scholarly journals Biocompatible Gold Nanoparticles Ameliorate Retinoic Acid-Induced Cell Death and Induce Differentiation in F9 Teratocarcinoma Stem Cells

Nanomaterials ◽  
2018 ◽  
Vol 8 (6) ◽  
pp. 396 ◽  
Author(s):  
Sangiliyandi Gurunathan ◽  
Jin-Hoi Kim
Keyword(s):  
Stem Cells ◽  
Gold Nanoparticles ◽  
Cell Death ◽  
Retinoic Acid ◽  
F9 Teratocarcinoma

scholarly journals Retinoic Acid Receptor Isotype Specificity in F9 Teratocarcinoma Stem Cells Results from the Differential Recruitment of Coregulators to Retinoic Acid Response Elements

2007 ◽  
Vol 282 (46) ◽  
pp. 33421-33434 ◽  
Author(s):  
Robert F. Gillespie ◽  
Lorraine J. Gudas
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Target Genes ◽  
Retinoic Acid Receptor ◽  
Polycomb Group ◽  
Polycomb Group Protein ◽  
Response Elements ◽  
Acid Receptor ◽  
Group Protein ◽  
F9 Teratocarcinoma

The retinoic acid receptor (RAR) α, β2, and γ isotypes each regulate specific subsets of target genes in F9 teratocarcinoma stem cells. We used chromatin immunoprecipitation assays to monitor the association of RARγ, retinoic X receptor (RXR) α, and coregulators with the RARβ2, Hoxa1, and Cyp26A1 retinoic acid response elements (RAREs) in F9 wild type and RARα, -β2, and -γ null cells. Additionally we quantitatively monitored expression of the corresponding mRNAs. We demonstrated that the association of RARγ and/or RXRα with a RARE was not sufficient for retinoic acid (RA)-mediated transcription of the corresponding target gene. However, the ability of RARγ and/or RXRα to recruit pCIP (AIB1/ACTR/RAC-3/TRAM-1/SRC-3) and p300 to a RARE did correlate with RA-associated transcription of target mRNAs. Therefore, the specific functions of the RAR isotypes do not manifest at the level of their DNA binding but rather from a differential ability to recruit specific components of the transcriptional machinery. We also demonstrated that RA-mediated displacement of the polycomb group protein SUZ12 from a RARE was inhibited in the absence of RARγ. Thus, transcriptional components of the RAR signaling pathway are specifically required for displacement of SUZ12 from RAREs during RA-mediated differentiation of F9 cells.

An octamer motif contributes to the expression of the retinoic acid-regulated zinc finger gene Rex-1 (Zfp-42) in F9 teratocarcinoma cells

1993 ◽  
Vol 13 (5) ◽  
pp. 2919-2928
Author(s):  
B A Hosler ◽  
M B Rogers ◽  
C A Kozak ◽  
L J Gudas
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Zinc Finger ◽  
Chromosome 8 ◽  
Lacz Gene ◽  
F9 Cells ◽  
Zinc Finger Gene ◽  
Teratocarcinoma Cells ◽  
Octamer Motif ◽  
F9 Teratocarcinoma

The message for the zinc finger gene Rex-1 (Zfp-42) is expressed in undifferentiated murine F9 teratocarcinoma cells and embryonic stem cells. Expression of Rex-1 is reduced at the transcriptional level when F9 cells are induced by the addition of retinoic acid (RA) to differentiate. We have isolated genomic DNA for the Rex-1 gene (Zfp-42), characterized the gene's structure, and mapped the gene to mouse chromosome 8. Promoter elements contributing to the regulation of the Rex-1 promoter in F9 cells have been identified. A region required for Rex-1 promoter activity in F9 stem cells contains an octamer motif (ATTTGCAT) which is a binding site for octamer transcription factor members of the POU domain family of DNA-binding proteins. Rex-1 reporter plasmids including this octamer site also exhibited reduced expression in F9 cells treated with RA. Thus, the octamer motif is a regulatory element required for the activity of the Rex-1 promoter in F9 stem cells, and this motif contributes to the negative regulation by RA of the transcription of the Rex-1 gene. As an initial confirmation of the in vivo relevance of the isolated fragment, a larger Rex-1 promoter fragment, also containing the octamer site, was able to promote expression of the bacterial lacZ gene in mouse embryos at the morula stage.

Salinomycin-Loaded Gold Nanoparticles for Treating Cancer Stem Cells by Ferroptosis-Induced Cell Death

2019 ◽  
Vol 16 (6) ◽  
pp. 2532-2539 ◽  
Author(s):  
Yongmei Zhao ◽  
Wei Zhao ◽  
Yi Chieh Lim ◽  
Tianqing Liu
Keyword(s):  
Stem Cells ◽  
Gold Nanoparticles ◽  
Cell Death ◽  
Cancer Stem Cells

scholarly journals An octamer motif contributes to the expression of the retinoic acid-regulated zinc finger gene Rex-1 (Zfp-42) in F9 teratocarcinoma cells.

1993 ◽  
Vol 13 (5) ◽  
pp. 2919-2928 ◽  
Author(s):  
B A Hosler ◽  
M B Rogers ◽  
C A Kozak ◽  
L J Gudas
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Zinc Finger ◽  
Chromosome 8 ◽  
Lacz Gene ◽  
F9 Cells ◽  
Zinc Finger Gene ◽  
Teratocarcinoma Cells ◽  
Octamer Motif ◽  
F9 Teratocarcinoma

The message for the zinc finger gene Rex-1 (Zfp-42) is expressed in undifferentiated murine F9 teratocarcinoma cells and embryonic stem cells. Expression of Rex-1 is reduced at the transcriptional level when F9 cells are induced by the addition of retinoic acid (RA) to differentiate. We have isolated genomic DNA for the Rex-1 gene (Zfp-42), characterized the gene's structure, and mapped the gene to mouse chromosome 8. Promoter elements contributing to the regulation of the Rex-1 promoter in F9 cells have been identified. A region required for Rex-1 promoter activity in F9 stem cells contains an octamer motif (ATTTGCAT) which is a binding site for octamer transcription factor members of the POU domain family of DNA-binding proteins. Rex-1 reporter plasmids including this octamer site also exhibited reduced expression in F9 cells treated with RA. Thus, the octamer motif is a regulatory element required for the activity of the Rex-1 promoter in F9 stem cells, and this motif contributes to the negative regulation by RA of the transcription of the Rex-1 gene. As an initial confirmation of the in vivo relevance of the isolated fragment, a larger Rex-1 promoter fragment, also containing the octamer site, was able to promote expression of the bacterial lacZ gene in mouse embryos at the morula stage.

Direct Conjugation of Retinoic Acid with Gold Nanoparticles to Improve Neural Differentiation of Human Adipose Stem Cells

2020 ◽  
Vol 70 (11) ◽  
pp. 1836-1850
Author(s):  
Vajihe Asgari ◽  
Amir Landarani-Isfahani ◽  
Hossein Salehi ◽  
Noushin Amirpour ◽  
Batool Hashemibeni ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gold Nanoparticles ◽  
Retinoic Acid ◽  
Neural Differentiation ◽  
Adipose Stem Cells ◽  
Human Adipose Stem Cells

Butyrate inhibits the retinoic acid-induced differentiation of F9 teratocarcinoma stem cells

1984 ◽  
Vol 105 (2) ◽  
pp. 443-450 ◽  
Author(s):  
Roy A. Levine ◽  
Judith Campisi ◽  
Sho-Ya Wang ◽  
Lorraine J. Gudas
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Induced Differentiation ◽  
F9 Teratocarcinoma
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