scholarly journals Biomimetic Collagen/Zn2+-Substituted Calcium Phosphate Composite Coatings on Titanium Substrates as Prospective Bioactive Layer for Implants: A Comparative Study Spin Coating vs. MAPLE

Nanomaterials ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 692 ◽  
Author(s):  
Ionela Andreea Neacsu ◽  
Laura Vasilica Arsenie ◽  
Roxana Trusca ◽  
Ioana Lavinia Ardelean ◽  
Natalia Mihailescu ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Comparative Study ◽  
Spin Coating ◽  
Composite Coatings ◽  
Titanium Implants ◽  
Laser Evaporation ◽  
Mineralization Process ◽  
Ftir Microscopy ◽  
Titanium Substrates

Synthesis of biomimetic materials for implants and prostheses is a hot topic in nanobiotechnology strategies. Today the major approach of orthopaedic implants in hard tissue engineering is represented by titanium implants. A comparative study of hybrid thin coatings deposition was performed by spin coating and matrix-assisted pulsed laser evaporation (MAPLE) onto titanium substrates. The Collagen-calcium phosphate (Coll-CaPs) combination was selected as the best option to mimic natural bone tissue. To accelerate the mineralization process, Zn2+ ions were inserted by substitution in CaPs. A superior thin film homogeneity was assessed by MAPLE, as shown by scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) microscopy. A decrease of P-O and amide absorbance bands was observed as a consequence of different Zn2+ amounts. A variety of structural modifications of the apatite layer are then generated, which influenced the confinement process towards the collagen template. The in-vitro Simulated Body Fluid (SBF) assay demonstrated the ability of Coll/Zn2+-CaPs coatings to stimulate the mineralization process as a result of synergic effects in the collagen-Zn2+ substituted apatite. For both deposition methods, the formation of droplets associated to the growth of CaPs particulates inside the collagen matrix was visualized. This supports the prospective behavior of MAPLE biomimetic coatings to induce mineralization, as an essential step of fast implant integration with vivid tissues.

Development of Titanium Implants with a Rough Calcium Phosphate Surface to Control the Morphofunctional State of Stem Cells

2021 ◽  
Vol 887 ◽  
pp. 40-45
Author(s):  
K.A. Yurova ◽  
Igor A. Khlusov ◽  
L.S. Litvinova
Keyword(s):  
Stem Cells ◽  
Calcium Phosphate ◽  
Bone Cells ◽  
Titanium Implants ◽  
New Class ◽  
Physicochemical Features ◽  
Mineral Substance ◽  
Titanium Substrates

Experimental titanium implants with a rough calcium phosphate (CР) coating with bio-inspired properties have been developed. The role of roughness (in the range Ra = 2.4 - 4.6 μm) of the surface CP simulating the physicochemical features of the mineral substance of regenerating bone tissue to change the growth and morphofunctional activity of mesenchymal stem cells (MSCs) was evaluated. Titanium substrates with a microrelief CP coating bearing artificial niches for stem cells was shown in vitro to have an epigenomic effect on MSCs, that contributes to their differentiation and maturation in bone cells. The results show the promise of developing and introducing a new class of medical devices with bio-inspired surfaces into clinical practice for traumatology and orthopedics.

Calcium Phosphate Coating on Blast-Treated Titanium Implants by RF Magnetron Sputtering

2009 ◽  
Vol 631-632 ◽  
pp. 211-216 ◽  
Author(s):  
Kyosuke Ueda ◽  
Takayuki Narushima ◽  
Takashi Goto ◽  
T. Katsube ◽  
Hironobu Nakagawa ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Magnetron Sputtering ◽  
Bonding Strength ◽  
Rf Magnetron Sputtering ◽  
Titanium Implants ◽  
Calcium Phosphate Coating ◽  
Phosphate Coating ◽  
Coating Films

Calcium phosphate coating films were fabricated on Ti-6Al-4V plates and screw-type implants with a blast-treated surface using radiofrequency (RF) magnetron sputtering and were evaluated in vitro and in vivo. Amorphous calcium phosphate (ACP) and oxyapatite (OAp) films obtained in this study could cover the blast-treated substrate very efficiently, maintaining the surface roughness. For the in vitro evaluations of the calcium phosphate coating films, bonding strength and alkaline phosphatase (ALP) activity were examined. The bonding strength of the coating films to a blast-treated substrate exceeded 60 MPa, independent of film phases except for the film after post-heat-treatment in silica ampoule. When compared with an uncoated substrate, the increase in the ALP activity of osteoblastic SaOS-2 cells on a calcium phosphate coated substrate was confirmed by a cell culture test. The removal torque of screw-type Ti-6Al-4V implants with a blast-treated surface from the femur of Japanese white rabbit increased with the duration of implantation and it was statistically improved by coating an ACP film 2 weeks after implantation. The in vitro and in vivo studies suggested that the application of the sputtered ACP film as a coating on titanium implants was effective in improving their biocompatibility with bones.

Electrochemical Deposition of Fluoridated Calcium Phosphate Thin Film on Titanium Substrates

2008 ◽  
Vol 47-50 ◽  
pp. 1387-1390 ◽  
Author(s):  
Xiang Ge ◽  
Fu Zeng Ren ◽  
Yang Leng
Keyword(s):  
Thin Film ◽  
Calcium Phosphate ◽  
Dental Implants ◽  
Titanium Substrate ◽  
Titanium Implants ◽  
Connective Tissues ◽  
Percutaneous Device ◽  
Fluorine Ions ◽  
Film Synthesis ◽  
Titanium Substrates

Percutaneous type of orthopedic and dental implants requires not only a good adhesion with bone, but also the ability to form good attachment and seal with connective tissues and skins. Currently, the skin-seal of such implants still remains as a problem to be resolved. Electrochemical processing was used to modify the surface of titanium implants in order to improve the ability of anti-bacteria infection and skin seal around the implants by synthesizing a fluoridated calcium phosphate thin film on titanium substrate. The surface of titanium was cathodically treated in an electrochemical cell. A thin film of about 80 nm thickness was deposited on the titanium surface by controlling the treatment parameters. The dense and gel-like film was composed of calcium phosphate and fluorine ions. Fluorine ion has the anti-bacteria property and could help to improve the skin seal around the percutaneous device. The electrochemical method of fluoridated calcium phosphate thin film synthesis will provide an alternative method for surface treatment of orthopedic and dental implants.

Bioactive and Antibacterial Ag-AgCl-TiO2 Coating on Titanium Implants

2016 ◽  
Vol 852 ◽  
pp. 1213-1219 ◽  
Author(s):  
Tian Tian ◽  
Liu Hui ◽  
Gu Ming Jun ◽  
Jin Ying
Keyword(s):  
Bacterial Adhesion ◽  
Antibacterial Property ◽  
Titanium Implants ◽  
Material Surface ◽  
X Ray Diffraction ◽  
E Coli ◽  
Implant Material ◽  
Titanium Substrates ◽  
Soaking Test

In this work, Ag-AgCl-TiO2 coating was fabricated on titanium substrates to obtain an implant material having excellent antibacterial property and bioactivity. The coating was investigated by scanning electron microscopy and X-ray diffraction. The bioactivity of coatings was examined by simulated body fluid soaking test. To verify the susceptibility of implant material surface to bacterial adhesion, S. aureus (Sau), E. coli (Eco), K. pneumoniae (Kpn), P. Aeruginosa (Pae), four types of major pathogen were chosen for in vitro antibacterial analyses. The results showed that Ag-AgCl-TiO2 coating had excellent antibacterial property and bioactivity.

A comparative study of calcium phosphate formation on bioceramics in vitro and in vivo

Biomaterials ◽  
2005 ◽  
Vol 26 (33) ◽  
pp. 6477-6486 ◽  
Author(s):  
Renlong Xin ◽  
Yang Leng ◽  
Jiyong Chen ◽  
Qiyi Zhang
Keyword(s):  
Calcium Phosphate ◽  
Comparative Study

scholarly journals Composite Drug Delivery System Based on Amorphous Calcium Phosphate–Chitosan: An Efficient Antimicrobial Platform for Extended Release of Tetracycline

Pharmaceutics ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1659
Author(s):  
Anita Ioana Visan ◽  
Carmen Ristoscu ◽  
Gianina Popescu-Pelin ◽  
Mihai Sopronyi ◽  
Consuela Elena Matei ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Extended Release ◽  
Composite Coatings ◽  
Burst Release ◽  
Large Area ◽  
Drug Release Profile ◽  
Composite Mixture ◽  
Drug Incorporation

One major warning emerging during the first worldwide combat against healthcare-associated infections concerns the key role of the surface in the storage and transfer of the virus. Our study is based on the laser coating of surfaces with an inorganic/organic composite mixture of amorphous calcium phosphate–chitosan–tetracycline that is able to fight against infectious agents, but also capable of preserving its activity for a prolonged time, up to several days. The extended release in simulated fluids of the composite mixture containing the drug (tetracycline) was demonstrated by mass loss and UV–VIS investigations. The drug release profile from our composite coatings proceeds via two stages: an initial burst release (during the first hours), followed by a slower evolution active for the next 72 h, and probably more. Optimized coatings strongly inhibit the growth of tested bacteria (Enterococcus faecalis and Escherichia coli), while the drug incorporation has no impact on the in vitro composite’s cytotoxicity, the coatings proving an excellent biocompatibility sustaining the normal development of MG63 bone-like cells. One may, therefore, consider that the proposed coatings’ composition can open the prospective of a new generation of antimicrobial coatings for implants, but also for nosocomial and other large area contamination prevention.

In Vitro Evaluation of RF Magnetron-Sputtered Calcium Phosphate Films on Titanium

2007 ◽  
Vol 352 ◽  
pp. 305-309
Author(s):  
Kyosuke Ueda ◽  
Takayuki Narushima ◽  
Tomoyuki Katsube ◽  
Hiroshi Kawamura ◽  
Takashi Goto
Keyword(s):  
Calcium Phosphate ◽  
Calcium Ions ◽  
Rf Magnetron Sputtering ◽  
Calcium Phosphate Coating ◽  
Apatite Formation ◽  
Coating Films ◽  
Before And After ◽  
Phosphate Buffered Saline ◽  
Titanium Substrates

Calcium phosphate coating films were fabricated on mirror-polished or blast-treated titanium substrates using radio-frequency (RF) magnetron sputtering and they were evaluated in vitro. Immersion tests for the films were conducted using phosphate-buffered saline (PBS(-)), and apatite formation and the elution of calcium ions from the films were investigated. The bonding strengths between the calcium phosphate films and titanium substrates before and after the immersion tests were evaluated. After the immersion tests, a decrease in the bonding strength was observed for the coating films on the mirror-polished titanium substrates, while that for the blast-treated titanium substrates was almost the same as that before the immersion tests.

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