scholarly journals Behavioral and Antennal Responses of Tribolium confusum to Varronia globosa Essential Oil and Its Main Constituents: Perspective for Their Use as Repellent

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4393
Author(s):  
Cesar Auguste Badji ◽  
Jean Dorland ◽  
Lynda Kheloul ◽  
Dimitri Bréard ◽  
Pascal Richomme ◽  
...  

Essential oils of aromatic plants represent an alternative to classical pest control with synthetic chemicals. They are especially promising for the alternative control of stored product pest insects. Here, we tested behavioral and electrophysiological responses of the stored product pest Tribolium confusum, to the essential oil of a Brazilian indigenous plant, Varronia globosa, collected in the Caatinga ecosystem. We analyzed the essential oil by GC-MS, tested the effects of the entire oil and its major components on the behavior of individual beetles in a four-way olfactometer, and investigated responses to these stimuli in electroantennogram recordings (EAG). We could identify 25 constituents in the essential oil of V. globosa, with anethole, caryophyllene and spathulenole as main components. The oil and its main component anethole had repellent effects already at low doses, whereas caryophyllene had only a repellent effect at a high dose. In addition, the essential oil abolished the attractive effect of the T. confusum aggregation pheromone. EAG recordings revealed dose-dependent responses to the individual components and increasing responses to the blend and even more to the entire oil. Our study reveals the potential of anethole and the essential oil of V. globosa in the management of stored product pests.

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5693 ◽  
Author(s):  
Min Liao ◽  
Qian-Qian Yang ◽  
Jin-Jing Xiao ◽  
Yong Huang ◽  
Li-Jun Zhou ◽  
...  

Background In our previous study, Melaleuca alternifolia essential oil (EO) was considered to have an insecticidal effect by acting on the mitochondrial respiratory chain in insects. However, the mode of action is not fully understood. Methods In this study, we investigated the insecticidal efficacy of the M. alternifolia EO against another major stored-product pest, Tribolium confusum Jacquelin du Val. Rarefaction and vacuolization of the mitochondrial matrix were evident in oil-fumigated T. confusum adults. Results Alterations to the mitochondria confirmed the insecticidal effect of the M. alternifolia EO. Furthermore, comparative transcriptome analysis of T. confusum using RNA-seq indicated that most of the differentially expressed genes were involved in insecticide detoxification and mitochondrial function. The biochemical analysis showed that the intracellular NAD+/NADH ratio is involved in the differential effect of the M. alternifolia EO. Discussion These results led us to conclude that NAD+/NADH dehydrogenase may be the prime target site for the M. alternifolia EO in insects, leading to blocking of the mitochondrial respiratory chain.


Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 869 ◽  
Author(s):  
Călin Jianu ◽  
Ionuț Goleț ◽  
Daniela Stoin ◽  
Ileana Cocan ◽  
Alexandra Teodora Lukinich-Gruia

In the last decade, there has been growing interest in the food industry in replacing synthetic chemicals with natural products with bioactive properties. This study’s aims were to determine the chemical composition and the antioxidant properties of the essential oil of Pastianica sylvestris. The essential oil was isolated with a yield of 0.41% (w/v) by steam distillation from the dried seeds and subsequently analysed by GC-MS. Octyl acetate (78.49%) and octyl hexanoate (6.68%) were the main components. The essential oil exhibited an excellent activity for the inhibition of primary and secondary oxidation products for cold-pressed sunflower oil comparable with butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT), which were evaluated using peroxide and thiobarbituric acid values. The antioxidant activity of the essential oil was additionally validated using DPPH radical scavenging (0.0016 ± 0.0885 mg/mL), and β-carotene-linoleic acid bleaching assays. Also, the amounts of total phenol components (0.0053 ± 0.0023 mg GAE/g) were determined.


Plants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 448 ◽  
Author(s):  
Sandra Espinosa ◽  
Nicole Bec ◽  
Christian Larroque ◽  
Jorge Ramírez ◽  
Barbara Sgorbini ◽  
...  

The fresh leaves of Coreopsis triloba S.F. Blake, collected at Cerro Villonaco in Loja, Ecuador, were investigated with respect to their essential oil (EO). The chemical composition was determined qualitatively through gas chromatography coupled with mass spectrometry (GC-MS) and quantitatively by gas chromatography coupled with flame ionization (GC-FID), using relative response factors (RRF) based on the enthalpy of combustion. The essential oil contained between 92.5% and 93.4% of monoterpene hydrocarbons, with (E)-β-ocimene being the main component (35.2–35.9%), followed by β-phellandrene (24.6–25.0%), α-pinene (15.3–15.9%), myrcene (10.9–11.0%), sabinene (2.2–2.4%), (Z)-β-ocimene (1.5%), and germacrene D (1.2–1.3%). The enantiomeric distribution of α-pinene, β-pinene, limonene, and germacrene D was also determined. The main components responsible for the aroma were identified through aroma extract dilution analysis (AEDA), a gas chromatography-olfactometry (GC-O) based technique, being α-pinene, β-pinene (0.6%), terpinolene (0.1%), α-copaene (0.1–0.3%), β-phellandrene, and (E)-4,8-dimethyl-1,3,7-nonatriene (0.1–0.2%) the main olfactory constituents according to the decreasing factor of dilution (FD) order. The biological tests showed IC50 inhibition values of 42.2 and 6.8 µg/mL for acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), respectively.


2019 ◽  
pp. 58-62 ◽  
Author(s):  
N. N. Bakova ◽  
O. M. Shevchuk ◽  
L. A. Logvinenko ◽  
L. A. Timasheva

Tarragon (Artemisia dracunculus L.) is a spicy aromatic culture, perspective to use in the food and medical industry. In the collection of aromatic and medicinal plants of the Nikita Botanical Gardens, tarragon is represented by two varieties: Isumrud and Travnevy. The Isumrud variety is characterized by a high content of essential oil (0.6% of the wet weight) of the methylchavicol chemotype (the main component of the essential oil is methylchavicol, whose mass fraction is up to 91%) and is perspective to use not only as a spice, but also in medicine as a reducing facilities. The variety Travnevy contains only 0.1% of the essential oil from the wet mass of the sabineno-elemicin chemotype (the main components of the essential oil are sabinen (40.0%) and trans-from-iso-elemicin – 26.1%), characterized by high productivity of the aerial mass (12 kg/m2), the main direction of use in the food industry as a spice. Due to the lack of requirements for dry raw tarragon, we have developed a draft technical specifications for dry crushed tarragon raw materials TS 01.28.30-009-01579640-2018. Specifications apply to dried whole and crushed raw materials, intended for the manufacture of water and water-alcohol infusions, as a source of biologically active substances, as a component for seasonings to meat, fish dishes, for use in cooking, as well as in the production of alcohol and soft drinks. The main physicochemical indicators of whole and crushed tarragon raw materials (type, size, smell, taste, mass fraction of essential oil, impurities, moisture, ash) are presented.


2004 ◽  
Vol 129 (6) ◽  
pp. 789-794 ◽  
Author(s):  
Kelly M. Bowes ◽  
Valtcho D. Zheljazkov

Field and laboratory experiments were conducted during the summers of 2001 and 2002 in two locations in Nova Scotia to identify the effect of cultivar, transplanting date, and drying (air-drying and freeze-drying) on basil (Ocimum basilicum `Mesten' and `Italian Broadleaf', and O. sanctum `Local') productivity and oil quality in Nova Scotia and to identify the potential of growing basil as a cash crop in this region. Results suggested that all of the tested cultivars of basil grown in Nova Scotia had acceptable yields and composition for the international commercial market. Greater yields (ranging from 3.6 to 19.8 t·ha-1) were achieved from `Mesten' and `Italian Broadleaf' by earlier transplanting. `Local' had a lower oil content compared to the other cultivars. Linalool was the main component of `Mesten' oil, linalool and methyl chavicol were the main components of `Italian Broadleaf' oil, while elemene and α-humulene were the main components of `Local' oil. Both air-drying and freeze-drying were found to alter the composition of the essential oil from O. sanctum and O. basilicum.


2019 ◽  
Vol 9 (6) ◽  
pp. 4649-4654 ◽  

Fresh flowers of Gardenia carinata Wall. ex Roxb. were collected from Nonthaburi, Thailand. These plant materials were extracted by steam distillation extraction. The results showed that the percentage of essential oil from steam distillation was 0.16. The extracts obtained were subjected to gas chromatography/mass spectrometry for the identification of volatile constituents. The essential oil extracted by steam distillation was characterized by the presence of trans-geraniol (1, 19.9 %) and farnesol (2, 13.2 %) as the main component. The essential oil from flowers of G. carinata and two main components were evaluated for in vitro hyaluronidase inhibitory activity using fluorometric method and compared to a reference hyaluronidase inhibitor (6-O-palmitoylascorbic acid). The results were indicated that essential oil gave the mild inhibitory activity on hyaluronidase with IC50 of 1200.4 ± 21.1 g/mL as opposed to 6-O-palmitoylascorbic acid (IC50 =186.1 ± 3.9 g/mL). In the case of two main components, trans-geraniol (1) and farnesol (2) displayed moderate hyaluronidase inhibition activity with IC50 value at 535.7 ± 42.2 and 292.9 ± 23.4 g/mL, respectively. Additionally, in silico docking study of main component studies exhibited several important interactions between 1 and 2 and hyaluronidase binding site. Above finding confirmed the anti-hyaluronidase potential of G. carinata flowers.


2018 ◽  
Author(s):  
Min Liao ◽  
Qian-Qian Yang ◽  
Jin-Jing Xiao ◽  
Yong Huang ◽  
Li-Jun Zhou ◽  
...  

Background. In our previous study, Melaleuca alternifolia essential oil (EO) was considered to have an insecticidal effect by acting on the mitochondrial respiratory chain in insects. However, the mode of action is not fully understood. Methods. In this study, we investigated the insecticidal efficacy of the M. alternifolia EO against another major stored-product pest, Tribolium confusum Jacquelin du Val. Rarefaction and vacuolization of the mitochondrial matrix were evident in oil-fumigated T. confusum adults. Results. Alterations to the mitochondria confirmed the insecticidal effect of the M. alternifolia EO. Furthermore, comparative transcriptome analysis of T. confusum using RNA-seq indicated that most of the differentially expressed genes were involved in insecticide detoxification and mitochondrial function. The biochemical analysis showed that the intracellular NAD+/NADH ratio is involved in the differential effect of the M. alternifolia EO. Discussion. These results led us to conclude that NAD+/NADH dehydrogenase may be the prime target site for the M. alternifolia EO in insects, leading to blocking of the mitochondrial respiratory chain.


2019 ◽  
Vol 14 (1) ◽  
pp. 1934578X1901400
Author(s):  
Armando Cáceres ◽  
Sully M. Cruz ◽  
José V. Martínez-Arevalo ◽  
Amelia T. Henriques ◽  
Miriam A. Apel

Piper jacquemontianum Kunth (Piperaceae) is a native species from Central America and the Caribbean lowlands. It is traditionally used as a medicine and condiment in several ethnic groups from the region. Essential oils of dry leaves were obtained from eight ecologically different sites of Guatemala, which were cultivated under similar conditions in the Pacific basin. The essential oil yields obtained by hydrodistillation varied with the site of collection (0.3-1.7%). The essential oil composition was determined by gas chromatography; E-nerolidol was the only compound common to all provenances, although in a wide range (5.7-73.8%), being the main component of four of them. In the oils from other provenances, the main components were linalool, terpinen-4-ol, spathulenol, and α-bisabolol. This is the first report on the variation of the essential oil composition of this species of different provenance cultivated under similar conditions, suggesting high variability in its composition, and demonstrating at least five groups of P. jacquemontianum according to the composition of its essential oil. In further agrotechnological research, selection of the most promising samples could generate unique cultivars which might produce the expected compound.


2021 ◽  
Author(s):  
Samira Enayati ◽  
Mahdi Davari ◽  
Aziz Habibi-Yangjeh ◽  
Asgar Ebadollahi ◽  
Solmaz Feizpoor

Abstract Fusarium is one of the most important and destructive phytopathogenic fungi on a wide range of host plants. In the present study, to achieve a suitable alternative for high-risk synthetic chemicals, the antifungal effects of ZnO and ZnO-EO (Zataria multiflora Boiss essential oil loaded on ZnO) materials were investigated against six isolates of Fusarium. The chemical composition of Z. multiflora essential oil (EO) was explored by GC-MS, in which thymol and carvacrol were the main components. The physio-chemical properties of fabricated materials were studied by SEM, BET, FT-IR, TGA, EDX, XRD, and DLS analyses. The mycelial growth inhibitory (MGI) of ZnO and ZnO-EO materials were tested against Fusarium oxysporum f.sp. lycopersici, F. oxysporum f.sp. lentis, F. graminearum, F. graminearum, F. verticillioides, and F. brasilicum in the laboratory conditions. The results showed that ZnO-EO nanocomposite had a fungistatic effect against all tested fungi except F. oxysporum f.sp. lentis and the fungicidal activity against F. graminearum at a concentration of 1000 ppm. The MGI of ZnO-EO nanocomposite was increased by 42.70% compared to the pure ZnO and by 66.33% compared to Z. multiflora EO. The MGI of pure ZnO compared to Z. multiflora EO was also increased by 23.63%. According to the current findings, the ZnO-EO nanocomposite can be considered as a bio-rational efficient alternative to conventional chemical fungicides.


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