scholarly journals Influence of the Medium Composition and the Culture Conditions on Surfactin Biosynthesis by a Native Bacillus subtilis natto BS19 Strain

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2985
Author(s):  
Beata Koim-Puchowska ◽  
Grzegorz Kłosowski ◽  
Joanna Maria Dróżdż-Afelt ◽  
Dawid Mikulski ◽  
Alicja Zielińska
Keyword(s):  
Surface Tension ◽  
Bacillus Subtilis ◽  
Culture Medium ◽  
Synthetic Medium ◽  
Culture Conditions ◽  
Medium Composition ◽  
Native Strain ◽  
Medium Components ◽  
Bacillus Subtilis Natto ◽  
The Impact

An effective microbial synthesis of surfactin depends on the composition of the culture medium, the culture conditions and the genetic potential of the producer strain. The aim of this study was to evaluate the suitability of various medium components for the surfactin producing strain and to determine the impact of the culture conditions on the biosynthesis of surfactin isoforms by the newly isolated native strain Bacillus subtilis natto BS19. The efficiency of surfactin biosynthesis was determined by measuring the surface tension of the medium before and after submerged culture (SmF) and by qualitative and quantitative analysis of the obtained compound by high performance liquid chromatography. The highest efficiency of surfactin biosynthesis was achieved using starch as the carbon source and yeast extract as the nitrogen source at pH 7.0 and 37 °C. Potato peelings were selected as an effective waste substrate. It was shown that the increase in the percentage of peel extract in the culture medium enhanced the biosynthesis of surfactin (mg/L) (2–30.9%; 4–46.0% and 6–58.2%), while reducing surface tension of the medium by about 50%. The obtained results constitute a promising basis for further research on biosynthesis of surfactin using potato peelings as a cheap alternative to synthetic medium components.

scholarly journals Optimisation of Synthetic Medium Composition for Levorin Biosynthesis by Streptomyces levoris 99/23 and Investigation of its Accumulation Dynamics Using Mathematical Modelling Methods

2010 ◽  
Vol 59 (3) ◽  
pp. 179-183
Author(s):  
VESELIN S. STANCHEV ◽  
LUBKA Y. KOZHUHAROVA ◽  
BORIANA Y. ZHEKOVA ◽  
VELIZAR K. GOCHEV
Keyword(s):  
Mathematical Modelling ◽  
Culture Medium ◽  
Synthetic Medium ◽  
Equation System ◽  
Medium Composition ◽  
Antibiotic Biosynthesis ◽  
Medium Components ◽  
Linear Differential ◽  
Modelling Methods ◽  
Streptomyces Levoris

The composition of a synthetic culture medium for levorin biosynthesis by Streptomyces levoris 99/23 was optimised using mathematical modelling methods. The optimal concentrations of the medium components were established by means of an optimum composition design at three factor variation levels. An adequate regression model was obtained. Levorin biosynthesis by Streptomyces levoris 99/23 in the optimised synthetic medium was over 38% higher than in the initial medium. The antibiotic biosynthesis dynamics in the optimised culture medium was studied by means of a non-linear differential equation system. The resultant model was valid.

Production and control of extracellular enzymes in Micrococcus sodonensis

1974 ◽  
Vol 20 (1) ◽  
pp. 81-90 ◽  
Author(s):  
Cecily Mills ◽  
J. N. Campbell
Keyword(s):  
Alkaline Phosphatase ◽  
Inorganic Phosphate ◽  
Culture Medium ◽  
Extracellular Enzymes ◽  
Synthetic Medium ◽  
Culture Conditions ◽  
The Other ◽  
Organic Nutrients ◽  
And Control ◽  
Logarithmic Growth

Micrococcus sodonensis has been shown to produce several extracellular enzymes: an alkaline phosphatase, at least two forms of phosphodiesterase, a 5′-nucleotidase, and an alkaline proteinase. The quantitative release of these enzymes into the culture medium during logarithmic growth under all the various culture conditions tested indicates that these enzymes are truly extracellular in nature. Inorganic phosphate repressed the production of the alkaline phosphatase in synthetic as well as in complex media, whereas, the repression of the production of active diesterase and 5′-nucleotidase by inorganic phosphate was partly reversed by the addition of supplemental organic nutrients to the culture medium. Proteinase production was independent of the culture conditions used. A mutant strain of M. sodonensis with an altered production of diesterase was obtained; the other extracellular enzymes were unaffected. These results suggest that the extracellular enzymes of M. sodonensis are not produced in a pleiotropic fashion since the level of one of the enzymes can be changed without affecting a corresponding change in the levels of the other enzymes. An extracellular high molecular weight carbohydrate fraction was shown to be produced by M. sodonensis in synthetic medium. The fraction was also shown to contain glycoprotein.

scholarly journals Production of Enzymes from Agroindustrial Wastes by Biosurfactant-Producing Strains of Bacillus subtilis

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Francisco Fábio Cavalcante Barros ◽  
Ana Paula Resende Simiqueli ◽  
Cristiano José de Andrade ◽  
Gláucia Maria Pastore
Keyword(s):  
Bacillus Subtilis ◽  
Culture Medium ◽  
Synthetic Medium ◽  
Lipase Production ◽  
Industrial Processes ◽  
Technical Literature ◽  
Wide Range ◽  
Cassava Wastewater ◽  
Agroindustrial Wastes ◽  
Better Than

Bacteria in the genus Bacillus are the source of several enzymes of current industrial interest. Hydrolases, such as amylases, proteases, and lipases, are the main enzymes consumed worldwide and have applications in a wide range of products and industrial processes. Fermentation processes by Bacillus subtilis using cassava wastewater as a substrate are reported in the technical literature; however, the same combination of microorganisms and this culture medium is limited or nonexistent. In this paper, the amylase, protease, and lipase production of ten Bacillus subtilis strains previously identified as biosurfactant producers in cassava wastewater was evaluated. The LB1a and LB5a strains were selected for analysis using a synthetic medium and cassava wastewater and were identified as good enzyme producers, especially of amylases and proteases. In addition, the enzymatic activity results indicate that cassava wastewater was better than the synthetic medium for the induction of these enzymes.

Optimisation of Tissue Culture Conditions for Transformation Studies Using Immature Embryos of Australian Barley Cultivars

1995 ◽  
Vol 43 (5) ◽  
pp. 499 ◽  
Author(s):  
AM Walmsley ◽  
RJ Henry ◽  
RG Birch
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Culture Medium ◽  
Immature Embryo ◽  
Culture Conditions ◽  
Medium Composition ◽  
Immature Embryos ◽  
Systematic Testing ◽  
Barley Cultivars ◽  
Key Variables

Eight Australian barley cultivars were tested for efficiency of embryonic callus initiation and plant regeneration, from immature embryo explants in tissue culture. Optimisation of tissue culture conditions was performed for cultivars Bandulla, Clipper, Schooner and Tallon in an attempt to increase regeneration frequencies to levels suitable for genetic engineering of barley. Variables tested were 2,4-D concentration, salt composition, carbon source and immature embryo explant. Optimal culture medium composition varied between cultivars. Shoot regeneration rates from culture of isolated scutellar tissues were low for all four cultivars. Halved, immature embryos produced most shoots for cultivars Clipper, Schooner and Tallon, whereas Bandulla performed best with entire immature embryo explants. Clipper (a malting barley) and Bandulla (a feed barley) are suggested as model Australian cultivars for transformation studies. Immature embryos of Bandulla produced an average of 5.3 shoots and Clipper 10.1 shoots per embryo under optimal conditions. Our results show that rates of somatic embryo and plant regeneration sufficient for use in transformation studies can be achieved for diverse Australian Barley cultivars, through systematic testing of a range of key variables including explant type and medium composition.

Improved growth and viability of lactobacilli in the presence of Bacillus subtilis (natto), catalase, or subtilisin

2000 ◽  
Vol 46 (10) ◽  
pp. 892-897 ◽  
Author(s):  
Tomohiro Hosoi ◽  
Akio Ametani ◽  
Kan Kiuchi ◽  
Shuichi Kaminogawa
Keyword(s):  
Hydrogen Peroxide ◽  
Bacillus Subtilis ◽  
Culture Medium ◽  
Lactobacillus Reuteri ◽  
Promoting Effect ◽  
Viable Cells ◽  
Bacillus Subtilis Natto ◽  
Growth Promoting ◽  
Similar Growth

In an effort to demonstrate the potential usefulness of Bacillus subtilis (natto) as a probiotic, we examined the effect of this organism on the growth of three strains of lactobacilli co-cultured aerobically in vitro. Addition of B. subtilis (natto) to the culture medium resulted in an increase in the number of viable cells of all lactobacilli tested. Since B. subtilis (natto) can produce catalase, which has been reported to exhibit a similar growth-promoting effect on lactobacilli, we also examined the effect of bovine catalase on the growth of Lactobacillus reuteri JCM 1112 and L. acidophilus JCM 1132. Both catalase and B. subtilis (natto) enhanced the growth of L. reuteri JCM 1112, whereas B. subtilis (natto) but not catalase enhanced the growth of L. acidophilus JCM 1132. In a medium containing 0.1 mM hydrogen peroxide, its toxic effect on L. reuteri JCM 1112 was abolished by catalase or B. subtilis (natto). In addition, a serine protease from B. licheniformis, subtilisin, improved the growth and viability of L. reuteri JCM 1112 and L. acidophilus JCM 1132 in the absence of hydrogen peroxide. These results indicate that B. subtilis (natto) enhances the growth and (or) viability of lactobacilli, possibly through production of catalase and subtilisin.Key words: Bacillus subtilis (natto), Lactobacillus, probiotic, catalase, subtilisin.

scholarly journals Each Mycobacterium Requires a Specific Culture Medium Composition for Triggering an Optimized Immunomodulatory and Antitumoral Effect

2020 ◽  
Vol 8 (5) ◽  
pp. 734
Author(s):  
Sandra Guallar-Garrido ◽  
Víctor Campo-Pérez ◽  
Alejandro Sánchez-Chardi ◽  
Marina Luquin ◽  
Esther Julián
Keyword(s):  
Culture Medium ◽  
Medium Composition ◽  
Patient Treatment ◽  
Bacillus Calmette Guerin ◽  
Mycobacterial Species ◽  
Muscle Invasive Bladder Cancer ◽  
Specific Culture ◽  
Muscle Invasive ◽  
The Impact ◽  
Insight Into

Mycobacterium bovis bacillus Calmette-Guérin (BCG) remains the first treatment option for non-muscle-invasive bladder cancer (BC) patients. In research laboratories, M. bovis BCG is mainly grown in commercially available media supplemented with animal-derived agents that favor its growth, while biomass production for patient treatment is performed in Sauton medium which lacks animal-derived components. However, there is not a standardized formulation of Sauton medium, which could affect mycobacterial characteristics. Here, the impact of culture composition on the immunomodulatory and antitumor capacity of M. bovis BCG and Mycolicibacterium brumae, recently described as efficacious for BC treatment, has been addressed. Both mycobacteria grown in Middlebrook and different Sauton formulations, differing in the source of nitrogen and amount of carbon source, were studied. Our results indicate the relevance of culture medium composition on the antitumor effect triggered by mycobacteria, indicating that the most productive culture medium is not necessarily the formulation that provides the most favorable immunomodulatory profile and the highest capacity to inhibit BC cell growth. Strikingly, each mycobacterial species requires a specific culture medium composition to provide the best profile as an immunotherapeutic agent for BC treatment. Our results highlight the relevance of meticulousness in mycobacteria production, providing insight into the application of these bacteria in BC research.

scholarly journals Design of a culture medium for optimal growth of the bacterium Pseudoxanthomonas indica H32 allowing its production as biopesticide and biofertilizer

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Dayana Morales-Borrell ◽  
Nemecio González-Fernández ◽  
Néstor Mora-González ◽  
Carlos Pérez-Heredia ◽  
Ana Campal-Espinosa ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Yeast Extract ◽  
Culture Medium ◽  
Nitrogen Sources ◽  
Optimal Growth ◽  
Culture Conditions ◽  
Medium Composition ◽  
Mineral Salts ◽  
Molar Ratios ◽  
Biotechnological Processes

Abstract Culture medium composition is one of the most important parameters to analyze in biotechnological processes with industrial purposes. The aim of this study was to design of a culture medium for optimal growth of the bacterium Pseudoxanthomonas indica H32 allowing its production as biopesticide and biofertilizer. The influence of several carbon and nitrogen sources and their molar ratios on P. indica H32 growth was investigated. The effect of different micronutrients such as mineral salts and vitamin on P. indica H32 growth was determined as well. A mixture design based on Design-Expert 10.0 Software was performed to optimize the culture medium concentration. Finally, in the designed medium, an attribute of the biological mechanism of action of the P. indica H32 against nematodes, was evaluated: the hydrogen sulfide production. It was found that tested carbon/nitrogen ratios were not a significant influence on P. indica H32 growth. Growth of P. indica H32 was favored with use of sucrose, yeast extract and phosphate buffer without the addition of any tested micronutrients. An optimal concentration of 10 g/L sucrose and 5 g/L yeast extract were obtained at a cost of 0.10 $/L. In this concentration, the specific growth rate (µ) and maximal optical density (Xmax) were equal to 0.439 h− 1 and 8.00 respectively. It was evidenced that under the culture conditions used, P. indica H32 produced hydrogen sulfide. The designed medium led to a 1.08 $/L reduction of costs in comparison to LB medium. These results were critical to carry on with biotechnological development of P. indica H32 as a bioproduct.

Effect of medium components and culture conditions in Bacillus subtilis EA-CB0575 spore production

2015 ◽  
Vol 38 (10) ◽  
pp. 1879-1888 ◽  
Author(s):  
Luisa F. Posada-Uribe ◽  
Magally Romero-Tabarez ◽  
Valeska Villegas-Escobar
Keyword(s):  
Bacillus Subtilis ◽  
Culture Conditions ◽  
Spore Production ◽  
Medium Components
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