scholarly journals Impact of Adding Polysaccharides on the Stability of Egg Yolk/Fish Oil Emulsions under Accelerated Shelf-Life Conditions

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4020
Author(s):  
Selene Yadira Gonzalez Toledo ◽  
Jianping Wu
Keyword(s):  
High Pressure ◽  
Fish Oil ◽  
Encapsulation Efficiency ◽  
Gum Arabic ◽  
Egg Yolk ◽  
High Pressure Homogenization ◽  
Omega 3 ◽  
Significant Difference ◽  
Oil Emulsions ◽  
Over Time

Polysaccharides can form interfacial complexes with proteins to form emulsions with enhanced stability. We assessed the effect of adding gum guar or gum arabic to egg yolk/fish oil emulsions. The emulsions were produced using simple or high-pressure homogenization, stored for up to 10 days at 45 °C, and characterized for their particle size and distribution, viscosity, encapsulation efficiency, oxidative stability, and cytotoxicity. Emulsions containing gum guar and/or triglycerides had the highest viscosity. There was no significant difference in the encapsulation efficiency of emulsions regardless of the polysaccharide used. However, emulsions containing gum arabic displayed a bridging flocculation effect, resulting in less stability over time compared to those using gum guar. Emulsions produced using high-pressure homogenization displayed a narrower size distribution and higher stability. The formation of peroxides and propanal was lower in emulsions containing gum guar and was attributed to the surface oil. No significant toxicity toward Caco-2 cells was found from the emulsions over time. On the other hand, after 10 days of storage, nonencapsulated fish oil reduced the cell viability to about 80%. The results showed that gum guar can increase the particle stability of egg yolk/fish oil emulsions and decrease the oxidation rate of omega-3 fatty acids.

scholarly journals Buttermilk as Encapsulating Agent: Effect of Ultra-High-Pressure Homogenization on Chia Oil-in-Water Liquid Emulsion Formulations for Spray Drying

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1059
Author(s):  
Fatemeh Aghababaei ◽  
Mary Cano-Sarabia ◽  
Antonio J. Trujillo ◽  
Joan M. Quevedo ◽  
Victoria Ferragut
Keyword(s):  
High Pressure ◽  
Spray Drying ◽  
Encapsulation Efficiency ◽  
Morphological Characteristics ◽  
High Pressure Homogenization ◽  
Omega 3 ◽  
Potential Measurement ◽  
Ultra High Pressure ◽  
Oil In Water ◽  
Functional Components

Functional foods are highly demanded by consumers. Omega-3 rich oil and commercial buttermilk (BM), as functional components, used in combination to produce emulsions for further drying may facilitate the incorporation to foods. Ultra-high-pressure homogenization (UHPH) has a great potential for technological and nutritional aspects in emulsions production. The present study aimed to examine the potential improvement of UHPH technology in producing buttermilk-stabilized omega-3 rich emulsions (BME) for further drying, compared with conventional homogenization. Oil-in-water emulsions formulated with 10% chia: sunflower oil (50:50); 30% maltodextrin and 4 to 7% buttermilk were obtained by using conventional homogenization at 30 MPa and UHPH at 100 and 200 MPa. Particle size analysis, rheological evaluation, colloidal stability, zeta-potential measurement, and microstructure observations were performed in the BME. Subsequent spray drying of emulsions were made. As preliminary approximation for evaluating differences in the homogenization technology applied, encapsulation efficiency and morphological characteristics of on spray-dried emulsions (SDE) containing 21.3 to 22.7% oil content (dry basis) were selected. This study addresses the improvement in stability of BME treated by UHPH when compared to conventional homogenization and the beneficial consequences in encapsulation efficiency and morphology of SDE.

scholarly journals Effects of Industrial Waste Fish Oil Administration on Interleukin-6 (Il-6) Expression at Mice Colon being Induced by Azoxymethane (AOM) and Dextran Sodium Sulphate (DSS)

2021 ◽  
Vol 14 (4) ◽  
pp. 2227-2233
Author(s):  
Kevin Tjoa ◽  
Kusmardi Kusmardi ◽  
Yurnadi Hanafi Midoen
Keyword(s):  
Fish Oil ◽  
Industrial Waste ◽  
Positive Control ◽  
Negative Control ◽  
High Dose ◽  
Omega 3 ◽  
Colon Tissue ◽  
Significant Difference ◽  
Solvent Control ◽  
Medium Dose

Colorectal cancer (CRC) is the world’s third most cancer and the second highest mortality rate. The searching for new anti-inflammation substances with less adverse effects than aspirin for chemoprevention and adjuvant chemotherapy of CRC is running. The most notable one is fish oil containing omega 3. Kusmardi, et al. studied that industrial waste fish oil omega-3 level comes close enough to conventional fish oil industry. Study aims to reducing the level IL-6 on mice colon tissue being induced CRC using AOM/DSS by fish oil administration. Thirty male Swiss Webster mice are grouped into six treatments: positive control (aspirin), negative control (physiological saline), normal, high dose (fish oil 6mg/kgBW), medium dose (fish oil 3mg/kgBW), dan solvent control (corn oil). Colon tissue was stained using anti IL-6 antibody. Ten photos per slide were taken by microscope (400x), analyzed for the IL-6 expression by ImageJ®, and quantified for H-score. Data was analyzed using SPSS 24.0 (CI 95%) and p-value <0.05 is consider significant. Data are not normally distributed with median of 161.64 (119.4-260.67). Kruskal-Wallis test is significant in addition with Mann-Whitney test shows only high dose group has significant difference to negative control (p=0.008), medium dose (p=0.016) dan and solvent control (p=0.008). No significant difference reported between high dose and positive control group (p=0.69). High dose industrial waste fish oil can lower IL-6 expression in mice colon tissue induced CRC using AOM/DSS.

scholarly journals The Effect of pH and High-Pressure Homogenization on Droplet Size

2017 ◽  
Vol 2 (4) ◽  
pp. 110-122
Author(s):  
Ah Pis Yong ◽  
Md. Aminul Islam ◽  
Nurul Hasan
Keyword(s):  
High Pressure ◽  
Light Scattering ◽  
Droplet Size ◽  
Skim Milk ◽  
Egg Yolk ◽  
Continuous Phase ◽  
High Pressure Homogenization ◽  
Emulsion Droplets ◽  
Droplet Sizes ◽  
Basil Oil

The aims of this study are to revisit the effect of high pressure on homogenization and the influence of pH on the emulsion droplet sizes. The high-pressure homogenization (HPH) involves two stages of processing, where the first stage involves in blending the coarse emulsion by a blender, and the second stage requires disruption of the coarse emulsion into smaller droplets by a high-pressure homogenizer. The pressure range in this review is in between 10-500 MPa. The homogenised droplet sizes can be reduced by increasing the homogenization recirculation, and there is a threshold point beyond that by applying pressure only, the size cannot be further reduced. Normally, homogenised emulsions are classified by their degree of kinetic stability. Dispersed phase present in the form of droplets while continuous phase also known as suspended droplets. With a proper homogenization recirculation and pressure, a more kinetically stable emulsion can be produced. The side effects of increasing homogenization pressure are that it can cause overprocessing of the emulsion droplets where the droplet sizes become larger rather than the expected smaller size. This can cause kinetic instability in the emulsion. The droplet size is usually measured by dynamic light scattering or by laser light scattering technique. The type of samples used in this reviews are such as chocolate and vanilla based powders; mean droplet sizes samples; basil oil; tomato; lupin protein; oil; skim milk, soymilk; coconut milk; tomato homogenate; corn; egg-yolk, rapeseed and sunflower; Poly(4-vinylpyridine)/silica; and Complex 1 until complex 4 approaches from author case study. A relationship is developed between emulsion size and pH. Results clearly show that lower pH offers smaller droplet of emulsion and the opposite occurs when the pH is increased.

scholarly journals BMP-2 Expression of Post Tooth Extraction that Catfish Oil Application

2017 ◽  
Vol 3 (6) ◽  
pp. 69
Author(s):  
Theresia Indah B. ◽  
Bambang Sumaryono ◽  
Ketut Suardita ◽  
Amelia Putri R.
Keyword(s):  
Fish Oil ◽  
Healing Process ◽  
Clarias Batrachus ◽  
Bone Morphogenetic Protein 2 ◽  
Dental Extraction ◽  
Control Group ◽  
Wound Healing Process ◽  
Omega 3 ◽  
Significant Difference ◽  
Catfish Oil

Catfish (Clarias batrachus) oil contain the highest amount of omega-3-PUFA among other freshwater fish. The omega-3-PUFA in fish oil produced eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). It is known that EPA and DHA in essentials fatty acid (EFA) could improve BMP-2 expression. Bone morphogenetic protein-2 (BMP-2) is bone stimulator which capable of inducing differentiation of mesenchymal cells into osteoblast, stimulating bone formation in wound healing process of dental extraction. Purpose: To prove the increasing expression of BMP-2 after catfish (Clarias batrachus) oil application. Methods: We have used the post test only design in this research. There have been 21 Rattus novergicus as research samples, and those were divided into 3 groups, group KK as control, group KP1 was given catfish (Clarias batrachus) oil in 5% concentration, and group KP2 was given catfish (Clarias batrachus) oil in 10% concentration. Catfish (Clarias batrachus) oil were applied into the socket of dental extraction. Rat was decapulated 7 days after fish oil application and the jaw in the treated regions and control group were cut for immunohistochemistry examination to observe BMP-2 expression. Data was analyzed using one-way ANOVA test.  Result: There is significant difference increased of BMP-2 expression between control and treatment group. In samples given with 10% concentration of catfish oil had the most significant increase of BMP-2 expression. Conclusion: Catfish (Clarias batrachus) oil in 10% concentration could increase the expression of BMP-2 post dental extraction.Key words: Catfish oil, BMP-2, dental extraction.

scholarly journals EFFECT OF OMEGA 3 ON THE REPRODUCTIVE PERFORMANCE OF THE ALGERIAN SYNTHETIC RABBIT IN ARTIFICIAL INSEMINATION

AGROFOR ◽  
2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Khedidja BOUDOUR ◽  
El hassen LANKRI ◽  
Ahmed AICHOUNI ◽  
Nacira ZERROUKI ◽  
Mokhtar SAIDI
Keyword(s):  
Fish Oil ◽  
Reproductive Performance ◽  
Artificial Insemination ◽  
Marked Improvement ◽  
Animal Origin ◽  
Omega 3 ◽  
Live Births ◽  
Significant Difference

In the present work of the pubescent and immature rabbit does of the Algerian synthetic strain (ITELV 2006), the animals received a supplement of omega 3 of animal origin (fish oil) or vegetable origin (linseed) for 3 months (2 months before AI and 1 month during pregnancy). The females were followed until parturition in order to study the effect of omega 3 on their reproductive performance (fertility, prolificacy and stillbirth). Comparison of data between the six lots (Control puberts (CP), Control peripubertal (CPE), Linen grains pubertal (LGP), Linen grains peripubertal (LGPE), Fish oil puberts (FOP) and Fish oil peripubertal (FOPE)) for parameters of born deaths (BD), fertility and stillbirth rates showed no significant difference (P>0.05). Whereas for total births (TB), live births (LB) and weaner births (WB), the differences are significant (P0.05). A very marked improvement in prolificity has been achieved by the incorporation of fish oil and flaxseed into the feed of the rabbits. In fact, the females of these two lots recorded the highest values (10.37 TB, 9.87LB, 9.3 WB and 10.44 TB, 9.77LB, 8.77WB respectively for FOPE and LGPE), as opposed to the unpunished rabbits of the control lot which gave the lowest numbers (7.14TB, 6.57LB and 6.57WB).

Systematic Development and Optimization of an in-situ Gelling System for Moxifloxacin Ocular Nanosuspension using High-pressure Homogenization with an Improved Encapsulation Efficiency

2018 ◽  
Vol 24 (13) ◽  
pp. 1434-1445 ◽  
Author(s):  
Lalit Kumar Khurana ◽  
Romi Singh ◽  
Harinder Singh ◽  
Manju Sharma
Keyword(s):  
High Pressure ◽  
Process Parameters ◽  
Encapsulation Efficiency ◽  
Quality By Design ◽  
Polynomial Equation ◽  
Single Type ◽  
Application Site ◽  
High Pressure Homogenization ◽  
In Situ Gelling

Background: The objective of this study was to apply Quality by Design (QbD) principles on process parameter optimization for the development of hybrid delivery system (combination of (SLNs) and In-situ gelling system) for hydrophilic drug Moxifloxacin Hydrochloride (MOX) to achieve its controlled delivery, which otherwise may not be possible through single type of technology. Methods: Risk assessment studies were carried out to identify probable risks influencing CQAs on the product. In design of experiments (DoE), the process parameters (independent variables) i.e., chiller temperature X1, High Pressure Homogenization (HPH) pressure X2, and HPH cycles X3 were optimized using a three-factor two level face-centered central composite design to streamline the influence on three responses, namely encapsulation efficiency Y1, particle size Y2 and outlet temperature Y3. Independent and dependent variables were analyzed to establish a full-model second-order polynomial equation. F value is used to confirm the omission of insignificant parameters/interactions to derive a reduced-model polynomial equation to predict the Y1, Y2 and Y3 for optimized moxifloxacin in situ gelled nanosuspension. Results: Desirability plots showed the effects of X1, X2, and X3 on Y1, Y2 and Y3, respectively. The design space is generated to obtain optimized process parameters viz. chiller temperature (-5°C), HPH pressure 800 – 900 bar and 8 cycles that resulted in nanosuspension with ≈ 500 nm size, encapsulation efficiency >65% and final formulation temperature <23°C that were necessary to maintain the formulation in a liquid state. Conclusion: Quality by Design (QbD) approach is recently been encouraged by regulatory bodies to improve the quality of the finished product. This approach proved to be a useful tool in the development of robust nanosuspension of highly hydrophilic drugs with improved efficiency. Results indicate that such hybrid gel systems can be used to control the release of SLNs from application site and prolong their action in a sustained manner.

Inactivation of Alicyclobacillus acidoterrestris Using High Pressure Homogenization and Dimethyl Dicarbonate

2013 ◽  
Vol 76 (6) ◽  
pp. 1041-1045 ◽  
Author(s):  
WEI CHEN ◽  
FEDERICO M. HARTE ◽  
P. M. DAVIDSON ◽  
DAVID A. GOLDEN
Keyword(s):  
High Pressure ◽  
High Pressure Homogenization ◽  
Combined Effects ◽  
Vegetative Cells ◽  
Alicyclobacillus Acidoterrestris ◽  
Log Reduction ◽  
Significant Difference ◽  
Spore Population ◽  
Resistant Strains ◽  
Inactivation Effect

Vegetative cells and spores of five strains of Alicyclobacillus acidoterrestris (N-1100, N-1108, N-1096, SAC, and OS-CAJ) were screened for their sensitivity to high pressure homogenization (HPH, 0 to 300 MPa) in Bacillus acidoterrestris thermophilic broth. The most and least resistant strains, SAC and OS-CAJ, respectively, were further tested for their sensitivity to inactivation or growth inhibition by dimethyl dicarbonate (DMDC, 250 ppm). The combined effects of HPH and DMDC were then evaluated against SAC spores over a 24-h period after treatment. HPH alone significantly inactivated (P &lt; 0.05) vegetative cells of all five strains. SAC vegetative cells were least affected by HPH, with only about a 0.5-log reduction after the 300-MPa treatment. Spores were not significantly reduced by HPH for any of the five strains. DMDC reduced the initial vegetative cell population by 2 log CFU/ml and significantly increased the time to reach stationary phase. For spores, a 0.5-log decrease from the initial spore population was achieved and growth was not significantly delayed. No significant difference was found between the two strains. Treatment with DMDC plus HPH slightly enhanced the inactivation effect over a 24-h period compared with treatment with HPH alone, but these differences were statistically inconsistent. Although HPH and DMDC treatments may help control vegetative cells of A. acidoterrestris, these treatments may not provide adequate overall control. Neither treatment, alone or in combination, is very effective against spores.

Sign in / Sign up

Export Citation Format

Share Document