scholarly journals Structural Influence on the Post-Clustering Stability of DNA/AgNCs Fluorescence

Nanomaterials ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 667 ◽  
Author(s):  
Riddhi Nagda ◽  
Pratik Shah ◽  
Chang Seop Lee ◽  
Sooyeon Park ◽  
Seong Wook Yang
Keyword(s):  
Atmospheric Oxygen ◽  
Extended Period ◽  
Silver Nanoclusters ◽  
Enzymatic Assays ◽  
Practical Applications ◽  
Long Term Stability ◽  
Orange Fluorescence ◽  
Dna Secondary Structures ◽  
Post Synthesis ◽  
Dna Template

DNA-encapsulated Silver Nanoclusters (DNA/AgNCs) based sensors have gained increasing attention in past years due to their diverse applications in bioimaging, biosensing, and enzymatic assays. Given the potential of DNA/AgNCs for practical applications, the systematic studies of the fluorescent stability over an extended period is necessary. However, the correlation between nucleic acid properties and the long-term stability of DNA/AgNCs is less known. With locking-to-unlocking sensors, in which the secondary structure of DNA template is standardized, we investigated the correlation between the DNA structure and the fluorescence stability of AgNCs. Post-synthesis of DNA/AgNCs, the fluorescence, and structures of templates were monitored over three weeks. By combining the fluorescence spectroscopy with the in-gel fluorescent assay, we found that AgNCs encapsulated by dimer-structured DNA/AgNCs templates were more stable than those of hairpin-structured DNA/AgNCs templates. While the orange fluorescence from the dimer templates increased over three weeks, the red fluorescence from the hairpin templates was diminished by >80% within two days at room temperature. Further tests revealed that hairpin-encapsulated red-emissive AgNCs is more sensitive to oxidation by atmospheric oxygen compared to dimer encapsulated orange AgNCs. Our observations may provide an important clue in encapsulating photophysically more stable AgNCs by tuning the DNA secondary structures. The proposed strategy here can be essential for pragmatic applications of DNA/AgNCs templates.

scholarly journals Corrigendum to “Formation of Silver Nanoclusters from a DNA Template Containing Ag(I)-Mediated Base Pairs”

2018 ◽  
Vol 2018 ◽  
pp. 1-1
Author(s):  
J. Christian Léon ◽  
Linda Stegemann ◽  
Martin Peterlechner ◽  
Stefanie Litau ◽  
Gerhard Wilde ◽  
...  
Keyword(s):  
Silver Nanoclusters ◽  
Base Pairs ◽  
Dna Template

DNA template-regulated intergrowth of a fluorescent silver nanocluster emitter pair

RSC Advances ◽  
2015 ◽  
Vol 5 (119) ◽  
pp. 98467-98471 ◽  
Author(s):  
Jin-Liang Ma ◽  
Bin-Cheng Yin ◽  
Bang-Ce Ye
Keyword(s):  
Silver Nanoclusters ◽  
Silver Nanocluster ◽  
Dna Template

We have developed a novel type of intensely fluorescent DNA-templated silver nanoclusters (DNA/AgNCs), which is in the form of the intergrowth of a Ag emitter pair.

Progress on the electrolytes for dye-sensitized solar cells

2008 ◽  
Vol 80 (11) ◽  
pp. 2241-2258 ◽  
Author(s):  
Jihuai Wu ◽  
Zhang Lan ◽  
Sanchun Hao ◽  
Pingjiang Li ◽  
Jianming Lin ◽  
...  
Keyword(s):  
Solar Cells ◽  
Solid State ◽  
Low Cost ◽  
Electrical Power ◽  
Dye Sensitized Solar Cells ◽  
Practical Applications ◽  
Long Term Stability ◽  
Dye Sensitized ◽  
Solid State Electrolytes ◽  
Sensitized Solar Cells

Dye-sensitized solar cells (DSSCs) have aroused intense interest over the past decade owing to their low cost and simple preparation procedures. Much effort has been devoted to the study of electrolytes that enable light-to-electrical power conversion for DSSC applications. This review focuses on recent progress in the field of liquid, solid-state, and quasi-solid-state electrolytes for DSSCs. It is believed that quasi-solid-state electrolytes, especially those utilizing thermosetting gels, are particularly applicable for fabricating high photoelectric performance and long-term stability of DSSCs in practical applications.

scholarly journals Replication of G Quadruplex DNA

Genes ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 95 ◽  
Author(s):  
Leticia Koch Lerner ◽  
Julian E. Sale
Keyword(s):  
Dna Replication ◽  
Dna Sequences ◽  
Secondary Structures ◽  
Dna Unwinding ◽  
Chromosomal Dna ◽  
Quadruplex Dna ◽  
Epigenetic Instability ◽  
G Quadruplex ◽  
Dna Secondary Structures ◽  
Dna Template

A cursory look at any textbook image of DNA replication might suggest that the complex machine that is the replisome runs smoothly along the chromosomal DNA. However, many DNA sequences can adopt non-B form secondary structures and these have the potential to impede progression of the replisome. A picture is emerging in which the maintenance of processive DNA replication requires the action of a significant number of additional proteins beyond the core replisome to resolve secondary structures in the DNA template. By ensuring that DNA synthesis remains closely coupled to DNA unwinding by the replicative helicase, these factors prevent impediments to the replisome from causing genetic and epigenetic instability. This review considers the circumstances in which DNA forms secondary structures, the potential responses of the eukaryotic replisome to these impediments in the light of recent advances in our understanding of its structure and operation and the mechanisms cells deploy to remove secondary structure from the DNA. To illustrate the principles involved, we focus on one of the best understood DNA secondary structures, G quadruplexes (G4s), and on the helicases that promote their resolution.

scholarly journals Recent Progress on Stability and Thermo-Physical Properties of Mono and Hybrid towards Green Nanofluids

Micromachines ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 176
Author(s):  
S.N.M. Zainon ◽  
W.H. Azmi
Keyword(s):  
Physical Properties ◽  
Visual Observation ◽  
Particle Volume ◽  
Factors Affecting ◽  
Practical Applications ◽  
Long Term Stability ◽  
Thermal Fluids ◽  
Vis Spectroscopy ◽  
The Stability ◽  
Thermo Physical Properties

Many studies have shown the remarkable enhancement of thermo-physical properties with the addition of a small quantity of nanoparticles into conventional fluids. However, the long-term stability of the nanofluids, which plays a significant role in enhancing these properties, is hard to achieve, thus limiting the performance of the heat transfer fluids in practical applications. The present paper attempts to highlight various approaches used by researchers in improving and evaluating the stability of thermal fluids and thoroughly explores various factors that contribute to the enhancement of the thermo-physical properties of mono, hybrid, and green nanofluids. There are various methods to maintain the stability of nanofluids, but this paper particularly focuses on the sonication process, pH modification, and the use of surfactant. In addition, the common techniques to evaluate the stability of nanofluids are undertaken by using visual observation, TEM, FESEM, XRD, zeta potential analysis, and UV-Vis spectroscopy. Prior investigations revealed that the type of nanoparticle, particle volume concentration, size and shape of particles, temperature, and base fluids highly influence the thermo-physical properties of nanofluids. In conclusion, this paper summarized the findings and strategies to enhance the stability and factors affecting the thermal conductivity and dynamic viscosity of mono and hybrid of nanofluids towards green nanofluids.

scholarly journals THE ASSESSMENT OF BANKRUPTCY RISKS OF CONSTRUCTION COMPANIES

2017 ◽  
pp. 218-232
Author(s):  
Anna Dudkowska ◽  
Tadeusz Waściński
Keyword(s):  
Early Warning ◽  
Extended Period ◽  
Early Warning Systems ◽  
Financial Data ◽  
Warning Systems ◽  
Practical Applications ◽  
Construction Companies ◽  
Financial Difficulties ◽  
A Company ◽  
Day By Day

Companies don’t fail on a day by Day basis. Usually the decline of a economic unit is accompanied by decreasing economic and financial situation over an extended period of time, which is usually five years, according to the literature. Therefore, companies have time to react and undertake appropriate countermeasures, but they need to be aware of risk’s existence and it’s scale. In this article symptoms indicating at declining standing of a company, early warning systems’ essence and their classification are shown. Sample systems identifying economical and financial difficulties of companies were described and using chosen early warning systems the assessment of risk of the bankruptcy of public limited companies Budimex, Erbud and AWbud was performed, based on the financial data of the examined companies from years 2012-2015. Aim of the research paper is to bring to reader’s attention the enormous significance of early warning systems and their practical applications.

scholarly journals Fluorescent AgNCs Formed on Bifunctional DNA Template for Potassium Ion Detection

Chemosensors ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 349
Author(s):  
Patrycja Filipczuk ◽  
Angelika Świtalska ◽  
Joanna Kosman ◽  
Grzegorz Nowaczyk ◽  
Anna Dembska
Keyword(s):  
Structural Changes ◽  
Green Emission ◽  
Potassium Transport ◽  
Silver Nanoclusters ◽  
Fluorescence Signal ◽  
Red Emission ◽  
Binding Preference ◽  
G Quadruplex ◽  
Na Ions ◽  
Dna Template

In this study, we examined properties of silver nanoclusters, which are AgNCs stabilized by DNA oligonucleotide scaffold containing G-quadruplex-forming sequences: human telomeric (Tel22) or thrombin-binding aptamer (TBA). Thus, we obtained two fluorescent probes abbreviated as Tel22C12-AgNCs and TBAC12-AgNCs, which were characterized using absorption, circular dichroism and fluorescence spectroscopy. Both probes emit green and red fluorescence. The presence of silver nanoclusters did not destabilize the formed G-quadruplexes. The structural changes of probes upon binding K+ or Na+ ions cause quenching in their red emission. Green emission was slightly quenched only in the case of Tel22C12-AgNCs; on the contrary, for TBAC12-AgNC’s green emission, we observed an increasing fluorescence signal. Moreover, the Tel22C12-AgNCs system shows not only a higher binding preference for K+ over Na+, but it was able to monitor small changes in K+ concentrations in the buffer mimicking extracellular conditions (high content of Na+ ions). These results suggest that Tel22C12-AgNCs exhibit the potential to monitor transmembrane potassium transport.

scholarly journals Functionalized-AgNPs for Long-Term Stability and Its Applicability in the Detection of Manganese Ions

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Van-Tuan Hoang ◽  
Mai Mai ◽  
Le Thi Tam ◽  
Ngoc Phan Vu ◽  
Nguyen Tien Khi ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Aqueous Dispersion ◽  
Tween 80 ◽  
Strong Interactions ◽  
Electrostatic Repulsion ◽  
Practical Applications ◽  
Term Stability ◽  
Long Term Stability ◽  
Long Time

In this study, silver nanoparticles (AgNPs) were functionalized by various molecules, including sodium borohydride (NaBH4), polyhexamethylene biguanide hydrochloride (PHMB), and Tween 80 to investigate the long-term stabilization of AgNPs in an aqueous dispersion. PHMB-functionalized silver nanoparticles (AgNPs/PHMB) exhibited better stability than others and could be stored at ambient temperature for at least 180 days. In addition to creating stabilization based on the electrostatic repulsion, the use of PHMB helped to increase the degree of stability of the colloidal AgNPs for a long time owing to strong interactions between Ag atoms on AgNPs with nitrogen (N) positions in PHMB molecules. The formed bond led to improving maintenance ability of the electrostatic repulsion layer among independent nanoparticles. The applicability of the as-prepared AgNPs/PHMB was also examined for Mn2+ detection via a colorimetric approach. The calibration curve was found to be linear over the range of 0–100 mM with a correlation coefficient of 0.97. The amine groups of PHMB brought out a cooperative effect to form of ion-templated chelation with Mn2+, which caused the aggregation of AgNPs/PHMB. This suggested that the AgNPs/PHMB could be used as a potential probe in the detection of Mn2+ ions. More importantly, the long-term stability of AgNPs/PHMB paved a great promising path to provide many further solutions for the producer in practical applications.

Electronic Failure Analysis of Plate-Capacitors Bonding with Conductive Adhesives

2014 ◽  
Vol 788 ◽  
pp. 569-572
Author(s):  
Zhi Ming Song ◽  
Hong Wei Li ◽  
Bin Wang ◽  
Qian Tao Cao ◽  
Ying Lu Hu ◽  
...  
Keyword(s):  
Failure Analysis ◽  
Direct Current ◽  
Silver Particles ◽  
Conductive Adhesives ◽  
Practical Applications ◽  
Long Term Stability ◽  
Microelectronic Devices ◽  
Humid Environment ◽  
Dc Electric Field

The long-term stability of microelectronic devices is crucial for their practical applications. However, silver particles in the conductive adhesive can migrate easily under certain conditions, resulting in failure of the devices. In this investigation, the migration of silver particles has been studied by using the devices of Plate-capacitors in the cavity that are bonded with conductive adhesives. The experimental results show that the migration of silver particles occurred in the condition of humid environment with an applied direct current (DC) electric field. Also, the migration of silver particles under a variety of humidity was analyzed, and the reasons causing the failure of the devices were demonstrated.

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