scholarly journals Genome Mining, Heterologous Expression, Antibacterial and Antioxidant Activities of Lipoamides and Amicoumacins from Compost-Associated Bacillus subtilis fmb60

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1892
Author(s):  
Jie Yang ◽  
Qingzheng Zhu ◽  
Feng Xu ◽  
Ming Yang ◽  
Hechao Du ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Secondary Metabolites ◽  
Heterologous Expression ◽  
Gene Cluster ◽  
Broad Spectrum ◽  
Antioxidant Activities ◽  
Genome Mining ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
First Time

Bacillus subtilis fmb60, which has broad-spectrum antimicrobial activities, was isolated from plant straw compost. A hybrid NRPS/PKS cluster was screened from the genome. Sixteen secondary metabolites produced by the gene cluster were isolated and identified using LC-HRMS and NMR. Three lipoamides D–F (1–3) and two amicoumacin derivatives, amicoumacins D, E (4, 5), were identified, and are reported here for the first time. Lipoamides D–F exhibited strong antibacterial activities against harmful foodborne bacteria, with the MIC ranging from 6.25 to 25 µg/mL. Amicoumacin E scavenged 38.8% of ABTS+ radicals at 1 mg/mL. Direct cloning and heterologous expression of the NRPS/PKS and ace gene cluster identified its importance for the biosynthesis of amicoumacins. This study demonstrated that there is a high potential for biocontrol utilization of B. subtilis fmb60, and genome mining for clusters of secondary metabolites of B. subtilis fmb60 has revealed a greater biosynthetic potential for the production of novel natural products than previously anticipated.

scholarly journals Genome Mining and Heterologous Expression of Lipoamides and Amicoumacins From Compost-Associated Bacillus subtilis fmb60

2021 ◽  
Author(s):  
jie yang ◽  
Qingzheng Zhu ◽  
Feng Xu ◽  
Ming Yang ◽  
Hechao Du ◽  
...  
Keyword(s):  
Natural Products ◽  
Bacillus Subtilis ◽  
Secondary Metabolites ◽  
Heterologous Expression ◽  
Gene Cluster ◽  
Broad Spectrum ◽  
Genome Mining ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
First Time

Abstract: Bacillus subtilis fmb60, which has broad-spectrum antimicrobial activities was isolated from plant straw compost. A hybrid NRPS/PKS cluster was screened from the genome. Sixteen secondary metabolites produced by the gene cluster were isolated and identified using LC-HRMS and NMR. Three lipoamides D–F (1-3) and two amicoumacin derivatives, amicoumacins D, E (4, 5), were identified, and are reported here for the first time. Lipoamides D–F exhibited strong antibacterial activities against harmful foodborne bacteria, with the MIC ranging from 6.25 to 25 µg/mL. Amicoumacin E scavenged 38.8% of ABTS+ radicals at 1 mg/mL. Direct cloning and heterologous expression of the NRPS/PKS and ace gene cluster identified its importance for the biosynthesis of amicoumacins. This study demonstrated that there is a high potential for biocontrol utilization of B. subtilis fmb60, and genome mining for clusters of secondary metabolites of B. subtilis fmb60 has revealed a greater biosynthetic potential for the production of novel natural products than previously anticipated.

scholarly journals Identification and Verification of the Prodigiosin Biosynthetic Gene Cluster (BGC) in Pseudoalteromonas rubra S4059

2021 ◽  
Author(s):  
Xiyan Wang ◽  
Thomas Isbrandt ◽  
Emil Ørsted Christensen ◽  
Jette Melchiorsen ◽  
Thomas Ostenfeld Larsen ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Heterologous Expression ◽  
Gene Cluster ◽  
Genome Mining ◽  
Gene Clusters ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters ◽  
Pseudoalteromonas Rubra

Pigmented Pseudoalteromonas strains are renowned for their production of secondary metabolites, and genome mining has revealed a high number of biosynthetic gene clusters (BGCs) for which the chemistry is unknown. Identification of those BGCs is a prerequisite for linking products to gene clusters and for further exploitation through heterologous expression.

Polyphenol Contents, Antioxidant and Antibacterial Activities of Aqueous Algerian Propolis Extracts

2021 ◽  
Author(s):  
E.K. Soltani ◽  
K. Zaim ◽  
K. Mokhnache ◽  
N. Haichour ◽  
S. Mezaache-Aichour ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Natural Antioxidants ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Biological Properties ◽  
Aqueous Extracts

The propolis, an extremely complex resinous material, exhibits valuable pharmacological and biological properties, mainly attributed to the presence of polyphenols. The composition of propolis depends on time, vegetation, and the area of collection. Total flavonoid and polyphenol contents of aqueous extracts of propolis samples from different areas of Algeria, determined by using aluminum chloride and Folin–Ciocalteu colorimetric methods, were in the range of 3.047 ± 0.004–5.273 ± 0.013 mg/g and 96.833 ± 0.027–458.833 ± 0.0005 mg/g crude extract of propolis, respectively. This study examined the antioxidant and antimicrobial activities of propolis. Aqueous extracts of propolis were obtained in order to evaluate their antioxidant activities by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, β-carotene and electrochemical assays. All tested propolis samples had relatively strong antioxidant activities, which were also correlated with the total polyphenol and flavonoid content present. The percentage of inhibition of lipid peroxidation of linoleic acid emulsion during 24 h varied between 86.04 ± 0.42 and 90.60 ± 3.77% among the tested samples. The highest DPPH radical scavenging activity was observed by ABAL (Ain Abassa Aqueous Extract) with IC50 = 8.49 ± 5.07 10−5 μg/ml, and the lowest was observed by SAL (Setif Aqueous Extract) with IC50 of 21.16 ± 0.0001 μg/ml. The most important antibacterial activity was obtained with Ain Abassa extract; the zones of inhibition obtained for this excerpt vary from 15.22 to 15.5 mm. Followed by the Setif extract with areas of 12.33 to 12.75 mm, the Tizi-Ouzou extract with areas of 10.11 to 11.11 mm. This study will bring an innovation for further studies with regard to the antioxidant and antibacterial properties of the aqueous extracts of propolis. This study corroborates that Algerian propolis is a rich source of natural antioxidants, properties which could be used in the prevention of different diseases, both in humans and in animals.

scholarly journals ANTIBACTERIAL ACTIVITY AGAINST BACILLUS SUBTILIS AND ANTIOXIDANT PROPERTIES OF METHANOL EXTRACTS FROM GARCINIA LATISSIMA MIQ. LEAVES

2018 ◽  
Vol 10 (1) ◽  
pp. 24 ◽  
Author(s):  
Neneng Siti Silfi Ambarwati ◽  
Berna Elya ◽  
Amarila Malik ◽  
Muhammad Hanafi ◽  
Hanita Omar
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Crude Extract ◽  
Inhibitory Concentration ◽  
Antioxidant Activities ◽  
Wide Spectrum ◽  
Antioxidant Properties ◽  
Antibacterial Activities ◽  
Positive Control ◽  
Inhibition Zones

Objective: The objective of this study was to identify fractions with the highest antibacterial activity against Bacillus subtilis and to determineantioxidant activities and establish the chromatographic fractions as candidate antibacterial and antioxidant agents.Methods: Extracts were fractionated using column chromatography, and antibacterial activities were assayed by the analyses of inhibition zones andbioautography, as well as by broth microdilution techniques. Antioxidant activities were evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH)assay.Results: The strongest antibacterial activity against B. subtilis (ATCC 6633) was observed with fractions B and C obtained in this research, witha minimum inhibitory concentration value of 312.5 μg/mL. The effective percentage (EP) value of crude extract at 10 μg/mL was 29.47±2.01%.Fractions C and D had greater EP values than the crude extract, whereas fraction D had the highest scavenging activity against DPPH free radicals(37.73±1.44%) when used at 10 μg/mL. The half effective concentration of the extract was 23.40 μg/mL, whereas that of the most active fraction Dwas 19.38 μg/mL and quercetin as positive control was 3.72 μg/mL.Conclusion: The present data confirm that fractions of methanol extract from Garcinia latissima Miq. leaves possess antibacterial and antioxidantactivities. These observations may facilitate the development of antimicrobial phytomedicines with a wide spectrum of activities and standardizedantioxidant properties.

Growth Inhibition Effect of Medicinal Herbs Extracts on Bacillus subtilis and Aspergillus oryzae

2016 ◽  
Vol 873 ◽  
pp. 71-78
Author(s):  
Wen Yao Shao ◽  
Xue Shan Pan ◽  
Quan Ling Xie ◽  
Shi Yi Luo ◽  
Xiao Ting Liu
Keyword(s):  
Bacillus Subtilis ◽  
Aspergillus Oryzae ◽  
Minimum Bactericidal Concentration ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Medicinal Herbs ◽  
Soy Sauce ◽  
Rhizoma Coptidis ◽  
Inhibition Effect

With tetracycline and anphotericin as control, using discdiffusion technique to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of extracts from 110 medicinal herbs, investigating their antimicrobial activities. Found that 3 medicinal herbs, Fructus mume, Rhizoma coptidis and Flos caryophyllata have great inhibitory effect on Bacillus subtilis but no obviously inhibitory effect on Aspergillus oryzae. It is evident that the extracts of Fructus mume, Rhizoma coptidis and Flos caryophyllata have antibacterial activities and are potential preservatives in soy sauce fermentation.

Dissecting tunicamycin biosynthesis by genome mining: cloning and heterologous expression of a minimal gene cluster

2010 ◽  
Vol 1 (5) ◽  
pp. 581 ◽  
Author(s):  
Filip J. Wyszynski ◽  
Andrew R. Hesketh ◽  
Mervyn J. Bibb ◽  
Benjamin G. Davis
Keyword(s):  
Heterologous Expression ◽  
Gene Cluster ◽  
Genome Mining

scholarly journals Cytotoxicity, Antimicrobial, and In Silico Studies of Secondary Metabolites From Aspergillus sp. Isolated From Tecoma stans (L.) Juss. Ex Kunth Leaves

2021 ◽  
Vol 9 ◽  
Author(s):  
Heba E. Elsayed ◽  
Reem A. Kamel ◽  
Reham R. Ibrahim ◽  
Ahmed S. Abdel-Razek ◽  
Mohamed A. Shaaban ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
In Silico ◽  
Therapeutic Potential ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Kinase Domain ◽  
Binding Score ◽  
In Silico Studies ◽  
Binding Cleft ◽  
Aspergillus Sp

Endophytes are prolific producers of privileged secondary metabolites with diverse therapeutic potential, although their anticancer and antimicrobial potential still have a room for further investigation. Herein, seven known secondary metabolites namely, arugosin C (1), ergosterol (2), iso-emericellin (3), sterigmatocystin (4), dihydrosterigmatocystin (5), versicolorin B (6), and diorcinol (7) were isolated from the rice culture of Aspergillus sp. retrieved from Tecoma stans (L.) Juss. ex Kunth leaves. Their anticancer and antimicrobial activities were evaluated in MTT and agar well diffusion assays, respectively. The cytotoxicity results showed that metabolite 3 displayed the best viability inhibition on the MCF-7 breast cancer cells with IC50 = 225.21 µM, while 5 on the HepG2 hepatocellular carcinoma cells with IC50 = 161.81 µM. 5 demonstrated a 60% apoptotic mode of cell death which is virtually correlated to its high docking affinity to Hsp90 ATP binding cleft (binding score −8.4 Kcal/mol). On the other side, metabolites 4 and 5 displayed promising antimicrobial activity especially on Pseudomonas aeruginosa with MIC = 125 μg/ml. The observed effect may be likely related to their excellent in silico inhibition of the bacterial DNA-gyrase kinase domain (binding score −10.28 Kcal/mol). To the best of our knowledge, this study is the first to report the promising cytotoxic and antibacterial activities of metabolites 3, 4, and 5 which needs further investigation and renovation to therapeutic leads.

scholarly journals Preliminary Screening of Bacteria Isolated from Insects Living in Poultry for Antibiotic Production

2020 ◽  
pp. 49-57
Author(s):  
Ifeoma Geraldine Okudo ◽  
Chinelo Ursula Umedum ◽  
Stephen Nnaemeka Ezekwueche ◽  
Chibuzo Christian Uba
Keyword(s):  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Antibiotic Production ◽  
Antibacterial Activities ◽  
Dilution Method ◽  
Preliminary Screening ◽  
Agar Plug ◽  
And Staphylococcus Aureus

Aim: This present study was conducted to isolate antibiotic producing bacteria from insects living in poultry. Place and Duration of Study: Insects living in poultry were collected from poultry farms within Onitsha metropolis in Anambra State between April 2018 and September 2018. Methodology: The gut of one hundred insects; (Musca domestica and Alphitobius diaperinus) were analyzed. The insects were dissected and emulsified in 10ml of peptone water. The dilutions were cultured on Nutrient agar and Blood agar  for 24 h. The bacterial isolates were characterized using  molecular identification. The DNA was extracted from the identified isolates and analyzed by 16S rRNA. In preliminary screening, the isolates were inoculated into Muller Hinton agar using agar plug. The promising isolate showing antagonism was subjected to submerged fermentation method and the secondary metabolites were extracted. Screening of the secondary metabolites extract was done using agar well diffusion. The minimum inhibitory concentration (MIC) of the secondary metabolite was determined using broth dilution method at different concentrations. The inhibitory activity of the organism was checked against four bacteria namely; Bacillus subtilis, Salmonella serovar typhi, Escherichia coli and Staphylococcus aureus. Results: The sequence analysis revealed the strains to be Lysinibacillus macroides, Paealcaligenes hermetiae, Bordetella flabilis, Bacillus aerophilus, Klebsiella variicola. Lysinibacillus macroides showed antagonism against the test bacteria during the preliminary test. After fermentation, the secondary metabolite extracts from Lysinibacillus macroides exhibited antibacterial activities against Salmonella Serovar Typhi, Staphyloccus aureus and Bacillus subtilis at different concentrations. Conclusion: The extracts from bacterial isolate; Lysinibacillus macroides exhibited antibacterial activities against Bacillus subtilis, Salmonella serovar typhi and Staphylococcus aureus. The extracts may serve as a new drug molecule produced from natural source when purified.

scholarly journals THÀNH PHẦN VÀ HOẠT TÍNH KHÁNG KHUẨN CỦA CAO ETYL AXETAT CHIẾT TỪ 3 LOÀI RONG ĐỎ THUỘC CHI LAURENCIA

2018 ◽  
Vol 18 (2) ◽  
pp. 187-196
Author(s):  
Le Ho Khanh Hy ◽  
Nguyen Kim Hanh ◽  
Pham Xuan Ky ◽  
Dao Viet Ha ◽  
Nguyen Thu Hong ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Salmonella Typhimurium ◽  
Antibacterial Activities ◽  
Ethyl Acetate Extracts

This paper is concerned with composition and antibacterial activities of ethyl acetate extracts from 3 species of Laurencia (Rhodophyta): Laurencia papillosa, Laurencia flexilis and Laurencia cartilaginea collected from the southern coasts of Vietnam. The results showed that ethyl acetate (EtOAc) extracts of these species displayed antibacterial activities against Salmonella typhimurium, Escherichia coli and Bacillus subtilis. The qualitative investigations revealed the presence of two sesquiterpene secondary metabolites Elatol and Palisadin A in EtOAc extracts from the three above- mentioned Laurencia species.

scholarly journals Genome mining and UHPLC-MS/MS illuminate the specificity of secondary metabolite synthetic gene clusters in Bacillus subtilis NCD-2

2020 ◽  
Author(s):  
Zhenhe Su ◽  
Xiuye Chen ◽  
Xiaomeng Liu ◽  
Qinggang Guo ◽  
Shezeng Li ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Biological Activities ◽  
Genome Mining ◽  
Gene Clusters ◽  
Synthetic Gene ◽  
Integrative Approach ◽  
Amino Acid Sequence Similarity ◽  
Genetic Characteristics

Abstract Background Bacillus subtilisstrain NCD-2 is anexcellent biocontrol agent against plant soil-borne diseases and shows broad-spectrum antifungal activities. This study aimed to explore all the secondary metabolite synthetic gene clusters and related bioactive compounds in NCD-2. An integrative approach, which coupled genome mining with structural identification technologies using ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrometry (UHPLC-MS/MS), was conducted to interpret the chemical origins of the significant biological activities in NCD-2. Results Genome mining revealed that NCD-2 contained nine gene clustershaving predicted functionsinvolving secondary metabolites with bioactive abilities. They encoded six known products-fengycin, surfactin, bacillaene, subtilosin, bacillibactin, and bacilysin-as well as three unknown products.Interestingly, the synthetic gene clusters for surfactin and fengycin showed 83% and 92% amino acid sequence similarity levels with the corresponding productsin Bacillus velezensisstrain FZB42. A further comparison of gene clusters encoding fengycin and surfactinrevealed that strain NCD-2 had lost thefenC and fenDgenes in the fengycinbiosynthetic operon, and that the surfactin synthetic enzyme-related gene srfAB was divided into two parts.Abioinformatics analysis showed that fenEAmay function as fenCD in synthesizing fengycinand that the structure of thisfengycin synthetic gene clusteris likely unique to NCD-2.Five kinds of fengycin,with 26 homologs, and surfactin,with 4 homologs,were detectedfrom strain NCD-2, which indicated the non-typical and unique nature of this fengycin biosynthetic gene cluster.To the best of our knowledge, this is the first report of a non-typical gene cluster related to fengycin synthesis. Conclusions The data provide the genetic characteristics of secondary metabolite synthetic gene clusters for fengycinand surfactin in B. subtilis NCD-2, including the unique synthetic mechanism for fengycin, and suggest that bioactive secondary metabolites explain the biological activities of NCD-2.

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