scholarly journals Synthesis, Biomacromolecular Interactions, Photodynamic NO Releasing and Cellular Imaging of Two [RuCl(qn)(Lbpy)(NO)]X Complexes

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2545
Author(s):  
Luna Song ◽  
Hehe Bai ◽  
Chenyang Liu ◽  
Wenjun Gong ◽  
Ai Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Confocal Imaging ◽  
Epr Spectra ◽  
Distorted Octahedral Geometry ◽  
Ionization Mass ◽  
Time Resolved ◽  
Pbr322 Dna ◽  
Vis Spectroscopy ◽  
No Release ◽  
Ft Ir

Two light-activated NO donors [RuCl(qn)(Lbpy)(NO)]X with 8-hydroxyquinoline (qn) and 2,2′-bipyridine derivatives (Lbpy) as co-ligands were synthesized (Lbpy1 = 4,4′-dicarboxyl-2,2′-dipyridine, X = Cl− and Lbpy2 = 4,4′-dimethoxycarbonyl-2,2′-dipyridine, X = NO3−), and characterized using ultraviolet–visible (UV-vis) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, nuclear magnetic resonance (1H NMR), elemental analysis and electrospray ionization mass spectrometry (ESI-MS) spectra. The [RuCl(qn)(Lbpy2)(NO)]NO3 complex was crystallized and exhibited distorted octahedral geometry, in which the Ru–N(O) bond length was 1.752(6) Å and the Ru–N–O angle was 177.6(6)°. Time-resolved FT-IR and electron paramagnetic resonance (EPR) spectra were used to confirm the photoactivated NO release of the complexes. The binding constant (Kb) of two complexes with human serum albumin (HSA) and DNA were quantitatively evaluated using fluorescence spectroscopy, Ru-Lbpy1 (Kb~106 with HSA and ~104 with DNA) had higher affinity than Ru-Lbpy2. The interactions between the complexes and HSA were investigated using matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) and EPR spectra. HSA can be used as a carrier to facilitate the release of NO from the complexes upon photoirradiation. The confocal imaging of photo-induced NO release in living cells was successfully observed with a fluorescent NO probe. Moreover, the photocleavage of pBR322 DNA for the complexes and the effect of different Lbpy substituted groups in the complexes on their reactivity were analyzed.

Mixed-ligand copper(ii)-phenolate complexes: structure and studies on DNA/protein binding profiles, DNA cleavage, molecular docking and cytotoxicity

RSC Advances ◽  
2016 ◽  
Vol 6 (3) ◽  
pp. 1810-1825 ◽  
Author(s):  
Sellamuthu Kathiresan ◽  
Subramanian Mugesh ◽  
Maruthamuthu Murugan ◽  
Feroze Ahamed ◽  
Jamespandi Annaraj
Keyword(s):  
Mass Spectrometry ◽  
Molecular Docking ◽  
Electrospray Ionization ◽  
Elemental Analysis ◽  
Dna Cleavage ◽  
Mixed Ligand ◽  
Ionization Mass ◽  
Electrochemical Studies ◽  
Mixed Ligands ◽  
Ft Ir

Copper(ii) complexes with simple and mixed ligands, [Cu(L)(ClO4)] and [Cu(L)(diimine)]ClO4 were synthesized and characterized by elemental analysis, UV-vis, FT-IR, electrospray ionization-mass spectrometry (ESI-MS) and electrochemical studies.

A Mass Spectrometry Investigation of the Conformational Changes in Adrenocorticotropic Hormones

2002 ◽  
Vol 8 (5) ◽  
pp. 381-387 ◽  
Author(s):  
Hui Lin ◽  
Chhabil Dass
Keyword(s):  
Mass Spectrometry ◽  
Conformational Changes ◽  
Aqueous Media ◽  
Charge State Distribution ◽  
Random Coil ◽  
Helical Conformation ◽  
Ionization Mass ◽  
Time Resolved ◽  
Esi Ms ◽  
Α Helix

Electrospray ionization-mass spectrometry (ESI-MS) was employed to study methanol-induced conformational changes in adrenocorticotrophic hormone (ACTH). ACTH, a 39–residue peptide, is a member of the proopiomelanocortin family of peptides. Charge-state distribution (CSD) and hydrogen–deuterium (H/D) exchange were used to monitor the conformational changes as a function of methanol concentration. The latter experiments were conducted via time-resolved ESI-MS in a continuous-flow apparatus. The CSD and the H/D exchange experimental data both reveal that ACTH exists, presumably in a random coil open structure in aqueous media, but assumes a more compact helical conformation with increased concentration of methanol. The H/D exchange experiments also reveal that 79% of ACTH is present as α-helix in mixed water-methanol solvent media.

scholarly journals Some Novel Manganese(III) Mixed Ligand Complexes and its Decolourization Studies

2018 ◽  
Vol 34 (6) ◽  
pp. 2867-2871
Author(s):  
Deepika Jaiswal ◽  
Sudha Yadava
Keyword(s):  
Mass Spectrometry ◽  
Mixed Ligand ◽  
Mixed Ligand Complexes ◽  
Distorted Octahedral Geometry ◽  
Ligand Field ◽  
Distorted Octahedral ◽  
Ft Ir ◽  
Ethylenediamine Ligand ◽  
Red Dye ◽  
Ligand Field Parameters

Some novel mixed ligand complexes of Mn(III) with glycine ligand namely [Mn(gly)2 Cl(en)], [Mn(gly)2 Br(en)], [Mn(gly)2 N3 (en)] and [Mn(gly)2 NCS(en)] have been synthesized starting from Mn(gly)2 Cl, Mn(gly)2 Br, Mn(gly)2N3 and Mn(gly)2 NCS respectively. These newly synthesized complexes have been characterized by UV/Vis, FT-IR and Mass spectrometry. The spectroscopic data suggest distorted octahedral geometry for all these mixed ligand complexes. The λmax values of these complexes for 5T2g → 5Eg transitions are 482 nm for [Mn(gly)2N3(en)], 488 for [Mn(gly)2NCS(en)], 486 for [Mn(gly)2 Br(en)] and 484 for [Mn(gly)2Cl(en)], all these transitions are red shifted in comparison to their parent complexes. The ligand field parameters such as 10 Dq, B and β have also been calculated and suggest covalent metal ligand bonding. One peculiar finding is that the FT-IR spectra shows frequencies for both free and coordinated NH2 group in all complexes indicating that the ethylenediamine ligand present here is non bridging in nature. The mass spectrometry results show molecular ion peaks at m/z 300, 345, 307 and 323 for [Mn(gly)2Cl(en)], [Mn(gly)2Br(en)], [Mn(gly)2N3(en)] and [Mn(gly)2NCS(en)] respectively. The coordination of ethylenediamine to Mn(III) enhances its efficiency towards decolourization methyl red dye.

Time-resolved in situ monitoring of photocatalytic reactions by probe electrospray ionization mass spectrometry

The Analyst ◽  
2020 ◽  
Vol 145 (9) ◽  
pp. 3313-3319
Author(s):  
Zhongbao Han ◽  
Xiaoyu Gu ◽  
Shirong Wang ◽  
Liyan Liu ◽  
Ying Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Real Time ◽  
Electrospray Ionization Mass Spectrometry ◽  
In Situ Monitoring ◽  
Ionization Mass ◽  
Time Resolved ◽  
Photocatalytic Reactions ◽  
Probe Electrospray Ionization

We report the application of PESI-MS to in situ monitoring of photocatalytic reactions of cationic dyes in suspensions in real-time.

2003 Fred Beamish Award Lecture — Exploring the dynamics of biological systems by mass spectrometry

2004 ◽  
Vol 82 (11) ◽  
pp. 1565-1580 ◽  
Author(s):  
Lars Konermann
Keyword(s):  
Mass Spectrometry ◽  
Protein Interactions ◽  
High Throughput Screening ◽  
Structural Changes ◽  
Structural Information ◽  
Ionization Mass ◽  
Time Resolved ◽  
Rapid Mixing ◽  
Esi Ms ◽  
Compound Libraries

This review describes the use of electrospray ionization mass spectrometry (ESI-MS) in conjunction with on-line rapid mixing techniques. This combination, termed "time-resolved" ESI-MS, provides a powerful approach for studying solution-phase reactions on timescales as short as a few milliseconds. Of particular interest is the application of this technique for monitoring protein folding reactions. Time-resolved ESI-MS can provide detailed information on structural changes of the polypeptide chain, while at the same time probing the occurrence of noncovalent ligand–protein interactions. Especially when used in combination with hydrogen–deuterium pulse labeling, these measurements yield valuable structural information on short-lived folding intermediates. Similar approaches can be used to monitor the dynamics of proteins under equilibrium conditions. Another important application of time-resolved ESI-MS are mechanistic studies on enzyme-catalyzed processes. These reactions can be monitored under presteady-state conditions, without requiring artificial chromophoric substrates or radioactive labeling. We also discuss the use of ESI-MS for monitoring noncovalent ligand–protein interactions by diffusion measurements. In contrast to conventional MS-based techniques, this approach does not rely on the preservation of noncovalent interactions in the gas phase. It appears that diffusion measurements by ESI-MS could become an interesting alternative to existing methods for the high throughput screening of compound libraries in the context of drug discovery.Key words: reaction intermediate, rapid mixing, kinetics, protein conformation, protein function.

Sign in / Sign up

Export Citation Format

Share Document