scholarly journals NanoTiO2 Sunscreen Does Not Prevent Systemic Oxidative Stress Caused by UV Radiation and a Minor Amount of NanoTiO2 is Absorbed in Humans

Nanomaterials ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 888 ◽  
Author(s):  
Daniela Pelclova ◽  
Tomas Navratil ◽  
Tereza Kacerova ◽  
Blanka Zamostna ◽  
Zdenka Fenclova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mass Spectrometry ◽  
Uv Radiation ◽  
Inductively Coupled Plasma ◽  
Exhaled Breath Condensate ◽  
Prostaglandin F2α ◽  
Minor Amount ◽  
Exhaled Breath ◽  
Skin Absorption ◽  
Systemic Oxidative Stress

The present pilot study tested the efficiency of nanoTiO2 sunscreen to prevent the oxidative stress/inflammation caused by ultraviolet (UV) radiation using biomarkers in subjects’ blood, urine, and exhaled breath condensate (EBC). In addition, the skin absorption of nanoTiO2 was studied. Six identical subjects participated in three tests: (A) nanoTiO2 sunscreen, (B) UV radiation, and (C) sunscreen + UV. The first samples were collected before the test and the second after sunscreen application and/or UV exposure. On day 4, the third samples were collected, and the sunscreen was washed off, and the fourth samples were collected on day 11. The following biomarkers were measured: malondialdehyde, 4-hydroxy-trans-hexenal, 4-hydroxy-trans-nonenal, aldehydes C6-C12, 8-iso-Prostaglandin F2α, o-tyrosine, 3-chlorotyrosine, 3-nitrotyrosine, 8-hydroxy-2-deoxyguanosine, 8-hydroxyguanosine, 5-hydroxymethyl uracil, and leukotrienes, using liquid chromatography-electrospray ionisation-tandem mass spectrometry. Titania was measured using inductively coupled plasma mass spectrometry and TiO2 nanoparticles by transmission and scanning electron microscopy. Sunscreen alone did not elevate the markers, but UV increased the biomarkers in the plasma, urine, and EBC. The sunscreen prevented skin redness, however it did not inhibit the elevation of oxidative stress/inflammatory markers. Titania and nanoTiO2 particles were found in the plasma and urine (but not in the EBC) in all sunscreen users, suggesting their skin absorption.

Oxidative Stress Markers In The Exhaled Breath Condensate Of Children With OSA

2011 ◽  
Author(s):  
Athanasios Kaditis ◽  
Georgia Malakasioti ◽  
Emmanouel Alexopoulos ◽  
Vasiliki Varlami ◽  
Christina Befani ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Breath Condensate

scholarly journals Sub-nanoliter metabolomics via mass spectrometry to characterize volume-limited samples

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Yafeng Li ◽  
Marcos Bouza ◽  
Changsheng Wu ◽  
Hengyu Guo ◽  
Danning Huang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Stromal Cells ◽  
Exhaled Breath Condensate ◽  
Sample Volume ◽  
Triboelectric Nanogenerator ◽  
Exhaled Breath ◽  
Nanoelectrospray Ionization ◽  
External Stimuli ◽  
Breath Condensate

Abstract The human metabolome provides a window into the mechanisms and biomarkers of various diseases. However, because of limited availability, many sample types are still difficult to study by metabolomic analyses. Here, we present a mass spectrometry (MS)-based metabolomics strategy that only consumes sub-nanoliter sample volumes. The approach consists of combining a customized metabolomics workflow with a pulsed MS ion generation method, known as triboelectric nanogenerator inductive nanoelectrospray ionization (TENGi nanoESI) MS. Samples tested with this approach include exhaled breath condensate collected from cystic fibrosis patients as well as in vitro-cultured human mesenchymal stromal cells. Both test samples are only available in minimum amounts. Experiments show that picoliter-volume spray pulses suffice to generate high-quality spectral fingerprints, which increase the information density produced per unit sample volume. This TENGi nanoESI strategy has the potential to fill in the gap in metabolomics where liquid chromatography-MS-based analyses cannot be applied. Our method opens up avenues for future investigations into understanding metabolic changes caused by diseases or external stimuli.

Selected ion flow tube mass spectrometry of exhaled breath condensate headspace

2008 ◽  
Vol 22 (18) ◽  
pp. 2844-2850 ◽  
Author(s):  
Petr Čáp ◽  
Kseniya Dryahina ◽  
František Pehal ◽  
Patrik Španěl
Keyword(s):  
Mass Spectrometry ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Flow Tube ◽  
Ion Flow ◽  
Selected Ion Flow Tube ◽  
Breath Condensate
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