scholarly journals Characterization and Identification of Bioactive Polyphenols in the Trapabispinosa Roxb. Pericarp Extract

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5802
Author(s):  
Yuji Iwaoka ◽  
Shoichi Suzuki ◽  
Nana Kato ◽  
Chisa Hayakawa ◽  
Satoko Kawabe ◽  
...  
Keyword(s):  
Gallic Acid ◽  
High Performance ◽  
Positive Control ◽  
Mass Spectrometry Analysis ◽  
Ionization Mass ◽  
Inhibitory Effects ◽  
Spectrometry Analysis ◽  
Isolation And Characterization ◽  
Potential Food

In this study, we present the isolation and characterization of the structure of six gallotannins (1–6), three ellagitannins (7–9), a neolignan glucoside (10), and three related polyphenolic compounds (gallic acid, 11 and 12) from Trapa bispinosa Roxb. pericarp extract (TBE). Among the isolates, the structure of compound 10 possessing a previously unclear absolute configuration was unambiguously determined through nuclear magnetic resonance and circular dichroism analyses. The α-glucosidase activity and glycation inhibitory effects of the isolates were evaluated. Decarboxylated rugosin A (8) showed an α-glucosidase inhibitory activity, while hydrolyzable tannins revealed stronger antiglycation activity than that of the positive control. Furthermore, the identification and quantification of the TBE polyphenols were investigated by high-performance liquid chromatography coupled to ultraviolet detection and electrospray ionization mass spectrometry analysis, indicating the predominance of gallic acid, ellagic acid, and galloyl glucoses showing marked antiglycation properties. These findings suggest that there is a potential food industry application of polyphenols in TBE as a functional food with antidiabetic and antiglycation activities.

scholarly journals OPTIMIZATION, ISOLATION AND CHARACTERIZATION OF BIOACTIVE COMPOUNDS FROM STREPTOMYCES LAVENDULOCOLOR VHB-9

2018 ◽  
Vol 11 (8) ◽  
pp. 361
Author(s):  
Hima Bindu Bssn ◽  
Rajesh Kumar Munaganti ◽  
Vijayalakshmi Muvva ◽  
Krishna Naragani ◽  
Mani Deepa Indupalli
Keyword(s):  
Bioactive Compounds ◽  
Culture Conditions ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Ionization Mass ◽  
Isolation And Identification ◽  
Spectrometry Analysis ◽  
Isolation And Characterization ◽  
Bacteria And Fungi

Objectives: Optimization, isolation, and characterization of bioactive compounds from Streptomyces lavendulocolor VHB-9 isolated from granite mines of Mudigonda village of Khammam district of Telangana state.Methods: The potent strain was identified as S. lavendulocolor VHB-9 by polyphasic taxonomy. The influence of culture conditions on growth and bioactive compounds production was investigated. Purification of bioactive compounds was done using column chromatography. The structures of the compounds were elucidated on the basis of spectroscopic analysis including Fourier transform infrared, electron spray ionization mass spectrophotometry,1H nuclear magnetic resonance (NMR), and13C NMR. The antimicrobial activity of the compounds produced by the strain was tested against both Gram-positive and Gram-negative bacteria and fungi in terms of minimum inhibitory concentration.Results: Isolation and identification of two compounds, namely (2R, 3R)-2, 3-Butanediol (B1A), and nonadecanoic acid (B1B). Fraction B4 was isolated partially purified fraction and identified by the gas chromatography-mass spectrometry analysis. B1B compound exhibited the highest activity against Bacillus megaterium, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans when compared to B1A and B4 compounds.

scholarly journals Characterization of Low-Strigolactone Germplasm in Pea (Pisum sativum L.) Resistant to Crenate Broomrape (Orobanche crenata Forsk.)

2016 ◽  
Vol 29 (10) ◽  
pp. 743-749 ◽  
Author(s):  
Stefano Pavan ◽  
Adalgisa Schiavulli ◽  
Angelo Raffaele Marcotrigiano ◽  
Nicoletta Bardaro ◽  
Valentina Bracuto ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
High Performance ◽  
Resistance Mechanism ◽  
Mass Spectrometry Analysis ◽  
Orobanche Crenata ◽  
Breeding Programs ◽  
Spectrometry Analysis ◽  
Pisum Sativum L

Crenate broomrape (Orobanche crenata Forsk.) is a devastating parasitic weed threatening the cultivation of legumes around the Mediterranean and in the Middle East. So far, only moderate levels of resistance were reported to occur in pea (Pisum sativum L.) natural germplasm, and most commercial cultivars are prone to severe infestation. Here, we describe the selection of a pea line highly resistant to O. crenata, following the screening of local genetic resources. Time series observations show that delayed emergence of the parasite is an important parameter associated with broomrape resistance. High performance liquid chromatography connected to tandem mass spectrometry analysis and in vitro broomrape germination bioassays suggest that the resistance mechanism might involve the reduced secretion of strigolactones, plant hormones exuded by roots and acting as signaling molecules for the germination of parasitic weeds. Two years of replicated trials in noninfested fields indicate that the resistance is devoid of pleiotropic effects on yield, in contrast to pea experimental mutants impaired in strigolactone biosynthesis and, thus, is suitable for use in breeding programs.

scholarly journals Structural Characterization of an Oligosaccharide Made by Neisseria sicca

2009 ◽  
Vol 191 (10) ◽  
pp. 3311-3320 ◽  
Author(s):  
Ellen T. O'Connor ◽  
Hui Zhou ◽  
Kevin Bullock ◽  
Karen V. Swanson ◽  
J. McLeod Griffiss ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Structural Characterization ◽  
Lectin Binding ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Mass Spectrometry Analysis ◽  
Ionization Mass ◽  
Spectrometry Analysis ◽  
Neisseria Sicca

ABSTRACT Neisseria sicca 4320 expresses two carbohydrate-containing components with sodium dodecyl sulfate-polyacrylamide gel electrophoresis mobilities that resemble those of lipooligosaccharide and lipopolysaccharide. Using matrix-assisted laser desorption ionization—time of flight and electrospray ionization mass spectrometry, we characterized a disaccharide carbohydrate repeating unit expressed by this strain. Gas chromatography identified the sugars composing the unit as rhamnose and N-acetyl-d-glucosamine. Glycosidase digestion confirmed the identity of the nonreducing terminal sugar of the disaccharide and established its β-anomeric configuration. Mass spectrometry analysis and lectin binding were used to verify the linkages within the disaccharide repeat. The results revealed that the disaccharide repeat is [-4) β-l-rhamnose (1-3) β-N-acetyl-d-glucosamine (1-] with an N-acetyl-d-glucosamine nonreducing terminus. This work is the first structural characterization of a molecule that possesses rhamnose in the genus Neisseria.

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