scholarly journals Detecting Oxidative Stress Biomarkers in Neurodegenerative Disease Models and Patients

2020 ◽  
Vol 3 (4) ◽  
pp. 66
Author(s):  
Yulia Sidorova ◽  
Andrii Domanskyi
Keyword(s):  
Oxidative Stress ◽  
Animal Models ◽  
Defense Mechanisms ◽  
Oxidative Stress Markers ◽  
Oxidative Stress Biomarkers ◽  
Simple Method ◽  
Neuronal Populations ◽  
Stress Markers ◽  
Preclinical Animal Models ◽  
Detection And Localization

Oxidative stress is prominent in many neurodegenerative diseases. Along with mitochondrial dysfunction and pathological protein aggregation, increased levels of reactive oxygen and nitrogen species, together with impaired antioxidant defense mechanisms, are frequently observed in Alzheimer’s, Parkinson’s, Huntington’s disease and amyotrophic lateral sclerosis. The presence of oxidative stress markers in patients’ plasma and cerebrospinal fluid may aid early disease diagnoses, as well as provide clues regarding the efficacy of experimental disease-modifying therapies in clinical trials. In preclinical animal models, the detection and localization of oxidatively damaged lipids, proteins and nucleic acids helps to identify most vulnerable neuronal populations and brain areas, and elucidate the molecular pathways and the timeline of pathology progression. Here, we describe the protocol for the detection of oxidative stress markers using immunohistochemistry on formaldehyde-fixed, paraffin-embedded tissue sections, applicable to the analysis of postmortem samples and tissues from animal models. In addition, we provide a simple method for the detection of malondialdehyde in tissue lysates and body fluids, which is useful for screening and the identification of tissues and structures in the nervous system which are most affected by oxidative stress.

scholarly journals Oxidative Stress Implications in the Affective Disorders: Main Biomarkers, Animal Models Relevance, Genetic Perspectives, and Antioxidant Approaches

2016 ◽  
Vol 2016 ◽  
pp. 1-25 ◽  
Author(s):  
Ioana Miruna Balmus ◽  
Alin Ciobica ◽  
Iulia Antioch ◽  
Romeo Dobrin ◽  
Daniel Timofte
Keyword(s):  
Oxidative Stress ◽  
Animal Models ◽  
Affective Disorders ◽  
Dependent Manner ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Oxidative Balance ◽  
Oxidative Stress Status ◽  
Collateral Effect ◽  
Central Nervous System Impairment

The correlation between the affective disorders and the almost ubiquitous pathological oxidative stress can be described in a multifactorial way, as an important mechanism of central nervous system impairment. Whether the obvious changes which occur in oxidative balance of the affective disorders are a part of the constitutive mechanism or a collateral effect yet remains as an interesting question. However it is now clear that oxidative stress is a component of these disorders, being characterized by different aspects in a disease-dependent manner. Still, there are a lot of controversies regarding the relevance of the oxidative stress status in most of the affective disorders and despite the fact that most of the studies are showing that the affective disorders development can be correlated to increased oxidative levels, there are various studies stating that oxidative stress is not linked with the mood changing tendencies. Thus, in this minireview we decided to describe the way in which oxidative stress is involved in the affective disorders development, by focusing on the main oxidative stress markers that could be used mechanistically and therapeutically in these deficiencies, the genetic perspectives, some antioxidant approaches, and the relevance of some animal models studies in this context.

scholarly journals Protective Role of Probiotic Supplements in Hepatic Steatosis: A Rat Model Study

2020 ◽  
Vol 2020 ◽  
pp. 1-15 ◽  
Author(s):  
Aein Azarang ◽  
Omid Farshad ◽  
Mohammad Mehdi Ommati ◽  
Akram Jamshidzadeh ◽  
Reza Heidari ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hepatic Steatosis ◽  
Bacillus Coagulans ◽  
Male Rats ◽  
Health Priorities ◽  
Oxidative Stress Markers ◽  
Oxidative Stress Biomarkers ◽  
Nonalcoholic Fatty Liver ◽  
Stress Markers ◽  
And Oxidative Stress

Background. Treating nonalcoholic fatty liver disease (NAFLD) is considered one of the public health priorities in the past decade. So far, probiotics have represented promising results in controlling the signs and symptoms of NAFLD. However, attempts to find the ideal probiotic strain are still ongoing. The present study is designed to find the best strain amongst suitable probiotic strains according to their ability to ameliorate histopathological and oxidative stress biomarkers in hepatic steatosis-induced rats. Methods. Initially, four probiotics species, including Lactobacillus (L.) acidophilus, L. casei, L. reuteri, and Bacillus coagulans, were cultured and prepared as a lyophilized powder for animals. The experiment lasted for fifty days. Initially, hepatic steatosis was induced by excessive ingestion of D-fructose in rats for eight weeks, followed by eight weeks of administering probiotics and D-fructose concurrently. Forty-two six-week-old male rats were alienated to different groups and were supplemented with different probiotics ( 1 ∗ 10 9   CFU in 500 mL drinking water). After eight weeks, blood and liver samples were taken for further evaluation, and plasma and oxidative stress markers corresponding to liver injuries were examined. Results. Administration of probiotics over eight weeks reversed hepatic and blood triglyceride concentration and blood glucose levels. Also, probiotics significantly suppressed markers of oxidative stress in the liver tissue. Conclusions. Although some of the single probiotic formulations were able to mitigate oxidative stress markers, mixtures of probiotics significantly ameliorated more symptoms in the NAFLD animals. This enhanced effect might be due to probiotics’ cumulative potential to maintain oxidative stress and deliver improved lipid profiles, liver function markers, and inflammatory markers.

Impairment of antioxidant defense mechanisms in elderly women without increase in oxidative stress markers: “A weak equilibrium”

Lipids ◽  
1999 ◽  
Vol 34 (S1) ◽  
pp. S289-S289 ◽  
Author(s):  
J. P. Cristol ◽  
M. Abderrazick ◽  
F. Favier ◽  
F. Michel ◽  
J. Castel ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Defense Mechanisms ◽  
Antioxidant Defense ◽  
Elderly Women ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Weak Equilibrium

scholarly journals Proinflammatory and Oxidative Stress Markers in Patients with Periodontal Disease

2007 ◽  
Vol 2007 ◽  
pp. 1-5 ◽  
Author(s):  
Ivan Borges Jr. ◽  
Emília Addison Machado Moreira ◽  
Danilo Wilhem Filho ◽  
Tiago Bittencourt de Oliveira ◽  
Marcelo Barreto Spirelle da Silva ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Periodontal Disease ◽  
Gingival Tissue ◽  
Control Group ◽  
Myeloperoxidase Activity ◽  
Enzymatic Antioxidants ◽  
Oxidative Stress Markers ◽  
Oxidative Stress Biomarkers ◽  
Stress Markers ◽  
And Oxidative Stress

Objective. To evaluate the involvement of proinflammatory and oxidative stress markers in gingival tissue in individuals with chronic periodontitis.Subject and methods. Eighteen subjects were divided in two groups: experimental (age52.9±5.0) and control (age51.1±9.6). The activities of enzymatic antioxidants such as catalase, glutathione peroxidase (GPx), glutathione S-transferase (GST), glutathione reductase, nonenzymatic antioxidants: total glutathione and reduced glutathione, oxidized glutathione (GSSG), thiobarbituric acid reactive substances (TBARS), and myeloperoxidase activity (MPO) were evaluated in gingival tissues from interproximal sites. Statistical differences between groups were determined by independent Studentttest andP<.05.Results. Individuals with periodontal disease exhibited a significant increase in the activities of MPO, GPx, GST, and also in TBARS and GSSG levels in gingival tissue compared to the control group (P<.05).Conclusion. The results of the present work showed an important correlation between oxidative stress biomarkers and periodontal disease.

Peripheral Oxidative Stress Markers in Patients with Bipolar Disorder during Euthymia and in Siblings

2020 ◽  
Vol 20 (1) ◽  
pp. 77-86 ◽  
Author(s):  
Amparo Tatay-Manteiga ◽  
Vicent Balanzá-Martínez ◽  
Giovana Bristot ◽  
Rafael Tabarés-Seisdedos ◽  
Flavio Kapczinski ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Bipolar Disorder ◽  
Valproic Acid ◽  
Uric Acid ◽  
Antioxidant Capacity ◽  
Oxidative Stress Markers ◽  
Oxidative Stress Biomarkers ◽  
Protein Carbonyl Content ◽  
Stress Biomarkers ◽  
Stress Markers

Aims:Oxidative stress is increased during the acute phases of bipolar disorder (BD). Our aim here was to analyze oxidative stress biomarkers in patients with BD during euthymia and their siblings.Method:A cross-sectional study was performed in euthymic patients with BD-I (n=48), unaffected siblings (n=23) and genetically unrelated healthy controls (n=21). Protein carbonyl content (PCC), total antioxidant capacity (TRAP), lipid peroxidation (TBARS) and uric acid were measured as biomarkers of oxidative stress in blood.Results:The antioxidant capacity (TRAP) was lower (p<0.001) in patients with BD compared to their siblings and controls, whereas no differences were observed in PCC, TBARS or uric acid. In patients, the concentrations of TRAP and TBARS were positively associated with the dose of valproic acid (p<0.05 and p<0.001, respectively). The concentrations of these biomarkers were not significantly associated with any of socio-demographic and clinical variables.Conclusion:A selective reduction in antioxidant capacity is present in BD during euthymia state, whereas other markers of oxidative stress are unaltered during euthymia. Siblings did not show any alterations in oxidative stress biomarkers. Oxidative stress might represent a state-dependent marker in BD. The association between treatment with valproic acid and oxidative stress markers in euthymia deserves further studies.

scholarly journals Evaluating the protective potency of Acacia hydaspica R. Parker on histological and biochemical changes induced by Cisplatin in the cardiac tissue of rats

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Tayyaba Afsar ◽  
Suhail Razak ◽  
Ali Almajwal ◽  
Maria Shabbir ◽  
Muhammad Rashid Khan
Keyword(s):  
Oxidative Stress ◽  
Creatine Kinase ◽  
Antioxidant Enzymes ◽  
Cardiac Function ◽  
Cardiac Tissue ◽  
Troponin I ◽  
Antioxidant Properties ◽  
Oxidative Stress Markers ◽  
Oxidative Stress Biomarkers ◽  
Stress Markers

Abstract Background Increase oxidative trauma is the main cause behind Cisplatin (CP) induced cardiotoxicity which restricts its clinical application as anti-neoplastic prescription. Acacia hydaspica is a natural shrub with diverse bioactivities. Acacia hydaspica ethyl acetate extract (AHE) ameliorated drug-induced cardiotoxicity in animals with anti-oxidative mechanisms. Current study aimed to evaluate the protective potential of A. hydaspica against cisplatin-induced myocardial injury. Methods Rats were indiscriminately distributed into six groups (n = 6). Group 1: control; Groups 2: Injected with CP (7.5 mg/kg bw, i.p, single dose) on day 16; Group 3: Treated for 21 days with AHE (400 mg/kg b.w, oral); Group 4: Received CP injection on day 16 and treated with AHE for 5 days post injection; Group 5: Received AHE (400 mg/kg b.w/day, p.o.) for 21 days and CP (7.5 mg/kg b.w., i.p.) on day 16; Group 6: Treated with silymarin (100 mg/kg b.w., p.o.) after 1 day interval for 21 days and CP injection (7.5 mg/kg b.w., i.p.) on day 16. On 22nd day, the animals were sacrificed and their heart tissues were removed. Cisplatin induced cardiac toxicity and the influence of AHE were evaluated by examination of serum cardiac function markers, cardiac tissue antioxidant enzymes, oxidative stress markers and histology. Results CP inoculation considerably altered cardiac function biomarkers in serum and diminished the antioxidant enzymes levels, while increased oxidative stress biomarkers in cardiac tissues AHE treatment attenuated CP-induced deteriorations in creatine kinase (CK), Creatine kinase isoenzymes MB (CK-MB), cardiac Troponin I (cTNI) and lactate dehydrogenase (LDH) levels and ameliorated cardiac oxidative stress markers as evidenced by decreasing lipid peroxidation, H2O2 and NO content along with augmentation in phase I and phase II antioxidant enzymes. Additionally, CP inoculation also induced morphological alterations which were ameliorated by AHE. In pretreatment group more significant protection was observed compared to post-treatment group indicating preventive potential of AHE. The protective potency of AHE was comparable to silymarin. Conclusion Results demonstrate that AHE attenuated CP induce cardiotoxicity. The polyphenolic metabolites and antioxidant properties of AHE might be responsible for its protective influence.

PSXV-30 Late-Breaking: Oxidative stress biomarkers in blood plasma and pre-rigor tissues of beef cattle induced by hydrogen peroxide

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 379-380
Author(s):  
Chelsie B Dahlgren ◽  
Dishnu Sajeev ◽  
Thu Dinh ◽  
Daniel Rivera ◽  
Hunter Goodson
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Blood Plasma ◽  
Fixed Effects ◽  
Oxidative Stress Markers ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Stress Markers ◽  
Muscle Tissues ◽  
In Blood Plasma

Abstract This study examined the oxidative stress biomarkers in blood plasma and pre-rigor tissues of beef heifers induced by hydrogen peroxide. Cattle (365 ± 38 kg; n = 18) were supplemented with ground corn and soybean hulls while grazing cool and warm-season pastures, then shipped and finished at a commercial feedlot in Iowa. Animals were blocked into three groups based on initial clusters of oxidative stress markers. Two treatments of either 20 mg hydrogen peroxide/kg BW (OX, n = 9) or 10 mL of saline (CON, n = 9) were equally and randomly assigned to animals within each group. On the day before slaughter, the OX and CON treatments were administered intravenously through the jugular vein. Blood samples were collected immediately before (T0) and 90 min after (T90) treatment and centrifuged into plasma and pre-rigor muscles were collected at the neck (splenius) during slaughter; both were frozen in liquid nitrogen and stored at -80ºC. Blood plasma and muscle tissues were analyzed for total antioxidant capacity (TAC), thiobarbituric acid reactive substances (TBARS), and glutathione peroxidase activity (GPx). Muscle tissues were also analyzed for glutathione (GSH). The data were analyzed as a randomized complete block design with repeated measures with treatment, time, and their interaction as fixed effects. Oxidative stress treatment did not affect TAC and TBARS values (P ≥ 0.137). However, T0 plasma had 0.47 mM less (P = 0.009) and 5.84 μM more (P &lt; 0.001) of TAC and TBARS, respectively, than T90 plasma. Although CON had 27.73 nmol/min/mL more GPx at T0 (P = 0.039), such activity did not differ in both groups at T90 (P = 0.542). Treatment did not affect oxidative stress markers in pre-rigor tissues (P ≥ 0.184). Inducing oxidative stress by hydrogen peroxide had minimal effects on biomarkers in blood and muscle tissues.

Oxidative stress markers in women with polycystic ovary syndrome without insulin resistance

2018 ◽  
Author(s):  
Reveka Gyftaki ◽  
Sofia Gougoura ◽  
Nikolaos Kalogeris ◽  
Vasiliki Loi ◽  
George Koukoulis ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Insulin Resistance ◽  
Polycystic Ovary Syndrome ◽  
Polycystic Ovary ◽  
Oxidative Stress Markers ◽  
Ovary Syndrome ◽  
Stress Markers
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