scholarly journals Key Genes of Lipid Metabolism and WNT-Signaling Are Downregulated in Subcutaneous Adipose Tissue with Moderate Weight Loss

Nutrients ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 639
Author(s):  
Ruth Schübel ◽  
Disorn Sookthai ◽  
Judith Greimel ◽  
Theron Johnson ◽  
Mirja Grafetstätter ◽  
...  
Keyword(s):  
Weight Loss ◽  
Adipose Tissue ◽  
Lipid Metabolism ◽  
Wnt Signaling ◽  
Subcutaneous Adipose Tissue ◽  
Metabolic Effects ◽  
Average Weight ◽  
Expression Of Genes ◽  
Post Hoc ◽  
Subcutaneous Adipose

Smaller cross-sectional studies and bariatric surgery trials suggest that weight loss may change the expression of genes in adipose tissue that have been implicated in the development of metabolic diseases, but well-powered intervention trials are lacking. In post hoc analyses of data from a 12-week dietary intervention trial initially designed to compare metabolic effects of intermittent vs. continuous calorie restriction, we analyzed the effects of overall weight loss on the subcutaneous adipose tissue (SAT) transcriptome. Changes in the transcriptome were measured by microarray using SAT samples of 138 overweight or obese individuals (age range: 35–65 years, BMI range: 25–40, non-smokers, non-diabetics). Participants were grouped post hoc according to the degree of their weight loss by quartiles (average weight loss in quartiles 1 to 4: 0%, −3.2%, −5.9%, and −10.7%). Candidate genes showing differential expression with weight loss according to microarray analyses were validated by reverse transcription quantitative polymerase chain reaction (RT-qPCR), and fold changes (FCs) were calculated to quantify differences in gene expression. A comparison of individuals in the highest vs. the lowest weight loss quartile revealed 681 genes to be differentially expressed (corrected p < 0.05), with 40 showing FCs of at least 0.4. Out of these, expression changes in secreted frizzled-related protein 2 (SFRP2, FC = 0.65, p = 0.006), stearoyl-CoA desaturase (SCD, FC = −1.00, p < 0.001), and hypoxia inducible lipid droplet-associated (HILPDA, FC = −0.45, p = 0.001) with weight loss were confirmed by RT-qPCR. Dietary weight loss induces significant changes in the expression of genes implicated in lipid metabolism (SCD and HILPDA) and WNT-signaling (SFRP2) in SAT.

Insulin resistance, systemic and regional inflammation in subcutaneous adipose tissue during weight loss in a population with obesity

2019 ◽  
Author(s):  
Frederique Van de Velde ◽  
Margriet Ouwens ◽  
Arsene-Helene Batens ◽  
Samyah Shadid ◽  
Bruno Lapauw ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Weight Loss ◽  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Subcutaneous Adipose

Effect of Roux-en-Y gastric bypass-induced weight loss on the transcriptomic profiling of subcutaneous adipose tissue

2016 ◽  
Vol 12 (2) ◽  
pp. 257-263 ◽  
Author(s):  
Juan José González-Plaza ◽  
Carolina Gutiérrez-Repiso ◽  
Sara García-Serrano ◽  
Francisca Rodriguez-Pacheco ◽  
Lourdes Garrido-Sánchez ◽  
...  
Keyword(s):  
Weight Loss ◽  
Gastric Bypass ◽  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Transcriptomic Profiling ◽  
Subcutaneous Adipose

scholarly journals Assessing the Contribution of Relative Macrophage Frequencies to Subcutaneous Adipose Tissue

2021 ◽  
Vol 8 ◽  
Author(s):  
Marianthi Kalafati ◽  
Michael Lenz ◽  
Gökhan Ertaylan ◽  
Ilja C. W. Arts ◽  
Chris T. Evelo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Tissue Cell ◽  
Cell Type ◽  
Expression Of Genes ◽  
Cell Type Composition ◽  
Type Composition ◽  
Adipose Tissue Cell ◽  
Subcutaneous Adipose

Background: Macrophages play an important role in regulating adipose tissue function, while their frequencies in adipose tissue vary between individuals. Adipose tissue infiltration by high frequencies of macrophages has been linked to changes in adipokine levels and low-grade inflammation, frequently associated with the progression of obesity. The objective of this project was to assess the contribution of relative macrophage frequencies to the overall subcutaneous adipose tissue gene expression using publicly available datasets.Methods: Seven publicly available microarray gene expression datasets from human subcutaneous adipose tissue biopsies (n = 519) were used together with TissueDecoder to determine the adipose tissue cell-type composition of each sample. We divided the subjects in four groups based on their relative macrophage frequencies. Differential gene expression analysis between the high and low relative macrophage frequencies groups was performed, adjusting for sex and study. Finally, biological processes were identified using pathway enrichment and network analysis.Results: We observed lower frequencies of adipocytes and higher frequencies of adipose stem cells in individuals characterized by high macrophage frequencies. We additionally studied whether, within subcutaneous adipose tissue, interindividual differences in the relative frequencies of macrophages were reflected in transcriptional differences in metabolic and inflammatory pathways. Adipose tissue of individuals with high macrophage frequencies had a higher expression of genes involved in complement activation, chemotaxis, focal adhesion, and oxidative stress. Similarly, we observed a lower expression of genes involved in lipid metabolism, fatty acid synthesis, and oxidation and mitochondrial respiration.Conclusion: We present an approach that combines publicly available subcutaneous adipose tissue gene expression datasets with a deconvolution algorithm to calculate subcutaneous adipose tissue cell-type composition. The results showed the expected increased inflammation gene expression profile accompanied by decreased gene expression in pathways related to lipid metabolism and mitochondrial respiration in subcutaneous adipose tissue in individuals characterized by high macrophage frequencies. This approach demonstrates the hidden strength of reusing publicly available data to gain cell-type-specific insights into adipose tissue function.

scholarly journals Increased Subcutaneous Adipose Tissue Expression of Genes Involved in Glycerolipid-Fatty Acid Cycling in Obese Insulin-Resistant Versus -Sensitive Individuals

2014 ◽  
Vol 99 (12) ◽  
pp. E2518-E2528 ◽  
Author(s):  
Marie-Soleil Gauthier ◽  
Joelle R. Pérusse ◽  
Marie-Ève Lavoie ◽  
Robert Sladek ◽  
S. R. Murthy Madiraju ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Subcutaneous Adipose Tissue ◽  
Tissue Expression ◽  
Insulin Resistant ◽  
Expression Of Genes ◽  
Subcutaneous Adipose

scholarly journals Elevated Levels of Interleukin 6 Are Reduced in Serum and Subcutaneous Adipose Tissue of Obese Women after Weight Loss*

2000 ◽  
Vol 85 (9) ◽  
pp. 3338-3342 ◽  
Author(s):  
Jean-Philippe Bastard ◽  
Claude Jardel ◽  
Eric Bruckert ◽  
Patricia Blondy ◽  
Jacqueline Capeau ◽  
...  
Keyword(s):  
Weight Loss ◽  
Adipose Tissue ◽  
Interleukin 6 ◽  
Subcutaneous Adipose Tissue ◽  
Obese Women ◽  
Subcutaneous Adipose
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