scholarly journals Study of Antibacterial and Anticancer Properties of bioAgNPs Synthesized Using Streptomyces sp. PBD-311B and the Application of bioAgNP-CNC/Alg as an Antibacterial Hydrogel Film against P. aeruginosa USM-AR2 and MRSA

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6414
Author(s):  
Hemalatha Murugaiah ◽  
Chow Lun Teh ◽  
Kai Chew Loh ◽  
Ahmad Ramli Mohamad Yahya ◽  
Nur Asshifa Md Noh ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Spherical Shape ◽  
Ftir Analysis ◽  
Selective Toxicity ◽  
Streptomyces Sp ◽  
Anticancer Properties ◽  
Hydrogel Film ◽  
Vis Spectroscopy

Here, we report the extracellular biosynthesis of silver nanoparticles (AgNPs) and determination of their antibacterial and anticancer properties. We also explore the efficacy of bioAgNPs incorporated in cellulose nanocrystals (CNCs) and alginate (Alg) for the formation of an antibacterial hydrogel film. Streptomyces sp. PBD-311B was used for the biosynthesis of AgNPs. The synthesized bioAgNPs were characterized using UV-Vis spectroscopy, TEM, XRD, and FTIR analysis. Then, the bioAgNPs’ antibacterial and anticancer properties were determined using TEMA and cytotoxicity analysis. To form the antibacterial hydrogel film, bioAgNPs were mixed with a CNC and Alg solution and further characterized using FTIR analysis and a disc diffusion test. The average size of the synthesized bioAgNPs is around 69 ± 2 nm with a spherical shape. XRD analysis confirmed the formation of silver nanocrystals. FTIR analysis showed the presence of protein capping at the bioAgNP surface and could be attributed to the extracellular protein binding to bioAgNPs. The MIC value of bioAgNPs against P. aeruginosa USM-AR2 and MRSA was 6.25 mg/mL and 3.13 mg/mL, respectively. In addition, the bioAgNPs displayed cytotoxicity effects against cancer cells (DBTRG-0.5MG and MCF-7) and showed minimal effects against normal cells (SVG-p12 and MCF-10A), conferring selective toxicity. Interestingly, the bioAgNPs still exhibited inhibition activity when incorporated into CNC/Alg, which implies that the hydrogel film has antibacterial properties. It was also found that bioAgNP-CNC/Alg displayed a minimal or slow release of bioAgNPs owing to the intermolecular interaction and the hydrogel’s properties. Overall, bioAgNP-CNC/Alg is a promising antibacterial hydrogel film that showed inhibition against the pathogenic bacteria P. aeruginosa and MRSA and its application can be further evaluated for the inhibition of cancer cells. It showed benefits for surgical resection of a tumor to avoid post-operative wound infection and tumor recurrence at the surgical site.

scholarly journals Cytotoxicity Effects ofAmoora rohitukaandchittagongaon Breast and Pancreatic Cancer Cells

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Leo L. Chan ◽  
Sherine George ◽  
Irfan Ahmad ◽  
Saujanya L. Gosangari ◽  
Atiya Abbasi ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Alternative Medicine ◽  
Cancer Cells ◽  
Antibacterial Properties ◽  
Chemotherapeutic Agents ◽  
Normal Fibroblast ◽  
Label Free ◽  
Anticancer Properties ◽  
Pancreatic Cancer Cells ◽  
Mcf 7

Chemotherapeutic agents for cancer are highly toxic to healthy tissues and hence alternative medicine avenues are widely researched. Majority of the recent studies on alternative medicine suggested thatAmoora rohitukapossesses considerable antitumor and antibacterial properties. In this work,rohitukaandchittagonga, fractionated with petroleum ether, dichloromethane, and ethanol, were explored for their anticancer potential against two breast cancer (MCF-7 and HTB-126) and three pancreatic cancer (Panc-1, Mia-Paca2, and Capan1). The human foreskin fibroblast, Hs68, was also included. Cytotoxicity of each extract was analyzed using the MTT assay and label-free photonic crystal biosensor assay. A concentration series of each extract was performed on the six cell lines. For MCF-7 cancer cells, thechittagonga(Pet-Ether and CH2Cl2) androhituka(Pet-Ether) extracts induced cytotoxicity; thechittagonga(EtoAC) androhituka(MeOH) extracts did not induce cytotoxicity. For HTB126, Panc-1, Mia-Paca2, and Capan-1 cancer cells, only thechittagongaCH2Cl2extract showed a significant cytotoxic effect. The extracts were not cytotoxic to normal fibroblast Hs68 cells, which may be correlated to the specificity ofAmooraextracts in targeting cancerous cells. Based on these results, further examination of the potential anticancer propertiesAmooraspecies and the identification of the active ingredients of these extracts is warranted.

scholarly journals Study of antibacterial activity of Streptomyces sp. SS473 isolated from plants against ESBL-producing Escherichia coli

2021 ◽  
Vol 63 (9) ◽  
pp. 26-32
Author(s):  
Trung Hieu Nguyen ◽  
◽  
Phuoc Dat Nguyen ◽  
Thi Ngoc Quyen Nguyen ◽  
Le Truc Ha Tran ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Broad Spectrum ◽  
Bacterial Infections ◽  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Morphological Identification ◽  
Rrna Gene ◽  
Streptomyces Sp ◽  
E Coli ◽  
New Antibiotics

The broad spectrum β-lactamase-producing E. coli(ESBL) is a dangerous bacterial pathogen in humans due to its resistance to many antibiotics. This is especially serious in the context of a limited number of new antibiotics for treating bacterial infections. This leads to a global public health threat and places an urgent need for new antibiotics. In this study, the authors investigated the antibacterial properties of an actinomyces strain isolated from the plant Clinacanthus nutans against the ESBL-producing E. coli strains. These actinomyces strains were designated as SS473. Moreover, SS473 showed a broad spectrum of antibacterial activity on several clinically isolated pathogenic bacteria. Culture media have different effects on the antibacterial activity of SS473. In stability tests, the antibacterial activity of strain SS473 remained at a temperature up to 80oC but was lost at pH 3 and 13. By contrast, the antibacterial activity was not affected by UV and protease treatments. Based on the results of morphological identification with specific media for Streptomyces and molecular identification on 16S rRNA gene, strain SS473 was suggested to belong to the Streptomyces genus and was named Streptomycessp. SS473. The results in this study will pave the way for the following research on the identification of secondary metabolites having antibacterial activity and their biosynthetic pathways in Streptomyces sp. SS473 in the future

scholarly journals Endophytic Streptomyces laurentii Mediated Green Synthesis of Ag-NPs with Antibacterial and Anticancer Properties for Developing Functional Textile Fabric Properties

Antibiotics ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 641 ◽  
Author(s):  
Ahmed M. Eid ◽  
Amr Fouda ◽  
Gniewko Niedbała ◽  
Saad El-Din Hassan ◽  
Salem S. Salem ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Broad Spectrum ◽  
Textile Industry ◽  
Pathogenic Bacteria ◽  
Viral Infections ◽  
Cotton Fabrics ◽  
Ag Nps ◽  
X Ray ◽  
Anticancer Properties ◽  
Vis Spectroscopy

Improvement of the medical textile industry has received more attention recently, especially with widespread of microbial and viral infections. Medical textiles with new properties, such as bacterial pathogens self-cleaning, have been explored with nanotechnology. In this study, an endophytic actinomycetes strain of Streptomyces laurentii R-1 was isolated from the roots of the medicinal plant Achillea fragrantissima. This is used as a catalyst for the mediated biosynthesis of silver nanoparticles (Ag-NPs) for applications in the textile industry. The biosynthesized Ag-NPs were characterized using UV-vis spectroscopy, Fourier transform infrared (FT-IR), transmission electron microscopy (TEM), and X-ray Diffraction (XRD), which confirmed the successful formation of crystalline, spherical metal nanoparticles. The biosynthesized Ag-NPs exhibited broad-spectrum antibacterial activity. Our data elucidated that the biosynthesized Ag-NPs had a highly cytotoxic effect against the cancerous caco-2 cell line. The selected safe dose of Ag-NPs for loading on cotton fabrics was 100 ppm, regarding their antibacterial activity and safe cytotoxic efficacy. Interestingly, scanning electron microscope connected with energy dispersive X-ray spectroscopy (SEM-EDX) of loaded cotton fabrics demonstrated the smooth distribution of Ag-NPs on treated fabrics. The obtained results highlighted the broad-spectrum activity of nano-finished fabrics against pathogenic bacteria, even after 5 and 10 washing cycles. This study contributes a suitable guide for the performance of green synthesized NPs for utilization in different biotechnological sectors.

scholarly journals Optimization of silver nanoparticles synthesis by the green method using Streptomyces sp. SSUT88A and their antimicrobial activity against Pseudomonas aeruginosa

2021 ◽  
Vol 948 (1) ◽  
pp. 012085
Author(s):  
A Rosyidah ◽  
N Nantapong ◽  
N Chudapongse ◽  
O Weeranantanapan ◽  
W Limphirat
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Spherical Shape ◽  
Green Method ◽  
Several Variables ◽  
Vis Spectroscopy ◽  
Environmentally Safe

Abstract The green method has become an environmentally safe and valuable alternative to synthesizing silver nanoparticles (AgNPs). The AgNPs has been applied as antimicrobial agents, which their toxicity depends on several variables that generate different ability to inhibit pathogenic bacteria. Therefore, the optimization of AgNPs synthesis plays an important role in providing good antimicrobial activity. In this study, the synthesis of AgNPs was carried out with three different parameters: time of incubation, pH, and temperature to inhibit Pseudomonas aeruginosa growth using cell-free supernatant of Streptomyces sp. SSUT88A. The UV-Vis spectroscopy and antimicrobial activity were measured to obtain the optimum condition for each condition. The 74.12 nm in the spherical shape of AgNPs were optimized at 37°C, under pH 7 for five days. The synthesized AgNPs exhibited antimicrobial activity against P. aeruginosa 1287 and multidrug-resistance P. aeruginosa N90PS.

scholarly journals Microwave Mediated Fast Synthesis of Silver Nanoparticles and Investigation of Their Antibacterial Activities for Gram-Positive and Gram-Negative Microorganisms

Crystals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 666
Author(s):  
Faheem Ahmed ◽  
Suliman Yousef AlOmar ◽  
Fadwa Albalawi ◽  
Nishat Arshi ◽  
Sourabh Dwivedi ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Bacterial Infections ◽  
Antibacterial Properties ◽  
Spherical Shape ◽  
Ammonium Bromide ◽  
Face Centered Cubic ◽  
Gram Positive ◽  
Tem Analysis ◽  
Gram Negative ◽  
Vis Spectroscopy

Herein, a simple one-step microwave irradiation technique has been used to synthesize the silver nanoparticles using silver nitrate (AgNO3) solution and cetyltrimethyl ammonium bromide (CTAB) as a stabilizing agent. The crystals of as-prepared nanoparticles were studied using X-ray diffraction (XRD) and a selected area electron diffraction (SAED) pattern, confirming the single-phase face-centered cubic structure. The optical property measured using UV-Vis spectroscopy shows an absorption maximum at 420 nm, which also confirms the formation of silver nanoparticles. Transmission electron microscopy (TEM) analysis revealed that the silver nanoparticles have a spherical shape with an average diameter of ~6 nm. The antibacterial properties of silver nanoparticles were investigated using both Gram-positive and Gram-negative microorganisms, such as Staphylococcus aureus, Pseudomonas aeruginosa, andEscherichia coli. Klebsiella pneumoniae, and Candida albicans. Results showed a highest zone of inhibition of about 35 mm against P. aeruginosa as compared with E. coli (21 mm), S. aureus (30 mm), K. pneumonia (28 mm), and C. albicans (29 mm). These studies suggested that silver nanoparticles prepared by this fast and effective method might be developed as antibacterial agents against an extensive range of microorganisms to control and stop the spreading and persistence of bacterial infections.

A Novel Triazole Derivative Drug Presenting In Vitro and In Vivo Anticancer Properties

2018 ◽  
Vol 18 (17) ◽  
pp. 1483-1493
Author(s):  
Ricardo Imbroisi Filho ◽  
Daniel T.G. Gonzaga ◽  
Thainá M. Demaria ◽  
João G.B. Leandro ◽  
Dora C.S. Costa ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Hepatic Function ◽  
Anticancer Properties ◽  
Mcf 7

Background: Cancer is a major cause of death worldwide, despite many different drugs available to treat the disease. This high mortality rate is largely due to the complexity of the disease, which results from several genetic and epigenetic changes. Therefore, researchers are constantly searching for novel drugs that can target different and multiple aspects of cancer. Experimental: After a screening, we selected one novel molecule, out of ninety-four triazole derivatives, that strongly affects the viability and proliferation of the human breast cancer cell line MCF-7, with minimal effects on non-cancer cells. The drug, named DAN94, induced a dose-dependent decrease in MCF-7 cells viability, with an IC50 of 3.2 ± 0.2 µM. Additionally, DAN94 interfered with mitochondria metabolism promoting reactive oxygen species production, triggering apoptosis and arresting the cancer cells on G1/G0 phase of cell cycle, inhibiting cell proliferation. These effects are not observed when the drug was tested in the non-cancer cell line MCF10A. Using a mouse model with xenograft tumor implants, the drug preventing tumor growth presented no toxicity for the animal and without altering biochemical markers of hepatic function. Results and Conclusion: The novel drug DAN94 is selective for cancer cells, targeting the mitochondrial metabolism, which culminates in the cancer cell death. In the end, DAN94 has been shown to be a promising drug for controlling breast cancer with minimal undesirable effects.

scholarly journals Multi-Walled Carbon Nanotubes Decorated with Guanidinylated Dendritic Molecular Transporters: An Efficient Platform for the Selective Anticancer Activity of Doxorubicin

Pharmaceutics ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 858
Author(s):  
Kyriaki-Marina Lyra ◽  
Archontia Kaminari ◽  
Katerina N. Panagiotaki ◽  
Konstantinos Spyrou ◽  
Sergios Papageorgiou ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Anticancer Activity ◽  
Cancer Cells ◽  
Delivery System ◽  
Colloidal Stability ◽  
Triggered Release ◽  
Selective Toxicity ◽  
Molecular Transporters ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

An efficient doxorubicin (DOX) drug delivery system with specificity against tumor cells was developed, based on multi-walled carbon nanotubes (MWCNTs) functionalized with guanidinylated dendritic molecular transporters. Acid-treated MWCNTs (oxCNTs) interacted both electrostatically and through hydrogen bonding and van der Waals attraction forces with guanidinylated derivatives of 5000 and 25,000 Da molecular weight hyperbranched polyethyleneimine (GPEI5K and GPEI25K). Chemical characterization of these GPEI-functionalized oxCNTs revealed successful decoration with GPEIs all over the oxCNTs sidewalls, which, due to the presence of guanidinium groups, gave them aqueous compatibility and, thus, exceptional colloidal stability. These GPEI-functionalized CNTs were subsequently loaded with DOX for selective anticancer activity, yielding systems of high DOX loading, up to 99.5% encapsulation efficiency, while the DOX-loaded systems exhibited pH-triggered release and higher therapeutic efficacy compared to that of free DOX. Most importantly, the oxCNTs@GPEI5K-DOX system caused high and selective toxicity against cancer cells in a non-apoptotic, fast and catastrophic manner that cancer cells cannot recover from. Therefore, the oxCNTs@GPEI5K nanocarrier was found to be a potent and efficient nanoscale DOX delivery system, exhibiting high selectivity against cancerous cells, thus constituting a promising candidate for cancer therapy.

scholarly journals Cholesterol Levels Affect the Performance of AuNPs-Decorated Thermo-Sensitive Liposomes as Nanocarriers for Controlled Doxorubicin Delivery

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 973
Author(s):  
Mónica C. García ◽  
Nabila Naitlho ◽  
José Manuel Calderón-Montaño ◽  
Estrella Drago ◽  
Manuela Rueda ◽  
...  
Keyword(s):  
Drug Release ◽  
Cancer Cells ◽  
Electrostatic Interactions ◽  
Drug Loading ◽  
Gradient Methods ◽  
Spherical Shape ◽  
Morphological Properties ◽  
Ovarian Cancer Cells ◽  
Cholesterol Levels ◽  
L1 And L2

Stimulus-responsive liposomes (L) for triggering drug release to the target site are particularly useful in cancer therapy. This research was focused on the evaluation of the effects of cholesterol levels in the performance of gold nanoparticles (AuNPs)-functionalized L for controlled doxorubicin (D) delivery. Their interfacial and morphological properties, drug release behavior against temperature changes and cytotoxic activity against breast and ovarian cancer cells were studied. Langmuir isotherms were performed to identify the most stable combination of lipid components. Two mole fractions of cholesterol (3.35 mol% and 40 mol%, L1 and L2 series, respectively) were evaluated. Thin-film hydration and transmembrane pH-gradient methods were used for preparing the L and for D loading, respectively. The cationic surface of L allowed the anchoring of negatively charged AuNPs by electrostatic interactions, even inducing a shift in the zeta potential of the L2 series. L exhibited nanometric sizes and spherical shape. The higher the proportion of cholesterol, the higher the drug loading. D was released in a controlled manner by diffusion-controlled mechanisms, and the proportions of cholesterol and temperature of release media influenced its release profiles. D-encapsulated L preserved its antiproliferative activity against cancer cells. The developed liposomal formulations exhibit promising properties for cancer treatment and potential for hyperthermia therapy.

scholarly journals One-step preparation of antimicrobial silicone materials based on PDMS and salicylic acid: insights from spatially and temporally resolved techniques

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Luca Barbieri ◽  
Ioritz Sorzabal Bellido ◽  
Alison J. Beckett ◽  
Ian A. Prior ◽  
Jo Fothergill ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Laser Scanning ◽  
Pathogenic Bacteria ◽  
Pharmaceutical Products ◽  
Laser Scanning Microscopy ◽  
Wound Dressings ◽  
Confocal Laser ◽  
Vis Spectroscopy ◽  
Wide Range ◽  
One Step

AbstractIn this work, we introduce a one-step strategy that is suitable for continuous flow manufacturing of antimicrobial PDMS materials. The process is based on the intrinsic capacity of PDMS to react to certain organic solvents, which enables the incorporation of antimicrobial actives such as salicylic acid (SA), which has been approved for use in humans within pharmaceutical products. By combining different spectroscopic and imaging techniques, we show that the surface properties of PDMS remain unaffected while high doses of the SA are loaded inside the PDMS matrix. The SA can be subsequently released under physiological conditions, delivering a strong antibacterial activity. Furthermore, encapsulation of SA inside the PDMS matrix ensured a diffusion-controlled release that was tracked by spatially resolved Raman spectroscopy, Attenuated Total Reflectance IR (ATR-IR), and UV-Vis spectroscopy. The biological activity of the new material was evaluated directly at the surface and in the planktonic state against model pathogenic bacteria, combining confocal laser scanning microscopy, electron microscopy, and cell viability assays. The results showed complete planktonic inhibition for clinically relevant strains of Staphylococcus aureus and Escherichia coli, and a reduction of up to 4 orders of magnitude for viable sessile cells, demonstrating the efficacy of these surfaces in preventing the initial stages of biofilm formation. Our approach adds a new option to existing strategies for the antimicrobial functionalisation of a wide range of products such as catheters, wound dressings and in-dwelling medical devices based on PDMS.

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