scholarly journals Protein Hydrolysates from Fenugreek (Trigonella foenum graecum) as Nutraceutical Molecules in Colon Cancer Treatment

Nutrients ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 724 ◽  
Author(s):  
Amine Allaoui ◽  
Sonia Gascón ◽  
Souhila Benomar ◽  
Javier Quero ◽  
Jesús Osada ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Treatment ◽  
Colorectal Adenocarcinoma ◽  
Chemical Compounds ◽  
Protein Hydrolysates ◽  
Cytochrome C Release ◽  
Trigonella Foenum Graecum ◽  
Differentiated Cells ◽  
Cycle Arrest ◽  
Mitochondrial Membrane Permeabilization

The application of plant extracts for therapeutic purposes has been used in traditional medicine since the plants are a source of a great variety of chemical compounds that possess biological activity. Actually, the effect of these extracts on diseases such as cancer is being widely studied. Colorectal adenocarcinoma is one of the main causes of cancer related to death and the second most prevalent carcinoma in Western countries. The aim of this work is to study the possible effect of two fenugreek (Trigonella foenum graecum) protein hydrolysates on treatment and progression of colorectal cancer. Fenugreek proteins from seeds were hydrolysed by using two enzymes separately, which are named Purafect and Esperase, and were then tested on differentiated and undifferentiated human colonic adenocarcinoma Caco2/TC7 cells. Both hydrolysates did not affect the growth of differentiated cells, while they caused a decrease in undifferentiated cell proliferation by early apoptosis and cell cycle arrest in phase G1. This was triggered by a mitochondrial membrane permeabilization, cytochrome C release to cytoplasm, and caspase-3 activation. In addition, the hydrolysates of fenugreek proteins displayed antioxidant activity since they reduce the intracellular levels of ROS. These findings suggest that fenugreek protein hydrolysates could be used as nutraceutical molecules in colorectal cancer treatment.

The Two Mono-Carbonyl Curcumin Analogs, PGV-1 and CCA-1.1: The Chemopreventive Activity Against DMH-Induced Colorectal Cancer Rat and Proteins Target Candidate Involved

2021 ◽  
Author(s):  
Febri Wulandari ◽  
Muthi' Ikawati ◽  
Mitsunori Kirihata ◽  
Sitarina Widyarini ◽  
Jun-Ya Kato ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cell ◽  
Colorectal Adenocarcinoma ◽  
Bioinformatics Analysis ◽  
Colorectal Carcinogenesis ◽  
Curcumin Analogs ◽  
Cycle Arrest ◽  
Cancer Agent ◽  
Wbc Count ◽  
Chemopreventive Activity

Abstract Pentagamavunone-1 and its newest derivatives, CCA-1.1, possess an outstanding cytotoxic activity against several cancer cell lines, especially colorectal cancer. We are continuing to explore the anti-colorectal cancer properties of PGV-1 and CCA-1.1 against DMH-induced colorectal cancer rats and expound the potential protein target in colorectal adenocarcinoma. We utilized DMH 60 mg/kg (subcutaneous injection once a week for 16 weeks) to induce colorectal cancer. PGV-1 and CCA-1.1 at 10 and 20 mg/kg (orally twice a week for 16 weeks) were co-administered with DMH. The WBC count increased in a single DMH group and was countered by co-administration of PGV-1 and CCA-1.1, but no significant differences in RBC. Single DMH treatment for 16 weeks resulted in 80% adenocarcinoma. In contrast, co-administration with PGV-1 and CCA-1.1 suppressed most of the carcinogenic characteristics and symptoms of the pre-malignancy condition. Furthermore, bioinformatics analysis showed that CCA-1.1 has more specific targets than PGV-1, including CDK1, CDK2, MMP3, MMP14, and CYP3A4, which regulate the cell cycle arrest, cancer cell migration, and xenobiotic metabolism, respectively. Interestingly, CCA-1.1 targets CYP3A4, which possibly interrupts DMH metabolism and prevents the initiation of DMH-colorectal carcinogenesis. In conclusion, CCA-1.1 performed better chemopreventive effects against DMH colorectal cancer and might replace PGV-1 to be promoted as a more effective anti-colorectal cancer agent.

scholarly journals Bergenin suppresses the growth of colorectal cancer cells by inhibiting PI3K/AKT/mTOR signaling pathway

2017 ◽  
Vol 16 (10) ◽  
pp. 2307-2313
Author(s):  
Xucan Gao ◽  
Yazhu Wang ◽  
Jianzhuo Zhang ◽  
Lifen Lin ◽  
Qi Yao ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Reactive Oxygen Species ◽  
Cell Cycle ◽  
Dna Damage ◽  
Cell Cycle Arrest ◽  
Colorectal Adenocarcinoma ◽  
G1 Phase ◽  
Oxygen Species ◽  
Cycle Arrest ◽  
Hct116 Cells

Purpose: To investigate anticancer effects of bergenin on human colorectal cancer cell lines.Methods: Human colorectal adenocarcinoma cell line HCT116 was treated with various concentrations of bergenin for 24 and 48 h. Cell viability, apoptosis, cell cycle arrest and reactive oxygen species (ROS) level were analyzed by MTT, flow cytometry and fluorescent dye assays, respectively. DNA damage-associated protein expressions were analyzed by Western blotting.Results: Bergenin significantly suppressed the viability of HCT116 cells. Moreover, bergenin induced cells to accumulate in G1 phase and resulted in DNA breaks in HCT116 cells. It also led to marked accumulation of intracellular reactive oxygen species (ROS), a breaker of DNA strand in HCT116 cells. Interestingly, bergenin inhibited PI3K/AKT/mTOR pathway.Conclusion: Bergenin effectively suppresses the growth of colorectal  adenocarcinoma by inducing generation of intracellular ROS, DNA damage and consequent G1 phase arrest via inhibition of PI3K/AKT/mTOR pathway.Keywords: Bergenin, Colorectal cancer, DNA damage, Cell cycle arrest,  PI3K/AKT/mTOR

scholarly journals Frailty in Patients Undergoing Colorectal Cancer Treatment

2019 ◽  
Vol 33 (6) ◽  
pp. 551-552
Author(s):  
Mateusz Rubinkiewicz ◽  
Michał Pędziwiatr
Keyword(s):  
Colorectal Cancer ◽  
Cancer Treatment ◽  
Colorectal Cancer Treatment

scholarly journals Extract of Pogostemon cablin Possesses Potent Anticancer Activity against Colorectal Cancer Cells In Vitro and In Vivo

2020 ◽  
Vol 2020 ◽  
pp. 1-11 ◽  
Author(s):  
Ju-Huei Chien ◽  
Shan-Chih Lee ◽  
Kai-Fu Chang ◽  
Xiao-Fan Huang ◽  
Yi-Ting Chen ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Apoptosis ◽  
Cycle Arrest ◽  
Anticancer Effects ◽  
Pogostemon Cablin ◽  
Colorectal Cancer Cells ◽  
Potential Applicability ◽  
Cancer Suppression

Pogostemon cablin (PCa), an herb used in traditional Chinese medicine, is routinely used in the amelioration of different types of gastrointestinal discomfort. However, the mechanisms underlying the cancer suppression activity of PCa in colorectal cancer (CRC) cells have yet to be clarified. The aim of this study was to investigate the anticancer effects of PCa, specifically the induction of apoptosis in CRC cells. The growth inhibition curve of CRC cells following exposure to PCa was detected by an MTT assay. Moreover, PCa combined with 5-FU revealed a synergic effect of decreased cell viability. PCa inhibited cell proliferation and induced cell cycle arrest at the G0/G1 phase and cell apoptosis through regulation of associated protein expression. An in vivo study showed that PCa suppressed the growth of CRC via induction of cell apoptosis with no significant change in body weight or organ histology. Our results demonstrated that PCa inhibits the growth of CRC cells and induces apoptosis in vitro and in vivo, which suggests the potential applicability of PCa as an anticancer agent.

Targeting ribosomal G-quadruplexes with naphthalene-diimides as RNA polymerase I inhibitors for colorectal cancer treatment

2021 ◽  
Author(s):  
Victoria Sanchez-Martin ◽  
David A. Schneider ◽  
Matilde Ortiz-Gonzalez ◽  
Ana Soriano-Lerma ◽  
Angel Linde-Rodriguez ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Rna Polymerase ◽  
Cancer Treatment ◽  
Rna Polymerase I ◽  
Polymerase I ◽  
Naphthalene Diimides ◽  
Colorectal Cancer Treatment

scholarly journals Editorial for the Special Issue “Molecular Biomarkers in Colorectal Adenocarcinoma”

2021 ◽  
Vol 22 (4) ◽  
pp. 2052
Author(s):  
Pinelopi I. Artemaki ◽  
Christos K. Kontos
Keyword(s):  
Colorectal Cancer ◽  
Mortality Rate ◽  
Colorectal Adenocarcinoma ◽  
Molecular Biomarkers ◽  
Special Issue

Colorectal cancer (CRC) is one of the most common malignancies, with an elevated mortality rate [...]

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