scholarly journals An Optimised Step-by-Step Protocol for Measuring Relative Telomere Length

2020 ◽  
Vol 3 (2) ◽  
pp. 27 ◽  
Author(s):  
Mugdha V. Joglekar ◽  
Sarang N. Satoor ◽  
Wilson K.M. Wong ◽  
Feifei Cheng ◽  
Ronald C.W. Ma ◽  
...  
Keyword(s):  
Telomere Length ◽  
Large Population ◽  
Single Copy ◽  
Population Based ◽  
Fragment Analysis ◽  
Real Time Quantitative Pcr ◽  
Disease States ◽  
Relative Telomere Length ◽  
Health And Disease

Telomeres represent the nucleotide repeat sequences at the ends of chromosomes and are essential for chromosome stability. They can shorten at each round of DNA replication mainly because of incomplete DNA synthesis of the lagging strand. Reduced relative telomere length is associated with aging and a range of disease states. Different methods such as terminal restriction fragment analysis, real-time quantitative PCR (qPCR) and fluorescence in situ hybridization are available to measure telomere length; however, the qPCR-based method is commonly used for large population-based studies. There are multiple variations across qPCR-based methods, including the choice of the single-copy gene, primer sequences, reagents, and data analysis methods in the different reported studies so far. Here, we provide a detailed step-by-step protocol that we have optimized and successfully tested in the hands of other users. This protocol will help researchers interested in measuring relative telomere lengths in cells or across larger clinical cohort/study samples to determine associations of telomere length with health and disease.

scholarly journals Telomere length: population epidemiology and concordance in Australian children aged 11–12 years and their parents

BMJ Open ◽  
2019 ◽  
Vol 9 (Suppl 3) ◽  
pp. 118-126 ◽  
Author(s):  
Minh Thien Nguyen ◽  
Kate Lycett ◽  
Regan Vryer ◽  
David P Burgner ◽  
Sarath Ranganathan ◽  
...  
Keyword(s):  
Telomere Length ◽  
Globin Gene ◽  
Venous Blood ◽  
Single Copy ◽  
Population Based ◽  
Cross Sectional Study ◽  
Telomeric Dna ◽  
Cross Sectional ◽  
Relative Telomere Length ◽  
Parent Child

ObjectivesTo (1) describe the epidemiology of child and adult telomere length, and (2) investigate parent–child telomere length concordance.DesignPopulation-based cross-sectional study within the Longitudinal Study of Australian Children.SettingAssessment centres in seven major Australian cities and eight selected regional towns; February 2015 to March 2016.ParticipantsOf 1874 participating families, telomere data were available for analysis for 1206 children and 1343 parents, of whom 1143 were parent–child pairs. There were 589 boys and 617 girls; 175 fathers and 1168 mothers.Outcome measuresRelative telomere length (T/S ratio), calculated by comparing telomeric DNA (T) level with the single copy (S) beta-globin gene in venous blood-derived genomic DNA by quantitative real-time PCR.ResultsMean T/S ratio for all children, boys and girls was 1.09 (SD 0.56), 1.05 (SD 0.53) and 1.13 (SD 0.59), respectively. Mean T/S ratio for all parents, fathers and mothers was 0.81 (SD 0.37), 0.82 (SD 0.36) and 0.81 (SD 0.38), respectively. Parent–child T/S ratio concordance was moderate (correlation 0.24). In adjusted regression models, one unit higher parent T/S ratio was associated with 0.36 (estimated linear regression coefficient (β); 95% CI 0.28 to 0.45) higher child T/S ratio. Concordance was higher in the youngest parent-age tertile (β 0.49; 95% CI 0.34 to 0.64) compared with the middle (β 0.35; 95% CI 0.21 to 0.48) and oldest tertile (β 0.26; 95% CI 0.11 to 0.41; p-trend 0.04). Father–child concordance was 0.34 (95% CI 0.18 to 0.48), while mother–child was 0.22 (95% CI 0.17 to 0.28).ConclusionsWe provide telomere length population values for children aged 11–12 years and their mid-life parents. Relative telomere length was shorter in adults than children, as expected. There was modest evidence of parent–child concordance, which diminished with increasing parent age.

scholarly journals Exceptionally Long-Lived Individuals (ELLI) Demonstrate Slower Aging Rate Calculated by DNA Methylation Clocks as Possible Modulators for Healthy Longevity

2020 ◽  
Vol 21 (2) ◽  
pp. 615
Author(s):  
Danielle Gutman ◽  
Elina Rivkin ◽  
Almog Fadida ◽  
Lital Sharvit ◽  
Vered Hermush ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Telomere Length ◽  
Single Copy ◽  
Phenotypic Characterization ◽  
Single Copy Gene ◽  
Online Tool ◽  
Healthy Longevity ◽  
Relative Telomere Length ◽  
Dna Methylation Profile ◽  
Copy Gene

Exceptionally long-lived individuals (ELLI) who are the focus of many healthy longevity studies around the globe are now being studied in Israel. The Israeli Multi-Ethnic Centenarian Study (IMECS) cohort is utilized here for assessment of various DNA methylation clocks. Thorough phenotypic characterization and whole blood samples were obtained from ELLI, offspring of ELLI, and controls aged 53–87 with no familial exceptional longevity. DNA methylation was assessed using Illumina MethylationEPIC Beadchip and applied to DNAm age online tool for age and telomere length predictions. Relative telomere length was assessed using qPCR T/S (Telomere/Single copy gene) ratios. ELLI demonstrated juvenile performance in DNAm age clocks and overall methylation measurement, with preserved cognition and relative telomere length. Our findings suggest a favorable DNA methylation profile in ELLI enabling a slower rate of aging in those individuals in comparison to controls. It is possible that DNA methylation is a key modulator of the rate of aging and thus the ELLI DNAm profile promotes healthy longevity.

scholarly journals Effect of Leukocyte Telomere Length on Total and Regional Brain Volumes in a Large Population-Based Cohort

2014 ◽  
Vol 71 (10) ◽  
pp. 1247 ◽  
Author(s):  
Kevin S. King ◽  
Julia Kozlitina ◽  
Roger N. Rosenberg ◽  
Ronald M. Peshock ◽  
Roderick W. McColl ◽  
...  
Keyword(s):  
Telomere Length ◽  
Large Population ◽  
Population Based ◽  
Leukocyte Telomere Length ◽  
Brain Volumes ◽  
Regional Brain

scholarly journals THE EFFECT OF KE PEPTIDE ON THE TELOMERE LENGTH OF PHA-STIMULATED HUMAN LYMPHOCYTES

2019 ◽  
Vol 19 (1S) ◽  
pp. 166-168
Author(s):  
V Kh Khavinson ◽  
N S Linkova ◽  
A A Pendina ◽  
O A Efimova ◽  
A S Koltsova ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Telomere Length ◽  
Blood Lymphocyte ◽  
Human Lymphocytes ◽  
Human Chromosomes ◽  
Middle Aged ◽  
Blood Lymphocytes ◽  
Aged People ◽  
Relative Telomere Length

The goal of the research is to study the effect of KE peptide on telomere length of PHA-stimulated blood lymphocytes of young and middle-aged men. 11 blood lymphocyte samples were enrolled in the analysis. Relative telomere length was measured using fluorescence in situ hybridization with DNA-probes specific to telomere sequences of human chromosomes. 5 cases of significant telomere length alteration were registered. These changes were more frequent registered in middle-aged people: 4 invents cases versus 1. KE peptide increased telomere length in blood lymphocytes of middle-aged people if it was basically low and, vice versa, decreased the basically high telomere length.

scholarly journals Optimization of a Relative Telomere Length Assay by Monochromatic Multiplex Real-Time Quantitative PCR on the LightCycler 480

2016 ◽  
Vol 18 (3) ◽  
pp. 425-437 ◽  
Author(s):  
Anthony Y.Y. Hsieh ◽  
Sara Saberi ◽  
Abhinav Ajaykumar ◽  
Kyle Hukezalie ◽  
Izabella Gadawski ◽  
...  
Keyword(s):  
Telomere Length ◽  
Real Time ◽  
Quantitative Pcr ◽  
Real Time Quantitative Pcr ◽  
Relative Telomere Length

scholarly journals Relative telomere length of the residents of Lviv oblast

1970 ◽  
Vol 21 ◽  
pp. 316-320
Author(s):  
N. L. Huleiuk ◽  
M. Tyrka
Keyword(s):  
Telomere Length ◽  
Whole Blood ◽  
Short Tandem Repeats ◽  
Tandem Repeats ◽  
Single Copy ◽  
Rt Pcr ◽  
Relative Telomere Length ◽  
Low Correlation ◽  
Whole Blood Cells ◽  
Short Tandem

Aim. Telomeres are short, tandem repeats of DNA that cap linear chromosome ends by binding members of the shelterin protein complex to form protective telomere loops. An insufficient number of telomere repeats leads to chromosome uncapping, cell senescence, and death. Aim of this thesis is the analysis of relative telomere length (RTL) in whole blood among residents of Lviv region. Methods. The RTL in the whole blood cells was measured in 86 residents of various age (47 men and 39 women aged 18–72) using quantitative real-time PCR (Cawthon’s method). It is based on the simultaneous amplification of telomeric repeats (T) and a single copy gene (S). Results. There is a tendency to decrease RTL with age. The low correlation between RTL and age can be linked to various factors, including the heterogeneity of telomere length at birth, chronic socioeconomic stress, genetic determinism, sensitivity to exogenous pressures. Women and men did not differ significantly in the rate of RTL shortening. Conclusions. The results indicate the absence of reliable differences relative telomere length in individuals of different sexes. The low correlation between RTL and age can be linked to various factors, including insufficient sample size of people aged over 50 years. So in the future we plan to continue these studies in older people. Keywords: relative telomere length, RT-PCR, age, gender.

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