scholarly journals Development of a Nanostructured Platform for Identifying HER2-Heterogeneity of Breast Cancer Cells by Surface-Enhanced Raman Scattering

Nanomaterials ◽  
2018 ◽  
Vol 8 (7) ◽  
pp. 549 ◽  
Author(s):  
Alexandro Téllez-Plancarte ◽  
Emmanuel Haro-Poniatowski ◽  
Michel Picquart ◽  
José Morales-Méndez ◽  
Carlos Lara-Cruz ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Raman Scattering ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Surface Enhanced Raman Scattering ◽  
Laser Scanning Microscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Mcf 7

Biosensor technology has great potential for the detection of cancer through tumor-associated molecular biomarkers. In this work, we describe the immobilization of the recombinant humanized anti-HER2 monoclonal antibody (trastuzumab) on a silver nanostructured plate made by pulsed laser deposition (PLD), over a thin film of Au(111). Immobilization was performed via 4-mercapto benzoic acid self-assembled monolayers (4-MBA SAMs) that were activated with coupling reagents. A combination of immunofluorescence images and z-stack analysis by confocal laser scanning microscopy (CLSM) allowed us to detect HER2 presence and distribution in the cell membranes. Four different HER2-expressing breast cancer cell lines (SKBR3 +++, MCF-7 +/−, T47D +/−, MDA-MB-231 −) were incubated during 24 h on functionalized silver nanostructured plates (FSNP) and also on Au(111) thin films. The cells were fixed by means of an ethanol dehydration train, then characterized by atomic force microscopy (AFM) and surface-enhanced Raman scattering (SERS). SERS results showed the same tendency as CLSM findings (SKBR3 > MCF-7 > T47D > MDA-MB-231), especially when the Raman peak associated with phenylalanine amino acid (1002 cm−1) was monitored. Given the high selectivity and high sensitivity of SERS with a functionalized silver nanostructured plate (FSNP), we propose this method for identifying the presence of HER2 and consequently, of breast cancer cells.

Surface-Enhanced Raman Scattering Optophysiology Nanofibers for the Detection of Heavy Metals in Single Breast Cancer Cells

ACS Sensors ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 1649-1662
Author(s):  
Xingjuan Zhao ◽  
Shirley Campbell ◽  
Patrick Z. El-Khoury ◽  
Yuechen Jia ◽  
Gregory Q. Wallace ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Heavy Metals ◽  
Raman Scattering ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Surface Enhanced Raman Scattering ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Distinguishing breast cancer cells using surface-enhanced Raman scattering

2011 ◽  
Vol 402 (3) ◽  
pp. 1093-1100 ◽  
Author(s):  
Jing Yang ◽  
Zhuyuan Wang ◽  
Shenfei Zong ◽  
Chunyuan Song ◽  
Ruohu Zhang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Raman Scattering ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Surface Enhanced Raman Scattering ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

scholarly journals Controlled release of targeted anti-leukemia drugs azacitidine and decitabine monitored using surface-enhanced Raman scattering (SERS) spectroscopy

2017 ◽  
Vol 6 (4) ◽  
pp. 125-132 ◽  
Author(s):  
Madison Smith ◽  
Maria Hepel
Keyword(s):  
Drug Delivery ◽  
Folic Acid ◽  
Controlled Release ◽  
Drug Release ◽  
Raman Scattering ◽  
Cancer Cells ◽  
Surface Enhanced Raman Scattering ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

A new targeted drug delivery system with controlled release of anti-cancer drugs, azacitidine and decitabine, was investigated to enhance the efficacy of cancer treatment and reduce the effects of high drug toxicity to healthy tissues. The proposed drug nanocarriers are based on gold nanoparticles (AuNPs) modified with mercaptobenzoic acid (MBA) linker to enable the immobilization of azacitidine (AZA) and decitabine (DAC) on AuNPs in the form of AuNP@MBA/AZA,DAC entities. The cancer cell recognition was accomplished by covalently binding folic acid (FA) ligands to para-aminothiophenol (PATP) in the mixed SAM shell on gold nanoparticle nanocarriers, AuNP@MBA,PATP. The FA ligand was used due to the strong expression of folic acid receptors (FR) in the membrane of cancer cells. This enables the functionalized carriers to target only cancer cells owing to the efficient FA-FR binding property. The amide bonds between the linkers and azacitidine/decitabine are pH sensitive and undergo acid hydrolysis in a low pH environment of the cytosol in cancer cells. Using the solutions of different pH, the release of azacitidine/decitabine was monitored by surface-enhanced Raman scattering spectroscopy (SERS) measurements of the MBA Raman modes at 1586 cm-1 and 1074 cm-1 . At pH 7.4, the release of the drug was found to be negligible, while at pH 4.0 and 5.5 a continuous drug release was observed over 3 hours. The utilization of SERS monitoring for the drug release was based on the strong Raman signals which are generated by the MBA linker when it is bound to a plasmonic AuNP. During the immobilization of azacitidine/decitabine on AuNP carriers, the SERS signals are strongly reduced due to the shielding by drug molecules but they increase sharply upon the drug release confirming the amide bond breakage and successful drug delivery.

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