scholarly journals Assessment of the Sensitizing Potential of Proteins in BALB/c Mice: Comparison of Three Protocols of Intraperitoneal Sensitization

Nutrients ◽  
2018 ◽  
Vol 10 (7) ◽  
pp. 903 ◽  
Author(s):  
Jesús Arámburo-Galvez ◽  
Norberto Sotelo-Cruz ◽  
Lilian Flores-Mendoza ◽  
Martina Gracia-Valenzuela ◽  
Francisco Chiquete-Elizalde ◽  
...  
Keyword(s):  
Milk Proteins ◽  
Serum Levels ◽  
Specific Ige ◽  
Cow Milk ◽  
Sample Collection ◽  
Igg Antibody ◽  
Allergenic Protein ◽  
Ige Antibodies ◽  
Undetectable Serum ◽  
Specific Ige Antibodies

Most food allergy cases are associated with a limited group of allergens. This could be attributed to an increased ability of some foods to sensitize and trigger allergic reactions. However, there are no validated animal models to evaluate the sensitizing or allergenic potentials of proteins. Our aim was to evaluate three protocols of adjuvant-free intraperitoneal sensitization that differ in the time points for sample collection (days 14, 28 and 35 from beginning of the sensitization) and also in the number of immunizations (2, 5 and 3, respectively). Ovalbumin (OVA; 0.05 mg), cow milk proteins (CMP; 0.025, 0.05 and 0.25 mg), and potato acid phosphatase (PAP; low allergenic protein; 250.0 mg) were administered intraperitoneally (ip) to BALB/c mice (n = 4–6) and the protein-specific IgE and IgG antibody responses were evaluated using ELISA. Additional serum protein-specific IgE antibodies evaluations were carried out after IgG depletion. Anti-OVA IgE antibodies were detected in mice from all three protocols. The responses were higher in the group of mice that underwent the 28-day protocol than in those that underwent the 14- or 35-day protocols (p < 0.01 and p < 0.05, respectively). Anti-CMP IgE antibodies were detected in both the 14- and 28-day protocols, but the response was higher in the group that underwent the 28-day protocol (p < 0.001). The anti-CMP IgE antibody response detection was improved after serum IgG depletion (p < 0.001). Anti-PAP IgE antibodies were not detected. Mice with undetectable serum levels of protein-specific IgE triggered anti-OVA, -CMP, and -PAP IgG responses. An adjuvant-free 28-day protocol with five ip immunizations seems appropriate for evaluation of the inherent sensitizing or allergenic capacity of the studied proteins. Reproducible results were obtained utilizing the BALB/c mouse strain. Inter-laboratory studies including a larger number of proteins should be carried out to validate this model.

scholarly journals CLINICAL AND LABORATORY ASPECTS OF DETECTING SPECIFIC IgE ANTIBODIES TO COW’S MILK AND ITS COMPONENTS

2019 ◽  
Vol 21 (5) ◽  
pp. 937-944
Author(s):  
N. A. Alkhutova ◽  
N. A. Kovyazina ◽  
O. L. Zhizhina
Keyword(s):  
Food Allergy ◽  
Clinical Signs ◽  
Specific Ige ◽  
Infants And Children ◽  
Cow Milk ◽  
School Age ◽  
Milk Allergy ◽  
Ige Antibodies ◽  
Specific Ige Antibodies ◽  
Antibody Levels

Food allergy against cow milk and its components is highly prevalent among infants and children of pre-school and young school age being a sufficient factor influencing health condition of children during the maturation period. Clinical signs of the milk allergy are non-specific, and they may be pronounced or expressed in mild form, thus enabling hypodiagnistics of this disorder. Moreover, a variety of milk antigens determines different clinical course of this allergic condition and brings additional difficulties to its diagnostics and treatment. Meanwhile, a sensibilization for the cow milk proteins may sometimes trigger a generalized atopy and bronchial asthma, being a factor delayed growth and other health disorders. At the present time, there are no distinct risk criteria for milk allergy. Therefore, its successful prophylaxis and treatment largely depends on the competence of clinical allergologist and informativity of the diagnostic techniques used. So far, however, we have no generally approved laboratory algorithms for diagnostics and monitoring of treatment efficiency in the cow milk allergy and its compomemts.We have performed a laboratory study of 187 children at the age of 3 months to 10 years. An immunochemoluminescent assay of specific IgE antibody levels to the cow milk using IMMULITE 2000/XPi analyzer has revealed its good informative value at different approaches to prediction and evaluation of food allergy treatment, both oriented for a critical cutoff value of 3 МU/L, and by monitoring a decrease in antibody levels. The authors consider rational an extended indefinity principle during the patient-oriented interpretation of IgE assay results if clinical decision is based on critical value of the index. In cases of clinical monitoring, the limit of interim reproducibility should be taken into account. The prevalence of specific cow milk antibodies among the boys was higher than among girls, however, with lesser frequency of moderate/high reactivity among the males. Moreover. the cases were detected with higher levels of anti-beta-lactalbumin IgG than those against whole milk. This finding should be considered during the screening studies.On the basis of literature analysis and own results, the authors propose an extensive study of specific IgE antibodies against cow milk and its components in blood serum of infants and children form the pre-school and junior school age groups. 

Allergen-Specific IgE Antibodies against Antigenic Components in Cow Milk and Milk Substitutes

Allergy ◽  
1986 ◽  
Vol 41 (1) ◽  
pp. 51-56 ◽  
Author(s):  
B. Gjesing ◽  
O. Østerballe ◽  
B. Schwartz ◽  
U. Wahn ◽  
H. Lowenstein
Keyword(s):  
Specific Ige ◽  
Cow Milk ◽  
Ige Antibodies ◽  
Milk Substitutes ◽  
Specific Ige Antibodies

scholarly journals Staphylococcal superantigen-specific IgE antibodies: degree of sensitization and association with severity of asthma

2016 ◽  
Vol 42 (5) ◽  
pp. 356-361 ◽  
Author(s):  
José Elabras Filho ◽  
Fernanda Carvalho de Queiroz Mello ◽  
Omar Lupi ◽  
Blanca Elena Rios Gomes Bica ◽  
José Angelo de Souza Papi ◽  
...  
Keyword(s):  
Tertiary Care ◽  
Persistent Asthma ◽  
Serum Levels ◽  
Specific Ige ◽  
Clinical Severity ◽  
University Hospital ◽  
Ige Antibodies ◽  
Staphylococcal Toxin ◽  
Quantitative Results ◽  
Specific Ige Antibodies

ABSTRACT Objective: To determine the presence of staphylococcal superantigen-specific IgE antibodies and degree of IgE-mediated sensitization, as well as whether or not those are associated with the severity of asthma in adult patients. Methods: This was a cross-sectional study involving outpatients with asthma under treatment at a tertiary care university hospital in the city of Rio de Janeiro, Brazil. Consecutive patients were divided into two groups according to the severity of asthma based on the Global Initiative for Asthma criteria: mild asthma (MA), comprising patients with mild intermittent or persistent asthma; and moderate or severe asthma (MSA). We determined the serum levels of staphylococcal toxin-specific IgE antibodies, comparing the results and performing a statistical analysis. Results: The study included 142 patients: 72 in the MA group (median age = 46 years; 59 females) and 70 in the MSA group (median age = 56 years; 60 females). In the sample as a whole, 62 patients (43.7%) presented positive results for staphylococcal toxin-specific IgE antibodies: staphylococcal enterotoxin A (SEA), in 29 (20.4%); SEB, in 35 (24.6%); SEC, in 33 (23.2%); and toxic shock syndrome toxin (TSST), in 45 (31.7%). The mean serum levels of IgE antibodies to SEA, SEB, SEC, and TSST were 0.96 U/L, 1.09 U/L, 1.21 U/L, and 1.18 U/L, respectively. There were no statistically significant differences between the two groups in terms of the qualitative or quantitative results. Conclusions: Serum IgE antibodies to SEA, SEB, SEC, and TSST were detected in 43.7% of the patients in our sample. However, neither the qualitative nor quantitative results showed a statistically significant association with the clinical severity of asthma.

In vitro detection of specific IgE antibodies to erythromycin☆☆☆★★★

1995 ◽  
Vol 95 (3) ◽  
pp. 668-671 ◽  
Author(s):  
Cristina Pascual ◽  
Jesus F. Crespo ◽  
Joaquin Quiralte ◽  
Concepcion Lopez ◽  
Gary Wheeler ◽  
...  
Keyword(s):  
Specific Ige ◽  
Ige Antibodies ◽  
Specific Ige Antibodies

scholarly journals Peanut-specific IgE antibodies in asymptomatic Ghanaian children possibly caused by carbohydrate determinant cross-reactivity

2013 ◽  
Vol 132 (3) ◽  
pp. 639-647 ◽  
Author(s):  
Abena S. Amoah ◽  
Benedicta B. Obeng ◽  
Irene A. Larbi ◽  
Serge A. Versteeg ◽  
Yvonne Aryeetey ◽  
...  
Keyword(s):  
Specific Ige ◽  
Cross Reactivity ◽  
Ige Antibodies ◽  
Specific Ige Antibodies

Serum IgE Levels and Specific IgE Antibodies in House Dust Mite Allergy: Predictive Value

1983 ◽  
Vol 20 (2) ◽  
pp. 93-96 ◽  
Author(s):  
LF. Perrin ◽  
J. Sroussi ◽  
F Cea-Gil ◽  
P. Deviller ◽  
Y. Lasne
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Predictive Value ◽  
Specific Ige ◽  
Ige Antibodies ◽  
Serum Ige ◽  
House Dust Mite Allergy ◽  
Mite Allergy ◽  
Dust Mite ◽  
Specific Ige Antibodies
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