scholarly journals Synthesis of Gold Nanoparticles (AuNPs) Using Ricinus communis Leaf Ethanol Extract, Their Characterization, and Biological Applications

Nanomaterials ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 765 ◽  
Author(s):  
Hamed A. Ghramh ◽  
Khalid Ali Khan ◽  
Essam H. Ibrahim ◽  
William N. Setzer
Keyword(s):  
Antimicrobial Activity ◽  
Gold Nanoparticles ◽  
Cell Viability ◽  
Red Blood Cells ◽  
Hela Cells ◽  
Blood Cells ◽  
Hepg2 Cells ◽  
Ricinus Communis ◽  
Inhibitory Effects ◽  
Splenic Cells

The purpose of this study was to explore the collective biological properties of Ricinus communis ethanol leaf extract (RcExt) and extract-fabricated gold nanoparticles (RcExt-AuNPs). AuNPs were synthesized using RcExt. Fingerprint data of the biochemicals putatively found in RcExt were obtained using gas chromatography–mass spectrometry (GC-MS/MS) and high-performance liquid chromatography/ultraviolet-visible (HPLC/UV-VIS) analyses. RcExt-AuNPs were characterized by UV-Vis spectroscopy, scanning electron microscopy (SEM), and Fourier- transform infrared radiation (FTIR) spectroscopy. Cytotoxic activity on the Hela and HepG2 tumor cell lines was tested through cell viability, antimicrobial activity against bacterial and fungal pathogens through a well diffusion assay, hemolytic activity on red blood cells through absorbance reading, and stimulatory/inhibitory effects on splenic cells by cell viability. AuNPs of 200 nm size were synthesized. GC-MS/MS analysis revealed 12 peaks and HPLC/UV-VIS analysis resulted in 18, 13, and five peaks at the wavelengths of 220, 254, and 300 nm, respectively. Cytotoxicity screening revealed that RcExt had stimulatory effects (6.08%) on Hela cells and an inhibitory effect (−28.33%) on HepG2 cells, whereas RcExt-AuNPs showed inhibitory effects (−58.64% and −42.74%) on Hela and HepG2 cells, respectively. Antimicrobial activity of RcExt-AuNPs against tested pathogens was significantly higher (average diameters of inhibition zones were higher (ranging from 9.33 mm to 16.33 mm)) than those of RcExt (ranging from 6.00 mm to 7.33 mm). RcExt and RcExt-AuNPs showed 4.15% and 100% lytic effects, respectively. Inhibitory effects on splenic cells for RcExt-AuNPs were observed to be significantly higher (−30.56% to −72.62%) than those of RcExt (−41.55% to −62.25%) between concentrations of 25 to 200 µg/mL. RcExt-AuNPs were inhibitory against HepG2 and Hela cells, while RcExt inhibited HepG2 but stimulated Hela cells. RcExt-AuNPs showed comparatively more antimicrobial activity. RcExt was safe while RcExt-AuNPs harmful to red blood cells (RBCs). RcExt and RcExt-AuNPs showed inhibitory effects on splenic cells irrespective of dose.

scholarly journals Biological Activities of Euphorbia peplus Leaves Ethanolic Extract and the Extract Fabricated Gold Nanoparticles (AuNPs)

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1431 ◽  
Author(s):  
Hamed A. Ghramh ◽  
Khalid Ali Khan ◽  
Essam H. Ibrahim
Keyword(s):  
Antimicrobial Activity ◽  
Gold Nanoparticles ◽  
Hela Cells ◽  
Hepg2 Cells ◽  
Biological Activities ◽  
Inhibitory Effect ◽  
Mosquito Larvae ◽  
Inhibitory Effects ◽  
Euphorbia Peplus ◽  
Dose Dependent

Euphorbia peplus leaves extract (EpExt) and gold nanoparticles (AuNPs) phytofabricated with extract (EpExt-AuNPs) were investigated for biological activities. EpExt and EpExt-AuNPs were screened for: (i) anticancer activity against Hela and HepG2 cell lines; (ii) antimicrobial activity; (iii) hemolytic activity; (iv) cytotoxic or stimulatory effects; and (v) insecticidal activity. AuNPs (size 50 nm) were synthesized. (i) EpExt had a stimulatory effect (51.04%) on Hela cells and an inhibitory effect (−12.83%) on HepG2 cells while EpExt-AuNPs showed inhibitory effects (−54.25% and −59.64% on Hela and HepG2 cells respectively). (ii) Antimicrobial activity of EpExt-AuNPs was significantly higher (ranged from 11.67 mm to 14.33 mm) than that of EpExt (ranged from 5.33 mm to 6.33 mm). (iii) Both EpExt and EpExt-AuNPs displayed 100% hemolysis. (iv) A dose-dependent inhibitory effect of EpExt was observed (ranged from −48.5% to −92.1%), which was greater than that of EpExt-AuNPs (ranged from −32.1% to −69.1%) (v) EpExt-AuNPs was more lethal against mosquito larvae with lethal concentration (LC50) value (202.692 ppm) compared to EpExt (1430.590 ppm). In conclusion, EpExt-AuNPs were inhibitory against HepG2 and Hela cells, while EpExt inhibited HepG2 but stimulated Hela cells. EpExt-AuNPs had antimicrobial effects. EpExt showed dose-dependent inhibitory effects on splenic cells. EpExt-AuNPs were lethal against mosquito larvae.

scholarly journals Human Leukocyte Antigen and Red Blood Cells Impact Umbilical Cord Blood CD34+ Cell Viability after Thawing

2019 ◽  
Vol 20 (19) ◽  
pp. 4875 ◽  
Author(s):  
Vanegas ◽  
Galindo ◽  
Páez-Gutiérrez ◽  
González-Acero ◽  
Medina-Valderrama ◽  
...  
Keyword(s):  
Human Leukocyte Antigen ◽  
Cell Viability ◽  
Cord Blood ◽  
Red Blood Cells ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Blood Cells ◽  
Human Leukocyte ◽  
Hla Typing ◽  
Leukocyte Antigen

Hematopoietic progenitor cell (HPC) transplantation is a treatment option for malignant and nonmalignant diseases. Umbilical cord blood (UCB) is an important HPC source, mainly for pediatric patients. It has been demonstrated that human leukocyte antigen (HLA) matching and cell dose are the most important features impacting clinical outcomes. However, UCB matching is performed using low resolution HLA typing and it has been demonstrated that the unnoticed mismatches negatively impact the transplant. Since we found differences in CD34+ viability after thawing of UCB units matched for two different patients (p = 0.05), we presumed a possible association between CD34+ cell viability and HLA. We performed a multivariate linear model (n = 67), comprising pre-cryopreservation variables and high resolution HLA genotypes separately. We found that pre-cryopreservation red blood cells (RBC), granulocytes, and viable CD34+ cell count significantly impacted CD34+ viability after thawing, along with HLA-B or -C (R2 = 0.95, p = 0.01; R2 = 0.56, p = 0.007, respectively). Although HLA-B*40:02 may have a negative impact on CD34+ cell viability, RBC depletion significantly improves it.

Biocompatibility of surface-modified gold nanoparticles towards red blood cells and haemoglobin

2020 ◽  
Vol 512 ◽  
pp. 145573 ◽  
Author(s):  
Sumit Kumar ◽  
Indrani Jha ◽  
Navin Kumar Mogha ◽  
Pannuru Venkatesu
Keyword(s):  
Gold Nanoparticles ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Surface Modified

Synthesis and characterisation of glucose-functional glycopolymers and gold nanoparticles: study of their potential interactions with ovine red blood cells

2015 ◽  
Vol 405 ◽  
pp. 47-54 ◽  
Author(s):  
Laura E. Wilkins ◽  
Daniel J. Phillips ◽  
Robert C. Deller ◽  
Gemma-Louise Davies ◽  
Matthew I. Gibson
Keyword(s):  
Gold Nanoparticles ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Potential Interactions

scholarly journals Inhibitory Effects of Triterpenes Isolated from Chuling (Polyporus umbellatus FRIES) on Free Radical-Induced Lysis of Red Blood Cells

2005 ◽  
Vol 28 (5) ◽  
pp. 817-821 ◽  
Author(s):  
Nobuyasu Sekiya ◽  
Hiroaki Hikiami ◽  
Yoichiro Nakai ◽  
Iwao Sakakibara ◽  
Kazuya Nozaki ◽  
...  
Keyword(s):  
Free Radical ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Inhibitory Effects ◽  
Polyporus Umbellatus

Green Synthesis, Structural, In Vitro and In Vivo Bioactivity Properties of ZnO Nanoparticles for Biomedical Applications

2019 ◽  
Vol 9 (6) ◽  
pp. 731-738 ◽  
Author(s):  
Essam H. Ibrahim ◽  
Obaid Albulym ◽  
Omer Kaygili ◽  
Mona Kilany ◽  
Mohd. Shkir ◽  
...  
Keyword(s):  
Green Synthesis ◽  
Red Blood Cells ◽  
Cell Line ◽  
Zno Nanoparticles ◽  
Blood Cells ◽  
Cytotoxicity Test ◽  
Splenic Cells ◽  
Normal Rat

Owing to the fascinating applications of ZnO in modern devices, it is interesting to explore its more aspects for future devices. Hence, herein, we have synthesized the high purity spherical ZnO nanoparticles (SNPs) through a facile green synthesis route and robust structural and biomedical studies are carried out. Hexagonal phase with 93.2% crystallinity was confirmed through XRD analysis. ZnO nanoparticles were tested for their bioactivities both in vivo (acute cytotoxicity test) and in vitro (Anti-cancer activities on the liver (HepG2) and cervical (Hela) cancer cell lines, stimulatory/inhibitory effects on normal rat splenic cells, hemolytic effects on red blood cells and antimicrobial activity). Results showed that ZnO SNPs has no cytotoxic effects on the vital organ like the liver and has no hemolytic action on red blood cells. ZnO SNPs showed inhibitory consequence on normal rat splenic cells growth at all tested concentrations. The ZnO nanoparticles showed a stimulatory effect on Hela cell line. Moreover, ZnO nanoparticles showed an inhibitory effect on HepG2 cell line and microbial cells.

A Novel Nitric Oxide Cellular Biosensor Based on Red Blood Cells Immobilized on Gold Nanoparticles

2006 ◽  
Vol 39 (15) ◽  
pp. 2849-2859 ◽  
Author(s):  
Hai‐Ying Gu ◽  
Shuang‐Yun Lu ◽  
Qi‐Yu Jiang ◽  
Chun‐Mei Yu ◽  
Genxi Li ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Gold Nanoparticles ◽  
Red Blood Cells ◽  
Blood Cells

scholarly journals Effect of the extract of Ricinus communis L. on the osmotic fragility, labeling of red blood cells with Technetium-99m and morphology of the cells

2008 ◽  
Vol 51 (6) ◽  
pp. 1139-1146 ◽  
Author(s):  
Kristiana Cerqueira Mousinho ◽  
Marília Bezerra Libório Correia ◽  
Jailson Oliveira da Silva ◽  
Simey de Souza Leão Pereira Magnata ◽  
Ivone Antônia de Souza ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Ricinus Communis ◽  
Osmotic Fragility ◽  
Insoluble Fraction ◽  
Cell Physiology ◽  
Morphological Alterations ◽  
Ricinus Communis L ◽  
Stannous Ion ◽  
Blood Elements

The aim of this study was to evaluate the influence of the proteic extract of R. communis on the cell physiology by the osmotic fragility, labeling of the blood elements with the 99mTc and cell morphology. To evaluate the osmotic fragility, the blood samples of the Wistar rats were incubated with the concentrations of R. communis and with the solutions of NaCl (0.4; 0.7; 0.9%). In the labeling of the blood elements procedure, the rat blood was treated with a solution of Tc-99m and TCA at 5%, determining the rate of radioactivity (%ATI) in the plasma (P) and in the red blood cells (RBC). The soluble and insoluble fractions of the plasma were also evaluated. The cells morphology submitted to the extract was evaluated by the optical microscopy (x40). The results indicated that the rate of the hemolysis increased in the presence of 0.125 mg/mL of the extract. There was a decay of 49.69% in the rate of ATI in the insoluble fraction of the cells, with the morphological alterations in the red blood cells. These results suggested that the extract changed the capability of binding of the red blood cells due to the stannous ion oxidation, modifying the cells structure.

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