scholarly journals Effects of Moderate Chronic Food Restriction on the Development of Postprandial Dyslipidemia with Ageing

Nutrients ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 1865 ◽  
Author(s):  
Alejandro Fernández ◽  
Lorena Mazuecos ◽  
Cristina Pintado ◽  
Blanca Rubio ◽  
Virginia López ◽  
...  
Keyword(s):  
Food Restriction ◽  
Wistar Rats ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Serum Glucose ◽  
Loading Test ◽  
Glucose Levels ◽  
Postprandial Triglyceride ◽  
Old Rats ◽  
Chronic Food Restriction

Ageing is a major risk factor for the development of metabolic disorders linked to dyslipidemia, usually accompanied by increased adiposity. The goal of this work was to investigate whether avoiding an excessive increase in adiposity with ageing, via moderate chronic food restriction (FR), ameliorates postprandial dyslipidemia in a rat model of metabolic syndrome associated with ageing. Accordingly, we performed an oral lipid loading test (OLLT) in mature middle-aged (7 months) and middle-old-aged (24 months) Wistar rats fed ad libitum (AL) or under moderate FR for 3 months. Briefly, overnight fasted rats were orally administered a bolus of extra-virgin olive oil (1 mL/Kg of body weight) and blood samples were taken from the tail vein before fat load (t = 0) and 30, 60, 90, 120, 180, and 240 min after fat administration. Changes in serum lipids, glucose, insulin, and glucagon levels were measured at different time-points. Expression of liver and adipose tissue metabolic genes were also determined before (t = 0) and after the fat load (t = 240 min). Postprandial dyslipidemia progressively increased with ageing and this could be associated with hepatic ChREBP activity. Interestingly, moderate chronic FR reduced adiposity and avoided excessive postprandial hypertriglyceridemia in 7- and 24-month-old Wistar rats, strengthening the association between postprandial triglyceride levels and adiposity. The 24-month-old rats needed more insulin to maintain postprandial normoglycemia; nevertheless, hyperglycemia occurred at 240 min after fat administration. FR did not alter the fasted serum glucose levels but it markedly decreased glucagon excursion during the OLLT and the postprandial rise of glycemia in the 24-month-old rats, and FGF21 in the 7-month-old Wistar rats. Hence, our results pointed to an important role of FR in postprandial energy metabolism and insulin resistance in ageing. Lastly, our data support the idea that the vWAT might function as an ectopic site for fat deposition in 7-month-old and in 24-month-old Wistar rats that could increase their browning capacity in response to an acute fat load.

Food restriction prevents age-related polyuria by vasopressin-dependent recruitment of aquaporin-2

2001 ◽  
Vol 281 (6) ◽  
pp. F1123-F1131 ◽  
Author(s):  
S. Combet ◽  
L. Teillet ◽  
G. Geelen ◽  
B. Pitrat ◽  
R. Gobin ◽  
...  
Keyword(s):  
Food Restriction ◽  
Marked Decrease ◽  
Collecting Duct ◽  
Apical Region ◽  
Principal Cells ◽  
Aquaporin 2 ◽  
Age Related ◽  
Old Rats ◽  
Ad Libitum ◽  
Chronic Food Restriction

First published August 8, 2001; 10.1152/ajprenal.00139.2001.—The mechanisms underlying the prevention of age-related polyuria by chronic food restriction were investigated in female WAG/Rij rats. The decreased osmolality of renal papilla observed in senescent rats was not corrected by food restriction. A reduced urea content in the inner medulla of senescent rats, fed ad libitum or food-restricted, was suggested by the marked decrease in expression of UT-A1 and UT-B1 urea transporters. Aquaporin-2 (AQP2) downregulation in the inner medulla of senescent rats was partially prevented by food restriction. Both AQP2 and the phosphorylated form of AQP2 (p-AQP2), the presence of which was diffuse within the cytoplasm of collecting duct principal cells in normally fed senescent rats, were preferentially targeted at the apical region of the cells in food-restricted senescent animals. Plasma vasopressin (AVP) was similar in 10- and 30-mo-old rats fed ad libitum, but was doubled in food-restricted 30-mo-old rats. This study indicates that 1) kidney aging is associated with a marked decrease in AQP2, UT-A1, and UT-B1 expression in the inner medulla and a reduced papillary osmolality; and 2) the prevention of age-related polyuria by chronic food restriction occurs through an improved recruitment of AQP2 and p-AQP2 to the apical membrane in inner medulla principal cells, permitted by increased plasma AVP concentration.

scholarly journals EXTRA VIRGIN OLIVE OIL EFFECT IN THE COMBINATION OF ALCOHOL AND BENZENE ON THE KIDNEY OF RATTUS NOVERGICUS

2021 ◽  
pp. 19-22
Author(s):  
ADETUNJI OPEYEMI ADEBOLA ◽  
ADETUNJI OLUWASEYI ADEGOKE ◽  
NWOBI JOSEPH CHIGBOGU ◽  
ADETUNJI IYABODE TOYIN ◽  
OGUNBIYI BABAFEMI TOSIN ◽  
...  
Keyword(s):  
Olive Oil ◽  
Wistar Rats ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Histological Evidence ◽  
Relative Organ Weight ◽  
Female Wistar Rats ◽  
Cervical Dislocation ◽  
Histological Processing ◽  
Test Level

Objective: This study investigated the use of extra virgin olive oil (EVOO), which is said to possess anti-inflammatory components as an ameliorative substance for the induced inflammation. Methods: Forty-eight female Wistar rats were used for this study. They were divided into eight (1–8) groups (n=6); Control, Ethanol, Benzene, E+B, E+O, B+O, E+B+O with the administration of 25% Ethanol, 200 mg/kg/b.w Benzene, 2 ml (O) EVOO in respect to the tagged grouping names. Animals were euthanized through cervical dislocation after the last day of administration and the liver was excised and part was fixed in formalin solution of 10% for histological processing and the other part was homogenized for biochemical assay in phosphate buffer before centrifugation. Results: Histological evidence creates an understanding of the nephrons in which benzene and ethanol manifest likewise the mitigated ability of EVOO. The relative organ weight provides information on the extent of the damage on nephrons. The kidney test level is significantly expressed. Conclusion: Ethanol and benzene in combination cause serious damage; also, they separately induce nephrons dysfunction. EVOO was shown to mitigate these nephrons damages.

scholarly journals Food Restriction Prevents Advanced Glycation End Product Accumulation and Retards Kidney Aging in Lean Rats

2000 ◽  
Vol 11 (8) ◽  
pp. 1488-1497
Author(s):  
LAURENT TEILLET ◽  
PHILIPPE VERBEKE ◽  
SABINE GOURAUD ◽  
HILAIRE BAKALA ◽  
CAROLINE BOROT-LALOI ◽  
...  
Keyword(s):  
Food Restriction ◽  
Structure And Function ◽  
Female Rats ◽  
Advanced Glycation ◽  
Age Related ◽  
Glycation End Products ◽  
Old Rats ◽  
Cardiovascular Systems ◽  
And Function ◽  
Chronic Food Restriction

Abstract.Tissue content of advanced glycation end products (AGE) increases with age and contributes to the changes in structure and function of the renal and cardiovascular systems. The effect of chronic food restriction on this AGE accumulation was investigated in lean WAG/Rij rats. A 30% food restriction performed from 10 to 30 mo in female rats reduced their mean body weight from 240 ± 7 to 160 ± 12 g, but did not modify their survival. AGE collagen content increased from 14.3 ± 5.5 to 104.7 ± 13.0 arbitrary units per microgram (AU/μg) of hydroxyproline (OHPro) in kidney between 10 and 30 mo, and from 9.7 ± 1.2 to 310.6 ± 34.6 AU/μg OHPro in the abdominal aorta. Food restriction reduced AGE accumulation to 21.4 ± 3.3 and 74.6 ± 16.5 AU/μg OHPro in kidney and aorta of 30-mo-old animals. Similar results were found for collagen prepared from isolated glomeruli (7.8 ± 1.2, 81.2 ± 16.1, and 10.3 ± 4.3 AU/μg OHPro in 10-mo, 30-mo, and restricted 30-mo-old rats). Reduction of intrarenal and arterial AGE accumulation by food restriction was confirmed by immunostaining in optical microscopy. Age-related changes in arterial and kidney structures as polyuria and proteinuria were mainly prevented by food restriction. These data indicate that chronic food restriction reduces the accumulation of AGE and preserves the structure and function of the renal and cardiovascular systems in learn rats, although it did not affect survival of the animals between 10 and 30 mo.

scholarly journals Alleviation of heat-stress-related physiological perturbations in growing rabbits using natural antioxidants

2018 ◽  
Vol 16 (3) ◽  
pp. e0610 ◽  
Author(s):  
Ahmed H. Daader ◽  
Adham A. Al-Sagheer ◽  
Hassan A. Gabr ◽  
Elham A. Abd El-Moniem
Keyword(s):  
Virgin Olive Oil ◽  
Insulin Level ◽  
Natural Antioxidants ◽  
Extra Virgin Olive Oil ◽  
Feed Additives ◽  
Control Group ◽  
Stress Hormone ◽  
Glucose Levels ◽  
Hormone Balance ◽  
Lemongrass Essential Oil

The current study was performed to evaluate the effects of the dietary inclusion of extra virgin olive oil (EVOO), betaine (BET), lemongrass essential oil (LGEO), gallic acid (GA), vitamin C (VC) and vitamin E (VE) on different body temperature traits and stress hormone and glucose levels in heat-stressed growing rabbits. Rabbits were fed diets with no supplementation (control group) or supplemented with 15 g of EVOO, 400 mg of LGEO, 500 mg of GA, 1000 mg of BET, 500 mg of VC, or 200 mg of VE per kg of diet. All tested feed additives, especially EVOO, had a lowering effect on various rabbit temperature traits. Both triiodothyronine (T3) and tetraiodothyronine/thyroxine (T4) were increased (p<0.05) by the addition of BET, VC, EVOO, and VE. With the exception of the VC group, all dietary groups showed no significant changes in the insulin level compared to the control group level. In contrast, the cortisol and glucose levels were diminished (p<0.05) in all treated groups compared to the control levels. The results suggested that all tested supplementations had positive ameliorating effects on growing rabbits under a severe heat load in terms of lowered body temperatures and a favourable stress hormone balance, with the most favourable results found in the EVOO, VC, and BET supplementation groups.

scholarly journals Impaired Central Insulin Response in Aged Wistar Rats: Role of Adiposity

Endocrinology ◽  
2007 ◽  
Vol 148 (11) ◽  
pp. 5238-5247 ◽  
Author(s):  
Miriam García-San Frutos ◽  
Teresa Fernández-Agulló ◽  
Alain J. De Solís ◽  
Antonio Andrés ◽  
Carmen Arribas ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Signal Transduction ◽  
Body Weight ◽  
Food Intake ◽  
Insulin Receptor ◽  
Food Restriction ◽  
Wistar Rats ◽  
Insulin Response ◽  
Old Rats

Insulin, like leptin, is considered as a lipostatic signal acting at a central level. Aging and age-associated adiposity have been related to the development of leptin resistance in Wistar rats. In the present article, hypothalamic insulin response during aging has been studied in Wistar rats. Thus, the effects of intracerebroventricular infusion of insulin during a week on food intake and body weight as well as insulin signal transduction after acute intracerebroventricular insulin administration have been studied in 3-, 8-, and 24-month-old rats. To explore the possible role of age-associated adiposity, these experiments were also performed in 8- and 24-month-old rats after 3 months of food restriction to reduce visceral adiposity index to values below those of young animals. Intracerebroventricular administration of insulin during a week was more efficient at reducing food intake and body weight in 3-month-old rats than in 8- and 24-month-old rats. Hypothalamic insulin-stimulated insulin receptor, GSK3, AKT, and p70S6K phosphorylation decreased with aging. Insulin receptor and IRS-2 phosphoserine was increased in 24-month-old rats. Food restriction improved both insulin responsiveness and insulin signaling. These data suggest that Wistar rats develop hypothalamic insulin resistance with aging. This can be explained by alterations of the signal transduction pathway. The fact that food restriction improves central insulin response and signal transduction points to the age-associated adiposity as a key player in the development of central insulin resistance.

scholarly journals The effect of chronic food restriction on liver acute phase protein response in female and male Wistar rats

2004 ◽  
Vol 54 (1) ◽  
pp. 13-20 ◽  
Author(s):  
Grigorov Ilijana ◽  
Milosavljevic Tanja ◽  
Ilic Mirka ◽  
Bogojevic Desanka ◽  
Ivanovic-Matic Svetlana ◽  
...  
Keyword(s):  
Acute Phase ◽  
Food Restriction ◽  
Wistar Rats ◽  
Acute Phase Protein ◽  
Acute Phase Protein Response ◽  
Phase Protein ◽  
Male Wistar Rats ◽  
Protein Response ◽  
Chronic Food Restriction

Light and electron microscopic investigation of adenohypophyses of germfree, food-restricted, and germfree-food restricted aging, male lobund-wistar rats

1988 ◽  
Vol 46 ◽  
pp. 352-353
Author(s):  
G. Ilse ◽  
K. Kovacs ◽  
N. Ryan ◽  
T. Sano ◽  
L. Stefaneanu ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Microscopic Investigation ◽  
Electron Microscopic Investigation ◽  
Aging Male ◽  
Sprague Dawley ◽  
Electron Microscopic ◽  
Sprague Dawley Rats ◽  
Old Rats ◽  
Ad Libitum ◽  
Microscopic Findings

Germfree state and food restriction have been shown to increase life span and delay tumor occurrence in rats. We report here the histologic, immunocytochemical and electron microscopic findings of adenohypophyses of aging, male Lobund-Wistar rats raised at Lobund Laboratories. In our previous study, the morphologic changes in the adenohypophyses of old rats have been extensively investigated by histology, immunocytochemistry and electron microscopy. Lactotroph adenomas were frequent in Long-Evans and Sprague-Dawley rats, whereas gonadotroph adenomas were frequent in Sprague-Dawley and Wistar rats.Male Lobund-Wistar rats were divided into four groups: 1) conventional, which were raised under normal non-germfree environment and received food ad libitum; 2) germfree-food ad libitum; 3) conventional environment-food restricted and 4) germfree-food restricted. The adenohypophyses were removed from 6-month-, 18-month- and 30-month-old rats. For light microscopy, adenohypophyses were fixed in formalin and embedded in paraffin.

The use of software packages of R factoextra and FactoMineR and their application in principal component analysis for authentication of oils

2020 ◽  
pp. 1
Author(s):  
Irnawati Irnawati ◽  
Florentinus Dika Octa Riswanto ◽  
Sugeng Riyanto ◽  
Sudibyo Martono ◽  
Abdul Rohman
Keyword(s):  
Principal Component Analysis ◽  
Human Life ◽  
Virgin Olive Oil ◽  
Principal Component ◽  
Extra Virgin Olive Oil ◽  
Component Analysis ◽  
R Software ◽  
Software Packages ◽  
Similar Chemical ◽  
Potential Benefits

Several oils have been reported as nutritional source and providing potential benefits for human life. Oil adulteration becomes major issue due to economical attempt to reduce the price of high cost oils. The employment of FTIR spectroscopy combined with Principal Component Analysis (PCA) technique can be applied in oils authentication study. Two of R software packages namely factoextra and FactoMineR were exploited to perform PCA for analysis sixteen various oils from market in Yogyakarta, Indonesia. The results showed that PCA model have been successfully generated using these two statistical packages. Individual plot, variable plot, and biplot were presented to visualize the PCA model. It was also proved that extra virgin olive oil (EVOO) has similar chemical characteristics to palm oil (PO) as reported in the previous study.

Sign in / Sign up

Export Citation Format

Share Document