scholarly journals Characterizing the Anoxic Phenotype of Pseudomonas putida Using a Bioelectrochemical System

2019 ◽  
Vol 2 (2) ◽  
pp. 26 ◽  
Author(s):  
Bin Lai ◽  
Anh Nguyen ◽  
Jens Krömer
Keyword(s):  
Pseudomonas Putida ◽  
High Yield ◽  
Gas Transfer ◽  
Bioelectrochemical System ◽  
Terminal Electron Acceptor ◽  
Industrial Fermentation ◽  
Electrochemical Technology ◽  
Poor Understanding ◽  
Cultivation Technology ◽  
Obligate Aerobe

Industrial fermentation in aerobic processes is plagued by high costs due to gas transfer limitations and substrate oxidation to CO2. It has been a longstanding challenge to engineer an obligate aerobe organism, such as Pseudomonas putida, into an anaerobe to facilitate its industrial application. However, the progress in this field is limited, due to the poor understanding of the constraints restricting its anoxic phenotype. In this paper, we provide a methodological description of a novel cultivation technology for P. putida under anaerobic conditions, using the so-called microbial electrochemical technology within a bioelectrochemical system. By using an electrode as the terminal electron acceptor (mediated via redox chemicals), glucose catabolism could be activated without oxygen present. This (i) provides an anoxic-producing platform for sugar acid production at high yield and (ii) more importantly, enables systematic and quantitative characterizations of the phenotype of P. putida in the absence of molecular oxygen. This unique electrode-based cultivation approach offers a tool to understand and in turn engineer the anoxic phenotype of P. putida and possibly also other obligate aerobes.

scholarly journals Swasembada Kedelai: Antara Harapan dan Kenyataan

2016 ◽  
Vol 28 (1) ◽  
pp. 55 ◽  
Author(s):  
Gelar Satya Budhi ◽  
Mimin Aminah
Keyword(s):  
High Yield ◽  
Soybean Production ◽  
Alternative Crop ◽  
Use Of Technology ◽  
Self Sufficiency ◽  
Production And Consumption ◽  
Production And Distribution ◽  
Technology Applications ◽  
Cultivation Technology ◽  
Current Price

<strong>English</strong><br />This paper aims to describe the state of production and consumption of soybean, the use of technology by farmers, and the factors supporting self-sufficiency in soybean. The main problem of the soybean economy is the difficulty to boost production to meet the fast growing consumption.  Stagnation of soybean production is due to incompletely technology applications and the use of low quality seeds leaving a low level of productivity.  The current price of the locally produced soybean  is not able to compete with the imported soybean.  The factors supporting self-sufficiency are the invention of high yield seed variety and better cultivation technology which necessary to transfer to the farmers to achieve the same level of productivity as that in research level.  However, invention of high yield seed needs an improvement of seeds supply system, both in terms of production and distribution.  In this context, Indonesia has huge marginal land for extensification to increase soybean production, although technology engineering for this purpose is required.  The other important supporting factor is the import tariff application for soybean with bound rate of 27 percent, although this is not currently applicable. Indonesia has a potential crop to substitute soybean.  However, efforts to promote the consumption of this alternative crop substitution are required to gradually replace soybean in the Indonesian diet.<br /><br /><br /><strong>Indonesian</strong><br />Tulisan ini bertujuan untuk mendeskripsikan keadaan produksi dan konsumsi  komoditas kedelai, penggunaan teknologi oleh petani, serta faktor-faktor pendukung swasembada.  Permasalahan utama dalam ekonomi kedelai adalah sulitnya memacu produksi untuk memenuhi konsumsi yang meningkat dengan pesat.  Terhambatnya produksi kedelai disebabkan antara lain karena teknologi tidak digunakan sepenuhnya dan kurangnya penggunaan benih bermutu, yang menyebabkan produktivitasnya tetap rendah.  Dengan harga yang berlaku saat ini, kedelai produksi lokal tidak mampu bersaing dengan kedelai impor. Faktor-faktor yang mendukung swasembada adalah dihasilkannya varietas kedelai yang memiliki produktivitas tinggi dan teknologi budidaya yang lebih baik, untuk ditransfer ke petani agar dapat menyamai produktivitas di tingkat penelitian.  Namun demikian ditemukannya varietas unggul perlu didukung oleh perbaikan sistem penyediaan benih bermutu, baik dari sisi produksi maupun distribusinya.  Indonesia juga memiliki lahan yang cukup luas untuk meningkatkan produksi kedelai di lahan marjinal, walaupun memerlukan rekayasa teknologi.  Faktor berikutnya yang tidak kalah pentingnya dalam mendukung swasembada kedelai adalah penerapan bea masuk impor yang saat ini masih diperkenankan sampai batas 27 persen, namun saat ini tidak dimanfaatkan.  Selain itu, Indonesia juga memiliki komoditas substitusi yang sangat potensial untuk dikembangkan sebagai pengganti kedelai.  Diperlukan promosi diversifikasi substitusi kedelai agar secara bertahap komoditas alternatif tersebut dapat menggantikan kedelai dalam konsumsi rakyat Indonesia.

scholarly journals Erratum to: Anoxic metabolism and biochemical production in Pseudomonas putida F1 driven by a bioelectrochemical system

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Bin Lai ◽  
Shiqin Yu ◽  
Paul V. Bernhardt ◽  
Korneel Rabaey ◽  
Bernardino Virdis ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Bioelectrochemical System ◽  
Pseudomonas Putida F1 ◽  
Biochemical Production

scholarly journals PERSEPSI PETANI TERHADAP TEKNOLOGI “PANCA KELOLA” DI LAHAN RAWA BEKAS TERBAKAR (Kasus lahan gambut bekas terbakar Desa Talio Hulu Kecamatan Pandih Batu Kab. Pulang Pisau)

2021 ◽  
Vol 46 (3) ◽  
pp. 408
Author(s):  
Yanti Rina Darsani ◽  
Yiyi Sulaeman ◽  
Masganti Masganti
Keyword(s):  
Rice Production ◽  
Rice Cultivation ◽  
Yield Potential ◽  
Primary Data ◽  
High Yield ◽  
Survey Method ◽  
Urea Fertilizer ◽  
Land Preparation ◽  
Management Technology ◽  
Cultivation Technology

To understand the potential for increasing rice production on burnt peatlands, a demo farm (demonstration of grouped farming and fishing) which is a collaboration between Balittra and BRG which covers an area of 38 ha in Talio Hulu Village, Pandih Batu District, Pulang Pisau Regency, Central Kalimantan in 2020-2021. This study aims to discover the farmers' perceptions of the technology components and performance of rice cultivation technology. The activities have been carried out on farmers' land by applying “Five Management” technology include (1) water management techniques; (2) land preparation, (3) amelioration and fertilization (4) utilization of new high yielding varieties with high yield potential, and (5) integrated pest and disease control. Data were collected from 24 cooperating farmers by survey method. Primary data were collected by conducting direct interviews with farmers using a structured questionnaire. The data collected was tabulated and analyzed descriptively. The results showed that burned peatlands have the potential to increase rice production. The results showed that burned peatlands have the potential to increase rice production.. The response of farmers to the components of rice cultivation technology is positive. The five technology components that have the highest value with a value of 95.83% are (1) water regulation in paddy fields, (2) the utilization of ash and urea fertilizer in the nursery, (3) the utilization of manure and lime as ameliorant, (4) the utilization of urea fertilizer, SP-36, and NPK, and (5) the utilization of natural phosphate. The farmer's perception of the Five Management technology is positive.  Economically, rice farming of the Inpara 2 variety is efficient (R/C = 1.14). 

scholarly journals Indigenous Knowledge Cultivation of Local Rice Varieties “Siam Mutiara” and “Siam Saba” at Tidal Swampland

2020 ◽  
Vol 20 ◽  
pp. 01007
Author(s):  
Izhar Khairullah
Keyword(s):  
Indigenous Knowledge ◽  
Amylose Content ◽  
Paddy Fields ◽  
Yield Potential ◽  
High Yield ◽  
Rice Varieties ◽  
Local Varieties ◽  
Tidal Swamp ◽  
Cultivation Technology ◽  
High Yield Potential

Tidal swampland at present and in the future is very strategic as one of the national food barns considering sloping of productive land. In South Kalimantan, for example, tidal swamp paddy fields are available which is quite large, but the most are still planted with local varieties. More than 70% of rice cultivation in the tidal swampland is planted with various local varieties. The existence of local rice varieties is inseparable from its adaptability and acceptability factors. Among local varieties that are popular and widely planted by farmers are Siam Saba and Siam Mutiara varieties. Siam Saba and Siam Mutiara were released as local-improved varieties. Both of these varieties have their respective advantages, both from the distribution of plant adaptation and the potential benefits of yield. Siam Saba variety is widespread in tidal swampland of acid sulfate soil types and soils that are seawater intrusion in the dry season (near the coast) with A/B overflow type, while Siam Mutiara in acid sulfate tidal paddy fields with B / C overflow type. Some of the advantages of Siam Saba compared to other local varieties are high yield potential of 4.70 t / ha, a large number of tillers, small and slender grain shape, white rice color, and amylose content of 81.69%. Whereas improved of Siam Mutiara is high yield potential of 4.80 t / ha, the color of clean yellow grain, the color of clear and translucent rice such as pearl, and amylose content of 48.88%. Both varieties showed uniform in growth, flowering time, and ripening simultaneously, in mature panicles with almost no green grains, and a high percentage of unfertile grains. Local rice cultivation technology (Siam Saba and Siam Mutiara) indigenous knowledge here includes seedbed, transplanting, land preparation, fertilizing, pest disease control, harvest, and post-harvest processing or yields carried out by indigenous farmers. This indigenous cultivation technology until now in tidal swamp paddy fields of South Kalimantan is still widely practiced by farmers, especially for ownership of narrow or limited rice fields. There were advantages and disadvantages of each in the practice of indigenous cultivation technology.

scholarly journals Coupling an Electroactive Pseudomonas putida KT2440 with Bioelectrochemical Rhamnolipid Production

2020 ◽  
Vol 8 (12) ◽  
pp. 1959
Author(s):  
Theresia D. Askitosari ◽  
Carola Berger ◽  
Till Tiso ◽  
Falk Harnisch ◽  
Lars M. Blank ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Oxygen Demand ◽  
Extracellular Electron Transfer ◽  
Foam Formation ◽  
Proof Of Concept ◽  
Bioelectrochemical System ◽  
Oxygen Requirement ◽  
Pseudomonas Putida Kt2440 ◽  
Rhamnolipid Production ◽  
Major Bottleneck

Sufficient supply of oxygen is a major bottleneck in industrial biotechnological synthesis. One example is the heterologous production of rhamnolipids using Pseudomonas putida KT2440. Typically, the synthesis is accompanied by strong foam formation in the reactor vessel hampering the process. It is caused by the extensive bubbling needed to sustain the high respirative oxygen demand in the presence of the produced surfactants. One way to reduce the oxygen requirement is to enable the cells to use the anode of a bioelectrochemical system (BES) as an alternative sink for their metabolically derived electrons. We here used a P. putida KT2440 strain that interacts with the anode using mediated extracellular electron transfer via intrinsically produced phenazines, to perform heterologous rhamnolipid production under oxygen limitation. The strain P. putida RL-PCA successfully produced 30.4 ± 4.7 mg/L mono-rhamnolipids together with 11.2 ± 0.8 mg/L of phenazine-1-carboxylic acid (PCA) in 500-mL benchtop BES reactors and 30.5 ± 0.5 mg/L rhamnolipids accompanied by 25.7 ± 8.0 mg/L PCA in electrode containing standard 1-L bioreactors. Hence, this study marks a first proof of concept to produce glycolipid surfactants in oxygen-limited BES with an industrially relevant strain.

Enabling anoxic acetate assimilation by electrode-driven respiration in the obligate aerobe, Pseudomonas putida

2021 ◽  
Vol 138 ◽  
pp. 107690
Author(s):  
Sakuntala Mutyala ◽  
Changman Kim ◽  
Young Eun Song ◽  
Himanshu Khandelwal ◽  
Jiyun Baek ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Acetate Assimilation ◽  
Obligate Aerobe

scholarly journals Adoption of lentil varieties in Bangladesh: An expert elicitation approach

2018 ◽  
Vol 43 (1) ◽  
pp. 159-168
Author(s):  
MA Rashid ◽  
TMB Hossain ◽  
ME Hoque ◽  
MM Rahman ◽  
KS Rahman
Keyword(s):  
Food Security ◽  
Seed Production ◽  
Expert Elicitation ◽  
High Yield ◽  
Rust Disease ◽  
Nutrition Security ◽  
Cultivation Technology ◽  
Winter Crops ◽  
Elicitation Procedure ◽  
Information Database

The study was undertaken to find out variety wise adoption rate of lentil in Bangladesh through expert elicitation procedure. Many varieties have been developed by BARI and BINA but in details of varietal information and adoption information database was not developed which is very important and valuable for the scientist and policy planner. From all over the Bangladesh, 12 experts was invited to share their valuable knowledge and experience on lentil cultivation and adoption in the country. The average age of the experts were 51 yrs and average experience on lentil adoption was 21 yrs. The lentil expert informed that 16 major varieties are adopted by the farmers in the recent year (2013-14). Among those varieties, BARI Masur-6 covered highest cultivated area (54,642 ha) which shared 30.04% of total lentil cultivated area. BARI Masur-4, BARI Masur-3 and BARI Masur-5 ranked 2nd, 3rd and 4th position according to the share of cultivated area covered. The seed production information showed that BADC the only lentil seed producer supplied 2151 mt of lentil seed in the year 2009-2013.The adoption of variety BARI Masur-6 increased due to its high yield attribute. The another variety BARI Masur-4 and BARI Masur-3 adoption increased due to its high yield, resistant to rust disease attributes which showed increasing adoption path among the expert. Satisfying higher demand for lentil consumption and ensuring food security through providing alternative to winter crops are the major concerning issue of the policy planner and the scientist. To ensure nutrition security in the country, it is very important to encourage and support the farmers to grow more lentil through providing improved cultivation technology to the farmers.Bangladesh J. Agril. Res. 43(1): 159-168, March 2018

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