scholarly journals Nickel Nanoparticles Induce the Synthesis of a Tumor-Related Polypeptide in Human Epidermal Keratinocytes

Nanomaterials ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 992
Author(s):  
Javier Jiménez-Lamana ◽  
Simon Godin ◽  
Gerard Aragonès ◽  
Cinta Bladé ◽  
Joanna Szpunar ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Nickel Nanoparticles ◽  
Epidermal Keratinocytes ◽  
Human Epidermal Keratinocytes ◽  
Nickel Allergy ◽  
Skin Cells ◽  
Nickel Binding ◽  
Nickel Compounds ◽  
Linear Behavior ◽  
Genotoxic Carcinogens

Although nickel allergy and carcinogenicity are well known, their molecular mechanisms are still uncertain, thus demanding studies at the molecular level. The nickel carcinogenicity is known to be dependent on the chemical form of nickel, since only certain nickel compounds can enter the cell. This study investigates, for the first time, the cytotoxicity, cellular uptake, and molecular targets of nickel nanoparticles (NiNPs) in human skin cells in comparison with other chemical forms of nickel. The dose-response curve that was obtained for NiNPs in the cytotoxicity assays showed a linear behavior typical of genotoxic carcinogens. The exposure of keratinocytes to NiNPs leads to the release of Ni2+ ions and its accumulation in the cytosol. A 6 kDa nickel-binding molecule was found to be synthesized by cells exposed to NiNPs at a dose corresponding to medium mortality. This molecule was identified to be tumor-related p63-regulated gene 1 protein.

scholarly journals Indirubin attenuates mouse psoriasis-like skin lesion in a CD274-dependent manner: an achievement of RNA sequencing

2018 ◽  
Vol 38 (6) ◽  
Author(s):  
Xiaochun Xue ◽  
Jianhua Wu ◽  
Junhui Li ◽  
Jianguo Xu ◽  
Haiying Dai ◽  
...  
Keyword(s):  
Network Analysis ◽  
Skin Lesion ◽  
Candidate Genes ◽  
Rna Sequencing ◽  
Molecular Mechanisms ◽  
Regulatory Mechanisms ◽  
Epidermal Keratinocytes ◽  
Dependent Manner ◽  
Human Epidermal Keratinocytes ◽  
Ifn Γ

It was previously reported that the expression of CD274 was down-regulated in psoriatic epidermis, leading to immune disorders of psoriasis. However, the regulatory mechanisms of CD274 were rarely elucidated. We aimed to explore the regulatory mechanisms of CD274. Skin samples were collected from 18 patients with psoriasis vulgaris and 9 healthy participants for RNA sequencing. Candidate genes were chosen based on degree and k-core difference of genes in the co-expression network. The relations between candidate genes and CD274 were validated by flow cytometry and real-time PCR in primary human epidermal keratinocytes. The therapeutic effect of indirubin was assessed in an imiquimod-treated mouse model. Interferon-γ (IFN-γ), cyclin-dependent kinase (CDK) 1, Toll-like receptor 3 (TLR3), TLR4 and interleukin (IL)-17A were considered as candidate genes. In primary human epidermal keratinocytes, the level of CD274 was obviously increased under the stimulation of IFN-γ and CDK1 inhibitor (indirubin), independent of TLR4, TLR3 or IL-17A. Indirubin alleviated the severity of psoriatic mice in a CD274-dependent manner. Co-expression network analysis served as an effective method for the exploration of molecular mechanisms. We demonstrated for the first time that CD274 was the regulator of indirubin-mediated effect on mouse psoriasis-like skin lesion based on co-expression network analysis, contributing to the alleviation of mouse psoriasis-like skin lesion.

scholarly journals miR-136 Modulates TGF-β1-Induced Proliferation Arrest by Targeting PPP2R2A in Keratinocytes

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Dianbao Zhang ◽  
Jing Wang ◽  
Zhe Wang ◽  
Tao Zhang ◽  
Ping Shi ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Skin Wound ◽  
Luciferase Reporter ◽  
Epidermal Keratinocytes ◽  
Dependent Manner ◽  
Hacat Cells ◽  
Skin Wound Healing ◽  
Human Epidermal Keratinocytes ◽  
Reporter Assays ◽  
Normal Human

Keratinocytes proliferation is critical for the capacity to heal wounds and accumulating evidences have proved that dysregulation of microRNAs is involved in proliferation of keratinocytes. However, the molecular mechanisms remain to be completely elucidated. Here, we show that miR-136 was significantly decreased by TGF-β1 treatment in HaCaT cells and normal human epidermal keratinocytes (NHEK), and it was a Smad3-dependent manner. By cell proliferation assay and cell cycle analysis, we found that reintroduction of miR-136 by transfection, as well as PPP2R2A silencing, counteracted TGF-β-induced proliferation arrest in HaCaT cells. Further, PPP2R2A was verified as a direct target of miR-136 by dual-luciferase reporter assays and Western blotting. These data suggest that miR-136 may play an important role during TGF-β1-induced proliferation arrest by targeting PPP2R2A in keratinocytes, which might represent a potential target for improving skin wound healing.

scholarly journals Laminarin Effects, a β-(1,3)-Glucan, on Skin Cell Inflammation and Oxidation

Cosmetics ◽  
2020 ◽  
Vol 7 (3) ◽  
pp. 66 ◽  
Author(s):  
Hélène Ozanne ◽  
Hechmi Toumi ◽  
Benoît Roubinet ◽  
Ludovic Landemarre ◽  
Eric Lespessailles ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Antioxidant Activities ◽  
Dermal Fibroblasts ◽  
Epidermal Keratinocytes ◽  
Type I ◽  
Human Epidermal Keratinocytes ◽  
Procollagen Type ◽  
Skin Cells ◽  
Radiation Conditions ◽  
Uva Radiation

Laminarin, a β-(1,3)-glucan from the seaweed Laminaria digitata, is a polysaccharide which provides anti-inflammatory and anti-oxidative properties. Its influence on both human dermal fibroblasts adult (HDFa) and normal human epidermal keratinocytes (NHEK) has not been established yet. Herein, laminarin effects were examined on skin cells’ mitochondrial and antioxidant activities. Cytokines, hyaluronic acid, and procollagen type I secretions and interaction mechanisms were explored after a maximum of 72 h treatment with laminarin. Our results demonstrated a decrease in mitochondrial activities with 72 h treatment with laminarin from 500 µg.mL−1 for NHEK cells and from 100 µg.mL−1 for HDFa cells without cytotoxicity. No variation of hyaluronic acid or type I procollagen was observed for all laminarin concentrations, while an antioxidant effect was found against reactive oxygen species (ROS) from 1 µg.mL−1 for HDFa cells in both H2O2 and UVA radiation conditions, and from 10 µg.mL−1 and 1 µg.mL−1 for NHEK cells in both H2O2 and UVA radiation conditions, respectively. Laminarin treatment modulated both cells surface glycosylation and cytokine secretions of skin cells. Overall, our data suggest a positive effect of β-(1,3)-glucan on skin cells on oxidative stress and inflammation induced by environmental factors. Of note, these effects are through the modulation of glycan and receptors interactions at the skin cells surface.

Cytotoxicity of Magnetic Nanoparticles on Normal and Malignant Human Skin Cells

Nano LIFE ◽  
2014 ◽  
Vol 04 (01) ◽  
pp. 1440002 ◽  
Author(s):  
Rehab M. Amin ◽  
Abuelmagd Abdelmonem ◽  
Thomas Verwanger ◽  
Elsayed Elsherbini ◽  
Barbara Krammer
Keyword(s):  
Magnetic Nanoparticles ◽  
Cell Lines ◽  
Dermal Fibroblasts ◽  
Carcinoma Cells ◽  
Diagnostic Tools ◽  
Epidermal Keratinocytes ◽  
A431 Cells ◽  
Human Epidermal Keratinocytes ◽  
Skin Cells ◽  
Human Squamous Cell Carcinoma

Magnetic nanoparticles have received considerable attention in nanomedicine due to their potential application as therapeutic or diagnostic tools based on their particular properties. However, prior to clinical application investigating the effect of these nanoparticles on cells is essential. The aim of the following study is therefore to evaluate the cytotoxicity of magnetic ( Fe 3 O 4) and gold-coated magnetic nanoparticles ( Fe 3 O 4@ Au ) on various cell lines in order to clarify the risk of these materials for human use. Toxicity of these nanoparticles on human dermal fibroblasts (SKIN), human squamous cell carcinoma cells (A431 cells) and human epidermal keratinocytes ( HaCaT cells) were determined using the MTT assay. Results showed that, within the used concentration range, Fe 3 O 4 nanoparticles had no significant effect on all investigated cell lines, while Fe 3 O 4@ Au nanoparticles seem to have a moderate toxicity on all cell lines with some selectivity for the malignant cells, although it is yet moderate. The different characteristic of the cell lines' survival with respect to incubation time and nanoparticle concentration could be partly due to different cell death modes. Therefore, the prepared Fe 3 O 4 nanoparticles are harmless and could be applied safely for skin cancer treatment or diagnosis.

scholarly journals The Effect of Cannabidiol on UV-Induced Changes in Intracellular Signaling of 3D-Cultured Skin Keratinocytes

2021 ◽  
Vol 22 (3) ◽  
pp. 1501
Author(s):  
Agnieszka Gęgotek ◽  
Sinemyiz Atalay ◽  
Adelina Rogowska-Wrzesińska ◽  
Elżbieta Skrzydlewska
Keyword(s):  
Uv Radiation ◽  
Intracellular Signaling ◽  
De Novo ◽  
Protein Biosynthesis ◽  
Protein Structures ◽  
Epidermal Keratinocytes ◽  
Environmental Damage ◽  
Human Epidermal Keratinocytes ◽  
Skin Cells ◽  
Induced Changes

Human epidermal keratinocytes are constantly exposed to UV radiation. As a result, there is a significant need for safe and effective compounds to protect skin cells against this environmental damage. This study aimed to analyze the effect of phytocannabinoid-cannabinoid (CBD)-on the proteome of UVA/B irradiated keratinocytes. The keratinocytes were cultured in a three-dimensional (3D) system, designed to mimic epidermal conditions closely. The obtained results indicate that CBD protected against the harmful effects of UVA/B radiation. CBD decreased the expression of proinflammatory proteins, including TNFα/NFκB and IκBKB complex and decreased the expression of proteins involved in de novo protein biosynthesis, which are increased in UVA/B-irradiated cells. Additionally, CBD enhanced the UV-induced expression of 20S proteasome subunits. CBD also protected protein structures from 4-hydroxynonenal (HNE)-binding induced by UV radiation, which primarily affects antioxidant enzymes. CBD-through its antioxidant/anti-inflammatory activity and regulation of protein biosynthesis and degradation-protects skin cells against UVA/B-induced changes. In the future, its long-term use in epidermal cells should be investigated.

scholarly journals Red Raspberry Extract Protects the Skin against UVB-Induced Damage with Antioxidative and Anti-inflammatory Properties

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Pei-Wen Wang ◽  
Yu-Chen Cheng ◽  
Yu-Chiang Hung ◽  
Chih-Hung Lee ◽  
Jia-You Fang ◽  
...  
Keyword(s):  
Nude Mouse ◽  
Molecular Mechanisms ◽  
Target Genes ◽  
Inflammatory Responses ◽  
Mouse Skin ◽  
Epidermal Keratinocytes ◽  
Uvb Radiation ◽  
Red Raspberry ◽  
Human Epidermal Keratinocytes ◽  
Nude Mouse Model

Extensive exposure to UVB (280–320 nm) is the major risk responsible for various skin injuries. Numerous reports have shown that natural products could demonstrate photochemopreventive efficacy against UVB damage. We investigated the preventive effects and associated molecular mechanisms of red raspberry extract upon UVB-caused damage in human epidermal keratinocytes and a nude mouse model. The protein profiles and immunohistological study on a nude mouse skin indicated that red raspberry extract could prevent UVB-caused cell death and protect the skin against UVB-exposed injury manifested by wrinkling, scaling, tanning, and water loss as well as epidermal thickening. In addition, red raspberry extract application effectively abolished oxidative damage in DNA and attenuated the carbonylation level of proteins, which attributed to the activation of SOD, Nrf2 and its target genes, and HO-1. Red raspberry extract also altered the cells’ apoptotic signaling pathways including caspase-3 as well as the inflammatory cascade such as c-jun and attenuated UVB-induced activation of NF-κB and COX-2. Red raspberry extract could alleviate direct photodamage to the skin caused by UVB exposure through the ROS scavenger and protection against inflammatory responses, which may allow the development of novel strategies in protecting the skin subjected to UVB radiation.

Alterations of ultrastructure and elemental composition in cultured human epidermal keratinocytes after treatment with nitrofurazone and silver sulfadiazine

1987 ◽  
Vol 45 ◽  
pp. 676-677
Author(s):  
A. R. Crooker ◽  
M. C. Myers ◽  
T. L. Beard ◽  
E. S. Graham
Keyword(s):  
Electron Microscopy ◽  
Elemental Composition ◽  
Pyrolytic Carbon ◽  
Human Keratinocytes ◽  
Silver Sulfadiazine ◽  
Epidermal Keratinocytes ◽  
Human Epidermal Keratinocytes ◽  
Culture Systems ◽  
Cytotoxicity Endpoints

Cell culture systems have become increasingly popular as a means of screening toxic agents and studying toxic mechanisms of drugs and other chemicals at the cellular and subcellular levels. These in vitro tests can be conducted rapidly in a broad range of relevant mammalian culture systems; a variety of biological and biochemical cytotoxicity endpoints can be examined. The following study utilized human keratinocytes to evaluate the relative cytotoxicities of nitrofurazone (NF) and silver sulfadiazine (SS), the active ingredients of FURACIN(R) Topical Cream and SILVADENE(R) Cream, respectively. These compounds are anti-infectives used in the treatment of burn patients. Cell ultrastructure and elemental composition were utilized as cytotoxicity endpoints.Normal Human Epidermal Keratinocytes (HK) were prepared from the EpiPackTM culture system (Clonetics Corporation, Boulder, CO). For scanning electron microscopy (SEM) and transmission electron microscopy (TEM), cells were seeded on sterile 35 mm Falcon plastic dishes; for elemental microanalysis, cells were plated on polished pyrolytic carbon discs (E. Fullam, Latham, NY) placed in the culture dishes.

scholarly journals Antioxidant and Pro-Oxidant Capacities as Mechanisms of Photoprotection of Olive Polyphenols on UVA-Damaged Human Keratinocytes

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2153
Author(s):  
Raffaella Marina Lecci ◽  
Isabella D’Antuono ◽  
Angela Cardinali ◽  
Antonella Garbetta ◽  
Vito Linsalata ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Human Keratinocytes ◽  
Ldl Oxidation ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Epidermal Keratinocytes ◽  
Olive Cultivar ◽  
Human Epidermal Keratinocytes ◽  
Apoptotic Effect

A wide variety of polyphenols are reported to have considerable antioxidant and skin photoprotective effects, although the mechanisms of action are not fully known. Environmentally friendly and inexpensive sources of natural bioactive compounds, such as olive mill wastewater (OMWW), the by-product of olive-oil processing, can be considered an economic source of bioactive polyphenols, with a range of biological activities, useful as chemotherapeutic or cosmeceutical agents. Green strategies, such as the process based on membrane technologies, allow to recover active polyphenols from this complex matrix. This study aims to evaluate the antioxidant, pro-oxidant, and photoprotective effects, including the underlying action mechanism(s), of the ultra-filtered (UF) OMWW fractions, in order to substantiate their use as natural cosmeceutical ingredient. Six chemically characterized UF-OMWW fractions, from Italian and Greek olive cultivar processing, were investigated for their antioxidant activities, measured by Trolox Equivalent Antioxidant Capacity (TEAC), LDL oxidation inhibition, and ROS-quenching ability in UVA-irradiated HEKa (Human Epidermal Keratinocytes adult) cultures. The photoprotective properties of UF-OMWW were assayed as a pro-oxidant-mediated pro-apoptotic effect on the UVA-damaged HEKa cells, which can be potentially involved in the carcinogenesis process. All the UF-OMWW fractions exerted an effective antioxidant activity in vitro and in cells when administered together with UV-radiation on HEKa. A pro-oxidative and pro-apoptotic effect on the UVA-damaged HEKa cells were observed, suggesting some protective actions of polyphenol fraction on keratinocyte cell cultures.

scholarly journals 514 Uvb-induced necrosis in human epidermal keratinocytes

2021 ◽  
Vol 141 (5) ◽  
pp. S90
Author(s):  
H. Plunkett ◽  
M.F. Denning ◽  
M. Mifsud
Keyword(s):  
Epidermal Keratinocytes ◽  
Human Epidermal Keratinocytes
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