection ◽  
Hiv Infection ◽  
Serum Levels ◽  
Micronutrient Supplementation ◽  
Micronutrient Deficiencies ◽  
Immunodeficiency Virus ◽  
Altered Metabolism ◽  
Low Levels

Micronutrient deficiencies may be common during human immunodeficiency virus (HIV) infection. Insufficient dietary intake, malabsorption, diarrhoea, and impaired storage and altered metabolism of micronutrients can contribute to the development of micronutrient deficiencies. Low plasma or serum levels of vitamins A, E, B6, B12 and C, carotenoids, Se, and Zn are common in many HIV-infected populations. Micronutrient deficiencies may contribute to the pathogenesis of HIV infection through increased oxidative stress and compromised immunity. Low levels or intakes of micronutrients such as vitamins A, E, B6 and B12, Zn and Se have been associated with adverse clinical outcomes during HIV infection, and new studies are emerging which suggest that micronutrient supplementation may help reduce morbidity and mortality during HIV infection.

scholarly journals Zone-specific damage of the olfactory epithelium under protein restriction

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ayinuer Tuerdi ◽  
Shu Kikuta ◽  
Makoto Kinoshita ◽  
Teru Kamogashira ◽  
Kenji Kondo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Olfactory Epithelium ◽  
Control Diet ◽  
Intervention Strategy ◽  
Protein Restriction ◽  
Outer Hair Cells ◽  
Basal Cells ◽  
Cell Dynamics ◽  
Age Related ◽  
Positive Effect

AbstractOxidative stress causes tissue damage, affecting age-related pathologies. Protein restriction (PR) provides a powerful intervention strategy for reducing oxidative stress, which may have a positive effect on individual organs. However, it is unknown whether PR intervention influences the olfactory system. Here, we investigated how 10 months of PR could affect the cell dynamics of the olfactory epithelium (OE) in mice. We found that PR reduced age-related loss of outer hair cells in the cochlea, providing preventive effects against age-related hearing loss. In contrast, PR resulted in reduced mature olfactory sensory neurons (OSNs), increased proliferative basal cells, and increased apoptotic OSNs in zone 1 (the only area containing neurons expressing NQO1 [quinone dehydrogenase 1]) of the OE in comparison with animals given a control diet. Substantial oxidative stress occurred in NQO1-positive cells and induced apoptotic OSNs in zone 1. These results indicate that in contrast to the positive effect on the auditory system, PR induces oxidative stress and structurally and functionally negative effects on OSNs in zone 1, which is probably involved in the bioactivation of NQO1.

scholarly journals Oxidative Stress, Glutathione Metabolism, and Liver Regeneration Pathways Are Activated in Hereditary Tyrosinemia Type 1 Mice upon Short-Term Nitisinone Discontinuation

Genes ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 3
Author(s):  
Haaike Colemonts-Vroninks ◽  
Jessie Neuckermans ◽  
Lionel Marcelis ◽  
Paul Claes ◽  
Steven Branson ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Liver Regeneration ◽  
The Body ◽  
Glutathione Metabolism ◽  
Short Term ◽  
Therapeutic Regime ◽  
Tyrosinemia Type 1 ◽  
Hereditary Tyrosinemia

Hereditary tyrosinemia type 1 (HT1) is an inherited condition in which the body is unable to break down the amino acid tyrosine due to mutations in the fumarylacetoacetate hydrolase (FAH) gene, coding for the final enzyme of the tyrosine degradation pathway. As a consequence, HT1 patients accumulate toxic tyrosine derivatives causing severe liver damage. Since its introduction, the drug nitisinone (NTBC) has offered a life-saving treatment that inhibits the upstream enzyme 4-hydroxyphenylpyruvate dioxygenase (HPD), thereby preventing production of downstream toxic metabolites. However, HT1 patients under NTBC therapy remain unable to degrade tyrosine. To control the disease and side-effects of the drug, HT1 patients need to take NTBC as an adjunct to a lifelong tyrosine and phenylalanine restricted diet. As a consequence of this strict therapeutic regime, drug compliance issues can arise with significant influence on patient health. In this study, we investigated the molecular impact of short-term NTBC therapy discontinuation on liver tissue of Fah-deficient mice. We found that after seven days of NTBC withdrawal, molecular pathways related to oxidative stress, glutathione metabolism, and liver regeneration were mostly affected. More specifically, NRF2-mediated oxidative stress response and several toxicological gene classes related to reactive oxygen species metabolism were significantly modulated. We observed that the expression of several key glutathione metabolism related genes including Slc7a11 and Ggt1 was highly increased after short-term NTBC therapy deprivation. This stress response was associated with the transcriptional activation of several markers of liver progenitor cells including Atf3, Cyr61, Ddr1, Epcam, Elovl7, and Glis3, indicating a concreted activation of liver regeneration early after NTBC withdrawal.

Short-term high-fat feeding exacerbates degeneration in retinitis pigmentosa by promoting retinal oxidative stress and inflammation

2021 ◽  
Vol 118 (43) ◽  
pp. e2100566118
Author(s):  
Oksana Kutsyr ◽  
Agustina Noailles ◽  
Natalia Martínez-Gil ◽  
Lucía Maestre-Carballa ◽  
Manuel Martinez-Garcia ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Retinitis Pigmentosa ◽  
Retinal Degeneration ◽  
Gut Microbiome ◽  
Cell Activation ◽  
High Fat ◽  
Short Term ◽  
Oxidative Markers ◽  
Degenerative Disorders ◽  
Inflammatory State

A high-fat diet (HFD) can induce hyperglycemia and metabolic syndromes that, in turn, can trigger visual impairment. To evaluate the acute effects of HFD feeding on retinal degeneration, we assessed retinal function and morphology, inflammatory state, oxidative stress, and gut microbiome in dystrophic retinal degeneration 10 (rd10) mice, a model of retinitis pigmentosa, fed an HFD for 2 to 3 wk. Short-term HFD feeding impaired retinal responsiveness and visual acuity and enhanced photoreceptor degeneration, microglial cell activation, and Müller cell gliosis. HFD consumption also triggered the expression of inflammatory and oxidative markers in rd10 retinas. Finally, an HFD caused gut microbiome dysbiosis, increasing the abundance of potentially proinflammatory bacteria. Thus, HFD feeding drives the pathological processes of retinal degeneration by promoting oxidative stress and activating inflammatory-related pathways. Our findings suggest that consumption of an HFD could accelerate the progression of the disease in patients with retinal degenerative disorders.

Failure of Implantation in IVF due to oxidative stress

2021 ◽  
Vol 20 (2) ◽  
pp. 45-52
Author(s):  
Sofoklis Stavros ◽  
Antonios Koutras ◽  
Thomas Ntounis ◽  
Konstantinos Koukoubanis ◽  
Theodoros Papalios ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Reproductive Tract ◽  
Sperm Quality ◽  
Reactive Oxygen ◽  
Female Reproductive Tract ◽  
Oxygen Species ◽  
Stress Markers ◽  
Multiple Levels ◽  
Antioxidant Mechanisms

Oxidative stress may play a role in implantation failure on multiple levels. Oxidative stress is found widely in several biological systems, as well as it acts on various molecular levels with different mechanisms. It has been shown that it is rather the disequilibrium between reactive oxygen species causing oxidative stress and antioxidant mechanisms counteracting their effects, than reactive oxygen species levels themselves. Reactive oxygen species play a role in implantation and fertilisation by acting on different levels of embryo-formation and endometrial changes. Additionally, it is widely abundant in the female reproductive tract including ovaries, oocytes, tubal as well as follicular fluid. Moreover, it has been shown that male fertility is affected by reactive oxygen species by determining sperm quality. Last but not least, oxidative stress may affect IVF indirectly through its actions on peritoneal fluid. As long as research studies on elucidating the development of oxidative stress markers on patients undergoing IVF continue, ever more new possibilities emerge on predicting the pregnancy outcome.

SHORT-TERM RESPONSES TO CADMIUM EXPOSURE IN THE ESTUARINE POLYCHAETE LAEONEREIS ACUTA (POLYCHAETA, NEREIDIDAE): SUBCELLULAR DISTRIBUTION AND OXIDATIVE STRESS GENERATION

2006 ◽  
Vol 25 (5) ◽  
pp. 1337 ◽  
Author(s):  
Juliana Zomer Sandrini ◽  
Francesco Regoli ◽  
Daniele Fattorini ◽  
Alessandra Notti ◽  
Alan Ferreira Inácio ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Subcellular Distribution ◽  
Cadmium Exposure ◽  
Stress Generation ◽  
Short Term ◽  
And Oxidative Stress

scholarly journals The effects of low-dose gamma-irradiation on the wholesomeness of mangoes (Mangifera indica) as determined by short-term feeding studies using rats

1976 ◽  
Vol 35 (1) ◽  
pp. 67-75
Author(s):  
G. M. J. Horton
Keyword(s):  
Mangifera Indica ◽  
Control Diet ◽  
Blood Chemistry ◽  
Female Rats ◽  
Toxic Substances ◽  
Weight Changes ◽  
Short Term ◽  
Dry Matter Digestibility ◽  
Daily Body Weight ◽  
Mango Pulp

1. A control diet and diets containing 150 g non-irradiated or 150 g irradiated mangopulp/kg were given to female rats from day 15 of the gestation period until weaning in trials 1 and 2, and from 40 d before mating until 28 d post weaning in trial 3.2. Food intake and dry-matter digestibility were similar with all diets.3. There were no significant differences between animals given the different dietary regimens in the daily body-weight changes of weanling males, pups, nursing females or females during the immediate postlactation period.4. No differences in haematological or blood chemistry values were found which could be attributed to the ingestion of irradiated mangoes. There was no evidence for the presence of any toxic substances in the irradiated-mango-pulp diet.5. Gross pathological observations revealed no aberrations which could be related to the ingestion of irradiated mangoes.6. It may be concluded that the wholesomeness of mangoes was not affected by gammairradiation at a dose of 75 krd.

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