scholarly journals Two New Isoprenoid Flavonoids from Sophora flavescens with Antioxidant and Cytotoxic Activities

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7228
Author(s):  
Jingjing Li ◽  
Yan Lin ◽  
Lei He ◽  
Rongxiu Ou ◽  
Tao Chen ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chinese Medicine ◽  
Inhibitory Activity ◽  
Normal Cell ◽  
Cytotoxic Activities ◽  
Tumor Cell Lines ◽  
Sophora Flavescens ◽  
Normal Cell Line ◽  
Ic50 Values ◽  
New Compounds

Sophora flavescens is a regularly used traditional Chinese medicine. In an attempt to discover adequate active agents, the isoprenoid flavonoids from S. flavescens were further investigated. In this work, two new compounds (1–2, kurarinol A-B) together with 26 known ones (3–28) were isolated and elucidated on the basis of extensive NMR, UV and MS analyses. Furthermore, the antioxidant activity of all constituents was assessed through ABTS, PTIO and DPPH methodologies and also were evaluated for cytotoxic activity by three tumor cell lines (HepG2, A549 and MCF7) and one human normal cell line (LO2 cells). As a result, a multitude of components revealed significant inhibitory activity. In particular, compound 1–2 (kurarinol A-B), two new flavanonols derivatives, exhibited the most potent ABTS inhibitory activity with IC50 of 1.21 µg/mL and 1.81 µg/mL, respectively. Meanwhile, the new compound 1 demonstrated remarkable cytotoxicity against three cancer cells lines with IC50 values ranging from 7.50–10.55 μM but showed little effect on the normal cell. The two new isoprenoid flavonoids could be promising antioxidant and anti-tumor nature agents.

scholarly journals Antibacterial and Cytotoxic Phenolic Polyketides from Two Marine-Derived Fungal Strains of Aspergillus unguis

2022 ◽  
Vol 15 (1) ◽  
pp. 74
Author(s):  
Cao Van Anh ◽  
Joo-Hee Kwon ◽  
Jong Soon Kang ◽  
Hwa-Sun Lee ◽  
Chang-Su Heo ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Detailed Analysis ◽  
Cell Lines ◽  
Spectroscopic Data ◽  
Bacterial Activity ◽  
Tumor Cell Lines ◽  
Fungal Strains ◽  
Aspergillus Unguis ◽  
Ic50 Values ◽  
New Compounds

A chemical investigation on the EtOAc extracts from two marine-derived fungal strains of Aspergillus unguis resulted in the isolation of three previously undescribed phenolic polyketides including unguidepside C (1), aspersidone B (3), and agonodepside C (12), and their 14 known congeners. The structures of the new compounds were determined based on detailed analysis and comparison of their spectroscopic data with literature values, as well as Snatzke’s method. The new compounds (1, 3, and 12) displayed a significant anti-Gram-positive bacterial activity, with MIC values ranging from 5.3 to 22.1 µM. Additionally, the isolated compounds (1–11 and 13–16) were evaluated for their cytotoxicity against a panel of tumor cell lines. Most of them (except for 9) displayed cytotoxicity against all the tested cell lines, with IC50 values ranging from 2.5 to 46.9 µM.

scholarly journals Two New Cytotoxic Compounds from a Deep-Sea Penicillum citreonigrum XT20-134

Marine Drugs ◽  
2019 ◽  
Vol 17 (9) ◽  
pp. 509 ◽  
Author(s):  
Xi-Xiang Tang ◽  
Shun-Zhi Liu ◽  
Xia Yan ◽  
Bo-Wen Tang ◽  
Mei-Juan Fang ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Deep Sea ◽  
Cytotoxic Activities ◽  
Ionization Mass ◽  
Human Hepatoma ◽  
Etoac Extract ◽  
Deep Sea Sediment ◽  
Cytotoxic Compounds ◽  
Ic50 Values ◽  
New Compounds

Penicillum citreonigrum XT20-134 (MCCC 3A00956) is a fungus with cytotoxic activity, derived from deep-sea sediment. Five new compounds, adeninylpyrenocine (1), 2-hydroxyl-3-pyrenocine-thio propanoic acid (2), ozazino-cyclo-(2,3-dihydroxyl-trp-tyr) (3), 5,5-dichloro-1-(3,5-dimethoxyphenyl)-1,4-dihydroxypentan-2-one (4), and 2,3,4-trihydroxybutyl cinnamate (5), together with 19 known compounds (6–24), were isolated from an ethyl acetate (EtOAc) extract of its fermentation. The structures of the new compounds were comprehensively characterized by high-resolution electrospray ionization-mass spectrometry (HR-ESI-MS), 1D and 2D nuclear magnetic resonance (NMR). All isolates were evaluated for their cytotoxic activities. The heteroatom-containing new compounds 2 and 4 showed potent cytotoxicity to the human hepatoma tumor cell Bel7402 with IC50 values of 7.63 ± 1.46, 13.14 ± 1.41 μM and the human fibrosarcoma tumor cell HT1080 with IC50 values of 10.22 ± 1.32, 16.53 ± 1.67 μM, respectively.

scholarly journals Secondary metabolites of Phlebopus species from Northern Thailand

2020 ◽  
Vol 19 (12) ◽  
pp. 1525-1536
Author(s):  
Boontiya Chuankid ◽  
Hedda Schrey ◽  
Benjarong Thongbai ◽  
Olivier Raspé ◽  
Norbert Arnold ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
2D Nmr ◽  
Cytotoxic Activities ◽  
Tumor Cell Lines ◽  
Submerged Cultures ◽  
Esi Ms ◽  
Spectroscopic Analyses ◽  
New Compounds ◽  
Comprehensive Characterization

AbstractSubmerged cultures of the edible mushrooms Phlebopus portentosus and Phlebopus spongiosus were screened for their secondary metabolites by HPLC-UV/Vis and HR-LC-ESI-MS. Two new compounds, 9′-hydroxyphenyl pulvinone (1), containing an unusual pulvinone structure, and phlebopyron (2), together with the seven known pigments, atromentic acid (3), xerocomic acid (4), variegatic acid (5), methyl atromentate (6), methyl isoxerocomate (7), methyl variegatate (8), and variegatorubin (9) were isolated from the cultures. Their structures were assigned on the basis of extensive 1D/2D NMR spectroscopic analyses, as well as HR-ESI-MS, and HR-ESI-MS/MS measurements. Furthermore, the isolated compounds were evaluated for their antimicrobial and cytotoxic properties. 9′-hydroxyphenyl pulvinone (1), xerocomic acid (4), and methyl variegatate (8) exhibited weak to moderate cytotoxic activities against several tumor cell lines. The present paper provides a comprehensive characterization of pigments from the class of pulvinic acids that are present in the basidiomes of many edible bolete species.

scholarly journals Cytotoxic Polyketides from the Marine Sponge-Derived Fungus Pestalotiopsis heterocornis XWS03F09

Molecules ◽  
2019 ◽  
Vol 24 (14) ◽  
pp. 2655 ◽  
Author(s):  
Lei ◽  
Lei ◽  
Zhou ◽  
Hu ◽  
Niu ◽  
...  
Keyword(s):  
Marine Sponge ◽  
Spectroscopic Analysis ◽  
Fermentation Broth ◽  
Human Cancer ◽  
Optical Rotation ◽  
Cytotoxic Activities ◽  
Human Cancer Cell Lines ◽  
Chemical Structures ◽  
Ic50 Values ◽  
New Compounds

Four new compounds, including two new polyketides, heterocornols M and N (1, 2), and a pair of epimers, heterocornols O and P (3, 4), were isolated from the fermentation broth of the marine sponge-derived fungus Pestalotiopsis heterocornis XWS03F09, together with three known compounds (5–7). The new chemical structures were established on the basis of a spectroscopic analysis, optical rotation, experimental and calculated electronic circular dichroism (ECD). All of the compounds (1–7) were evaluated for their cytotoxic activities, and heterocornols M-P (1–4) exhibited cytotoxicities against four human cancer cell lines with IC50 values of 20.4–94.2 μM.

scholarly journals Bioactivity-Guided Purification of Novel Herbal Antioxidant and Anti-NO Compounds from Euonymus laxiflorus Champ.

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 120 ◽  
Author(s):  
Van Nguyen ◽  
San-Lang Wang ◽  
Anh Nguyen ◽  
Zhi-Hu Lin ◽  
Chien Doan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Natural Products ◽  
Biological Activities ◽  
The Novel ◽  
Health Food ◽  
Ic50 Values ◽  
Comparable Activity ◽  
New Compounds ◽  
First Time ◽  
Antidiabetic Effects

Euonymus laxiflorus Champ., a medicinal herb collected in Vietnam, has been reported to show several potent bioactivities, including anti-NO, enzyme inhibition, hypoglycemic and antidiabetic effects. Recently, the antioxidant activity of Euonymus laxiflorus Champ. trunk bark (ELCTB) has also been reported. However, the active antioxidant and anti-NO constituents existing in ELCTB have not been reported in the literature. The objective of this study was to purify the active antioxidants from ELCTB and investigate the anti-NO effect of the major constituents. Twenty-two phenolics isolated from ELCTB, including 12 compounds newly isolated in this study (1–12) and 10 constituents obtained from our previous work, were evaluated for their antioxidant activity. Of these, 12 compounds (4–6, 9, 13–15, 18–22) showed a potent antioxidant capacity (FRS50 = 7.8–58.11 µg/mL), in comparison to α-tocopherol (FRS50 = 23 µg/mL). In the anti-NO activity tests, Walterolactone A (1a) and B (1b) β-d-glucopyranoside (13) demonstrated the most effective and comparable activity to that of quercetin with max inhibition and IC50 values of 100%, 1.3 µg/mL, and 100%, 1.21 µg/mL, respectively. The crude extract and its major compounds showed no cytotoxicity on normal cells. Notably, three constituents (9, 11, and 12) were identified as new compounds, another three constituents, including 1, 7, and 8, were found to be new natural products, constituents 9 and 13 were determined to be new antioxidants, and compound 13 was reported to have novel potent anti-NO activity for the first time. The results of this study contribute to the enrichment of new natural products and compounds, as well as the novel biological activity of constituents isolated from Euonymus laxiflorus Champ. The current study also indicates ELCTB as a rich natural source of active phenolics. It is suggested that ELCTB could be developed as a health food with promising antioxidant and anti-NO effects, as well as other beneficial biological activities.

scholarly journals Dimeric 1,4-benzoquinone Derivatives with Cytotoxic Activities from the Marine-Derived Fungus Penicillium sp. L129

Marine Drugs ◽  
2019 ◽  
Vol 17 (7) ◽  
pp. 383 ◽  
Author(s):  
Zhang ◽  
Ju ◽  
Li ◽  
Sun ◽  
Peng ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Cell Lines ◽  
Inhibitory Activity ◽  
Spectroscopic Data ◽  
Cytotoxic Activities ◽  
Penicillium Sp ◽  
Ic50 Values ◽  
Cytotoxicity Activities ◽  
Mic Value ◽  
Mcf 7

Two new dimeric 1,4-benzoquinone derivatives, peniquinone A (1) and peniquinone B (2), a new dibenzofuran penizofuran A (3), and a new pyrazinoquinazoline derivative quinadoline D (4), together with 13 known compounds (5–17), were isolated from a marine-derived fungus Penicillium sp. L129. Their structures, including absolute configurations, were elucidated by extensive spectroscopic data and electronic circular dichroism calculations. Compound 1 exhibited cytotoxicity against the MCF-7, U87 and PC3 cell lines with IC50 values of 12.39 µM, 9.01 µM and 14.59 µM, respectively, while compound 2 displayed relatively weak cytotoxicity activities against MCF-7, U87 and PC3 cell lines with IC50 values of 25.32 µM, 13.45 µM and 19.93 µM, respectively. Furthermore, compound 2 showed weak quorum sensing inhibitory activity against Chromobacterium violaceum CV026 with an MIC value of 20 μg/well.

scholarly journals Tyrosinase Inhibitor and Antioxidant Activity of Seaweed Powder from Fresh and Dried Sargassum plagyophyllum

2018 ◽  
Vol 20 (3) ◽  
pp. 488 ◽  
Author(s):  
Ayun Erwina Arifianti ◽  
Effionora Anwar ◽  
Nurjanah Nurjanah
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Active Substance ◽  
Inhibitory Activity ◽  
Kojic Acid ◽  
Radical Scavenging ◽  
Tyrosinase Inhibitor ◽  
Optimal Result ◽  
Ic50 Values ◽  
Skin Lightening

<p>Sargassum plagyophyllum from Sargassaceae family contains various bioactive compounds, namely<br />phlorotannin which is reported as an antioxidant and tyrosinase inhibitor. Tyrosinase inhibitor and<br />antioxidant activity from seaweed powder that obtained from seaweed’s slurry have not been reported. Thus,<br />this study was aimed to obtain the best seaweed slurry and powder from S. plagyophyllum based on tyrosinase<br />inhibitor and antioxidant activity, so it can be used as active substance in skin lightening cosmetic formula.<br />S. plagyophyllum which prepared fresh and dried was processed into seaweed slurry and lyophilization<br />to form powder. Antioxidant activity which was determined by 2,2-Diphenyl-1-picrylhydrazyl (DPPH)<br />radical scavenging method found the IC50 values of ascorbic acid was 0.0035 mg/mL; fresh slurry 27.31<br />mg/mL; dried slurry 41.13 mg/mL; fresh powder 2.21 mg/mL; and dried powder 13.18 mg/mL. Moreover,<br />the tyrosinase inhibitory activity which was measured by enzimatic reaction with L-tyrosine as substrate<br />found IC50 values kojic acid 0.0076 mg/mL; fresh powder 4.97 mg/mL; and dried powder 11.35 mg/mL. Seaweed powder obtained from fresh ingredient is the most optimal result based on its tyrosinase inhibitor<br />and antioxidant activity, thus potential to be developed further as active substance for lightening cosmetic<br />formula.<br /><br /></p>

scholarly journals Synthesis and Biological Evaluation of 2-methyl-1Hbenzimidazole and 1H-benzimidazol-2-yl-methanol

2016 ◽  
Vol 15 (1) ◽  
pp. 83-87 ◽  
Author(s):  
Saikat Kumar Poddar ◽  
Nazmus Saqueeb ◽  
SM Abdur Rahman
Keyword(s):  
Antioxidant Activity ◽  
Biological Evaluation ◽  
Benzimidazole Derivatives ◽  
Cytotoxic Activities ◽  
Lc50 Value ◽  
Ic50 Value ◽  
Vincristine Sulphate ◽  
Ic50 Values ◽  
High Yields ◽  
Synthesis And Biological Evaluation

Two benzimidazole derivatives namely 2-methyl-1H-benzimidazole (1) and 1H-benzimidazol-2- ylmethanol (2) were synthesized in high yields by condensing O-phenylenediamine with two different carboxylic acid derivatives: glacial acetic acid and glycolic acid, following a different synthetic protocol. Structures were elucidated by spectroscopic methods. Among the two benzimidazole derivatives synthesized, 2-methyl-1Hbenzimidazole (1) showed moderate antioxidant activity with IC50 values of 144.84?g/ml whereas 1H-benzimidazol- 2-ylmethanol (2) displayed mild antioxidant activity (IC50 value 400.42 ?g/ml). Compound 1 also exhibited prominent cytotoxic activities with the LC50 value of 0.42 ?g/ml when compared to the standard vincristine sulphate (LC50 value of 0.544 ?g/ ml). It also demonstrated weak antimicrobial activity having 7-8 mm of zone of inhibition.Dhaka Univ. J. Pharm. Sci. 15(1): 83-87, 2016 (June)

scholarly journals Secondary Metabolites with α-Glucosidase Inhibitory Activity from the Mangrove Fungus Mycosphaerella sp. SYSU-DZG01

Marine Drugs ◽  
2019 ◽  
Vol 17 (8) ◽  
pp. 483 ◽  
Author(s):  
Pei Qiu ◽  
Zhaoming Liu ◽  
Yan Chen ◽  
Runlin Cai ◽  
Guangying Chen ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Single Crystal ◽  
Absolute Configuration ◽  
Inhibitory Activity ◽  
Spectroscopic Data ◽  
X Ray Diffraction ◽  
X Ray ◽  
Ic50 Values ◽  
New Metabolites

Four new metabolites, asperchalasine I (1), dibefurin B (2) and two epicoccine derivatives (3 and 4), together with seven known compounds (5–11) were isolated from a mangrove fungus Mycosphaerella sp. SYSU-DZG01. The structures of compounds 1–4 were established from extensive spectroscopic data and HRESIMS analysis. The absolute configuration of 1 was deduced by comparison of ECD data with that of a known structure. The stereostructures of 2–4 were further confirmed by single-crystal X-ray diffraction. Compounds 1, 8 and 9 exhibited significant α-glucosidase inhibitory activity with IC50 values of 17.1, 26.7 and 15.7 μM, respectively. Compounds 1, 4, 6 and 8 showed antioxidant activity by scavenging DPPH· with EC50 values ranging from 16.3 to 85.8 μM.

scholarly journals Bioactivities of essential oils from different parts of Spiranthera odoratissima (Rutaceae)

Rodriguésia ◽  
2020 ◽  
Vol 71 ◽  
Author(s):  
Fernando Duarte Cabral ◽  
Cassia Cristina Fernandes ◽  
Arthur Barcelos Ribeiro ◽  
Iara Squarisi Squarisi ◽  
Denise Crispim Tavares ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Lines ◽  
Normal Cell ◽  
Human Tumor ◽  
Tumor Cell Lines ◽  
Human Tumor Cell ◽  
Human Tumor Cell Lines ◽  
Ic50 Values ◽  
Mcf 7

Abstract This paper aims to investigate, for the first time, in vitro antitubercular, antileishmanial and antiproliferative activities of essential oils (EOs) from S. odoratissima leaves and flowers - grown in midwestern Brazil - against Mycobacterium tuberculosis, promastigote forms of Leishmania amazonensis and human tumor cell lines. Antimycobacterial activity of EOs was evaluated in terms of the minimal inhibitory concentration (MIC). EOs from leaves and flowers showed to be active antimicrobials against M. tuberculosis, since MIC values were 150 µg/mL and 162.5 µg/mL, respectively. Both EOs exhibited significant activity against promastigote forms of L. amazonensis; IC50 values (50% growth inhibition) were 14.36 ± 2.02 (EOs from leaves) and 19.89 ± 2.66 µg/mL (EOs from flowers). Antiproliferative activity in normal (GM07492A, lung fibroblasts) and tumor (MCF-7, HeLa and M059J) cell lines was performed by the XTT assay; results were expressed as IC50 (50% cell growth inhibition) and the selective index was calculated. IC50 values of EOs from leaves and flowers obtained in normal cell lines for were 502.97 ± 40.33 µg/mL and 370.60 ± 2.01 µg/mL, respectively. Antiproliferative activity was observed against human tumor cell lines, whose IC50 values were significantly lower than those obtained in normal cell lines of MCF-7 cells (367.57 ± 4.46 µg/mL-EOs from leaves and 357.70 ± 1.85 µg/mL-EOs from flowers) and M059J cells (492.53 ± 56.67 µg/mL-EOs from leaves and 324.90 ± 6.72 µg/mL-EOs from flowers), thus, indicating selectivity. These in vitro results showed that EOs from S. odoratissima may be an antimycobacterial, antiparasitic and antitumor agent.

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