scholarly journals Sacrificial Core-Based Electrospinning: A Facile and Versatile Approach to Fabricate Devices for Potential Cell and Tissue Encapsulation Applications

Nanomaterials ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 863 ◽  
Author(s):  
Naresh Kasoju ◽  
Julian George ◽  
Hua Ye ◽  
Zhanfeng Cui
Keyword(s):  
Cell Encapsulation ◽  
Cell Types ◽  
Hollow Core ◽  
Protein Diffusion ◽  
Polyamide 66 ◽  
Encapsulated Cells ◽  
Path Control ◽  
Model Cell ◽  
3D Printed ◽  
Dta Studies

Electrospinning uses an electric field to produce fine fibers of nano and micron scale diameters from polymer solutions. Despite innovation in jet initiation, jet path control and fiber collection, it is common to only fabricate planar and tubular-shaped electrospun products. For applications that encapsulate cells and tissues inside a porous container, it is useful to develop biocompatible hollow core-containing devices. To this end, by introducing a 3D-printed framework containing a sodium chloride pellet (sacrificial core) as the collector and through post-electrospinning dissolution of the sacrificial core, we demonstrate that hollow core containing polyamide 66 (nylon 66) devices can be easily fabricated for use as cell encapsulation systems. ATR-FTIR and TG/DTA studies were used to verify that the bulk properties of the electrospun device were not altered by contact with the salt pellet during fiber collection. Protein diffusion investigations demonstrated that the capsule allowed free diffusion of model biomolecules (insulin, albumin and Ig G). Cell encapsulation studies with model cell types (fibroblasts and lymphocytes) revealed that the capsule supports the viability of encapsulated cells inside the capsule whilst compartmentalizing immune cells outside of the capsule. Taken together, the use of a salt pellet as a sacrificial core within a 3D printed framework to support fiber collection, as well as the ability to easily remove this core using aqueous dissolution, results in a biocompatible device that can be tailored for use in cell and tissue encapsulation applications.

Proliferation and Interactions of Several Cell Types Encapsulated Within Dense (Non-Porous) Protein-Permeable Polyurethane Membranes

1993 ◽  
Vol 331 ◽  
Author(s):  
Albert Y. Wang ◽  
Robert S. Ward ◽  
Kathleen A. White ◽  
Robert W. Kuhn ◽  
Julie E. Taylor ◽  
...  
Keyword(s):  
Fibrous Tissue ◽  
Cell Encapsulation ◽  
Cell Types ◽  
Artificial Organs ◽  
Encapsulated Cells ◽  
Polyurethane Membranes ◽  
Dependent Cell ◽  
And Function

AbstractProtein-permeable dense (non-porous) urethane membranes have been evaluated for in vitro cell culture, and in vivo cell encapsulation. Polyurethane membranes were designed to exhibit permeability to proteins, gases, and nutrients without the existence of pores. The membranes are non-cytotoxic, angiogenic, and permeable to gases, nutrients, secretagogues and cell products via purely concentration-driven transport. Non-anchorage and anchorage dependent cells were grown encapsulated within the membrane and with the membrane as a growth substrate. Several non-anchorage dependent cell types proliferated within the membrane both in-vitro and in-vivo. Anchorage-dependent cells were grown on the membranes as a substrate. Encapsulated cells have been maintained in culture for up to six months with nutrients supplied only by the external media. Immuno-isolation has been demonstrated with cells implanted into murine hosts. Explants of membrane encapsulated cells exhibited a high degree of vascularization, with little or no fibrous tissue. The ability to support cell growth and function, and the ability to protect xenogenic cells from immunologic rejection suggest that the membranes would be useful in the construction of hybrid artificial organs, devices for cell transplantation, and substrates for cell and tissue culture.

scholarly journals 2P289 Optimization of the cell encapsulation in the water in oil droplet using 3D printed object(26. Measurements,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

2014 ◽  
Vol 54 (supplement1-2) ◽  
pp. S243
Author(s):  
Mathias Girault ◽  
Akihiro Hattori ◽  
Hyonchol Kim ◽  
Kenji Matsuura ◽  
Masao Odaka ◽  
...  
Keyword(s):  
Annual Meeting ◽  
Cell Encapsulation ◽  
Oil Droplet ◽  
Biophysical Society ◽  
3D Printed

Structure, Process, and Material Influences for 3D Printed Lattices Designed With Mixed Unit Cells

2020 ◽  
Author(s):  
Gabriel Briguiet ◽  
Paul F. Egan
Keyword(s):  
Elastic Modulus ◽  
Mechanical Testing ◽  
Mechanical Response ◽  
Cell Types ◽  
Unit Cell ◽  
Ultraviolet Curing ◽  
Mechanical Responses ◽  
Lattice Design ◽  
Unit Cells ◽  
3D Printed

Abstract Emerging 3D printing technologies are enabling the design and fabrication of novel architected structures with advantageous mechanical responses. Designing complex structures, such as lattices, with a targeted response is challenging because build materials, fabrication process, and topological design have unique influences on the structure’s mechanical response. Changing any factor may have unanticipated consequences, even for simpler lattice structures. Here, we conduct mechanical compression experiments to investigate varied lattice design, fabrication, and material combinations using stereolithography printing with a biocompatible polymer. Mechanical testing demonstrates that a higher ultraviolet curing time increases elastic modulus. Material testing demonstrated that anisotropy does not strongly influence lattice mechanics. Designs were altered by comparing homogenous lattices of single unit cell types and heterogeneous lattices that combine two types of unit cells. Unit cells for heterogeneous structures include a Cube design for a high elastic modulus and Cross design for improved shear response. Mechanical testing of three heterogeneous layouts demonstrated how unit cell organization influences mechanical outcomes, therefore enabling the tuning of an elastic modulus that surpasses the law of averages designed for application-dependent mechanical needs. These findings provide a foundation for linking design, process, and material for engineering 3D printed structures with preferred properties, while also facilitating new directions in design automation and optimization.

scholarly journals Complementary techniques to analyse pericellular matrix formation by human MSC within hyaluronic acid hydrogels

2020 ◽  
Vol 1 (8) ◽  
pp. 2888-2896
Author(s):  
Christoph Salzlechner ◽  
Anders Runge Walther ◽  
Sophie Schell ◽  
Nicholas Groth Merrild ◽  
Tabasom Haghighi ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Amino Acid ◽  
Cell Encapsulation ◽  
Pericellular Matrix ◽  
Encapsulated Cells ◽  
The Matrix ◽  
Canonical Amino Acid ◽  
Confocal Raman ◽  
Complementary Techniques ◽  
Raman Spectral

Hydrogels are used widely for cell encapsulation to mimic the native ECM. Here, we characterise and visualise the matrix secreted by encapsulated cells by combining fluorescent non-canonical amino acid tagging with confocal Raman spectral imaging.

scholarly journals Mid-IR Hollow-core microstructured fiber drawn from a 3D printed PETG preform

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Wanvisa Talataisong ◽  
Rand Ismaeel ◽  
Thiago H. R. Marques ◽  
Seyedmohammad Abokhamis Mousavi ◽  
Martynas Beresna ◽  
...  
Keyword(s):  
Hollow Core ◽  
Microstructured Fiber ◽  
3D Printed

Mechanical characterization and numerical simulation of a subcutaneous implantable 3D printed cell encapsulation system

2018 ◽  
Vol 82 ◽  
pp. 133-144 ◽  
Author(s):  
Federica Adamo ◽  
Marco Farina ◽  
Usha R. Thekkedath ◽  
Alessandro Grattoni ◽  
Raffaella Sesana
Keyword(s):  
Numerical Simulation ◽  
Mechanical Characterization ◽  
Cell Encapsulation ◽  
3D Printed

scholarly journals 3D Printed Hollow-Core Terahertz Fibers

Fibers ◽  
2018 ◽  
Vol 6 (3) ◽  
pp. 43 ◽  
Author(s):  
Alice Cruz ◽  
Cristiano Cordeiro ◽  
Marcos Franco
Keyword(s):  
Hollow Core ◽  
3D Printed

scholarly journals 3D printed lung on a chip device with a stretchable nanofibrous membrane for modeling ventilator induced lung injury

2021 ◽  
Author(s):  
Sinem Tas ◽  
Emil Rehnberg ◽  
Deniz A. Bölükbaş ◽  
Jason P. Beech ◽  
Liora Nasi Kazado ◽  
...  
Keyword(s):  
Cell Death ◽  
Epithelial Cells ◽  
Lung Injury ◽  
Mechanical Stretch ◽  
Cell Types ◽  
Lung Epithelial Cells ◽  
Nanofibrous Membrane ◽  
Ventilator Induced Lung Injury ◽  
Lung Epithelial ◽  
3D Printed

Mechanical ventilation is often required in patients with pulmonary disease to maintain adequate gas exchange. Despite improved knowledge regarding the risks of over ventilating the lung, ventilator induced lung injury (VILI) remains a major clinical problem due to inhomogeneities within the diseased lung itself as well as the need to increase pressure or volume of oxygen to the lung as a life-saving measure. VILI is characterized by increased physical forces exerted within the lung, which results in cell death, inflammation and long-term fibrotic remodeling. Animal models can be used to study VILI, but it is challenging to distinguish the contributions of individual cell types in such a setup. In vitro models, which allow for controlled stretching of specific lung cell types have emerged as a potential option, but these models and the membranes used in them are unable to recapitulate some key features of the lung such as the 3D nanofibrous structure of the alveolar basement membrane while also allowing for cells to be cultured at an air liquid interface (ALI) and undergo increased mechanical stretch that mimics VILI. Here we develop a lung on a chip device with a nanofibrous synthetic membrane to provide ALI conditions and controllable stretching, including injurious stretching mimicking VILI. The lung on a chip device consists of a thin (i.e. ~20 μm) stretchable poly(caprolactone) (PCL) nanofibrous membrane placed between two channels fabricated in polydimethylsiloxane (PDMS) using 3D printed molds. We demonstrate that this lung on a chip device can be used to induce mechanotrauma in lung epithelial cells due to cyclic pathophysiologic stretch (~25%) that mimics clinical VILI. Pathophysiologic stretch induces cell injury and subsequently cell death, which results in loss of the epithelial monolayer, a feature mimicking the early stages of VILI. We also validate the potential of our lung on a chip device to be used to explore cellular pathways known to be altered with mechanical stretch and show that pathophysiologic stretch of lung epithelial cells causes nuclear translocation of the mechanotransducers YAP/TAZ. In conclusion, we show that a breathable lung on a chip device with a nanofibrous membrane can be easily fabricated using 3D printing of the lung on a chip molds and that this model can be used to explore pathomechanisms in mechanically induced lung injury.

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