scholarly journals Synthesis of Ultrasmall Single-Crystal Gold–Silver Alloy Nanotriangles and Their Application in Photothermal Therapy

Nanomaterials ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 912
Author(s):  
Mirko Maturi ◽  
Erica Locatelli ◽  
Letizia Sambri ◽  
Silvia Tortorella ◽  
Sašo Šturm ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Cell Viability ◽  
Photothermal Therapy ◽  
Near Infrared ◽  
Cell Model ◽  
Cell Viability Assay ◽  
Cell Compatibility ◽  
Viability Assay ◽  
Gold Silver

Photothermal therapy has always been a very attractive anti-cancer strategy, drawing a lot of attention thanks to its excellent performance as a non-invasive and pretty safe technique. Lately, nanostructures have become the main characters of the play of cancer therapy due to their ability to absorb near-infrared radiation and efficient light-to-heat conversion. Here we present the synthesis of polyethylene glycol (PEG)-stabilized hybrid ultrasmall (<20 nm) gold–silver nanotriangles (AuAgNTrs) and their application in photothermal therapy. The obtained AuAgNTrs were deeply investigated using high-resolution transmission electron microscopy (HR-TEM). The cell viability assay was performed on U-87 glioblastoma multiforme cell model. Excellent photothermal performance of AuAgNTrs upon irradiation with NIR laser was demonstrated in suspension and in vitro, with >80% cell viability decrease already after 10 min laser irradiation with a laser power P = 3W/cm2 that was proved to be harmless to the control cells. Moreover, a previous cell viability test had shown that the nanoparticles themselves were reasonably biocompatible: without irradiation cell viability remained high. Herein, we show that our hybrid AuAgNTrs exhibit very exciting potential as nanostructures for hyperthermia cancer therapy, mostly due to their easy synthesis protocol, excellent cell compatibility and promising photothermal features.

Evaluation of Paclitaxel (Taxol), Cisplatin, and the Combination Paclitaxel-Cisplatin in Ovarian Cancer in Vitro with the ATP Cell Viability Assay

1994 ◽  
Vol 53 (1) ◽  
pp. 44-49 ◽  
Author(s):  
Michael Untch ◽  
Bernd-Uwe Sevin ◽  
James P. Perras ◽  
Roberto Angioli ◽  
Andrea Untch ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Viability ◽  
Cell Viability Assay ◽  
Viability Assay

scholarly journals Efek Sitotoksik Ekstrak Daun Ketapang (Terminalia catappa L.) Pada Sel Kanker Payudara T47D

2018 ◽  
Vol 8 (2) ◽  
Author(s):  
Barinta Widaryanti ◽  
Nur Khikmah ◽  
Nunung Sulistyani
Keyword(s):  
Cancer Therapy ◽  
Cell Viability ◽  
Ethyl Acetate ◽  
Breast Cancer Cells ◽  
Methanol Extract ◽  
Cell Viability Assay ◽  
Key Words Cancer ◽  
T47d Cells ◽  
Viability Assay ◽  
The World

Cancer is the second cause of death in the world after heart disease. Cancer therapy using natural product was considered has no side effect. Secondary metabolites from ketapang (Terminalia cattapa L) leaves has a prospect for cancer therapy. The objective of this research was to determine the cytotoxical effect of ketapang leaves on T47D breast cancer cells. Ketapang leaves were macerated using chloroform, ethyl acetate and methanol. Cytotoxicity effect were determined using MTT Cell Viability Assay. The result showed that chloroform, ethyl acetate and methanol extract were not able to reduce cell viability of T47D cells. Key words: cancer, ketapang, T. cattapa, cytotoxic, T47D cells.

scholarly journals A Novel Synthesis of Fe3O4@SiO2@Au@Porous SiO2 Structure for NIR Irradiation-Induced DOX Release and Cancer Treatment

Dose-Response ◽  
2020 ◽  
Vol 18 (1) ◽  
pp. 155932582090666
Author(s):  
Meng Yang ◽  
Wenhua Yang ◽  
Liang Chen ◽  
Mingjian Ding ◽  
Chenhao Li ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Near Infrared ◽  
Drug Loading ◽  
Thermal Conversion ◽  
Au Nanoparticle ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Transmission Electron ◽  
Adsorption Desorption

Doxorubicin (DOX) alone or in combination has been widely used for numerous cancers, including breast, lung, bladder, and so on. In this article, a core/shell/shell structured Fe3O4@SiO2@Au@porous SiO2 particles for the drug delivery and release of DOX was demonstrated, with the aid of near-infrared irradiation. Fe3O4 was used to direct the transportation and delivery of the drug-loaded composite to the target tissues and organs under an external magnetic field, the first layer of SiO2 was used for Au nanoparticle attachment, Au acted as the agent for light–thermal conversion, and the porous SiO2 was used to load DOX. The morphology of the nanoparticles was studied by transmission electron microscopy, and the porous structure was characterized by N2 adsorption/desorption curves. The drug delivery system displayed high drug loading capacity, and the release behavior was largely impacted by the environmental pH. Furthermore, the cytotoxicity of Fe3O4@SiO2@Au@porous SiO2 and DOX loaded Fe3O4@SiO2@Au@porous SiO2 was studied through in vitro 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cell viability assay.

Evaluation of the Influence of Ferrite Magnetic Nanoparticle for Cancer Cell

2021 ◽  
Vol 323 ◽  
pp. 146-151
Author(s):  
Khishigdemberel Ikhbayar ◽  
Nomin Myagmar ◽  
Gantulga Davaakhuu ◽  
Uyanga Enkhnaran ◽  
Enkhmend Bekhbaatar ◽  
...  
Keyword(s):  
Cell Viability ◽  
Colony Formation ◽  
Magnetic Nanoparticle ◽  
In Vitro Cytotoxicity ◽  
Colony Formation Assay ◽  
Cell Viability Assay ◽  
Cytotoxic Effects ◽  
Viability Assay ◽  
A Cell

Magnetic nanoparticles for thermotherapy must be biocompatible and possess high thermal efficiency as heating elements. The biocompatibility of Mg 0.8 Ni 0.2 Fe 2 O 4 nanoparticles was studied using a cytotoxicity colony formation assay and a cell viability assay. HeLa cells exhibited cytotoxic effects when exposed to three different concentrations of 150 μg /ml, 100 μg /ml, and 50 μg /ml nanoparticles. Therefor e, c oncentrations of 50 μg /ml showed the lowest cytotoxic activity and the lowest toxicity to living cells. In vitro cytotoxicity of samples was then investigated by two methods, colony formation assay and cell viability assay. The Hela inhibited cell growth as 16.8% during heating by magnetic field generators.

Ionic conductive nanocomposite based on poly(l-lactic acid)/poly(amidoamine) dendrimerelectrospun nanofibrous for biomedical application

2021 ◽  
Author(s):  
Paniz Memarian ◽  
Atefeh Solouk ◽  
Zohre Bagher ◽  
Somaye Akbari ◽  
Masoumeh Haghbin Nazarpak
Keyword(s):  
Lactic Acid ◽  
Ionic Conductivity ◽  
Cell Viability ◽  
Biomedical Application ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Scanning Calorimetry ◽  
Cell Viability Assay ◽  
Viability Assay

Abstract The modification of poly (l-lactic acid) (PLLA) electrospun nanofibrous scaffolds was carried out by blending with second-generation poly amidoamine (PAMAM) for enhancement of their ionic conductivity. The samples containing PLLA and various amounts of PAMAM (1%, 3%, 5%, and 7% by wt.) were fabricated by electrospinning techniques. The electrospun fibers were characterized using scanning electron microscopy (SEM), porosity, Fourier-transform infrared (FTIR) spectroscopy, differential scanning calorimetry, contact angle measurement, water uptake measurement, mechanical properties, and electrical properties. Furthermore, in vitro degradation study and cell viability assay were investigated in biomaterial applications. Creating amide groups through aminolysis reaction was confirmed by FTIR analysis successfully. The results reveal that adding PAMAM caused an increase in fiber diameter, crystallinity percentage, hydrophilicity, water absorption, elongation-at-break, and OE-mesenchymal stem cell viability. It is worth mentioning that this is the first report investigating the conductivity of PLLA/PAMAM nanofiber. The results revealed that by increasing the amount of PAMAM, the ionic conductivity of scaffolds was enhanced by about nine times. Moreover, the outcomes indicated that the presence of PAMAM could improve the limitations of PLLA like hydrophobicity, lack of active group, and poor cell adhesion.

scholarly journals Antihistamines and Ovarian Cancer Survival: Nationwide Cohort Study and in Vitro Cell Viability Assay

2019 ◽  
Vol 112 (9) ◽  
pp. 964-967 ◽  
Author(s):  
Freija Verdoodt ◽  
Christian Dehlendorff ◽  
Marja Jäättelä ◽  
Robert Strauss ◽  
Anton Pottegård ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Death ◽  
Cohort Study ◽  
Cell Viability ◽  
Cancer Patients ◽  
Cancer Mortality ◽  
Specific Cell ◽  
Cell Viability Assay ◽  
Viability Assay

Abstract Antihistamines with cationic amphiphilic drug (CAD) characteristics induce cancer-specific cell death in experimental studies. Epidemiologic evidence is, however, limited. In a Danish nationwide cohort of ovarian cancer patients diagnosed during 2000–2015 (n = 5075), we evaluated the association between filled antihistamine prescriptions and cancer mortality. We used Cox regression models to estimate hazard ratios (HRs) with 95% confidence intervals (CIs) for ovarian cancer mortality. In an in vitro cell viability assay, we evaluated cell death in three ovarian cancer cell lines after treatment with clinically relevant doses of eight antihistamines. In our cohort study, CAD antihistamine use (≥1 prescription; n = 133) was associated with a hazard ratio of 0.63 (95% CI = 0.40 to 0.99) compared to use of non-CAD antihistamines (n = 304), and we found a tendency toward a dose-response association. In our cell viability assay, we found consistent and dose-dependent cytotoxicity for all CAD but not non-CAD antihistamines. In this nationwide cohort study, use of antihistamines with CAD characteristics is associated with a prognostic benefit in ovarian cancer patients.

scholarly journals Influence of Vanadium 4+ and 5+ Ions on the Differentiation and Activation of Human Osteoclasts

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Matthias A. König ◽  
Oliver P. Gautschi ◽  
Hans-Peter Simmen ◽  
Luis Filgueira ◽  
Dieter Cadosch
Keyword(s):  
Cell Viability ◽  
Treatment Options ◽  
Implant Failure ◽  
Tartrate Resistant Acid Phosphatase ◽  
Negative Effects ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Multinucleated Cells ◽  
And Function

Background. In the pathophysiology of implant failure, metal ions and inflammation-driven osteoclasts (OC) play a crucial role. The aim of this study was to investigate whether vanadium (V) ions induce differentiation of monocytic OC precursors into osteoresorptive multinucleated cells. In addition, the influence of V ions on the activation and function of in vitro generated OC was observed. Methods. Human monocytes and osteoclasts were isolated from peripheral blood monocytic cells (PBMCs). Exposition with increasing concentrations (0–3 μM) of V4+/V5+ ions for 7 days followed. Assessment of OC differentiation, cell viability, and resorptional ability was performed by standard colorimetric cell viability assay 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenil)-2H-tetrazolium (MTS), tartrate-resistant acid phosphatase (TRAP) expression, and functional resorption assays on bone slides during a period of 21 days. Results. No significant differences were noted between V4+/V5+ ions (p>0.05). MTS showed significant reduction in cellular viability by V concentrations above 3 μM (p<0.05). V concentrations above 0.5 μM showed negative effects on OC activation/differentiation. Higher V concentrations showed negative effects on resorptive function (all p<0.05) without affecting cell viability. V4+/V5+ concentrations below 3 μM have negative effects on OC differentiation/function without affecting cell survival. Conclusion. Vanadium-containing implants may reduce implant failure rate by influencing osteoclast activity at the bone-implant interface. V-ligand complexes might offer new treatment options by accumulating in the bone.

Sensitivity of Oncogenic KRAS-Expressing Cells to CDK9 Inhibition

2021 ◽  
pp. 247255522110088
Author(s):  
Lick Pui Lai ◽  
Viviane Brel ◽  
Kanika Sharma ◽  
Julia Frappier ◽  
Nadia Le-Henanf ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cell Lines ◽  
Specific Activity ◽  
Mammalian Target Of Rapamycin ◽  
Mouse Embryonic Fibroblast ◽  
Wild Type ◽  
Compound Library ◽  
Cell Viability Assay ◽  
Viability Assay

Oncogenic forms of KRAS proteins are known to be drivers of pancreatic, colorectal, and lung cancers. The goal of this study is to identify chemical leads that inhibit oncogenic KRAS signaling. We first developed an isogenic panel of mouse embryonic fibroblast (MEF) cell lines that carry wild-type RAS, oncogenic KRAS, and oncogenic BRAF. We validated these cell lines by screening against a tool compound library of 1402 annotated inhibitors in an adenosine triphosphate (ATP)-based cell viability assay. Subsequently, this MEF panel was used to conduct a high-throughput phenotypic screen in a cell viability assay with a proprietary compound library. All 126 compounds that exhibited a selective activity against mutant KRAS were selected and prioritized based on their activities in secondary assays. Finally, five chemical clusters were chosen. They had specific activity against SW620 and LS513 over Colo320 colorectal cancer cell lines. In addition, they had no effects on BRAFV600E, MEK1, extracellular signal-regulated kinase 2 (ERK2), phosphoinositide 3-kinase alpha (PI3Kα), AKT1, or mammalian target of rapamycin (mTOR) as tested in in vitro enzymatic activity assays. Biophysical assays demonstrated that these compounds did not bind directly to KRAS. We further identified the mechanism of action and showed that three of them have CDK9 inhibitory activity. In conclusion, we have developed and validated an isogenic MEF panel that was used successfully to identify RAS oncogenic or wild-type allele-specific vulnerabilities. Furthermore, we identified sensitivity of oncogenic KRAS-expressing cells to CDK9 inhibitors, which warrants future studies of treating KRAS-driven cancers with CDK9 inhibitors.

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