scholarly journals Fluorescent Phthalocyanine-Encapsulated Bovine Serum Albumin Nanoparticles: Their Deployment as Therapeutic Agents in the NIR Region

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4679
Author(s):  
Raluca Borlan ◽  
Daria Stoia ◽  
Luiza Gaina ◽  
Andreea Campu ◽  
Gabriel Marc ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Biomedical Applications ◽  
Therapeutic Agents ◽  
Hydrodynamic Diameter ◽  
Fluorescent Nanoparticles ◽  
Docking Simulations ◽  
Viability Assay ◽  
Conversion Performance

In recent times, researchers have aimed for new strategies to combat cancer by the implementation of nanotechnologies in biomedical applications. This work focuses on developing protein-based nanoparticles loaded with a newly synthesized NIR emitting and absorbing phthalocyanine dye, with photodynamic and photothermal properties. More precisely, we synthesized highly reproducible bovine serum albumin-based nanoparticles (75% particle yield) through a two-step protocol and successfully encapsulated the NIR active photosensitizer agent, achieving a good loading efficiency of 91%. Making use of molecular docking simulations, we confirm that the NIR photosensitizer is well protected within the nanoparticles, docked in site I of the albumin molecule. Encouraging results were obtained for our nanoparticles towards biomedical use, thanks to their negatively charged surface (−13.6 ± 0.5 mV) and hydrodynamic diameter (25.06 ± 0.62 nm), favorable for benefitting from the enhanced permeability and retention effect; moreover, the MTT viability assay upholds the good biocompatibility of our NIR active nanoparticles. Finally, upon irradiation with an NIR 785 nm laser, the dual phototherapeutic effect of our NIR fluorescent nanoparticles was highlighted by their excellent light-to-heat conversion performance (photothermal conversion efficiency 20%) and good photothermal and size stability, supporting their further implementation as fluorescent therapeutic agents in biomedical applications.

scholarly journals Elucidation of the Interaction between Flavan-3-ols and Bovine Serum Albumin and Its Effect on Their In-Vitro Cytotoxicity

Molecules ◽  
2019 ◽  
Vol 24 (20) ◽  
pp. 3667
Author(s):  
Yasuyuki Fujii ◽  
Yoshitomo Suhara ◽  
Yusuke Sukikara ◽  
Tomohiro Teshima ◽  
Yoshihisa Hirota ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
In Vitro Cytotoxicity ◽  
Binding Energies ◽  
Docking Simulations ◽  
Fetal Bovine

Flavan-3-ols (FLs), specifically catechin and its oligomer B-type procyanidins, are suggested to potently bind to bovine serum albumin (BSA). We examined the interaction between BSA and FLs by fluorescence quenching and found the following order of binding activities to BSA: cinnamtannin A2 (A2; tetramer) > procyanidin C1 (C1; trimer) ≈ procyanidin B2 (B2, dimer) > (−)epicatechin (EC, monomer). Docking simulations between BSA and each compound at the binding site showed that the calculated binding energies were consistent with the results of our experimental assay. FLs exerted cytotoxicity at 1000 μg/mL in F11 cell culture with fetal bovine serum containing BSA. In culture containing serum-free medium, FLs exhibited significant cell proliferation at 10−4 μg/mL and cytotoxicity was observed at concentrations greater than 10 μg/mL. Results of this study suggest that interactions between polyphenols and BSA should be taken into account when evaluating procyanidin in an in vitro cell culture system.

Spectroscopy and docking simulations of the interaction between lochnericine and bovine serum albumin

Luminescence ◽  
2014 ◽  
Vol 30 (2) ◽  
pp. 240-246 ◽  
Author(s):  
Qing Wang ◽  
Jiawei He ◽  
Jin Yan ◽  
Di Wu ◽  
Hui Li
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Docking Simulations

scholarly journals Copper-containing nanosystems based on macromolecular hydrophilic stabilizers

2019 ◽  
Vol 489 (3) ◽  
pp. 254-257
Author(s):  
S. V. Valueva ◽  
O. V. Nazarova ◽  
M. E. Vylegzhanina ◽  
L. N. Borovikova ◽  
Yu. I. Zolotova ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Aqueous Solutions ◽  
Hydrazine Hydrate ◽  
Copper Nanoparticles ◽  
Bovine Serum ◽  
Biomedical Applications ◽  
Intermediate Stage ◽  
Original Method ◽  
Complex Ion

An original method for the synthesis of copper-containing nanosystems for biomedical applications using hydrazine hydrate as a reducing agent, intermediate stage of formation of complex ion [Cu(NH3)4]2+, which allows to obtain Cu0 nanoparticles without impurities of oxides has been developed. As a macromolecular hydrophilic stabilizer copolymer of 2-deoxy‑2-methacrylamido-D‑glucose with 2-dimethylaminoethylmethaacrylate or bovine serum albumin were used. It has been shown that such stabilization allows to achieve almost unimodal distribution of copper nanoparticles in aqueous solutions due to their rather good shielding by macromolecules.

Systematic spectroscopic investigation of structural changes and corona formation of bovine serum albumin over magneto-fluorescent nanoparticles

2020 ◽  
Vol 49 (35) ◽  
pp. 12380-12389 ◽  
Author(s):  
Ashish Tiwari ◽  
Prachi Bhatia ◽  
Jaspreet Kaur Randhawa
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Spectroscopic Investigation ◽  
Bovine Serum ◽  
Structural Changes ◽  
Fluorescent Nanoparticles ◽  
Specific Interactions ◽  
Corona Formation

Specific interactions between bovine serum albumin (BSA) and magneto-fluorescent nanoparticles (MFNPs).

scholarly journals Bovine Serum Albumin-Immobilized Black Phosphorus-Based γ-Fe2O3 Nanocomposites: A Promising Biocompatible Nanoplatform

Biomedicines ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 858
Author(s):  
Atanu Naskar ◽  
Sohee Lee ◽  
Dongjoon Ko ◽  
Semi Kim ◽  
Kwang-sun Kim
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Photoelectron Spectroscopy ◽  
Bovine Serum ◽  
Biomedical Applications ◽  
Physical Interaction ◽  
High Sequence Identity ◽  
X Ray ◽  
Interactive Behavior

The interactions between proteins and nanoparticles need to be fully characterized as the immobilization of proteins onto various nanoplatforms in the physiological system often results in the change of surface of the protein molecules to avoid any detrimental issues related to their biomedical applications. Hence, in this article, the successful low-temperature synthesis of a BP-based γ-Fe2O3 (IB) nanocomposite and its interactive behavior with bovine serum albumin (BSA)—a molecule with chemical similarity and high sequence identity to human serum albumin—are described. To confirm the formation of γ-Fe2O3 and the IB nanocomposite, X-ray diffraction, transmission electron microscopy, and X-ray photoelectron spectroscopy analyses of the materials were performed. Additionally, the physical interaction between BSA and the IB nanocomposite was confirmed via UV–Vis and photoluminescence spectral analyses. Finally, the biocompatibility of the BSA-immobilized IB nanocomposite was verified using an in vitro cytotoxicity assay with HCT-15 colon cancer cells. Our findings demonstrate that this newly developed nanocomposite has potential utility as a biocompatible nanoplatform for various biomedical applications.

Alternating Current Electrophoretic Deposition of Bovine Serum Albumin onto Magnesium

2015 ◽  
Vol 654 ◽  
pp. 139-143 ◽  
Author(s):  
Bram Neirinck ◽  
Ferdinand Singer ◽  
Annabel Braem ◽  
Sannakaisa Virtanen ◽  
Jef Vleugels
Keyword(s):  
Corrosion Resistance ◽  
Side Effects ◽  
Bovine Serum Albumin ◽  
Corrosion Rate ◽  
Serum Albumin ◽  
Magnesium Alloys ◽  
Electrophoretic Deposition ◽  
Bovine Serum ◽  
Biomedical Applications ◽  
Biodegradable Coatings

Magnesium and magnesium alloys are gaining considerable attention for use in biomedical applications due to their capability to completely resorb in the human body without noticeable side effects. For structural biomedical applications however, the resorption rate is too large. In order to decrease this rate researchers are investigating magnesium alloys with an increased corrosion resistance and/or biodegradable coatings, such as dense protein layers, which retard the resorption.In this work, we demonstrate the electrophoretic deposition of Bovine Serum Albumin (BSA) directly onto pure magnesium substrates using unbalanced alternating fields (AC-EPD). The effect of the obtained coatings on the corrosion behavior of the substrates was evaluated by potentiodynamic polarization. The results show that an albumin layer deposited by AC-EPD from a 50/50 ethanol/H2O medium significantly reduces the corrosion rate.

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