scholarly journals SERS Immunosensor of Array Units Surrounded by Particles: A Platform for Auxiliary Diagnosis of Hepatocellular Carcinoma

Nanomaterials ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 2090
Author(s):  
Mingyu Cheng ◽  
Yongjun Zhang ◽  
Yaxin Wang ◽  
Aonan Zhu ◽  
Lei Chen ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Electric Fields ◽  
Hot Spot ◽  
High Sensitivity ◽  
Fdtd Simulation ◽  
Whole Process ◽  
Surface Enhanced Raman ◽  
Sers Detection ◽  
Enhanced Raman Scattering ◽  
Different Diameters

Hepatocellular carcinoma (HCC) is one of the diseases with high mortality worldwide, so its early diagnosis and treatment have attracted much attention. Due to the advantages of the high sensitivity of surface-enhanced Raman scattering (SERS) detection, SERS has excellent application value in the diagnosis of HCC. In this paper, silver nanoparticles (AgNPs) are modified by magnetron sputtering on the surface of polystyrene (PS) templates with spheres of two different diameters. The array of units surrounded by particles is successfully prepared and the SERS performance is characterized. The effect of the gap between AgNPs on plasmon coupling and hot spot distribution is discussed. Finite-difference time domain (FDTD) simulation is used to verify the electric fields and hot spot distribution of the array. The differences in the concentrations of HCC markers are analyzed by using the change of SERS signal intensity of the array. The whole process proves that the preparation of structures with a strong local electric field to provide highly sensitive SERS signals is a key link in the detection of HCC markers, which is conducive to the diagnosis of HCC and has potential application value in clinical diagnosis.

Dynamic plasmonic nano-traps for single molecule surface-enhanced Raman scattering

Nanoscale ◽  
2017 ◽  
Vol 9 (30) ◽  
pp. 10694-10700 ◽  
Author(s):  
Yuquan Zhang ◽  
Junfeng Shen ◽  
Zhenwei Xie ◽  
Xiujie Dou ◽  
Changjun Min ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Electric Fields ◽  
Single Molecule ◽  
Surface Enhanced Raman Scattering ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Detection ◽  
Enhanced Raman Scattering ◽  
Molecule Surface

Intense electric fields at the nanoscale are essential for single molecule surface-enhanced Raman scattering (SERS) detection.

scholarly journals Rapid and sensitive biomarker detection using molecular imprinting polymer hydrogel and surface-enhanced Raman scattering

2018 ◽  
Vol 5 (1) ◽  
pp. 171488 ◽  
Author(s):  
Shaona Chen ◽  
Lijing Dong ◽  
Min Yan ◽  
Zhongxu Dai ◽  
Chenghua Sun ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Hot Spot ◽  
High Sensitivity ◽  
Surface Enhanced Raman Scattering ◽  
Test Time ◽  
Time Saving ◽  
Plasmonic Material ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Biomarkers are important biochemical indicators, which could be used for identification, early diagnosis and monitoring of diseases during the course of treatment. However, biomarker diagnosis has some shortcomings such as requiring a large amount of samples, long test time and high cost, which seriously influences the correctness and timely treatment to patients. Here, a relatively fast and efficient plasmonic hot spot-localized surface imprinting of Ag spheres using biomarker template immobilization and hydrogel copolymerization is described. The technique takes a fine control of the imprinting process at the nanometre scale and provides a biosensor with high sensitivity. Proof of the opinion is established by detection of biomarker using surface-enhanced Raman scattering (SERS) spectroscopy. This work represents a valuable step towards SERS with biomarkers for cost-saving and time-saving diagnostic assay. It is expected that the new surface imprinted hydrogel plasmonic material can drive possibilities in advancing application of biomarkers in plasmonic biosensors.

scholarly journals Silica-coated dimers of silver nanospheres as surface-enhanced Raman scattering tags for imaging cancer cells

2013 ◽  
Vol 3 (3) ◽  
pp. 20120092 ◽  
Author(s):  
Xiaohu Xia ◽  
Weiyang Li ◽  
Yu Zhang ◽  
Younan Xia
Keyword(s):  
Raman Scattering ◽  
Cancer Cells ◽  
Hot Spot ◽  
High Sensitivity ◽  
Silica Coating ◽  
Surface Enhanced Raman Scattering ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Sers Tags

Surface-enhanced Raman scattering (SERS) tags have been actively explored as a multiplexing platform for sensitive detection of biomolecules. Here, we report a new type of SERS tags that was fabricated by sequentially functionalizing dimers made of 50 nm Ag nanospheres with 4-mercaptobenzoic acid as the Raman reporter molecule, silica coating as a protective shell and antibody as a targeting ligand. These dimer-based tags give highly enhanced and reproducible Raman signals owing to the presence of a well-defined SERS hot spot at the junction between two Ag nanospheres in the dimer. The SERS enhancement factor (EF) of an individual dimer tag supported on a glass slide can reach a level as high as 4.3 × 10 6 . In comparison, the EFs dropped to 2.8 × 10 5 and 8.7 × 10 5 , respectively, when Ag nanospheres and nanocubes with sizes similar to the spheres in the dimer were used to fabricate the tags using similar procedures. The SERS signals from aqueous suspensions of the dimer-based tags also showed high intensity and good stability. Potential use of the dimer-based tags was demonstrated by imaging cancer cells overexpressing HER2 receptors with good specificity and high sensitivity.

scholarly journals Multilayer Gold-Silver Bimetallic Nanostructures to Enhance SERS Detection of Drugs

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3405 ◽  
Author(s):  
Marta Gambucci ◽  
Elena Cambiotti ◽  
Paola Sassi ◽  
Loredana Latterini
Keyword(s):  
High Sensitivity ◽  
Accurate Information ◽  
Drug Detection ◽  
Drug Molecules ◽  
Gold Silver ◽  
Surface Enhanced Raman ◽  
Sers Detection ◽  
Molecular Specificity ◽  
Enhanced Raman Scattering ◽  
Bimetallic Nanostructures

Surface-enhanced Raman scattering (SERS) is a widely used technique for drug detection due to high sensitivity and molecular specificity. The applicability and selectivity of SERS in the detection of specific drug molecules can be improved by gathering information on the specific interactions occurring between the molecule and the metal surface. In this work, multilayer gold-silver bimetallic nanorods (Au@Ag@AuNRs) have been prepared and used as platforms for SERS detection of specific drugs (namely promethazine, piroxicam, furosemide and diclofenac). The analysis of SERS spectra provided accurate information on the molecular location upon binding and gave some insight into molecule-surface interactions and selectivity in drug detection through SERS.

scholarly journals PROSPECTS AND PROBLEMS OF USING THE EFFECT OF SURFACE-ENHANCED RAMAN SCATTERING IN THE DIAGNOSIS OF VIRAL INFECTIONS

2017 ◽  
pp. 106-114
Author(s):  
O. V. Borisova ◽  
E. B. Fayzuloev ◽  
A. A. Marova ◽  
V. I. Kukushkin ◽  
V. V. Zverev
Keyword(s):  
Raman Scattering ◽  
Viral Infections ◽  
Solid Phase ◽  
High Sensitivity ◽  
Surface Enhanced Raman Scattering ◽  
Clinical Samples ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Detection ◽  
Enhanced Raman Scattering

This review presents the latest advances in the use of surface-enhanced Raman scattering (SERS) immunoassay, which can be used to detect viral markers. As in the case of conventional immunoassays, these methods are often based on «sandwich-type» solid phase immunoassay. In recent years the necessary components of the immunochemical methods with SERS detection is SERS-active substrates to create a variety of approaches have been developed. Despite the difficulty of achieving high sensitivity and specificity in the analysis of clinical samples, a number of successful examples with promising results have been demonstrated.

Novel Ag/Au/Pt trimetallic nanocages used with surface-enhanced Raman scattering for trace fluorescent dye detection

2014 ◽  
Vol 2 (34) ◽  
pp. 5550-5557 ◽  
Author(s):  
Tran Thi Bich Quyen ◽  
Wei-Nien Su ◽  
Ching-Hsiang Chen ◽  
John Rick ◽  
Jyong-Yue Liu ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Hot Spot ◽  
Surface Enhanced Raman Scattering ◽  
Signal Enhancement ◽  
Fluorescent Dye ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Hot Spot Formation ◽  
Spot Formation

Trimetallic nanocages possess inter-metallic synergies among elements and are able to generate significant SERS signal enhancement due to “hot spot” formation.

A sensitive SERS assay for detecting proteins and nucleic acids using a triple-helix molecular switch for cascade signal amplification

2014 ◽  
Vol 50 (66) ◽  
pp. 9409-9412 ◽  
Author(s):  
Sujuan Ye ◽  
Yanying Wu ◽  
Wen Zhang ◽  
Na Li ◽  
Bo Tang
Keyword(s):  
Nucleic Acids ◽  
Signal Amplification ◽  
Triple Helix ◽  
Detection System ◽  
Molecular Switch ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Detection ◽  
Multiple Cycle ◽  
Enhanced Raman Scattering

A sensitive surface-enhanced Raman scattering (SERS) detection system is developed for proteins and nucleic acids based on a triple-helix molecular switch for multiple cycle signal amplification.

scholarly journals Improving SERS Detection of Bacillus thuringiensis Using Silver Nanoparticles Reduced with Hydroxylamine and with Citrate Capped Borohydride

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Hilsamar Félix-Rivera ◽  
Roxannie González ◽  
Gabriela Del Mar Rodríguez ◽  
Oliva M. Primera-Pedrozo ◽  
Carlos Ríos-Velázquez ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Surface Charge ◽  
Near Field ◽  
Medical Diagnostics ◽  
Biological Warfare Agents ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Detection ◽  
Warfare Agents ◽  
Enhanced Raman Scattering

The development of techniques that could be useful in fields other than biological warfare agents countermeasures such as medical diagnostics, industrial microbiology, and environmental applications have become a very important subject of research. Raman spectroscopy can be used in near field or at long distances from the sample to obtain fingerprinting information of chemical composition of microorganisms. In this research, biochemical components of the cell wall and endospores of Bacillus thuringiensis (Bt) were identified by surface-enhanced Raman scattering (SERS) spectroscopy using silver (Ag) nanoparticles (NPs) reduced by hydroxylamine and borohydride capped with sodium citrate. Activation of “hot spots”, aggregation and surface charge modification of the NPs, was studied and optimized to obtain signal enhancements from Bt by SERS. Slight aggregation of the NPs as well as surface charge modification to a more acidic ambient was induced using small-size borohydride-reduced NPs in the form of metallic suspensions aimed at increasing the Ag NP-Bt interactions. Hydroxylamine-reduced NPs required slight aggregation and no pH modifications in order to obtain high spectral quality results in bringing out SERS signatures of Bt.

scholarly journals Ag Nanoparticle-Decorated Cu2S Nanosheets for Surface Enhanced Raman Spectroscopy Detection and Photocatalytic Applications

Nanomaterials ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2508
Author(s):  
Osama Nasr ◽  
Jian-Ru Jiang ◽  
Wen-Shuo Chuang ◽  
Sheng-Wei Lee ◽  
Chih-Yen Chen
Keyword(s):  
Reduction Method ◽  
Organic Pollutant ◽  
Surface Enhanced Raman Spectroscopy ◽  
Highly Sensitive ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sers Detection ◽  
Enhanced Raman Scattering ◽  
New Generation ◽  
Photocatalytic Applications

In this article, we demonstrate a facile, rapid, and practical approach to growing high-quality Cu2S nanosheets decorated with Ag nanoparticles (NPs) through the galvanic reduction method. The Ag/Cu2S nanosheets were efficiently applied to the surface-enhanced Raman scattering (SERS) and photocatalytic degradation applications. The photodegradation of RhB dye with the Ag/Cu2S nanosheets composites occurred at a rate of 2.9 times faster than that observed with the undecorated Cu2S nanosheets. Furthermore, the Ag/Cu2S nanosheets displayed highly sensitive SERS detection of organic pollutant (R6G) as low as 10−9 M. The reproducibility experiments indicated that the Ag/Cu2S nanosheets composites could be used for dual functionality in a new generation of outstandingly sensitive SERS probes for detection and stable photocatalysts.

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