scholarly journals Synthesis and Nano-Sized Characterization of Bioactive Oregano Essential Oil Molecule-Loaded Small Unilamellar Nanoliposomes with Antifungal Potentialities

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2880
Author(s):  
Katya M. Aguilar-Pérez ◽  
Dora I. Medina ◽  
Jayanthi Narayanan ◽  
Roberto Parra-Saldívar ◽  
Hafiz M. N. Iqbal
Keyword(s):  
Biological Activity ◽  
Essential Oil ◽  
Antifungal Agents ◽  
Imaging Techniques ◽  
Attenuated Total Reflection ◽  
Oregano Essential Oil ◽  
Size Characterization ◽  
Nano Size

The development of greener nano-constructs with noteworthy biological activity is of supreme interest, as a robust choice to minimize the extensive use of synthetic drugs. Essential oils (EOs) and their constituents offer medicinal potentialities because of their extensive biological activity, including the inhibition of fungi species. However, their application as natural antifungal agents are limited due to their volatility, low stability, and restricted administration routes. Nanotechnology is receiving particular attention to overcome the drawbacks of EOs such as volatility, degradation, and high sensitivity to environmental/external factors. For the aforementioned reasons, nanoencapsulation of bioactive compounds, for instance, EOs, facilitates protection and controlled-release attributes. Nanoliposomes are bilayer vesicles, at nanoscale, composed of phospholipids, and can encapsulate hydrophilic and hydrophobic compounds. Considering the above critiques, herein, we report the in-house fabrication and nano-size characterization of bioactive oregano essential oil (Origanum vulgare L.) (OEO) molecules loaded with small unilamellar vesicles (SUV) nanoliposomes. The study was focused on three main points: (1) multi-compositional fabrication nanoliposomes using a thin film hydration–sonication method; (2) nano-size characterization using various analytical and imaging techniques; and (3) antifungal efficacy of as-developed OEO nanoliposomes against Trichophyton rubrum (T. rubrum) by performing the mycelial growth inhibition test (MGI). The mean size of the nanoliposomes was around 77.46 ± 0.66 nm and 110.4 ± 0.98 nm, polydispersity index (PdI) of 0.413 ± 0.015, zeta potential values up to −36.94 ± 0.36 mV were obtained by dynamic light scattering (DLS). and spherical morphology was confirmed by scanning electron microscopy (SEM). The presence of OEO into nanoliposomes was displayed by attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy. Entrapment efficiency values of 79.55 ± 6.9% were achieved for OEO nanoliposomes. In vitro antifungal activity of nanoliposomes tested against T. rubrum strains revealed that OEO nanoliposomes exhibited the highest MGI, 81.66 ± 0.86%, at a concentration of 1.5 µL/mL compared to the rest of the formulations. In summary, this work showed that bioactive OEO molecules with loaded nanoliposomes could be used as natural antifungal agents for therapeutical purposes against T. rubrum.

Structural characterization of naphthalene sulfonamides and a sulfonate ester and their in vitro efficacy against Leishmania tarentolae promastigotes

2021 ◽  
Vol 45 (10) ◽  
pp. 4791-4801
Author(s):  
Edward W. Li ◽  
Jade Katinas ◽  
Marjorie A. Jones ◽  
Christopher G. Hamaker
Keyword(s):  
Biological Activity ◽  
Structural Characterization ◽  
Leishmania Tarentolae ◽  
Sulfonate Ester

Structural and biological activity analyses of two naphthalene sulfonamides and a naphthalene sulfonate ester.

scholarly journals Physico-Chemical and Biological Evaluation of PLCL/SF Nanofibers Loaded with Oregano Essential Oil

Pharmaceutics ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 386 ◽  
Author(s):  
Atta ur Rehman Khan ◽  
Muhammad Nadeem ◽  
M. Aqeel Bhutto ◽  
Fan Yu ◽  
Xianrui Xie ◽  
...  
Keyword(s):  
Essential Oil ◽  
Biological Significance ◽  
Biological Evaluation ◽  
Attenuated Total Reflection ◽  
Surgical Removal ◽  
Physical Interaction ◽  
Oregano Essential Oil ◽  
Physico Chemical ◽  
Electrospun Membranes ◽  
Biphasic Release

Essential oils are complex volatile compounds, extracted from specific plant species, with promising therapeutic potentials. However, their volatile nature presents a major hindrance in using them as therapeutic agents. In the current study, we successfully encapsulated oregano essential oil (OEO) into Poly (l-lactic acid-co-e-caprolactone) /Silk Fibroin (PLCL/SF) polymers through electrospinning. The nanofibrous membrane (NF) was fabricated and characterized for various physico-chemical and biological attributions. Homogenous and bead free morphology was confirmed by scanning electron microscopy (SEM). Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) confirmed the successful loading of OEO and its physical interaction with the blend of PLCL/SF. Moreover, thermogravimetric analysis (TGA) also confirmed the successful loading and thermostability of the OEO. Although a significant change was noted in tensile strength due to the loading of OEO, the mechanical behaviour still falls into the acceptable ranges required for skin tissue engineering. Similarly, fabricated material was evaluated for its biological significance. Liquid chromatography-mass spectrometry (LC-MS) was employed to determine the release behaviour of OEO from electrospun membranes. LC-MS data, noted for 48 h, confirmed the biphasic release of OEO. Furthermore, NF membranes have shown strong antioxidant and anti-tumor activities. This material is promising and can be implanted to avoid the recurrence of the tumor after its surgical removal.

scholarly journals Individual and Combined Coatings of Chitosan and Carnauba Wax with Oregano Essential Oil to Avoid Water Loss and Microbial Decay of Fresh Cucumber

Coatings ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 614
Author(s):  
María Melissa Gutiérrez-Pacheco ◽  
Luis Alberto Ortega-Ramírez ◽  
Brenda Adriana Silva-Espinoza ◽  
Manuel Reynaldo Cruz-Valenzuela ◽  
Gustavo Adolfo González-Aguilar ◽  
...  
Keyword(s):  
Weight Loss ◽  
Essential Oil ◽  
Transmission Rate ◽  
Infrared Analysis ◽  
Absorption Bands ◽  
Oregano Essential Oil ◽  
Carnauba Wax ◽  
Bacteria And Fungi ◽  
Combined Coatings

The objective of the present study is to evaluate the effect of individual and combined coatings of chitosan (0.008 g·mL−1) and carnauba wax (0.1 g·mL−1) with oregano essential oil (OEO, 0.08 g·mL−1) to reduce dehydration and microbial decay of fresh cucumbers stored at 10 °C. Chitosan-OEO-wax films showed the lowest water vapor transmission rate (0.141 g·m−2·h−1), compared to single chitosan films (0.257 g·m−2·h−1). While chitosan-OEO films completely inhibited the in vitro growth of Alternaria alternata and reduced the growth of Salmonella Typhimurium, Escherichia coli O157:H7, mesophilic bacteria, and fungi isolated from decayed cucumbers. Besides, the infrared analysis of chitosan-OEO-wax films showed shifts in O–H and N–H absorption bands, indicating possible hydrogen bonding between the components. Wax and wax-OEO were the most effective coatings to prevent weight loss in cucumbers during 15 days of storage at 10 °C, while the most effective antimicrobial treatments were chitosan and chitosan-OEO. Therefore, these results showed that carnauba wax and carnauba wax-OEO coatings were the most effective in weight loss, whereas chitosan and chitosan-OEO were the most effective to reduce the microbial load of the treated fresh cucumber.

Synthesis and characterization of two novel dicyanidoargentate(i) complexes containing N-(2-hydroxyethyl)ethylenediamine exhibiting significant biological activity

2014 ◽  
Vol 38 (10) ◽  
pp. 4760-4773 ◽  
Author(s):  
Nesrin Korkmaz ◽  
Ahmet Karadağ ◽  
Ali Aydın ◽  
Yusuf Yanar ◽  
İsa Karaman ◽  
...  
Keyword(s):  
Biological Activity ◽  
Synthesis And Characterization ◽  
Anticancer Activities

Two novel cyanido-complexes having very significant antibacterial, antifungal and anticancer activities in vitro were synthesized and characterized using various techniques.

Immunological determination and size characterization of poly(ADP-ribose) synthesized in vitro and in vivo

1999 ◽  
Vol 1428 (2-3) ◽  
pp. 137-146 ◽  
Author(s):  
El Bachir Affar ◽  
Patrick J. Duriez ◽  
Rashmi G. Shah ◽  
Eric Winstall ◽  
Marc Germain ◽  
...  
Keyword(s):  
Size Characterization

Biological Activity of Abies Sibirica Essential Oil and its Major Constituents for Several Enzymes In Vitro

2014 ◽  
Vol 48 (7) ◽  
pp. 456-460 ◽  
Author(s):  
N. A. Polyakov ◽  
V. A. Dubinskaya ◽  
A. A. Efremov ◽  
E. A. Efremov
Keyword(s):  
Biological Activity ◽  
Essential Oil ◽  
Abies Sibirica

In vitro biological activity and essential oil composition of four indigenous South African Helichrysum species

2004 ◽  
Vol 95 (2-3) ◽  
pp. 253-258 ◽  
Author(s):  
A.C.U. Lourens ◽  
D. Reddy ◽  
K.H.C. Başer ◽  
A.M. Viljoen ◽  
S.F. Van Vuuren
Keyword(s):  
Biological Activity ◽  
Essential Oil ◽  
South African ◽  
Essential Oil Composition ◽  
Oil Composition

Biological characterization of charge isomers of human growth hormone

1988 ◽  
Vol 118 (1) ◽  
pp. 14-21 ◽  
Author(s):  
A. Skottner ◽  
A. Forsman ◽  
B. Skoog ◽  
J. L. Kostyo ◽  
C. M. Cameron ◽  
...  
Keyword(s):  
Biological Activity ◽  
Biological Activities ◽  
Human Growth ◽  
Proteolytic Processing ◽  
Biological Characterization ◽  
Growth Promoting ◽  
Rat Adipose Tissue ◽  
Insulin Like Activity

Abstract. Since deamidation of the human GH molecule may alter the manner and extent to which the hormone is cleaved by proteases, and since it has been repeatedly suggested that proteolytic processing is required for the expression of certain of the activities of GH, the present study was conducted to determine whether the biological activity profiles of more acidic forms of human GH are altered. Three charge isomers, GH-b, GH-c and GH-d, representing primarily deamidated forms, were isolated from a native human GH preparation (Crescormon®) in amounts adequate for characterization of their biological activities. All three were essentially equipotent in a radioimmunoassay for human GH. When assessed for growth-promoting activity in the hypophysectomized rat, the isomers were again equipotent with each other and with the GH preparation from which they were derived. The charge isomers also had significant in vitro insulin-like activity on isolated rat adipose tissue and diabetogenic activity in the ob/ob mouse. Thus, the biological activity profiles of these charge isomers of human GH do not differ greatly from one another.

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